{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:39:05Z","timestamp":1791394745819,"version":"4.3.3"},"reference-count":25,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T00:00:00Z","timestamp":1730160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019188","name":"HORIZON EUROPE Excellent Science","doi-asserted-by":"publisher","award":["675179"],"award-info":[{"award-number":["675179"]}],"id":[{"id":"10.13039\/100019188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["slas-discovery.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["SLAS Discovery"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1016\/j.slasd.2024.100191","type":"journal-article","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T18:50:36Z","timestamp":1730832636000},"page":"100191","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of a live cell assay for real-time monitoring the interactions between the Hippo pathway components 14-3-3 and TAZ"],"prefix":"10.1016","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0838-3213","authenticated-orcid":false,"given":"Bla\u017e","family":"Andlovic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8822-4371","authenticated-orcid":false,"given":"Alexander","family":"Wolf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malgorzata","family":"Hiltmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bert M.","family":"Klebl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5537-438X","authenticated-orcid":false,"given":"Jan","family":"Eickhoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7315-0315","authenticated-orcid":false,"given":"Christian","family":"Ottmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"7","key":"10.1016\/j.slasd.2024.100191_bib0001","doi-asserted-by":"crossref","first-page":"2426","DOI":"10.1128\/MCB.01874-07","article-title":"TAZ promotes cell proliferation and epithelial-mesenchymal transition and is inhibited by the hippo pathway","volume":"28","author":"Lei","year":"2008","journal-title":"Mol Cell Biol"},{"issue":"5","key":"10.1016\/j.slasd.2024.100191_bib0002","doi-asserted-by":"crossref","first-page":"390","DOI":"10.4161\/cbt.23788","article-title":"Emerging roles of TEAD transcription factors and its coactivators in cancers","volume":"14","author":"Pobbati","year":"2013","journal-title":"Cancer Biol Ther"},{"issue":"3","key":"10.1016\/j.slasd.2024.100191_bib0003","doi-asserted-by":"crossref","first-page":"106","DOI":"10.5483\/BMBRep.2018.51.3.022","article-title":"The history and regulatory mechanism of the hippo pathway","volume":"51","author":"Kim","year":"2018","journal-title":"BMB Rep"},{"key":"10.1016\/j.slasd.2024.100191_bib0004","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.canlet.2021.12.019","article-title":"Context-dependent transcriptional regulations of YAP\/TAZ in cancer","volume":"527","author":"Guo","year":"2022","journal-title":"Cancer Lett"},{"issue":"1","key":"10.1016\/j.slasd.2024.100191_bib0005","doi-asserted-by":"crossref","first-page":"27459","DOI":"10.1038\/srep27459","article-title":"Cloning: a single-tube universal cloning system exploiting bacterial in vivo assembly","volume":"6","author":"Garc\u00eda-Nafr\u00eda","year":"2016","journal-title":"Sci Rep"},{"issue":"1","key":"10.1016\/j.slasd.2024.100191_bib0006","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1146\/annurev.pharmtox.40.1.617","article-title":"14-3-3 proteins: structure, function, and regulation","volume":"40","author":"Fu","year":"2000","journal-title":"Annu Rev Pharmacol Toxicol"},{"issue":"48","key":"10.1016\/j.slasd.2024.100191_bib0007","doi-asserted-by":"crossref","first-page":"37159","DOI":"10.1074\/jbc.M110.152942","article-title":"The hippo tumor pathway promotes TAZ degradation by phosphorylating a phosphodegron and recruiting the SCF{beta}-TrCP E3 ligase","volume":"285","author":"Liu","year":"2010","journal-title":"J Biol Chem"},{"issue":"31","key":"10.1016\/j.slasd.2024.100191_bib0008","doi-asserted-by":"crossref","first-page":"26245","DOI":"10.1074\/jbc.M112.382036","article-title":"The N-Terminal phosphodegron targets TAZ\/WWTR1 protein for SCF\u03b2-TrCP-dependent degradation in response to phosphatidylinositol 3-Kinase inhibition","volume":"287","author":"Huang","year":"2012","journal-title":"J Biol Chem"},{"issue":"7","key":"10.1016\/j.slasd.2024.100191_bib0009","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1016\/j.cell.2012.11.027","article-title":"Role of TAZ as mediator of Wnt signaling","volume":"151","author":"Azzolin","year":"2012","journal-title":"Cell"},{"issue":"D1","key":"10.1016\/j.slasd.2024.100191_bib0010","doi-asserted-by":"crossref","first-page":"D917","DOI":"10.1093\/nar\/gky1129","article-title":"canSAR: update to the cancer translational research and drug discovery knowledgebase","volume":"47","author":"Coker","year":"2019","journal-title":"Nucleic Acids Res"},{"issue":"31","key":"10.1016\/j.slasd.2024.100191_bib0011","doi-asserted-by":"crossref","first-page":"31399","DOI":"10.18632\/oncotarget.5189","article-title":"CDK1 phosphorylation of TAZ in mitosis inhibits its oncogenic activity","volume":"6","author":"Zhang","year":"2015","journal-title":"Oncotarget"},{"issue":"1\u20132","key":"10.1016\/j.slasd.2024.100191_bib0012","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1101\/gad.333146.119","article-title":"CDK7 regulates organ size and tumor growth by safeguarding the hippo pathway effector Yki\/Yap\/Taz in the nucleus","volume":"34","author":"Cho","year":"2020","journal-title":"Genes Dev"},{"issue":"1","key":"10.1016\/j.slasd.2024.100191_bib0013","doi-asserted-by":"crossref","first-page":"61","DOI":"10.15252\/embr.201642683","article-title":"Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization","volume":"18","author":"Moon","year":"2017","journal-title":"EMBO Rep"},{"issue":"14","key":"10.1016\/j.slasd.2024.100191_bib0014","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.1038\/onc.2011.363","article-title":"Integrating developmental signals: a hippo in the (Path)Way","volume":"31","author":"Mauviel","year":"2012","journal-title":"Oncogene"},{"issue":"3","key":"10.1016\/j.slasd.2024.100191_bib0015","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.celrep.2015.09.014","article-title":"Tankyrase inhibitors target YAP by stabilizing angiomotin family proteins","volume":"13","author":"Wang","year":"2015","journal-title":"Cell Rep"},{"issue":"7","key":"10.1016\/j.slasd.2024.100191_bib0016","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1073\/pnas.1216462110","article-title":"Regulation of Hippo Pathway by Mitogenic Growth Factors via Phosphoinositide 3-Kinase and Phosphoinositide-Dependent Kinase-1","volume":"110","author":"Fan","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"3","key":"10.1016\/j.slasd.2024.100191_bib0017","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/s41419-018-0302-x","article-title":"EGFR-PI3K-PDK1 pathway regulates YAP signaling in hepatocellular carcinoma: the mechanism and its implications in targeted therapy","volume":"9","author":"Xia","year":"2018","journal-title":"Cell Death Dis"},{"issue":"1","key":"10.1016\/j.slasd.2024.100191_bib0018","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1186\/s13046-018-0989-7","article-title":"Inhibition of Stearoyl-CoA Desaturase 1 reverts BRAF and MEK inhibition-induced selection of cancer stem cells in BRAF-mutated melanoma","volume":"37","author":"Pisanu","year":"2018","journal-title":"J Exp Clin Cancer Res"},{"issue":"3","key":"10.1016\/j.slasd.2024.100191_bib0019","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1038\/ng.3218","article-title":"The hippo effector YAP promotes resistance to RAF- and MEK-targeted cancer therapies","volume":"47","author":"Lin","year":"2015","journal-title":"Nat. Genet."},{"issue":"5","key":"10.1016\/j.slasd.2024.100191_bib0020","doi-asserted-by":"crossref","first-page":"462","DOI":"10.15252\/embj.201592081","article-title":"Actin remodeling confers BRAF inhibitor resistance to melanoma cells through YAP\/TAZ activation","volume":"35","author":"Kim","year":"2016","journal-title":"EMBO J"},{"issue":"4","key":"10.1016\/j.slasd.2024.100191_bib0021","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1161\/HYPERTENSIONAHA.119.12834","article-title":"Regulation of YAP by mammalian target of rapamycin complex 1 in endothelial cells controls blood pressure through COX-2\/mPGES-1\/PGE (2) cascade","volume":"74","author":"Yao","year":"2019","journal-title":"Hypertension"},{"issue":"13","key":"10.1016\/j.slasd.2024.100191_bib0022","doi-asserted-by":"crossref","first-page":"3634","DOI":"10.7150\/ijbs.61795","article-title":"Autophagy-mediated negative feedback attenuates the oncogenic activity of YAP in pancreatic cancer","volume":"17","author":"Sun","year":"2021","journal-title":"Int J Biol Sci"},{"issue":"11","key":"10.1016\/j.slasd.2024.100191_bib0023","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1084\/jem.20140341","article-title":"Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex","volume":"211","author":"Liang","year":"2014","journal-title":"J Exp Med"},{"issue":"06","key":"10.1016\/j.slasd.2024.100191_bib0024","article-title":"Covalent 14-3-3 molecular glues and heterobifunctional molecules against nuclear transcription factors and regulators","volume":"11","author":"Shao","year":"2023","journal-title":"bioRxiv 2023"},{"issue":"13","key":"10.1016\/j.slasd.2024.100191_bib0025","doi-asserted-by":"crossref","first-page":"6694","DOI":"10.1021\/acs.jmedchem.9b01942","article-title":"Fragment-based differential targeting of PPI stabilizer interfaces","volume":"63","author":"Guillory","year":"2020","journal-title":"J Med Chem"}],"container-title":["SLAS Discovery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555224000534?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555224000534?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T09:40:10Z","timestamp":1734428410000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2472555224000534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":25,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["S2472555224000534"],"URL":"https:\/\/doi.org\/10.1016\/j.slasd.2024.100191","relation":{},"ISSN":["2472-5552"],"issn-type":[{"value":"2472-5552","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a live cell assay for real-time monitoring the interactions between the Hippo pathway components 14-3-3 and TAZ","name":"articletitle","label":"Article Title"},{"value":"SLAS Discovery","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.slasd.2024.100191","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier Inc. on behalf of Society for Laboratory Automation and Screening.","name":"copyright","label":"Copyright"}],"article-number":"100191"}}