{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T10:11:40Z","timestamp":1778667100992,"version":"3.51.4"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T00:00:00Z","timestamp":1747699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"publisher","award":["315266"],"award-info":[{"award-number":["315266"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016931","name":"Institute of Marine Research","doi-asserted-by":"publisher","award":["15930"],"award-info":[{"award-number":["15930"]}],"id":[{"id":"10.13039\/100016931","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Science of The Total Environment"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1016\/j.scitotenv.2025.179699","type":"journal-article","created":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T11:14:59Z","timestamp":1748258099000},"page":"179699","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Characterization of known and novel clinically important antibiotic resistance genes and novel microbes from wastewater-impacted high Arctic fjord sediments"],"prefix":"10.1016","volume":"985","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1409-5894","authenticated-orcid":false,"given":"Manish P.","family":"Victor","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lise","family":"\u00d8vre\u00e5s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2955-3402","authenticated-orcid":false,"given":"Nachiket P.","family":"Marathe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"6","key":"10.1016\/j.scitotenv.2025.179699_bb0005","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1111\/1755-0998.12399","article-title":"METAXA2: improved identification and taxonomic classification of small and large subunit rRNA in metagenomic data","volume":"15","author":"Bengtsson-Palme","year":"2015","journal-title":"Mol. Ecol. Resour."},{"key":"10.1016\/j.scitotenv.2025.179699_bb0010","doi-asserted-by":"crossref","DOI":"10.1038\/s41587-023-01983-6","article-title":"High-quality metagenome assembly from long accurate reads with metaMDBG","author":"Benoit","year":"2024","journal-title":"Nat. Biotechnol."},{"issue":"1","key":"10.1016\/j.scitotenv.2025.179699_bb0015","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s40168-019-0670-1","article-title":"Identification and reconstruction of novel antibiotic resistance genes from metagenomes","volume":"7","author":"Berglund","year":"2019","journal-title":"Microbiome"},{"issue":"1","key":"10.1016\/j.scitotenv.2025.179699_bb0025","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1186\/s40168-017-0353-8","article-title":"Identification of 76 novel B1 metallo-\u03b2-lactamases through large-scale screening of genomic and metagenomic data","volume":"5","author":"Berglund","year":"2017","journal-title":"Microbiome"},{"issue":"4","key":"10.1016\/j.scitotenv.2025.179699_bb0030","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1038\/s41592-021-01101-x","article-title":"Sensitive protein alignments at tree-of-life scale using DIAMOND","volume":"18","author":"Buchfink","year":"2021","journal-title":"Nat. Methods"},{"issue":"9","key":"10.1016\/j.scitotenv.2025.179699_bb0035","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/s41579-019-0222-5","article-title":"Scientists\u2019 warning to humanity: microorganisms and climate change","volume":"17","author":"Cavicchioli","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.scitotenv.2025.179699_bb1000","doi-asserted-by":"crossref","first-page":"D325","DOI":"10.1093\/nar\/gki008","article-title":"VFDB: a reference database for bacterial virulence factors","volume":"33","author":"Chen","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.scitotenv.2025.179699_bb0040","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkn180","article-title":"Phylogeny.fr: robust phylogenetic analysis for the non-specialist","volume":"36","author":"Dereeper","year":"2008","journal-title":"Nucleic Acids Res"},{"issue":"D1","key":"10.1016\/j.scitotenv.2025.179699_bb0045","doi-asserted-by":"crossref","first-page":"D1168","DOI":"10.1093\/nar\/gkr1144","article-title":"MitoZoa 2.0: a database resource and search tools for comparative and evolutionary analyses of mitochondrial genomes in Metazoa","volume":"40","author":"D\u2019Onorio De Meo","year":"2012","journal-title":"Nucleic Acids Res."},{"issue":"10","key":"10.1016\/j.scitotenv.2025.179699_bb0050","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1002195","article-title":"Accelerated profile HMM searches","volume":"7","author":"Eddy","year":"2011","journal-title":"PLoS Comput. Biol."},{"issue":"19","key":"10.1016\/j.scitotenv.2025.179699_bb0055","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search and clustering orders of magnitude faster than BLAST","volume":"26","author":"Edgar","year":"2010","journal-title":"Bioinformatics"},{"issue":"5","key":"10.1016\/j.scitotenv.2025.179699_bb0060","article-title":"Microbial genomics amidst the arctic crisis","volume":"6","author":"Edwards","year":"2020","journal-title":"Microb. Genom."},{"issue":"1","key":"10.1016\/j.scitotenv.2025.179699_bb0065","doi-asserted-by":"crossref","first-page":"12728","DOI":"10.1038\/s41598-021-91456-0","article-title":"AMRFinderPlus and the reference gene catalog facilitate examination of the genomic links among antimicrobial resistance, stress response, and virulence","volume":"11","author":"Feldgarden","year":"2021","journal-title":"Sci. Rep."},{"issue":"7502","key":"10.1016\/j.scitotenv.2025.179699_bb0070","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1038\/nature13377","article-title":"Bacterial phylogeny structures soil resistomes across habitats","volume":"509","author":"Forsberg","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.scitotenv.2025.179699_bb0075","article-title":"Local contamination in Svalbard: overview and suggestions for remediation actions","author":"Granberg","year":"2017","journal-title":"Norsk Polarinstitutt"},{"issue":"19","key":"10.1016\/j.scitotenv.2025.179699_bb0080","doi-asserted-by":"crossref","first-page":"2811","DOI":"10.1093\/bioinformatics\/btu393","article-title":"Circlize implements and enhances circular visualization in R","volume":"30","author":"Gu","year":"2014","journal-title":"Bioinformatics"},{"issue":"1","key":"10.1016\/j.scitotenv.2025.179699_bb0085","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1128\/AAC.01310-13","article-title":"ARG-ANNOT, a new Bioinformatic tool to discover antibiotic resistance genes in bacterial genomes","volume":"58","author":"Gupta","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"issue":"D1","key":"10.1016\/j.scitotenv.2025.179699_bb0090","doi-asserted-by":"crossref","first-page":"D566","DOI":"10.1093\/nar\/gkw1004","article-title":"CARD 2017: expansion and model-centric curation of the comprehensive antibiotic resistance database","volume":"45","author":"Jia","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.scitotenv.2025.179699_bb2000","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1038\/ismej.2016.174","article-title":"Where less may be more: how the rare biosphere pulls ecosystems strings","volume":"11","author":"Jousset","year":"2017","journal-title":"ISME J"},{"issue":"3","key":"10.1016\/j.scitotenv.2025.179699_bb0095","doi-asserted-by":"crossref","DOI":"10.3390\/w12030794","article-title":"Heavy metals in a high arctic fiord and their introduction with the wastewater: a case study of Adventfjorden-Longyearbyen system, Svalbard","volume":"12","author":"Kalinowska","year":"2020","journal-title":"Water"},{"key":"10.1016\/j.scitotenv.2025.179699_bb0100","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.7359","article-title":"MetaBAT 2: an adaptive binning algorithm for robust and efficient genome reconstruction from metagenome assemblies","volume":"7","author":"Kang","year":"2019","journal-title":"PeerJ"},{"issue":"14","key":"10.1016\/j.scitotenv.2025.179699_bb0105","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1093\/nar\/gkf436","article-title":"MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform","volume":"30","author":"Katoh","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.scitotenv.2025.179699_bb0110","doi-asserted-by":"crossref","DOI":"10.3389\/fevo.2017.00137","article-title":"Pole-to-pole connections: similarities between Arctic and Antarctic microbiomes and their vulnerability to environmental change","volume":"5","author":"Kleinteich","year":"2017","journal-title":"Front. Ecol. Evol."},{"issue":"10","key":"10.1016\/j.scitotenv.2025.179699_bb0115","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1093\/bioinformatics\/btv033","article-title":"MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph","volume":"31","author":"Li","year":"2015","journal-title":"Bioinformatics"},{"issue":"1","key":"10.1016\/j.scitotenv.2025.179699_bb0120","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s40168-019-0710-x","article-title":"Sewage effluent from an Indian hospital harbors novel carbapenemases and integron-borne antibiotic resistance genes","volume":"7","author":"Marathe","year":"2019","journal-title":"Microbiome"},{"issue":"4","key":"10.1016\/j.scitotenv.2025.179699_bb0125","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1007\/s12275-018-8014-6","article-title":"UBCG: up-to-date bacterial core gene set and pipeline for phylogenomic tree reconstruction","volume":"56","author":"Na","year":"2018","journal-title":"J. Microbiol."},{"issue":"1","key":"10.1016\/j.scitotenv.2025.179699_bb0130","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1080\/14756366.2017.1344235","article-title":"Beta-lactamase database (BLDB) \u2013 structure and function","volume":"32","author":"Naas","year":"2017","journal-title":"J. Enzyme Inhib. Med. Chem."},{"issue":"D1","key":"10.1016\/j.scitotenv.2025.179699_bb0135","doi-asserted-by":"crossref","first-page":"D737","DOI":"10.1093\/nar\/gkt1252","article-title":"BacMet: antibacterial biocide and metal resistance genes database","volume":"42","author":"Pal","year":"2014","journal-title":"Nucleic Acids Res."},{"issue":"7","key":"10.1016\/j.scitotenv.2025.179699_bb0140","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1101\/gr.186072.114","article-title":"CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes","volume":"25","author":"Parks","year":"2015","journal-title":"Genome Res."},{"issue":"1","key":"10.1016\/j.scitotenv.2025.179699_bb0145","doi-asserted-by":"crossref","first-page":"3891","DOI":"10.1038\/s41467-018-06393-w","article-title":"Maternal gut and breast milk microbiota affect infant gut antibiotic resistome and mobile genetic elements","volume":"9","author":"P\u00e4rn\u00e4nen","year":"2018","journal-title":"Nat. Commun."},{"issue":"3","key":"10.1016\/j.scitotenv.2025.179699_bb0150","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0009490","article-title":"FastTree 2 \u2013 approximately maximum-likelihood trees for large alignments","volume":"5","author":"Price","year":"2010","journal-title":"PLoS One"},{"issue":"D1","key":"10.1016\/j.scitotenv.2025.179699_bb0155","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: improved data processing and web-based tools","volume":"41","author":"Quast","year":"2012","journal-title":"Nucleic Acids Res."},{"issue":"6","key":"10.1016\/j.scitotenv.2025.179699_bb0160","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1093\/bioinformatics\/btr026","article-title":"Quality control and preprocessing of metagenomic datasets","volume":"27","author":"Schmieder","year":"2011","journal-title":"Bioinformatics"},{"issue":"14","key":"10.1016\/j.scitotenv.2025.179699_bb0165","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.scitotenv.2025.179699_bb0170","doi-asserted-by":"crossref","unstructured":"Sharifi, F. and Ye, Y. \u2018From Gene Annotation to Function Prediction for Metagenomics\u2019. Kihara, D. Protein Function Prediction, vol. 1611, Springer New York, New York, NY, 2017, pp. 27\u201334. [Online]. Available: http:\/\/link.springer.com\/10.1007\/978-1-4939-7015-5_3.","DOI":"10.1007\/978-1-4939-7015-5_3"},{"key":"10.1016\/j.scitotenv.2025.179699_bb0175","doi-asserted-by":"crossref","first-page":"3349","DOI":"10.3389\/fmicb.2018.03349","article-title":"SqueezeMeta, a highly portable, fully automatic metagenomic analysis pipeline","volume":"9","author":"Tamames","year":"2019","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.scitotenv.2025.179699_bb0180","doi-asserted-by":"crossref","unstructured":"Verde, C., Giordano, D., Bellas, C. M., Di Prisco, G., and Anesio, A. M. \u2018Polar marine microorganisms and climate change\u2019, in Advances in microbial physiology, vol. 69, Elsevier, 2016, pp. 187\u2013215. [Online]. Available: https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0065291116300236.","DOI":"10.1016\/bs.ampbs.2016.07.002"},{"issue":"4","key":"10.1016\/j.scitotenv.2025.179699_bb0185","doi-asserted-by":"crossref","DOI":"10.1128\/mra.01063-23","article-title":"Updated RDP taxonomy and RDP classifier for more accurate taxonomic classification","volume":"13","author":"Wang","year":"2024","journal-title":"Microbiol. Resour. Announc."},{"issue":"5","key":"10.1016\/j.scitotenv.2025.179699_bb0190","doi-asserted-by":"crossref","DOI":"10.1093\/femsec\/fiaa057","article-title":"Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost","volume":"96","author":"Xue","year":"2020","journal-title":"FEMS Microbiol. Ecol."},{"issue":"1","key":"10.1016\/j.scitotenv.2025.179699_bb0195","doi-asserted-by":"crossref","first-page":"4765","DOI":"10.1038\/s41467-021-25096-3","article-title":"An omics-based framework for assessing the health risk of antimicrobial resistance genes","volume":"12","author":"Zhang","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.scitotenv.2025.179699_bb0200","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.151937","article-title":"Metagenomic analysis reveals microbiome and resistome in the seawater and sediments of Kongsfjorden (Svalbard, high Arctic)","volume":"809","author":"Zhang","year":"2022","journal-title":"Sci. Total Environ."}],"container-title":["Science of The Total Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0048969725013403?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0048969725013403?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T09:22:37Z","timestamp":1778664157000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0048969725013403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":41,"alternative-id":["S0048969725013403"],"URL":"https:\/\/doi.org\/10.1016\/j.scitotenv.2025.179699","relation":{},"ISSN":["0048-9697"],"issn-type":[{"value":"0048-9697","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of known and novel clinically important antibiotic resistance genes and novel microbes from wastewater-impacted high Arctic fjord sediments","name":"articletitle","label":"Article Title"},{"value":"Science of The Total Environment","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.scitotenv.2025.179699","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"179699"}}