{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T04:29:46Z","timestamp":1763612986491,"version":"3.45.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T00:00:00Z","timestamp":1760832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005365","name":"Ehime University","doi-asserted-by":"publisher","award":["23-34","24-39"],"award-info":[{"award-number":["23-34","24-39"]}],"id":[{"id":"10.13039\/501100005365","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006120","name":"Ministry of the Environment, Government of Japan","doi-asserted-by":"publisher","award":["5-1952","5-2204"],"award-info":[{"award-number":["5-1952","5-2204"]}],"id":[{"id":"10.13039\/501100006120","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014423","name":"Environmental Restoration and Conservation Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014423","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17H06432"],"award-info":[{"award-number":["17H06432"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018502","name":"Kanazawa University","doi-asserted-by":"publisher","award":["23020"],"award-info":[{"award-number":["23020"]}],"id":[{"id":"10.13039\/100018502","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017993","name":"Institute of Nature and Environmental Technology, Kanazawa University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017993","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004087","name":"Shizuoka University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004087","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000277","name":"Department for Environment, Food and Rural Affairs, UK Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000277","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["21H02522","25H01183"],"award-info":[{"award-number":["21H02522","25H01183"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007106","name":"Gunma University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007106","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004206","name":"Osaka University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004206","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Science of The Total Environment"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.scitotenv.2025.180773","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T10:07:41Z","timestamp":1761041261000},"page":"180773","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Identification of the estrogen receptor subtypes associated with testis-ova induction in the medaka"],"prefix":"10.1016","volume":"1003","author":[{"given":"Shuhei","family":"Murota","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiwako","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiraku","family":"Nakashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suika","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sayaka","family":"Enomoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reimi","family":"Tambo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sho","family":"Yamazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiko","family":"Ogino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anke","family":"Lange","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles R.","family":"Tyler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taisen","family":"Iguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichi","family":"Miyagawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.scitotenv.2025.180773_bb0005","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1242\/bio.20148177","article-title":"Targeted mutagenesis using CRISPR\/Cas system in medaka","volume":"3","author":"Ansai","year":"2014","journal-title":"Biol. Open."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0010","doi-asserted-by":"crossref","first-page":"12632","DOI":"10.1021\/acs.est.3c02854","article-title":"Endocrine disruption is reduced but still widespread in wild roach (Rutilus rutilus) living in English rivers","volume":"57","author":"Baynes","year":"2023","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0015","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.jsbmb.2010.11.015","article-title":"Estrogen receptors in medaka (Oryzias latipes) and estrogenic environmental contaminants: an in vitro-in vivo correlation","volume":"123","author":"Chakraborty","year":"2011","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0020","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.mce.2010.12.002","article-title":"Differential expression of three estrogen receptor subtype mRNAs in gonads and liver from embryos to adults of the medaka, Oryzias latipes","volume":"333","author":"Chakraborty","year":"2011","journal-title":"Mol. Cell Endocrinol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0025","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.stemcr.2019.07.013","article-title":"Estrogen receptor beta2 oversees germ cell maintenance and gonadal sex differentiation in medaka, Oryzias latipes","volume":"13","author":"Chakraborty","year":"2019","journal-title":"Stem Cell Reports."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0030","doi-asserted-by":"crossref","first-page":"287","DOI":"10.3109\/10408440903373590","article-title":"Pharmaceuticals in the aquatic environment: a critical review of the evidence for health effects in fish","volume":"40","author":"Corcoran","year":"2010","journal-title":"Crit. Rev. Toxicol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0035","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1021\/es9707973","article-title":"Identification of estrogenic chemicals in STW effluent. 1. chemical fractionation and in vitro biological screening","volume":"32","author":"Desbrow","year":"1998","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0040","first-page":"353","article-title":"Production of testis-ova in adult males of Oryzias latipes I. Testis-ova in the fish receiving estrogens","volume":"11","author":"Egami","year":"1955","journal-title":"Jpn. J. Zool."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0045","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1002\/etc.5620180834","article-title":"Factors affecting the development of testis-ova in medaka, Oryzias latipes, exposed to octylphenol","volume":"18","author":"Gray","year":"1999","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0050","doi-asserted-by":"crossref","first-page":"10751","DOI":"10.1073\/pnas.97.20.10751","article-title":"Identification of a third distinct estrogen receptor and reclassification of estrogen receptors in teleosts","volume":"97","author":"Hawkins","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0055","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.bbrc.2015.09.112","article-title":"Mutation of gonadal soma-derived factor induces medaka XY gonads to undergo ovarian development","volume":"467","author":"Imai","year":"2015","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0060","doi-asserted-by":"crossref","first-page":"607","DOI":"10.2108\/zsj.20.607","article-title":"Normal growth of the \u201csee-through\u201d medaka","volume":"20","author":"Iwamatsu","year":"2003","journal-title":"Zoolog. Sci."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0065","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1002\/jez.a.120","article-title":"Estradiol-17beta content in developing eggs and induced sex reversal of the medaka (Oryzias latipes)","volume":"303","author":"Iwamatsu","year":"2005","journal-title":"J. Exp. Zool. A Comp. Exp. Biol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0070","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1021\/es9710870","article-title":"Widespread sexual disruption in wild fish","volume":"32","author":"Jobling","year":"1998","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0075","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1095\/biolreprod67.2.515","article-title":"Wild intersex roach (Rutilus rutilus) have reduced fertility","volume":"67","author":"Jobling","year":"2002","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0080","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1289\/ehp.8050","article-title":"Predicted exposures to steroid estrogens in U.K. rivers correlate with widespread sexual disruption in wild fish populations","volume":"114 Suppl 1","author":"Jobling","year":"2006","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0085","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1002\/(SICI)1098-2795(200001)55:1<31::AID-MRD5>3.0.CO;2-7","article-title":"Systematic identification of genes expressed during early oogenesis in medaka","volume":"55","author":"Kanamori","year":"2000","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0090","doi-asserted-by":"crossref","first-page":"8868","DOI":"10.1038\/s41598-019-45373-y","article-title":"Gene knockout analysis reveals essentiality of estrogen receptor beta1 (Esr2a) for female reproduction in medaka","volume":"9","author":"Kayo","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0095","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1002\/mrd.20942","article-title":"Transgenic medaka enables easy oocytes detection in live fish","volume":"76","author":"Kinoshita","year":"2009","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0100","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.aquatox.2017.08.011","article-title":"Estrogen alters gonadal soma-derived factor (Gsdf)\/Foxl2 expression levels in the testes associated with testis-ova differentiation in adult medaka, Oryzias latipes","volume":"191","author":"Kobayashi","year":"2017","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0105","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1021\/es802661p","article-title":"Sexual reprogramming and estrogenic sensitization in wild fish exposed to ethinylestradiol","volume":"43","author":"Lange","year":"2009","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0110","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1021\/es103232q","article-title":"Implications of persistent exposure to treated wastewater effluent for breeding in wild roach (Rutilus rutilus) populations","volume":"45","author":"Lange","year":"2011","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0115","doi-asserted-by":"crossref","first-page":"2292","DOI":"10.1210\/en.2017-00215","article-title":"Functional analysis of nuclear estrogen receptors in zebrafish reproduction by genome editing approach","volume":"158","author":"Lu","year":"2017","journal-title":"Endocrinology"},{"key":"10.1016\/j.scitotenv.2025.180773_bb0120","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s10577-011-9264-x","article-title":"Dmrt1 mutation causes a male-to-female sex reversal after the sex determination by Dmy in the medaka","volume":"20","author":"Masuyama","year":"2012","journal-title":"Chromosome Res."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0125","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1002\/etc.5620200210","article-title":"Estrogenic potency of chemicals detected in sewage treatment plant effluents as determined by in vivo assays with Japanese medaka (Oryzias latipes)","volume":"20","author":"Metcalfe","year":"2001","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0130","doi-asserted-by":"crossref","first-page":"2050","DOI":"10.1073\/pnas.94.5.2050","article-title":"Cloning of cDNA and estrogen-induced hepatic gene expression for choriogenin H, a precursor protein of the fish egg envelope (chorion)","volume":"94","author":"Murata","year":"1997","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0135","doi-asserted-by":"crossref","first-page":"425","DOI":"10.2108\/zs180194","article-title":"Inter- and intraspecific variation in sex hormone-induced sex-reversal in Medaka, Oryzias latipes and Oryzias sakaizumii","volume":"36","author":"Myosho","year":"2019","journal-title":"Zoolog. Sci."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.cub.2021.01.089","article-title":"Estrogen receptor 2b is the major determinant of sex-typical mating behavior and sexual preference in medaka","volume":"31","author":"Nishiike","year":"2021","journal-title":"Curr. Biol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0145","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jsbmb.2018.06.002","article-title":"Functional distinctions associated with the diversity of sex steroid hormone receptors ESR and AR","volume":"184","author":"Ogino","year":"2018","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0150","doi-asserted-by":"crossref","first-page":"653","DOI":"10.3109\/10408444.2012.692114","article-title":"Endocrine disrupting chemicals and sexual behaviors in fish\u2014a critical review on effects and possible consequences","volume":"42","author":"Soffker","year":"2012","journal-title":"Crit. Rev. Toxicol."},{"issue":"Suppl. 7","key":"10.1016\/j.scitotenv.2025.180773_bb0155","first-page":"173","article-title":"Vitellogenesis as a biomarker for estrogenic contamination of the aquatic environment","volume":"103","author":"Sumpter","year":"1995","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0160","doi-asserted-by":"crossref","first-page":"7439","DOI":"10.1021\/acs.est.5b00704","article-title":"Understanding the molecular basis for differences in responses of fish estrogen receptor subtypes to environmental estrogens","volume":"49","author":"Tohyama","year":"2015","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0165","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.jsbmb.2015.12.009","article-title":"Evolution of estrogen receptors in ray-finned fish and their comparative responses to estrogenic substances","volume":"158","author":"Tohyama","year":"2016","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0170","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1111\/dgd.12386","article-title":"Establishment of estrogen receptor 1 (ESR1)-knockout medaka: ESR1 is dispensable for sexual development and reproduction in medaka, Oryzias latipes","volume":"59","author":"Tohyama","year":"2017","journal-title":"Dev. Growth Differ."},{"key":"10.1016\/j.scitotenv.2025.180773_bb0175","first-page":"211","article-title":"Medaka (Oryzias latipes) for use in evaluating developmental effects of endocrine active chemicals with special reference to gonadal intersex (testis-ova)","volume":"14","author":"Urushitani","year":"2007","journal-title":"Environ. Sci."}],"container-title":["Science of The Total Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0048969725024131?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0048969725024131?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T04:05:59Z","timestamp":1763611559000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0048969725024131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":35,"alternative-id":["S0048969725024131"],"URL":"https:\/\/doi.org\/10.1016\/j.scitotenv.2025.180773","relation":{},"ISSN":["0048-9697"],"issn-type":[{"type":"print","value":"0048-9697"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification of the estrogen receptor subtypes associated with testis-ova induction in the medaka","name":"articletitle","label":"Article Title"},{"value":"Science of The Total Environment","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.scitotenv.2025.180773","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"180773"}}