{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:27:44Z","timestamp":1793039264356,"version":"4.5.0"},"reference-count":68,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T00:00:00Z","timestamp":1692662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Science of The Total Environment"],"published-print":{"date-parts":[[2023,12]]},"DOI":"10.1016\/j.scitotenv.2023.166442","type":"journal-article","created":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T03:03:03Z","timestamp":1692414183000},"page":"166442","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Comparison of concentration and extraction workflows for qPCR quantification of intI1 and vanA in untreated wastewater"],"prefix":"10.1016","volume":"903","author":[{"given":"Yawen","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Metasebia","family":"Gebrewold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart L.","family":"Simpson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Bivins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warish","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.scitotenv.2023.166442_bb0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.138764","article-title":"First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: a proof of concept for the wastewater surveillance of COVID-19 in the community","volume":"728","author":"Ahmed","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0010","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.632850","article-title":"Antibiotic resistance and sewage-associated marker genes in untreated sewage and a river characterized during baseflow and stormflow","volume":"12","author":"Ahmed","year":"2021","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0015","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1016\/j.scitotenv.2017.06.153","article-title":"Sewers as potential reservoirs of antibiotic resistance","volume":"605-606","author":"Auguet","year":"2017","journal-title":"Sci. Total Environ."},{"issue":"3","key":"10.1016\/j.scitotenv.2023.166442_bb0020","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1021\/es404734p","article-title":"Environmental conditions influence eDNA persistence in aquatic systems","volume":"48","author":"Barnes","year":"2014","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0025","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1128\/JCM.36.8.2187-2190.1998","article-title":"Emergence of vancomycin-resistant enterococci in Australia: phenotypic and genotypic characteristics of isolates","volume":"36","author":"Bell","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0030","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1186\/s40168-018-0543-z","article-title":"Quantification of variation and the impact of biomass in targeted 16S rRNA gene sequencing studies","volume":"6","author":"Bender","year":"2018","journal-title":"Microbiome"},{"issue":"5","key":"10.1016\/j.scitotenv.2023.166442_bb0035","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1038\/nrmicro3439","article-title":"Tackling antibiotic resistance: the environmental framework","volume":"13","author":"Berendonk","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"issue":"15","key":"10.1016\/j.scitotenv.2023.166442_bb0040","doi-asserted-by":"crossref","first-page":"10210","DOI":"10.1021\/acs.est.1c01767","article-title":"The Environmental Microbiology Minimum Information (EMMI) guidelines: qPCR and dPCR quality and reporting for environmental microbiology","volume":"55","author":"Borchardt","year":"2021","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0045","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/s43705-021-00079-z","article-title":"Microvolume DNA extraction methods for microscale amplicon and metagenomic studies","volume":"1","author":"Bramucci","year":"2021","journal-title":"ISME Commun."},{"issue":"1","key":"10.1016\/j.scitotenv.2023.166442_bb0050","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.watres.2012.09.044","article-title":"Effect of wastewater colloids on membrane removal of antibiotic resistance genes","volume":"47","author":"Breazeal","year":"2013","journal-title":"Water Res."},{"issue":"4","key":"10.1016\/j.scitotenv.2023.166442_bb0055","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0060","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.watres.2019.06.039","article-title":"Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settings","author":"Cacace","year":"2019","journal-title":"Water Res."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0065","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2020.116592","article-title":"Free-floating extracellular DNA: systematic profiling of mobile genetic elements and antibiotic resistance from wastewater","volume":"189","author":"Calder\u00f3n-Franco","year":"2021","journal-title":"Water Res."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0070","series-title":"Antibiotic Resistance Threats in the United States, 2019","author":"Centers for Disease Control and Prevention (CDC)","year":"2019"},{"issue":"15","key":"10.1016\/j.scitotenv.2023.166442_bb0075","first-page":"8157","article-title":"Effects of advanced treatment systems on the removal of antibiotic resistance genes in wastewater treatment plants from Hangzhou, China","volume":"47","author":"Chen","year":"2013","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0080","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1038\/ismej.2014.8","article-title":"Wastewater as a point source of antibiotic resistance genes in the sediment of a freshwater lake","volume":"8","author":"Czekalski","year":"2014","journal-title":"ISME J."},{"issue":"7365","key":"10.1016\/j.scitotenv.2023.166442_bb0085","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/nature10388","article-title":"Antibiotic resistance is ancient","volume":"477","author":"D\u2019Costa","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.scitotenv.2023.166442_bb0090","doi-asserted-by":"crossref","first-page":"314","DOI":"10.3389\/fmars.2017.00314","article-title":"Evaluation of filtration and DNA extraction methods for environmental DNA biodiversity assessments across multiple trophic levels","volume":"4","author":"Djurhuus","year":"2017","journal-title":"Front. Mar. Sci."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0095","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1093\/jac\/dkh192","article-title":"Class 1 integrons in various Salmonella enterica serovars isolated from animals and identification of genomic island SGI1 in Salmonella enterica var. Melagridis","volume":"53","author":"Ebner","year":"2004","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0100","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1111\/1755-0998.12421","article-title":"Optimizing techniques to capture and extract environmental DNA for detection and quantification of fish","volume":"16","author":"Eichmiller","year":"2016","journal-title":"Mol. Ecol. Resour."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0105","series-title":"A European One Health Action Plan Against Antimicrobial Resistance (AMR)","author":"European Commission","year":"2017"},{"issue":"11","key":"10.1016\/j.scitotenv.2023.166442_bb0110","doi-asserted-by":"crossref","first-page":"6113","DOI":"10.1021\/acs.est.7b05419","article-title":"Metagenomic characterization of antibiotic resistance genes in full-scale reclaimed water distribution systems and corresponding potable systems","volume":"52","author":"Garner","year":"2018","journal-title":"Environ. Sci. Technol."},{"issue":"6","key":"10.1016\/j.scitotenv.2023.166442_bb0115","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1038\/ismej.2014.226","article-title":"Using the class 1 integron-integrase gene as a proxy for anthropogenic pollution","volume":"9","author":"Gillings","year":"2015","journal-title":"ISME J."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0120","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1128\/AAC.45.3.723-726.2001","article-title":"Incidence of class 1 and 2 integrases in clinical and commensal bacteria from livestock, companion animals, and exotics","volume":"45","author":"Goldstein","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0125","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1016\/j.envpol.2018.11.040","article-title":"Occurrence of enterococci harbouring clinically important antibiotic resistance genes in the aquatic environment in Gauteng, South Africa","volume":"245","author":"Hamiwe","year":"2019","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.140466","article-title":"The impact of WWTP size and sampling season on the prevalence of antibiotic resistance genes in wastewater and the river system","volume":"741","author":"Harnisz","year":"2020","journal-title":"Sci. Total Environ."},{"issue":"4","key":"10.1016\/j.scitotenv.2023.166442_bb0135","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.watres.2004.11.011","article-title":"Comparison of Enterococcus measurements in freshwater at two recreational beaches by quantitative polymerase chain reaction and membrane filter culture analysis","volume":"39","author":"Haugland","year":"2005","journal-title":"Water Res."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0140","doi-asserted-by":"crossref","first-page":"13476","DOI":"10.1016\/j.scitotenv.2019.134176","article-title":"The discrepant mobility of antibiotic resistant genes: evidence from their spatial distribution in sewage sludge flocs","volume":"697","author":"He","year":"2019","journal-title":"Sci. Total Environ."},{"issue":"1","key":"10.1016\/j.scitotenv.2023.166442_bb0145","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1038\/s41467-019-08853-3","article-title":"Global monitoring of antimicrobial resistance based on metagenomics analyses of urban sewage","volume":"10","author":"Hendriksen","year":"2019","journal-title":"Nat. Commun."},{"issue":"6","key":"10.1016\/j.scitotenv.2023.166442_bb0150","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0179251","article-title":"Methods to maximize recovery of environmental DNA from water samples","volume":"12","author":"Hinlo","year":"2017","journal-title":"PLoS ONE"},{"issue":"3","key":"10.1016\/j.scitotenv.2023.166442_bb0155","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1002\/ece3.4843","article-title":"Species-level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization","volume":"9","author":"Jeunen","year":"2019","journal-title":"Ecol. Evol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0160","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.envpol.2017.11.048","article-title":"Biomarkers of antibiotic resistance genes during seasonal changes in wastewater treatment systems","volume":"234","author":"Jiao","year":"2018","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0165","doi-asserted-by":"crossref","first-page":"9921","DOI":"10.1002\/ece3.9921","article-title":"Evaluating the sampling effort for the metabarcoding-based detection of fish environmental DNA in the open ocean","volume":"13","author":"Kawakami","year":"2023","journal-title":"Ecol. Evol."},{"issue":"24","key":"10.1016\/j.scitotenv.2023.166442_bb0170","doi-asserted-by":"crossref","first-page":"4395","DOI":"10.1080\/10643389.2021.2024739","article-title":"A framework for standardized qPCR-targets and protocols for quantifying antibiotic resistance in surface water, recycled water and wastewater","volume":"52","author":"Keenum","year":"2022","journal-title":"Crit. Rev. Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0175","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/1755-0998.13107","article-title":"A practical guide to sample preservation and pre-PCR processing of aquatic environmental DNA","volume":"20","author":"Kumar","year":"2020","journal-title":"Mol. Ecol. Resour."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0180","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1093\/femsec\/fix189","article-title":"Effects of sample preservation and DNA extraction on enumeration of antibiotic resistance genes in wastewater","volume":"94","author":"Li","year":"2018","journal-title":"FEMS Microbiol. Ecol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0185","doi-asserted-by":"crossref","first-page":"9324","DOI":"10.1021\/es401342b","article-title":"Filtration recovery of extracellular DNA from environmental water samples","volume":"47","author":"Liang","year":"2013","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0190","doi-asserted-by":"crossref","first-page":"9149","DOI":"10.1021\/acs.est.1c08918","article-title":"Antimicrobial resistance monitoring of water environments: a framework for standardized methods and quality control","volume":"56","author":"Liguori","year":"2022","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0195","series-title":"Standardizing Methods With QA\/QC Standards for Investigating the Occurrence and Removal of Antibiotic Resistant bacteria\/Antibiotic Resistance Genes (ARB\/ARGs) in Surface Water, Wastewater, and Recycled Water","author":"Liguori","year":"2023"},{"issue":"3","key":"10.1016\/j.scitotenv.2023.166442_bb0200","doi-asserted-by":"crossref","first-page":"246","DOI":"10.3390\/w10030246","article-title":"Distribution and abundance of antibiotic resistance genes in sand settling reservoirs and drinking water treatment plants across the Yellow River, China","volume":"10","author":"Lu","year":"2018","journal-title":"Water"},{"issue":"16","key":"10.1016\/j.scitotenv.2023.166442_bb0205","doi-asserted-by":"crossref","first-page":"6102","DOI":"10.1021\/es9038165","article-title":"tet and sul antibiotic resistance genes in livestock lagoons of various operation type, configuration, and antibiotic occurrence","volume":"44","author":"McKinney","year":"2010","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0210","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s10201-015-0457-4","article-title":"Techniques for the practical collection of environmental DNA: filter selection, preservation, and extraction","volume":"17","author":"Minamoto","year":"2016","journal-title":"Limnology"},{"key":"10.1016\/j.scitotenv.2023.166442_bb0215","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.jgar.2014.10.001","article-title":"blaTEM and vanA as indicator genes of antibiotic resistance contamination in a hospital\u2013urban wastewater treatment plant system","volume":"2","author":"Narciso-da-Rocha","year":"2014","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0220","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.146964","article-title":"Monitoring antibiotic resistance genes in wastewater treatment: current strategies and future challenges","volume":"783","author":"Nguyen","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0225","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1038\/21202","article-title":"Emergence of vancomycin tolerance in Streptococcus pneumoniae","volume":"399","author":"Novak","year":"1999","journal-title":"Nature"},{"key":"10.1016\/j.scitotenv.2023.166442_bb0230","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2019.121221","article-title":"Small-scale wastewater treatment plants as a source of the dissemination of antibiotic resistance genes in the aquatic environment","volume":"381","author":"Osi\u0144ska","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0235","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.ijheh.2019.01.004","article-title":"The impact of on-site hospital wastewater treatment on the downstream communal wastewater system in terms of antibiotics and antibiotic resistance genes","volume":"222","author":"Paulus","year":"2019","journal-title":"Int. J. Hyg. Environ. Health"},{"key":"10.1016\/j.scitotenv.2023.166442_bb0240","series-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2020"},{"key":"10.1016\/j.scitotenv.2023.166442_bb0245","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.envpol.2016.10.062","article-title":"Abundance of antibiotic resistance genes in five municipal wastewater treatment plants in the Monastir Governorate, Tunisia","volume":"219","author":"Rafraf","year":"2016","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0250","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0160523","article-title":"An optimized method for quantification of pathogenic Leptospira in environmental water samples","volume":"11","author":"Riediger","year":"2016","journal-title":"PLoS ONE"},{"key":"10.1016\/j.scitotenv.2023.166442_bb5000","doi-asserted-by":"crossref","first-page":"2765","DOI":"10.1128\/AEM.72.4.2765-2774.2006","article-title":"Quantitative PCR targeting 16S rRNA and reductive dehalogenase genes simultaneously monitors multiple Dehalococcoides strains","volume":"72","author":"Ritalahti","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0255","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.scitotenv.2013.01.032","article-title":"Urban wastewater treatment plants as hotspots for antibiotic-resistant bacteria and genes spread into the environment: a review","volume":"447","author":"Rizzo","year":"2013","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0260","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecolind.2020.106194","article-title":"Cell-based internal standard for qPCR determinations of antibiotic resistance indicators in environmental water samples","volume":"113","author":"Rocha","year":"2020","journal-title":"Ecol. Indic."},{"issue":"21","key":"10.1016\/j.scitotenv.2023.166442_bb0265","doi-asserted-by":"crossref","first-page":"4217","DOI":"10.3390\/ijerph16214217","article-title":"Comparison of culture- and quantitative PCR-based indicators of antibiotic resistance in wastewater, recycled water, and tap water","volume":"16","author":"Rocha","year":"2019","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"10.1016\/j.scitotenv.2023.166442_bb0270","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.136499","article-title":"Winter is coming \u2013 impact of temperature on the variation of beta-lactamase and mcr genes in a wastewater treatment plant","volume":"712","author":"Schages","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0275","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2019.135125","article-title":"Membrane-sensitive bacterial DNA extractions and absolute quantitation of recovery efficiencies","volume":"708","author":"Shi","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0280","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1126\/science.1208929","article-title":"Marine microbes see a sea of gradients","volume":"338","author":"Stocker","year":"2012","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.scitotenv.2023.166442_bb0285","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40168-017-0298-y","article-title":"Metagenomics of urban sewage identifies an extensively shared antibiotic resistome in China","volume":"5","author":"Su","year":"2017","journal-title":"Microbiome"},{"key":"10.1016\/j.scitotenv.2023.166442_bb0290","doi-asserted-by":"crossref","first-page":"6803","DOI":"10.1021\/acs.est.5b05398","article-title":"Managing microbial risks from indirect wastewater reuse for irrigation in urbanizing watersheds","volume":"50","author":"Verbyla","year":"2016","journal-title":"Environ. Sci. Technol."},{"issue":"2","key":"10.1016\/j.scitotenv.2023.166442_bb0295","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.mimet.2003.10.014","article-title":"Detection of clinically relevant antibiotic-resistance genes in municipal wastewater using real-time PCR (TaqMan)","volume":"56","author":"Volkmann","year":"2004","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.scitotenv.2023.166442_bb0300","series-title":"Monitoring and Evaluation of the Global Action Plan on Antimicrobial Resistance: Framework and Recommended Indicators","author":"World Health Organization (WHO)","year":"2019"},{"key":"10.1016\/j.scitotenv.2023.166442_bb0305","article-title":"New estimation of antibiotic resistance genes in sediment along the Haihe River and Bohai Bay in China: a comparison between single and successive DNA extraction methods","volume":"12","author":"Wu","year":"2021","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0310","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.watres.2014.05.019","article-title":"Fate of antibiotic resistance genes in sewage treatment plant revealed by metagenomic approach","volume":"62","author":"Yang","year":"2014","journal-title":"Water Res."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0315","doi-asserted-by":"crossref","first-page":"9713","DOI":"10.1021\/acs.est.3c00159","article-title":"Toward a universal unit for quantification of antibiotic resistance genes in environmental samples","volume":"57","author":"Yin","year":"2023","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0320","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1111\/2041-210X.13879","article-title":"Assessing the performance and efficiency of environmental DNA\/RNA capture methodologies under controlled experimental conditions","volume":"13","author":"Zaiko","year":"2022","journal-title":"Methods Ecol. Evol."},{"issue":"8","key":"10.1016\/j.scitotenv.2023.166442_bb0325","doi-asserted-by":"crossref","first-page":"814","DOI":"10.3390\/v12080814","article-title":"Assessment of metagenomic sequencing and qPCR for detection of influenza D virus in bovine respiratory tract samples","volume":"12","author":"Zhang","year":"2020","journal-title":"Viruses"},{"key":"10.1016\/j.scitotenv.2023.166442_bb0330","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1021\/acs.est.7b04283","article-title":"Cell-free DNA: a neglected source for antibiotic resistance genes spreading from WWTPs","volume":"52","author":"Zhang","year":"2018","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2023.166442_bb0335","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2020.105914","article-title":"The prevalence of drug-resistant and virulent Staphylococcus spp. in a municipal wastewater treatment plant and their spread in the environment","volume":"143","author":"Zieli\u0144ski","year":"2020","journal-title":"Environ. Int."}],"container-title":["Science of The Total Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0048969723050672?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0048969723050672?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T13:47:35Z","timestamp":1787665655000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0048969723050672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12]]},"references-count":68,"alternative-id":["S0048969723050672"],"URL":"https:\/\/doi.org\/10.1016\/j.scitotenv.2023.166442","relation":{},"ISSN":["0048-9697"],"issn-type":[{"value":"0048-9697","type":"print"}],"subject":[],"published":{"date-parts":[[2023,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparison of concentration and extraction workflows for qPCR quantification of intI1 and vanA in untreated wastewater","name":"articletitle","label":"Article Title"},{"value":"Science of The Total Environment","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.scitotenv.2023.166442","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"166442"}}