{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T16:00:10Z","timestamp":1772121610619,"version":"3.50.1"},"reference-count":60,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,10,5]],"date-time":"2021-10-05T00:00:00Z","timestamp":1633392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008893","name":"University of Iowa","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008893","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004339","name":"Sanofi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004339","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005725","name":"CHDI Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006961","name":"California Institute of Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006961","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["slas-discovery.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["SLAS Discovery"],"published-print":{"date-parts":[[2022,6]]},"DOI":"10.1016\/j.slasd.2021.10.005","type":"journal-article","created":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T00:26:52Z","timestamp":1643070412000},"page":"219-228","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["Time-resolved FRET screening identifies small molecular modifiers of mutant Huntingtin conformational inflexibility in patient-derived cells"],"prefix":"10.1016","volume":"27","author":[{"given":"Johannes H.","family":"Wilbertz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Frappier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Muller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Gratzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter","family":"Englaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa M.","family":"Stanek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Calamini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.slasd.2021.10.005_bib0001","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1002\/mds.25075","article-title":"The Incidence and Prevalence of Huntington's Disease: A Systematic Review and Meta-Analysis","volume":"27","author":"Pringsheim","year":"2012","journal-title":"Mov Disord"},{"key":"10.1016\/j.slasd.2021.10.005_bib0002","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1111\/ene.13413","article-title":"Huntington's Disease: A Clinical Review","volume":"25","author":"McColgan","year":"2018","journal-title":"Eur J Neurol"},{"key":"10.1016\/j.slasd.2021.10.005_bib0003","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1042\/BJ20071619","article-title":"Huntington's Disease: From Pathology and Genetics to Potential Therapies","volume":"412","author":"Imarisio","year":"2008","journal-title":"Biochem J"},{"key":"10.1016\/j.slasd.2021.10.005_bib0004","doi-asserted-by":"crossref","first-page":"e82","DOI":"10.1371\/journal.pgen.0030082","article-title":"Huntingtin Interacting Proteins Are Genetic Modifiers of Neurodegeneration","volume":"3","author":"Kaltenbach","year":"2007","journal-title":"PLOS Genet"},{"key":"10.1016\/j.slasd.2021.10.005_bib0005","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.tig.2004.01.008","article-title":"Huntingtin-Protein Interactions and the Pathogenesis of Huntington's Disease","volume":"20","author":"Li","year":"2004","journal-title":"Trends Genet. TIG"},{"key":"10.1016\/j.slasd.2021.10.005_bib0006","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/s41586-019-1722-1","article-title":"Allele-Selective Lowering of Mutant HTT Protein by HTT\u2013LC3 Linker Compounds","volume":"575","author":"Li","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.slasd.2021.10.005_bib0007","doi-asserted-by":"crossref","first-page":"2307","DOI":"10.1056\/NEJMoa1900907","article-title":"Targeting Huntingtin Expression in Patients with Huntington's Disease","volume":"380","author":"Tabrizi","year":"2019","journal-title":"N Engl J Med"},{"key":"10.1016\/j.slasd.2021.10.005_bib0008","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.1093\/hmg\/10.22.2515","article-title":"The HD Mutation Causes Progressive Lethal Neurological Disease in Mice Expressing Reduced Levels of Huntingtin","volume":"10","author":"Auerbach","year":"2001","journal-title":"Hum Mol Genet"},{"key":"10.1016\/j.slasd.2021.10.005_bib0009","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1016\/j.neuron.2016.02.003","article-title":"The Biology of Huntingtin","volume":"89","author":"Saudou","year":"2016","journal-title":"Neuron"},{"key":"10.1016\/j.slasd.2021.10.005_bib0010","doi-asserted-by":"crossref","first-page":"3359","DOI":"10.1073\/pnas.1524575113","article-title":"Ablation of Huntingtin in Adult Neurons Is Nondeleterious but Its Depletion in Young Mice Causes Acute Pancreatitis","volume":"113","author":"Wang","year":"2016","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.slasd.2021.10.005_bib0011","doi-asserted-by":"crossref","first-page":"5736","DOI":"10.1073\/pnas.1603871113","article-title":"Selective Expression of Mutant Huntingtin during Development Recapitulates Characteristic Features of Huntington's Disease","volume":"113","author":"Molero","year":"2016","journal-title":"Proc Natl Acad Sci"},{"key":"10.1016\/j.slasd.2021.10.005_bib0012","doi-asserted-by":"crossref","first-page":"2582","DOI":"10.1038\/s41467-020-16318-1","article-title":"Truncation of Mutant Huntingtin in Knock-in Mice Demonstrates Exon1 Huntingtin Is a Key Pathogenic Form","volume":"11","author":"Yang","year":"2020","journal-title":"Nat Commun"},{"key":"10.1016\/j.slasd.2021.10.005_bib0013","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.celrep.2019.12.069","article-title":"Striatal Projection Neurons Require Huntingtin for Synaptic Connectivity and Survival","volume":"30","author":"Burrus","year":"2020","journal-title":"Cell Rep"},{"key":"10.1016\/j.slasd.2021.10.005_bib0014","doi-asserted-by":"crossref","DOI":"10.1093\/hmg\/ddaa283","article-title":"Mutations Causing Lopes-Maciel-Rodan Syndrome Are Huntingtin Hypomorphs","author":"Jung","year":"2021","journal-title":"Hum Mol Genet"},{"key":"10.1016\/j.slasd.2021.10.005_bib0015","doi-asserted-by":"crossref","first-page":"14610","DOI":"10.1073\/pnas.1301342110","article-title":"Polyglutamine Domain Flexibility Mediates the Proximity between Flanking Sequences in Huntingtin","volume":"110","author":"Caron","year":"2013","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.slasd.2021.10.005_bib0016","doi-asserted-by":"crossref","first-page":"8820","DOI":"10.1021\/jacs.7b00838","article-title":"Emerging \u03b2-Sheet Rich Conformations in Super-Compact Huntingtin Exon-1 Mutant Structures","volume":"139","author":"Kang","year":"2017","journal-title":"J Am Chem Soc"},{"key":"10.1016\/j.slasd.2021.10.005_bib0017","doi-asserted-by":"crossref","first-page":"19613","DOI":"10.1074\/jbc.RA118.004808","article-title":"The Folding Equilibrium of Huntingtin Exon 1 Monomer Depends on Its Polyglutamine Tract","volume":"293","author":"Bravo-Arredondo","year":"2018","journal-title":"J Biol Chem"},{"key":"10.1016\/j.slasd.2021.10.005_bib0018","doi-asserted-by":"crossref","first-page":"5070","DOI":"10.1038\/s41598-017-05336-7","article-title":"Polyglutamine Expansion Affects Huntingtin Conformation in Multiple Huntington's Disease Models","volume":"7","author":"Daldin","year":"2017","journal-title":"Sci Rep"},{"key":"10.1016\/j.slasd.2021.10.005_bib0019","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0112262","article-title":"Polyglutamine- and Temperature-Dependent Conformational Rigidity in Mutant Huntingtin Revealed by Immunoassays and Circular Dichroism Spectroscopy","volume":"9","author":"Fodale","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.slasd.2021.10.005_bib0020","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/srep05601","article-title":"TR-FRET Assays of Huntingtin Protein Fragments Reveal Temperature and PolyQ Length-Dependent Conformational Changes","volume":"4","author":"Cui","year":"2014","journal-title":"Sci Rep"},{"key":"10.1016\/j.slasd.2021.10.005_bib0021","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1016\/j.jmb.2009.12.017","article-title":"Modulation of Polyglutamine Conformations and Dimer Formation by the N-Terminus of Huntingtin","volume":"396","author":"Williamson","year":"2010","journal-title":"J Mol Biol"},{"key":"10.1016\/j.slasd.2021.10.005_bib0022","article-title":"Towards Deciphering the Nt17 Code: How the Sequence and Conformation of the First 17 Amino Acids in Huntingtin Regulate the Aggregation, Cellular Properties and Neurotoxicity of Mutant Httex1","author":"Vieweg","year":"2021","journal-title":"bioRxiv"},{"key":"10.1016\/j.slasd.2021.10.005_bib0023","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1016\/j.neuron.2009.11.020","article-title":"Serines 13 and 16 Are Critical Determinants of Full-Length Human Mutant Huntingtin Induced Disease Pathogenesis in HD Mice","volume":"64","author":"Gu","year":"2009","journal-title":"Neuron"},{"key":"10.1016\/j.slasd.2021.10.005_bib0024","doi-asserted-by":"crossref","first-page":"19238","DOI":"10.1074\/jbc.M117.782300","article-title":"Post-Translational Modifications Clustering within Proteolytic Domains Decrease Mutant Huntingtin Toxicity","volume":"292","author":"Arbez","year":"2017","journal-title":"J Biol Chem"},{"key":"10.1016\/j.slasd.2021.10.005_bib0025","doi-asserted-by":"crossref","first-page":"E10809","DOI":"10.1073\/pnas.1705372114","article-title":"Phosphorylation of Huntingtin at Residue T3 Is Decreased in Huntington's Disease and Modulates Mutant Huntingtin Protein Conformation","volume":"114","author":"Cariulo","year":"2017","journal-title":"Proc Natl Acad Sci"},{"key":"10.1016\/j.slasd.2021.10.005_bib0026","doi-asserted-by":"crossref","first-page":"18540","DOI":"10.1074\/jbc.RA118.004621","article-title":"N-Terminal Huntingtin (Htt) Phosphorylation Is a Molecular Switch Regulating Htt Aggregation, Helical Conformation, Internalization, and Nuclear Targeting","volume":"293","author":"DeGuire","year":"2018","journal-title":"J Biol Chem"},{"key":"10.1016\/j.slasd.2021.10.005_bib0027","doi-asserted-by":"crossref","first-page":"3528","DOI":"10.1073\/pnas.1114502109","article-title":"Ganglioside GM1 Induces Phosphorylation of Mutant Huntingtin and Restores Normal Motor Behavior in Huntington Disease Mice","volume":"109","author":"Pardo","year":"2012","journal-title":"Proc Natl Acad Sci"},{"key":"10.1016\/j.slasd.2021.10.005_bib0028","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/nchembio.582","article-title":"Kinase Inhibitors Modulate Huntingtin Cell Localization and Toxicity","volume":"7","author":"Atwal","year":"2011","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.slasd.2021.10.005_bib0029","doi-asserted-by":"crossref","first-page":"E7081","DOI":"10.1073\/pnas.1801772115","article-title":"N6-Furfuryladenine Is Protective in Huntington's Disease Models by Signaling Huntingtin Phosphorylation","volume":"115","author":"Bowie","year":"2018","journal-title":"Proc Natl Acad Sci"},{"key":"10.1016\/j.slasd.2021.10.005_bib0030","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/j.cell.2019.06.036","article-title":"CAG Repeat Not Polyglutamine Length Determines Timing of Huntington's Disease Onset","volume":"178","author":"Lee","year":"2019","journal-title":"Cell"},{"key":"10.1016\/j.slasd.2021.10.005_bib0031","series-title":"GeneReviews\u00ae","article-title":"Huntington Disease","author":"Caron","year":"1993"},{"key":"10.1016\/j.slasd.2021.10.005_bib0032","doi-asserted-by":"crossref","first-page":"3498","DOI":"10.1073\/pnas.0308679101","article-title":"Venezuelan Kindreds Reveal That Genetic and Environmental Factors Modulate Huntington's Disease Age of Onset","volume":"101","author":"Wexler","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.slasd.2021.10.005_bib0033","doi-asserted-by":"crossref","first-page":"e430","DOI":"10.1212\/NXG.0000000000000430","article-title":"Genotyping Single Nucleotide Polymorphisms for Allele-Selective Therapy in Huntington Disease","volume":"6","author":"Claassen","year":"2020","journal-title":"Neurol Genet"},{"key":"10.1016\/j.slasd.2021.10.005_bib0034","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1007\/s10072-019-04233-3","article-title":"Frequency and Distribution of PolyQ Disease Intermediate-Length Repeat Alleles in Healthy Italian Population","volume":"41","author":"Mongelli","year":"2020","journal-title":"Neurol Sci"},{"key":"10.1016\/j.slasd.2021.10.005_bib0035","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S0361-9230(01)00599-8","article-title":"New Anti-Huntingtin Monoclonal Antibodies: Implications for Huntingtin Conformation and Its Binding Proteins","volume":"56","author":"Ko","year":"2001","journal-title":"Brain Res Bull"},{"key":"10.1016\/j.slasd.2021.10.005_bib0036","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An Open-Source Platform for Biological-Image Analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat Methods"},{"key":"10.1016\/j.slasd.2021.10.005_bib0037","series-title":"Studies in Classification, Data Analysis, and Knowledge Organization","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/978-3-540-78246-9_38","article-title":"KNIME: The Konstanz Information Miner","author":"Berthold","year":"2008"},{"key":"10.1016\/j.slasd.2021.10.005_bib0038","first-page":"2020","volume":"11","author":"Waskom","year":"2020"},{"key":"10.1016\/j.slasd.2021.10.005_bib0039","doi-asserted-by":"crossref","first-page":"6063","DOI":"10.1074\/jbc.M112.413575","article-title":"An N-Terminal Nuclear Export Signal Regulates Trafficking and Aggregation of Huntingtin (Htt) Protein Exon 1","volume":"288","author":"Zheng","year":"2013","journal-title":"J Biol Chem"},{"key":"10.1016\/j.slasd.2021.10.005_bib0040","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1083\/jcb.200909067","article-title":"IKK Phosphorylates Huntingtin and Targets It for Degradation by the Proteasome and Lysosome","volume":"187","author":"Thompson","year":"2009","journal-title":"J Cell Biol"},{"key":"10.1016\/j.slasd.2021.10.005_bib0041","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jmb.2012.09.011","article-title":"Serine Phosphorylation Suppresses Huntingtin Amyloid Accumulation by Altering Protein Aggregation Properties","volume":"424","author":"Mishra","year":"2012","journal-title":"J Mol Biol"},{"key":"10.1016\/j.slasd.2021.10.005_bib0042","series-title":"Eli Lilly & Company and the National Center for Advancing Translational Sciences","article-title":"Imaging Platform, Broad Institute of MIT and Harvard. Advanced Assay Development Guidelines for Image-Based High Content Screening and Analysis","author":"Bray","year":"2004"},{"key":"10.1016\/j.slasd.2021.10.005_bib0043","doi-asserted-by":"crossref","first-page":"95","DOI":"10.3389\/fmolb.2019.00095","article-title":"The Role of Post-Translational Modifications on the Energy Landscape of Huntingtin N-Terminus","volume":"6","author":"Yalinca","year":"2019","journal-title":"Front Mol Biosci"},{"key":"10.1016\/j.slasd.2021.10.005_bib0044","doi-asserted-by":"crossref","first-page":"5202","DOI":"10.1002\/anie.201611750","article-title":"Mutant Exon1 Huntingtin Aggregation Is Regulated by T3 Phosphorylation-Induced Structural Changes and Crosstalk between T3 Phosphorylation and Acetylation at K6","volume":"56","author":"Chiki","year":"2017","journal-title":"Angew Chem Int Ed Engl"},{"key":"10.1016\/j.slasd.2021.10.005_bib0045","doi-asserted-by":"crossref","first-page":"18540","DOI":"10.1074\/jbc.RA118.004621","article-title":"N-Terminal Huntingtin (Htt) Phosphorylation Is a Molecular Switch Regulating Htt Aggregation, Helical Conformation, Internalization, and Nuclear Targeting","volume":"293","author":"DeGuire","year":"2018","journal-title":"J Biol Chem"},{"key":"10.1016\/j.slasd.2021.10.005_bib0046","doi-asserted-by":"crossref","first-page":"7615","DOI":"10.1038\/s41598-019-44151-0","article-title":"Molecular Mechanisms of Heterogeneous Oligomerization of Huntingtin Proteins","volume":"9","author":"Bonfanti","year":"2019","journal-title":"Sci Rep"},{"key":"10.1016\/j.slasd.2021.10.005_bib0047","doi-asserted-by":"crossref","first-page":"3763","DOI":"10.1093\/hmg\/ddx260","article-title":"Protein Phosphatase 1 Regulates Huntingtin Exon 1 Aggregation and Toxicity","volume":"26","author":"Branco-Santos","year":"2017","journal-title":"Hum Mol Genet"},{"key":"10.1016\/j.slasd.2021.10.005_bib0048","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.bpj.2016.06.018","article-title":"Acetylation within the First 17 Residues of Huntingtin Exon 1 Alters Aggregation and Lipid Binding","volume":"111","author":"Chaibva","year":"2016","journal-title":"Biophys J"},{"key":"10.1016\/j.slasd.2021.10.005_bib0049","doi-asserted-by":"crossref","first-page":"5202","DOI":"10.1002\/anie.201611750","article-title":"Mutant Exon1 Huntingtin Aggregation Is Regulated by T3 Phosphorylation-Induced Structural Changes and Crosstalk between T3 Phosphorylation and Acetylation at K6","volume":"56","author":"Chiki","year":"2017","journal-title":"Angew Chem, Int Ed"},{"key":"10.1016\/j.slasd.2021.10.005_bib0050","doi-asserted-by":"crossref","first-page":"e88284","DOI":"10.1371\/journal.pone.0088284","article-title":"Phosphorylation of Mutant Huntingtin at Serine 116 Modulates Neuronal Toxicity","volume":"9","author":"Watkin","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.slasd.2021.10.005_bib0051","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1083\/jcb.200412071","article-title":"Cdk5 Phosphorylation of Huntingtin Reduces Its Cleavage by Caspases","volume":"169","author":"Luo","year":"2005","journal-title":"J Cell Biol"},{"key":"10.1016\/j.slasd.2021.10.005_bib0052","doi-asserted-by":"crossref","first-page":"23686","DOI":"10.1074\/jbc.M513507200","article-title":"Huntingtin Phosphorylation Sites Mapped by Mass Spectrometry. Modulation of Cleavage and Toxicity","volume":"281","author":"Schilling","year":"2006","journal-title":"J Biol Chem"},{"key":"10.1016\/j.slasd.2021.10.005_bib0053","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.chembiol.2011.12.020","article-title":"TR-FRET-Based Duplex Immunoassay Reveals an Inverse Correlation of Soluble and Aggregated Mutant Huntingtin in Huntington's Disease","volume":"19","author":"Baldo","year":"2012","journal-title":"Chem Biol"},{"key":"10.1016\/j.slasd.2021.10.005_bib0054","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0213521","article-title":"Meso Scale Discovery-Based Assays for the Detection of Aggregated Huntingtin","volume":"14","author":"Reindl","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.slasd.2021.10.005_bib0055","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1038\/nrg2828","article-title":"Mechanisms of Trinucleotide Repeat Instability during Human Development","volume":"11","author":"McMurray","year":"2010","journal-title":"Nat Rev Genet"},{"key":"10.1016\/j.slasd.2021.10.005_bib0056","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1017\/S031716710000514X","article-title":"Canadian Association of Neurosciences Review: Polyglutamine Expansion Neurodegenerative Diseases","volume":"33","author":"Truant","year":"2006","journal-title":"Can J Neurol Sci"},{"key":"10.1016\/j.slasd.2021.10.005_bib0057","first-page":"475","article-title":"Big Impact: Posttranslational Modifications and Function of Huntingtin in Huntington Disease","volume":"17","author":"Ehrnhoefer","year":"2011","journal-title":"Neurosci Rev J Bringing Neurobiol Neurol Psychiatry"},{"key":"10.1016\/j.slasd.2021.10.005_bib0058","doi-asserted-by":"crossref","first-page":"e62043","DOI":"10.1371\/journal.pone.0062043","article-title":"Cerebellar Soluble Mutant Ataxin-3 Level Decreases during Disease Progression in Spinocerebellar Ataxia Type 3 Mice","volume":"8","author":"Nguyen","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.slasd.2021.10.005_bib0059","doi-asserted-by":"crossref","first-page":"e11803","DOI":"10.15252\/emmm.201911803","article-title":"Neurofilaments in Spinocerebellar Ataxia Type 3: Blood Biomarkers at the Preataxic and Ataxic Stage in Humans and Mice","volume":"12","author":"Wilke","year":"2020","journal-title":"EMBO Mol Med"},{"key":"10.1016\/j.slasd.2021.10.005_bib0060","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1007\/s00415-020-10274-y","article-title":"PolyQ-Expanded Ataxin-3 Protein Levels in Peripheral Blood Mononuclear Cells Correlate with Clinical Parameters in SCA3: A Pilot Study","volume":"268","author":"Gonsior","year":"2020","journal-title":"J Neurol"}],"container-title":["SLAS Discovery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555221000058?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555221000058?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T11:55:16Z","timestamp":1762862116000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2472555221000058"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6]]},"references-count":60,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["S2472555221000058"],"URL":"https:\/\/doi.org\/10.1016\/j.slasd.2021.10.005","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.01.18.427095","asserted-by":"object"}]},"ISSN":["2472-5552"],"issn-type":[{"value":"2472-5552","type":"print"}],"subject":[],"published":{"date-parts":[[2022,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Time-resolved FRET screening identifies small molecular modifiers of mutant Huntingtin conformational inflexibility in patient-derived cells","name":"articletitle","label":"Article Title"},{"value":"SLAS Discovery","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.slasd.2021.10.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Authors. Published by Elsevier Inc. on behalf of Society for Laboratory Automation and Screening.","name":"copyright","label":"Copyright"}]}}