{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T07:42:49Z","timestamp":1785224569884,"version":"3.55.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T00:00:00Z","timestamp":1629676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Saudi Journal of Biological Sciences"],"published-print":{"date-parts":[[2022,1]]},"DOI":"10.1016\/j.sjbs.2021.08.070","type":"journal-article","created":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T11:52:35Z","timestamp":1629978755000},"page":"204-210","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"title":["Epidemiological screening and serotyping analysis of dengue fever in the Southwestern region of Saudi Arabia"],"prefix":"10.1016","volume":"29","author":[{"given":"Alkhansa","family":"Alshabi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amani","family":"Marwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuzhath","family":"Fatima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aymen M.","family":"Madkhali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemah","family":"Alnagai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abrar","family":"Alhazmi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hesham M.","family":"Al-Mekhlafi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Abdulhaq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid Y.","family":"Ghailan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Sali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tareq","family":"Refaei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.sjbs.2021.08.070_b0005","first-page":"104","article-title":"Evaluation of radon related parameters in environmental samples from Jazan city, Saudi Arabia","volume":"11","author":"Abo-Elmagd","year":"2018","journal-title":"J. Radiat. Res. Appl. Sci."},{"key":"10.1016\/j.sjbs.2021.08.070_b0010","doi-asserted-by":"crossref","first-page":"194","DOI":"10.4103\/0972-9062.141759","article-title":"Comparison of NS1 antigen detection ELISA, real time RT-PCR and virus isolation for rapid diagnosis of dengue infection in acute phase","volume":"51","author":"Ahmed","year":"2014","journal-title":"J. Vector Borne Dis."},{"key":"10.1016\/j.sjbs.2021.08.070_b0015","doi-asserted-by":"crossref","first-page":"e0007144","DOI":"10.1371\/journal.pntd.0007144","article-title":"Assessment of the new World Health Organization's dengue classification for predicting severity of illness and level of healthcare required","volume":"13","author":"Ajlan","year":"2019","journal-title":"PLoS Negl.Trop. Dis."},{"issue":"2","key":"10.1016\/j.sjbs.2021.08.070_b0020","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.jiph.2015.05.006","article-title":"The epidemiology of Dengue fever in Saudi Arabia: A systematic review","volume":"9","author":"Alhaeli","year":"2016","journal-title":"J. Infection Public Health"},{"issue":"2","key":"10.1016\/j.sjbs.2021.08.070_b0025","doi-asserted-by":"crossref","first-page":"235","DOI":"10.21608\/jesp.2017.77745","article-title":"Serotypes of dengue viruses circulating in Jazan region, Saudi Arabia","volume":"47","author":"Alsheikh","year":"2017","journal-title":"J. Egypt. Soc. Parasitol."},{"key":"10.1016\/j.sjbs.2021.08.070_b0030","first-page":"1","article-title":"Environmental epidemiology and neurological manifestations of dengue serotypes with special inference on molecular trends, virus detection, and pathogenicity","author":"Anoopkumar","year":"2021","journal-title":"Environ. Dev. Sustainability"},{"issue":"2","key":"10.1016\/j.sjbs.2021.08.070_b0035","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.trstmh.2005.05.015","article-title":"The performance of RT-PCR compared with a rapid serological assay for acute dengue fever in a diagnostic laboratory","volume":"100","author":"Barkham","year":"2006","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"issue":"7446","key":"10.1016\/j.sjbs.2021.08.070_b0040","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"issue":"8","key":"10.1016\/j.sjbs.2021.08.070_b0045","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1002\/jmv.21203","article-title":"A simple one-step real-time RT-PCR for diagnosis of dengue virus infection","volume":"80","author":"dos Santos","year":"2008","journal-title":"J. Med. Virol."},{"issue":"4","key":"10.1016\/j.sjbs.2021.08.070_b0050","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1007\/s00580-013-1704-x","article-title":"Molecular and seroprevalence of imported dengue virus infection in Al-Madinah, Saudi Arabia","volume":"23","author":"El-Badry","year":"2014","journal-title":"Comp. Clin. Pathol."},{"key":"10.1016\/j.sjbs.2021.08.070_b0055","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12889-020-08656-y","article-title":"Recent transmission of dengue virus and associated risk Facors among residents of Kassala state, eastern Sudan","volume":"20","author":"Eldigail","year":"2020","journal-title":"BMC Public Health"},{"key":"10.1016\/j.sjbs.2021.08.070_b0060","doi-asserted-by":"crossref","first-page":"764","DOI":"10.4269\/ajtmh.2001.65.764","article-title":"Virologic and serologic surveillance for dengue fever in Jeddah, Saudi Arabia, 1994\u20131999","volume":"65","author":"Fakeeh","year":"2001","journal-title":"Am. J. Tropical Med. Hygiene"},{"key":"10.1016\/j.sjbs.2021.08.070_b0065","first-page":"85","article-title":"Prevalence of dengue among clinically suspected febrile episodes at a teaching hospital in North India","volume":"3","author":"Garg","year":"2011","journal-title":"J. Infect. Dis. Immunity"},{"issue":"4","key":"10.1016\/j.sjbs.2021.08.070_b0070","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1111\/j.1469-0691.2006.01353.x","article-title":"Evaluation of the use of RT-PCR for the early diagnosis of dengue fever","volume":"12","author":"Grobusch","year":"2006","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.sjbs.2021.08.070_b0075","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2017.00317","article-title":"Global epidemiology of dengue outbreaks in 1990\u20132015: a systematic review and meta-analysis","volume":"7","author":"Guo","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"1","key":"10.1016\/j.sjbs.2021.08.070_b0080","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/1743-422X-6-10","article-title":"Development of real time PCR for detection and quantitation of dengue viruses","volume":"6","author":"Gurukumar","year":"2009","journal-title":"Virol. J."},{"issue":"sup1","key":"10.1016\/j.sjbs.2021.08.070_b0085","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1179\/2046904712Z.00000000052","article-title":"The revised WHO dengue case classification: does the system need to be modified?","volume":"32","author":"Hadinegoro","year":"2012","journal-title":"Paediatrics int. Child Health"},{"issue":"9599","key":"10.1016\/j.sjbs.2021.08.070_b0090","doi-asserted-by":"crossref","first-page":"1644","DOI":"10.1016\/S0140-6736(07)61687-0","article-title":"Dengue","volume":"370","author":"Halstead","year":"2007","journal-title":"Lancet"},{"issue":"6","key":"10.1016\/j.sjbs.2021.08.070_b0095","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.1128\/JCM.06557-11","article-title":"Detection of dengue virus genome in urine by real-time reverse transcriptase PCR: a laboratory diagnostic method useful after disappearance of the genome in serum","volume":"50","author":"Hirayama","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.sjbs.2021.08.070_b0100","doi-asserted-by":"crossref","first-page":"184","DOI":"10.4103\/1658-600X.159910","article-title":"Relationship between PTEN and gestational diabetes in Asian Indians womens","volume":"3","author":"Khan","year":"2015","journal-title":"J. Health Specialties"},{"issue":"1","key":"10.1016\/j.sjbs.2021.08.070_b0105","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/j.dsx.2018.11.035","article-title":"Genetic confirmation of T2DM meta-analysis variants studied in gestational diabetes mellitus in an Indian population","volume":"13","author":"Khan","year":"2019","journal-title":"Diabetes Metab. Syndr."},{"issue":"9","key":"10.1016\/j.sjbs.2021.08.070_b0110","doi-asserted-by":"crossref","DOI":"10.1002\/jcla.23371","article-title":"Prevalence of dengue virus serotypes in the 2017 outbreak in Peshawar, KP, Pakistan","volume":"34","author":"Khan","year":"2020","journal-title":"J. Clin. Lab. Analysis"},{"key":"10.1016\/j.sjbs.2021.08.070_b0115","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.3389\/fmicb.2020.01371","article-title":"Application of next-generation sequencing to reveal how evolutionary dynamics of viral population shape dengue epidemiology","volume":"11","author":"Ko","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.sjbs.2021.08.070_b0120","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2020.102790","article-title":"A (class-) switch in the antibody response may distinguish primary from secondary dengue virus infection","volume":"56","author":"Levoir","year":"2020","journal-title":"EBioMedicine"},{"key":"10.1016\/j.sjbs.2021.08.070_b0125","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.3389\/fpubh.2020.603872","article-title":"Determination of factors affecting dengue occurrence in representative areas of China: a principal component regression analysis","volume":"8","author":"Liu","year":"2021","journal-title":"Front. Public Health"},{"issue":"1","key":"10.1016\/j.sjbs.2021.08.070_b0130","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1002\/jmv.23981","article-title":"Molecular characterization of dengue viruses circulating during 2009\u20132012 in Uttar Pradesh, India","volume":"87","author":"Mishra","year":"2015","journal-title":"J. Med. Virol."},{"issue":"7","key":"10.1016\/j.sjbs.2021.08.070_b0135","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1016\/j.trstmh.2007.02.007","article-title":"Confirmation of dengue virus infection by detection of dengue virus type 1 genome in urine and saliva but not in plasma","volume":"101","author":"Mizuno","year":"2007","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"issue":"8","key":"10.1016\/j.sjbs.2021.08.070_b0140","doi-asserted-by":"crossref","first-page":"e1065","DOI":"10.1016\/S2214-109X(19)30250-5","article-title":"Burden of dengue infection in India, 2017: a cross-sectional population based serosurvey","volume":"7","author":"Murhekar","year":"2019","journal-title":"Lancet Global Health"},{"issue":"6","key":"10.1016\/j.sjbs.2021.08.070_b0145","doi-asserted-by":"crossref","first-page":"593","DOI":"10.17511\/ijmrr.2015.i6.112","article-title":"Seroprevalence of Dengue infection in clinically suspected cases of dengue at tertiary care hospital in central India","volume":"3","author":"Nikam","year":"2015","journal-title":"Int. J. Med. Res. Rev."},{"key":"10.1016\/j.sjbs.2021.08.070_b0150","unstructured":"Organization, W.H. (2011). Comprehensive guideline for prevention and control of dengue and dengue haemorrhagic fever."},{"key":"10.1016\/j.sjbs.2021.08.070_b0155","first-page":"862","article-title":"Trend of dengue virus infection at Lucknow, north India (2008\u20132010): a hospital based study","volume":"136","author":"Pandey","year":"2012","journal-title":"Indian J. Med. Res."},{"issue":"1","key":"10.1016\/j.sjbs.2021.08.070_b0160","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/s10712-020-09623-3","article-title":"Use of Geophysical and Radar Interferometric Techniques to Monitor Land Deformation Associated with the Jazan Salt Diapir, Jazan city, Saudi Arabia","volume":"42","author":"Pankratz","year":"2021","journal-title":"Surv. Geophys."},{"key":"10.1016\/j.sjbs.2021.08.070_b0165","first-page":"1132","article-title":"Infections caused by arthropod-and rodent-borne viruses","author":"Peters","year":"1998","journal-title":"Harrisons Principles Internal Med."},{"issue":"1","key":"10.1016\/j.sjbs.2021.08.070_b0170","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1743-422X-7-22","article-title":"Detection of dengue virus in saliva and urine by real time RT-PCR","volume":"7","author":"Poloni","year":"2010","journal-title":"Virol. J."},{"key":"10.1016\/j.sjbs.2021.08.070_b0175","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12879-020-4902-6","article-title":"The time series seasonal patterns of dengue fever and associated weather variables in Bangkok (2003\u20132017)","volume":"20","author":"Polwiang","year":"2020","journal-title":"BMC Infect. Dis."},{"issue":"8","key":"10.1016\/j.sjbs.2021.08.070_b0180","doi-asserted-by":"crossref","first-page":"e103815","DOI":"10.1371\/journal.pone.0103815","article-title":"Performance of Simplexa dengue molecular assay compared to conventional and SYBR green RT-PCR for detection of dengue infection in Indonesia","volume":"9","author":"Sasmono","year":"2014","journal-title":"PLoS ONE"},{"key":"10.1016\/j.sjbs.2021.08.070_b0185","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2020.00352","article-title":"Dengue virus serotype 2 and its non-structural proteins 2A and 2B activate NLRP3 inflammasome","volume":"11","author":"Shrivastava","year":"2020","journal-title":"Front. Immunol."},{"issue":"7","key":"10.1016\/j.sjbs.2021.08.070_b0190","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1007\/s10096-015-2359-0","article-title":"Recovery of dengue virus from urine samples by real-time RT-PCR","volume":"34","author":"Van den Bossche","year":"2015","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.sjbs.2021.08.070_b0195","doi-asserted-by":"crossref","first-page":"93","DOI":"10.3389\/fimmu.2014.00093","article-title":"T-cell immunity to infection with dengue virus in humans","volume":"5","author":"Weiskopf","year":"2014","journal-title":"Front. Immunol."},{"issue":"8","key":"10.1016\/j.sjbs.2021.08.070_b0200","doi-asserted-by":"crossref","first-page":"e988","DOI":"10.1016\/S2214-109X(19)30249-9","article-title":"Estimating the dengue burden in India","volume":"7","author":"Wilder-Smith","year":"2019","journal-title":"Lancet Global Health"}],"container-title":["Saudi Journal of Biological Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1319562X21007580?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1319562X21007580?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:19:29Z","timestamp":1761153569000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1319562X21007580"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["S1319562X21007580"],"URL":"https:\/\/doi.org\/10.1016\/j.sjbs.2021.08.070","relation":{},"ISSN":["1319-562X"],"issn-type":[{"value":"1319-562X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Epidemiological screening and serotyping analysis of dengue fever in the Southwestern region of Saudi Arabia","name":"articletitle","label":"Article Title"},{"value":"Saudi Journal of Biological Sciences","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.sjbs.2021.08.070","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University.","name":"copyright","label":"Copyright"}]}}