{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:39:52Z","timestamp":1792784392305,"version":"4.5.0"},"reference-count":33,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,7,22]],"date-time":"2015-07-22T00:00:00Z","timestamp":1437523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Virtual Institute","award":["VH-VI-510"],"award-info":[{"award-number":["VH-VI-510"]}]},{"DOI":"10.13039\/501100002347","name":"BMBF","doi-asserted-by":"publisher","award":["01GI0704"],"award-info":[{"award-number":["01GI0704"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG BO1718\/4-1"],"award-info":[{"award-number":["DFG BO1718\/4-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003042","name":"Else-Kr\u00f6ner-Fresenius-Stiftung","doi-asserted-by":"crossref","award":["2011 A200"],"award-info":[{"award-number":["2011 A200"]}],"id":[{"id":"10.13039\/501100003042","id-type":"DOI","asserted-by":"crossref"}]},{"name":"European Community's Health Seventh Framework Programme","award":["259867"],"award-info":[{"award-number":["259867"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Stem Cell Research"],"published-print":{"date-parts":[[2015,9]]},"DOI":"10.1016\/j.scr.2015.07.005","type":"journal-article","created":{"date-parts":[[2015,7,26]],"date-time":"2015-07-26T09:53:39Z","timestamp":1437904419000},"page":"328-336","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":95,"title":["Formation and characterisation of neuromuscular junctions between hiPSC derived motoneurons and myotubes"],"prefix":"10.1016","volume":"15","author":[{"given":"M.","family":"Demestre","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Orth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.J.","family":"F\u00f6hr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Achberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.C.","family":"Ludolph","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Liebau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.M.","family":"Boeckers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.scr.2015.07.005_bb0005","first-page":"149","article-title":"Myogenic differentiation of muscular dystrophy-specific induced pluripotent stem cells for use in drug discovery","volume":"3","author":"Abujarour","year":"2014","journal-title":"Stem"},{"key":"10.1016\/j.scr.2015.07.005_bb0010","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1083\/jcb.151.6.1221","article-title":"Expression of CD34 and Myf5 defines the majority of quiescent adult skeletal muscle satellite cells","volume":"151","author":"Beauchamp","year":"2000","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.scr.2015.07.005_bb0015","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1002\/stem.700","article-title":"Concise review: induced pluripotent stem cells versus embryonic stem cells: close enough or yet too far apart?","volume":"30","author":"Bilic","year":"2012","journal-title":"Stem Cells"},{"key":"10.1016\/j.scr.2015.07.005_bb0025","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.stem.2012.02.015","article-title":"Human ES- and iPS-derived myogenic progenitors restore DYSTROPHIN and improve contractility upon transplantation in dystrophic mice","volume":"10","author":"Darabi","year":"2012","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.scr.2015.07.005_bb0030","first-page":"719","article-title":"Motoneuron firing in amyotrophic lateral sclerosis (ALS)","volume":"8","author":"de Carvalho","year":"2014","journal-title":"Front"},{"key":"10.1016\/j.scr.2015.07.005_bb0035","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1111\/j.1365-2990.2005.00676.x","article-title":"Characterization of matrix metalloproteinases in denervated muscle","volume":"31","author":"Demestre","year":"2005","journal-title":"Neuropathol. Appl. Neurobiol."},{"key":"10.1016\/j.scr.2015.07.005_bb0040","doi-asserted-by":"crossref","first-page":"353","DOI":"10.3109\/08916930903555943","article-title":"Antibody effector mechanisms in myasthenia gravis-pathogenesis at the neuromuscular junction","volume":"43","author":"Gomez","year":"2010","journal-title":"Autoimmunity"},{"key":"10.1016\/j.scr.2015.07.005_bb0045","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1038\/mt.2012.188","article-title":"Myoblasts derived from normal hESCs and dystrophic hiPSCs efficiently fuse with existing muscle fibers following transplantation","volume":"20","author":"Goudenege","year":"2012","journal-title":"Mol. Ther."},{"key":"10.1016\/j.scr.2015.07.005_bb0050","doi-asserted-by":"crossref","first-page":"9602","DOI":"10.1016\/j.biomaterials.2011.09.014","article-title":"Neuromuscular junction formation between human stem cell-derived motoneurons and human skeletal muscle in a defined system","volume":"32","author":"Guo","year":"2011","journal-title":"Biomaterials"},{"key":"10.1016\/j.scr.2015.07.005_bb0055","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1039\/C3BM60166H","article-title":"In vitro differentiation of functional human skeletal myotubes in a defined system","volume":"2","author":"Guo","year":"2013","journal-title":"Biomaterials"},{"key":"10.1016\/j.scr.2015.07.005_bb0060","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/BF00656997","article-title":"Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches","volume":"391","author":"Hamill","year":"1981","journal-title":"Pflugers Arch."},{"key":"10.1016\/j.scr.2015.07.005_bb0065","article-title":"Derivation of myogenic progenitors directly from human pluripotent stem cells using a sphere-based culture","volume":"3","author":"Hosoyama","year":"2014","journal-title":"Stem"},{"key":"10.1016\/j.scr.2015.07.005_bb0070","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1038\/nprot.2009.127","article-title":"Differentiation of spinal motor neurons from pluripotent human stem cells","volume":"4","author":"Hu","year":"2009","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.scr.2015.07.005_bb0075","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1155\/2013\/360573","article-title":"Definitive endoderm formation from plucked human hair-derived induced pluripotent stem cells and SK channel regulation","volume":"2013","author":"Illing","year":"2013","journal-title":"Stem Cells Int."},{"key":"10.1016\/j.scr.2015.07.005_bb0080","doi-asserted-by":"crossref","first-page":"4361","DOI":"10.1242\/jcs.00110","article-title":"The role of CD34 expression and cellular fusion in the regeneration capacity of myogenic progenitor cells","volume":"115","author":"Jankowski","year":"2002","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.scr.2015.07.005_bb0085","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1089\/scd.2011.0026","article-title":"Rat embryonic fibroblasts improve reprogramming of human keratinocytes into induced pluripotent stem cells","volume":"21","author":"Linta","year":"2011","journal-title":"Stem Cells Dev."},{"key":"10.1016\/j.scr.2015.07.005_bb0090","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/978-1-62703-317-6_6","article-title":"Human myoblasts from skeletal muscle biopsies: in vitro culture preparations for morphological and cytochemical analyses at light and electron microscopy","volume":"976","author":"Malatesta","year":"2013","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.scr.2015.07.005_bb0095","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.stem.2011.02.004","article-title":"Mutant human embryonic stem cells reveal neurite and synapse formation defects in type 1 myotonic dystrophy","volume":"8","author":"Marteyn","year":"2011","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.scr.2015.07.005_bb0100","doi-asserted-by":"crossref","first-page":"3645","DOI":"10.1523\/JNEUROSCI.09-10-03645.1989","article-title":"Characterization of two factors enhancing choline acetyltransferase activity in cultures of purified rat motoneurons","volume":"9","author":"Martinou","year":"1989","journal-title":"J. Neurosci."},{"key":"10.1016\/j.scr.2015.07.005_bb0105","doi-asserted-by":"crossref","first-page":"19546","DOI":"10.1073\/pnas.0609411103","article-title":"Gene transfer demonstrates that muscle is not a primary target for non-cell-autonomous toxicity in familial amyotrophic lateral sclerosis","volume":"103","author":"Miller","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.scr.2015.07.005_bb0110","first-page":"252","article-title":"ALS as a distal axonopathy: molecular mechanisms affecting neuromuscular junction stability in the presymptomatic stages of the disease","volume":"8","author":"Moloney","year":"2014","journal-title":"Front"},{"key":"10.1016\/j.scr.2015.07.005_bb0115","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/j.1365-2990.2010.01061.x","article-title":"Review: neuromuscular synaptic vulnerability in motor neurone disease: amyotrophic lateral sclerosis and spinal muscular atrophy","volume":"36","author":"Murray","year":"2010","journal-title":"Neuropathol. Appl. Neurobiol."},{"key":"10.1016\/j.scr.2015.07.005_bb0120","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1471-4159.2003.02009.x","article-title":"Inclusion formation in Huntington's disease R6\/2 mouse muscle cultures","volume":"87","author":"Orth","year":"2003","journal-title":"J. Neurochem."},{"key":"10.1016\/j.scr.2015.07.005_bb0125","doi-asserted-by":"crossref","first-page":"179","DOI":"10.3349\/ymj.2006.47.2.179","article-title":"A comparative study of magnetic-activated cell sorting, cytotoxicity and preplating for the purification of human myoblasts","volume":"47","author":"Park","year":"2006","journal-title":"Yonsei Med. J."},{"key":"10.1016\/j.scr.2015.07.005_bb0130","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.yexcr.2004.08.004","article-title":"Desmin: a major intermediate filament protein essential for the structural integrity and function of muscle","volume":"301","author":"Paulin","year":"2004","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.scr.2015.07.005_bb0135","doi-asserted-by":"crossref","first-page":"8275","DOI":"10.1073\/pnas.1322258111","article-title":"Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro","volume":"111","author":"Skoglund","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.scr.2015.07.005_bb0140","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1634\/stemcells.2008-1075","article-title":"Induced pluripotent stem cell generation using a single lentiviral stem cell cassette","volume":"27","author":"Sommer","year":"2009","journal-title":"Stem Cells"},{"key":"10.1016\/j.scr.2015.07.005_bb0145","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3892\/ijmm.2013.1555","article-title":"Evaluation of the effects of different culture media on the myogenic differentiation potential of adipose tissue- or bone marrow-derived human mesenchymal stem cells","volume":"33","author":"Stern-Straeter","year":"2014","journal-title":"Int. J. Mol. Med."},{"key":"10.1016\/j.scr.2015.07.005_bb0150","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/s12015-011-9329-4","article-title":"Developmental and functional nature of human iPSC derived motoneurons","volume":"9","author":"Stockmann","year":"2011","journal-title":"Stem Cell Rev."},{"key":"10.1016\/j.scr.2015.07.005_bb0155","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1007\/s00702-012-0910-z","article-title":"The dynactin p150 subunit: cell biology studies of sequence changes found in ALS\/MND and Parkinsonian syndromes","volume":"120","author":"Stockmann","year":"2013","journal-title":"J. Neural Transm."},{"key":"10.1016\/j.scr.2015.07.005_bb0160","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1371\/annotation\/63972dc9-3a31-43d0-ad52-bc46fd948c03","article-title":"Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi myopathy in vitro","volume":"8","author":"Tanaka","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.scr.2015.07.005_bb0165","doi-asserted-by":"crossref","first-page":"2284","DOI":"10.1093\/hmg\/ddq106","article-title":"Skeletal muscle-restricted expression of human SOD1 causes motor neuron degeneration in transgenic mice","volume":"19","author":"Wong","year":"2010","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.scr.2015.07.005_bb0170","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1369\/jhc.6R6995.2006","article-title":"The skeletal muscle satellite cell: the stem cell that came in from the cold","volume":"54","author":"Zammit","year":"2006","journal-title":"J. Histochem. Cytochem."}],"container-title":["Stem Cell Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1873506115001026?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1873506115001026?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T14:50:13Z","timestamp":1567003813000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1873506115001026"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["S1873506115001026"],"URL":"https:\/\/doi.org\/10.1016\/j.scr.2015.07.005","relation":{},"ISSN":["1873-5061"],"issn-type":[{"value":"1873-5061","type":"print"}],"subject":[],"published":{"date-parts":[[2015,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Formation and characterisation of neuromuscular junctions between hiPSC derived motoneurons and myotubes","name":"articletitle","label":"Article Title"},{"value":"Stem Cell Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.scr.2015.07.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}