{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T08:41:58Z","timestamp":1777452118145,"version":"3.51.4"},"reference-count":35,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T00:00:00Z","timestamp":1678665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["1U54AG065181"],"award-info":[{"award-number":["1U54AG065181"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["slas-discovery.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["SLAS Discovery"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1016\/j.slasd.2023.03.003","type":"journal-article","created":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T22:20:30Z","timestamp":1679005230000},"page":"170-179","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["A novel fluorogenic reporter substrate for 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (PLC\u03b32): Application to high-throughput screening for activators to treat Alzheimer's disease"],"prefix":"10.1016","volume":"28","author":[{"given":"Ramya","family":"Visvanathan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadanobu","family":"Utsuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel E.","family":"Beck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0619-8743","authenticated-orcid":false,"given":"Emma","family":"Lendy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuai-lin","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinghui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirk W.","family":"Hering","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Mesecar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong-Yin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0619-2616","authenticated-orcid":false,"given":"Karson S.","family":"Putt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.slasd.2023.03.003_bib0001","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1038\/ng.3916","article-title":"Rare coding variants in PLCG2, ABI3, and TREM2 implicate microglial-mediated innate immunity in Alzheimer's disease","volume":"49","author":"Sims","year":"2017","journal-title":"Nat Genet"},{"key":"10.1016\/j.slasd.2023.03.003_bib0002","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/s41398-018-0150-6","article-title":"GWAS on family history of Alzheimer's disease","volume":"8","author":"Marioni","year":"2018","journal-title":"Transl Psychiatry"},{"key":"10.1016\/j.slasd.2023.03.003_bib0003","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1038\/s41588-022-01024-z","article-title":"New insights into the genetic etiology of Alzheimer's disease and related dementias","volume":"54","author":"Bellenguez","year":"2022","journal-title":"Nat Genet"},{"key":"10.1016\/j.slasd.2023.03.003_bib0004","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/s13024-017-0184-x","article-title":"Late onset Alzheimer's disease genetics implicates microglial pathways in disease risk","volume":"12","author":"Efthymiou","year":"2017","journal-title":"Mol Neurodegener"},{"key":"10.1016\/j.slasd.2023.03.003_bib0005","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s13195-019-0469-0","article-title":"Alzheimer's disease phospholipase C-gamma-2 (PLCG2) protective variant is a functional hypermorph","volume":"11","author":"Magno","year":"2019","journal-title":"Alzheimer's Res Ther"},{"key":"10.1016\/j.slasd.2023.03.003_bib0006","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/s13024-018-0289-x","article-title":"ABI3 and PLCG2 missense variants as risk factors for neurodegenerative diseases in Caucasians and African Americans","volume":"13","author":"Conway","year":"2018","journal-title":"Mol Neurodegener"},{"key":"10.1016\/j.slasd.2023.03.003_bib0007","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s00401-019-02026-8","article-title":"A nonsynonymous mutation in PLCG2 reduces the risk of Alzheimer's disease, dementia with Lewy bodies and frontotemporal dementia, and increases the likelihood of longevity","volume":"138","author":"van der Lee","year":"2019","journal-title":"Acta Neuropathol"},{"key":"10.1016\/j.slasd.2023.03.003_bib0008","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/s41398-019-0394-9","article-title":"Transethnic meta-analysis of rare coding variants in PLCG2, ABI3, and TREM2 supports their general contribution to Alzheimer's disease","volume":"9","author":"Dalmasso","year":"2019","journal-title":"Transl Psychiatry"},{"key":"10.1016\/j.slasd.2023.03.003_bib0009","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1007\/s00401-020-02138-6","article-title":"PLCG2 protective variant p.P522R modulates tau pathology and disease progression in patients with mild cognitive impairment","volume":"139","author":"Kleineidam","year":"2020","journal-title":"Acta Neuropathol"},{"key":"10.1016\/j.slasd.2023.03.003_bib0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2021.100905","article-title":"The role of PLC\u03b32 in immunological disorders, cancer, and neurodegeneration","volume":"297","author":"Jackson","year":"2021","journal-title":"J Biol Chem"},{"key":"10.1016\/j.slasd.2023.03.003_bib0011","doi-asserted-by":"crossref","first-page":"4369","DOI":"10.3390\/jcm11154369","article-title":"Variant in the PLCG2 gene may cause a phenotypic overlap of APLAID\/PLAID: case series and literature review","volume":"11","author":"Welzel","year":"2022","journal-title":"J Clinic Med"},{"key":"10.1016\/j.slasd.2023.03.003_bib0012","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.ajhg.2012.08.006","article-title":"A hypermorphic missense mutation in PLCG2, encoding phospholipase C\u03b32, causes a dominantly inherited autoinflammatory disease with immunodeficiency","volume":"91","author":"Zhou","year":"2012","journal-title":"Am J Hum Genet"},{"key":"10.1016\/j.slasd.2023.03.003_bib0013","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1111\/jdv.15918","article-title":"Expanding the clinical features of autoinflammation and phospholipase C\u03b32-associated antibody deficiency and immune dysregulation by description of a novel patient","volume":"33","author":"Mor\u00e1n-Villase\u00f1or","year":"2019","journal-title":"J Eur Acad Dermatol Venereol"},{"key":"10.1016\/j.slasd.2023.03.003_bib0014","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/s10875-019-00731-3","article-title":"A germline mutation in the C2 domain of PLC\u03b32 associated with gain-of-function expands the phenotype for PLCG2-related diseases","volume":"40","author":"Novice","year":"2020","journal-title":"J Clin Immunol"},{"key":"10.1016\/j.slasd.2023.03.003_bib0015","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1007\/s10875-020-00794-7","article-title":"Severe autoinflammatory manifestations and antibody deficiency due to novel hypermorphic PLCG2 mutations","volume":"40","author":"Mart\u00edn-Nalda","year":"2020","journal-title":"J Clin Immunol"},{"key":"10.1016\/j.slasd.2023.03.003_bib0016","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2018.02863","article-title":"Novel PLCG2 mutation in a patient with APLAID and cutis laxa","volume":"9","author":"Neves","year":"2018","journal-title":"Front Immunol"},{"key":"10.1016\/j.slasd.2023.03.003_bib0017","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1002\/art.30280","article-title":"A novel N-ethyl-N-nitrosourea-induced mutation in phospholipase C\u03b32 causes inflammatory arthritis, metabolic defects, and male infertility in vitro in a murine model","volume":"63","author":"Abe","year":"2011","journal-title":"Arthrit Rheumat"},{"key":"10.1016\/j.slasd.2023.03.003_bib0018","doi-asserted-by":"crossref","first-page":"22136","DOI":"10.1074\/jbc.M116.746842","article-title":"The phospholipase C\u03b32 mutants R665W and L845F identified in ibrutinib-resistant chronic lymphocytic leukemia patients are hypersensitive to the Rho GTPase Rac2 protein","volume":"291","author":"Walliser","year":"2016","journal-title":"J Biol Chem"},{"key":"10.1016\/j.slasd.2023.03.003_bib0019","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1182\/blood-2015-02-626846","article-title":"Hypermorphic mutation of phospholipase C, \u03b32 acquired in ibrutinib-resistant CLL confers BTK independency upon B-cell receptor activation","volume":"126","author":"Liu","year":"2015","journal-title":"Blood"},{"key":"10.1016\/j.slasd.2023.03.003_bib0020","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.3109\/10428194.2014.941832","article-title":"Expression and pro-survival function of phospholipase C\u03b32 in diffuse large B-cell lymphoma","volume":"56","author":"Huynh","year":"2015","journal-title":"Leuk Lymphoma"},{"key":"10.1016\/j.slasd.2023.03.003_bib0021","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0150411","article-title":"A gain-of-function mutation in the Plcg2 gene protects mice from helicobacter felis-induced gastric MALT lymphoma","volume":"11","author":"Gossmann","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.slasd.2023.03.003_bib0022","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nmeth867","article-title":"Analyzing phosphoinositides and their interacting proteins","volume":"3","author":"Rusten","year":"2006","journal-title":"Nat Methods"},{"key":"10.1016\/j.slasd.2023.03.003_bib0023","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1054\/ceca.1999.0095","article-title":"Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes","volume":"27","author":"Gee","year":"2000","journal-title":"Cell Calcium"},{"key":"10.1016\/j.slasd.2023.03.003_bib0024","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1021\/cb100308n","article-title":"Small molecule reporter for mammalian phospholipase C isozymes","volume":"6","author":"Huang","year":"2011","journal-title":"ACS Chem Biol"},{"key":"10.1016\/j.slasd.2023.03.003_bib0025","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1074\/jbc.RA117.000926","article-title":"A membrane-associated, fluorogenic reporter for mammalian phospholipase C isozymes","volume":"293","author":"Huang","year":"2018","journal-title":"J Biol Chem"},{"key":"10.1016\/j.slasd.2023.03.003_bib0026","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.51700","article-title":"Structural basis for the activation of PLC-\u03b3 isozymes by phosphorylation and cancer-associated mutations","volume":"8","author":"Hajicek","year":"2019","journal-title":"eLife"},{"key":"10.1016\/j.slasd.2023.03.003_bib0027","doi-asserted-by":"crossref","first-page":"5840","DOI":"10.1074\/jbc.M112.422501","article-title":"Small molecule inhibitors of phospholipase C from a novel high-throughput screen","volume":"288","author":"Huang","year":"2013","journal-title":"J Biol Chem"},{"key":"10.1016\/j.slasd.2023.03.003_bib0028","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abp9688","article-title":"Characterization of the membrane interactions of phospholipase C\u03b3 reveals key features of the active enzyme","volume":"8","author":"Le Huray","year":"2022","journal-title":"Sci Adv"},{"key":"10.1016\/j.slasd.2023.03.003_bib0029","doi-asserted-by":"crossref","first-page":"4029","DOI":"10.1021\/acs.biochem.0c00511","article-title":"A high-throughput assay to identify allosteric inhibitors of the PLC-\u03b3 isozymes operating at membranes","volume":"59","author":"Huang","year":"2020","journal-title":"Biochemistry"},{"key":"10.1016\/j.slasd.2023.03.003_bib0030","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1021\/op0000529","article-title":"High selectivity in the oxidation of mandelic acid derivatives and in O-methylation of protocatechualdehyde: new processes for synthesis of vanillin, iso-vanillin, and heliotropin","volume":"4","author":"Bj\u00f8rsvik","year":"2000","journal-title":"Org Process Res Dev"},{"key":"10.1016\/j.slasd.2023.03.003_bib0031","first-page":"225","author":"Carr","year":"2021","journal-title":"Fluorogenic XY-69 in lipid vesicles for measuring activity of phospholipase C isozymes"},{"key":"10.1016\/j.slasd.2023.03.003_bib0032","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s13024-020-00402-7","article-title":"The Alzheimer's disease-associated protective Plc\u03b32-P522R variant promotes immune functions","volume":"15","author":"Takalo","year":"2020","journal-title":"Mol Neurodegener"},{"key":"10.1016\/j.slasd.2023.03.003_bib0033","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nchembio814","article-title":"Small-molecule activation of procaspase-3 to caspase-3 as a personalized anticancer strategy","volume":"2","author":"Putt","year":"2006","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.slasd.2023.03.003_bib0034","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.ab.2004.07.017","article-title":"Noninvasive methods to determine the critical micelle concentration of some bile acid salts","volume":"334","author":"Reis","year":"2004","journal-title":"Anal Biochem"},{"key":"10.1016\/j.slasd.2023.03.003_bib0035","first-page":"2835","article-title":"Selective inhibition of phosphatidylinositol phospholipase C by cytotoxic ether lipid analogues","volume":"52","author":"Powis","year":"1992","journal-title":"Cancer Res"}],"container-title":["SLAS Discovery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555223000242?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555223000242?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T11:56:10Z","timestamp":1762862170000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2472555223000242"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":35,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["S2472555223000242"],"URL":"https:\/\/doi.org\/10.1016\/j.slasd.2023.03.003","relation":{},"ISSN":["2472-5552"],"issn-type":[{"value":"2472-5552","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel fluorogenic reporter substrate for 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (PLC\u03b32): Application to high-throughput screening for activators to treat Alzheimer's disease","name":"articletitle","label":"Article Title"},{"value":"SLAS Discovery","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.slasd.2023.03.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Author(s). Published by Elsevier Inc. on behalf of Society for Laboratory Automation and Screening.","name":"copyright","label":"Copyright"}]}}