{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T22:12:17Z","timestamp":1788387137003,"version":"build-2803163510"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T00:00:00Z","timestamp":1756252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013114","name":"Broad Institute","doi-asserted-by":"publisher","award":["NIH OD-026,839\\u201301"],"award-info":[{"award-number":["NIH OD-026,839\\u201301"]}],"id":[{"id":"10.13039\/100013114","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005292","name":"Brigham and Women&apos;s Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005292","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000898","name":"The Eli and Edythe Broad Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000898","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014989","name":"The Chan Zuckerberg Initiative","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["MIRA R35 GM122547"],"award-info":[{"award-number":["MIRA R35 GM122547"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["slas-discovery.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["SLAS Discovery"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.slasd.2025.100268","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T16:26:16Z","timestamp":1756398376000},"page":"100268","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Alternate dyes for image-based profiling assays"],"prefix":"10.1016","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9778-5400","authenticated-orcid":false,"given":"Suganya","family":"Sivagurunathan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2569-6019","authenticated-orcid":false,"given":"Patrick","family":"Byrne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Al\u00e1n F.","family":"Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Arevalo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1555-8261","authenticated-orcid":false,"given":"Anne E.","family":"Carpenter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shantanu","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Kost-Alimova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9640-9318","authenticated-orcid":false,"given":"Beth A.","family":"Cimini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9","key":"10.1016\/j.slasd.2025.100268_bib0001","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1038\/nprot.2016.105","article-title":"Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes","volume":"11","author":"Bray","year":"2016","journal-title":"Nat Protoc"},{"issue":"7","key":"10.1016\/j.slasd.2025.100268_bib0002","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1038\/s41596-023-00840-9","article-title":"Optimizing the Cell painting assay for image-based profiling","volume":"18","author":"Cimini","year":"2023","journal-title":"Nat Protoc"},{"issue":"2","key":"10.1016\/j.slasd.2025.100268_bib0003","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1038\/s41573-020-00117-w","article-title":"Image-based profiling for drug discovery: due for a machine-learning upgrade?","volume":"20","author":"Chandrasekaran","year":"2021","journal-title":"Nat Rev Drug Discov"},{"issue":"10","key":"10.1016\/j.slasd.2025.100268_bib0004","doi-asserted-by":"crossref","first-page":"R100","DOI":"10.1186\/gb-2006-7-10-r100","article-title":"CellProfiler: image analysis software for identifying and quantifying cell phenotypes","volume":"7","author":"Carpenter","year":"2006","journal-title":"Genome Biol"},{"issue":"3","key":"10.1016\/j.slasd.2025.100268_bib0005","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1161\/CIRCULATIONAHA.114.010403","article-title":"Strategy for identifying repurposed drugs for the treatment of cerebral cavernous malformation","volume":"131","author":"Gibson","year":"2015","journal-title":"Circulation"},{"issue":"114876","key":"10.1016\/j.slasd.2025.100268_bib0006","article-title":"Bioactivity screening of environmental chemicals using imaging-based high-throughput phenotypic profiling","volume":"389","author":"Nyffeler","year":"2020","journal-title":"Toxicol Appl Pharmacol"},{"issue":"6","key":"10.1016\/j.slasd.2025.100268_bib0007","doi-asserted-by":"crossref","first-page":"ar49","DOI":"10.1091\/mbc.E21-11-0538","article-title":"Cell painting predicts impact of lung cancer variants","volume":"33","author":"Caicedo","year":"2022","journal-title":"Mol Biol Cell"},{"key":"10.1016\/j.slasd.2025.100268_bib0008","first-page":"1","article-title":"Cell Painting: a decade of discovery and innovation in cellular imaging","author":"Seal","year":"2024","journal-title":"Nat Methods"},{"issue":"6","key":"10.1016\/j.slasd.2025.100268_bib0009","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2020.108371","article-title":"TFEB transcriptional responses reveal negative feedback by BHLHE40 and BHLHE41","volume":"33","author":"Carey","year":"2020","journal-title":"Cell Rep"},{"issue":"7","key":"10.1016\/j.slasd.2025.100268_bib0010","article-title":"Discovering cellular programs of intrinsic and extrinsic drivers of metabolic traits using LipocyteProfiler","volume":"3","author":"Laber","year":"2023","journal-title":"Cell Genom"},{"issue":"3","key":"10.1016\/j.slasd.2025.100268_bib0011","doi-asserted-by":"crossref","DOI":"10.1016\/j.slasd.2023.10.005","article-title":"Live cell painting: new nontoxic dye to probe cell physiology in high content screening","volume":"29","author":"Cottet","year":"2024","journal-title":"SLAS Discovery"},{"key":"10.1016\/j.slasd.2025.100268_bib0012","doi-asserted-by":"crossref","DOI":"10.1091\/mbc.E24-07-0308","article-title":"Live Cell Painting: image-based profiling in live cells using Acridine Orange","author":"Garcia-Fossa","year":"2025","journal-title":"Mol. Biol. Cell"},{"issue":"11","key":"10.1016\/j.slasd.2025.100268_bib0013","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1002\/cyto.a.24786","article-title":"Assessing the performance of the Cell painting assay across different imaging systems","volume":"103","author":"Tromans-Coia","year":"2023","journal-title":"Cytometry A"},{"key":"10.1016\/j.slasd.2025.100268_bib0014","first-page":"1","article-title":"Three million images and morphological profiles of cells treated with matched chemical and genetic perturbations","author":"Chandrasekaran","year":"2024","journal-title":"Nat Methods"},{"issue":"9","key":"10.1016\/j.slasd.2025.100268_bib0015","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1091\/mbc.E20-12-0784","article-title":"Predicting cell health phenotypes using image-based morphology profiling","volume":"32","author":"Way","year":"2021","journal-title":"Mol Biol Cell"},{"key":"10.1016\/j.slasd.2025.100268_bib0016","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-025-60306-2","article-title":"A versatile information retrieval framework for evaluating profile strength and similarity","author":"Kalinin","year":"2025","journal-title":"Nat Commun"},{"issue":"8","key":"10.1016\/j.slasd.2025.100268_bib0017","doi-asserted-by":"crossref","DOI":"10.1002\/bies.201700003","article-title":"Phototoxicity in live fluorescence microscopy, and how to avoid it","volume":"39","author":"Icha","year":"2017","journal-title":"Bioessays"},{"issue":"1","key":"10.1016\/j.slasd.2025.100268_bib0018","doi-asserted-by":"crossref","first-page":"6516","DOI":"10.1038\/s41467-024-50613-5","article-title":"Evaluating batch correction methods for image-based cell profiling","volume":"15","author":"Arevalo","year":"2024","journal-title":"Nat Commun"},{"issue":"10","key":"10.1016\/j.slasd.2025.100268_bib0019","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1177\/1087057113500812","article-title":"Phenotypic screening with human iPS cell-derived cardiomyocytes: hTS-compatible assays for interrogating cardiac hypertrophy","volume":"18","author":"Carlson","year":"2013","journal-title":"J Biomol Screen"},{"issue":"2","key":"10.1016\/j.slasd.2025.100268_bib0020","doi-asserted-by":"crossref","first-page":"fcae101","DOI":"10.1093\/braincomms\/fcae101","article-title":"Morphological profiling in human neural progenitor cells classifies hits in a pilot drug screen for Alzheimer\u2019s disease","volume":"6","author":"McDiarmid","year":"2024","journal-title":"Brain Commun"},{"issue":"7","key":"10.1016\/j.slasd.2025.100268_bib0021","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1011323","article-title":"Evaluating the utility of brightfield image data for mechanism of action prediction","volume":"19","author":"Harrison","year":"2023","journal-title":"PLoS Comput Biol"},{"issue":"1","key":"10.1016\/j.slasd.2025.100268_bib0022","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-022-12914-x","article-title":"Label-free prediction of cell painting from brightfield images","volume":"12","author":"Cross-Zamirski","year":"2022","journal-title":"Sci Rep"},{"key":"10.1016\/j.slasd.2025.100268_bib0023","first-page":"1","article-title":"Cell Painting Gallery: an open resource for image-based profiling","author":"Weisbart","year":"2024","journal-title":"Nat Methods"},{"issue":"12","key":"10.1016\/j.slasd.2025.100268_bib0024","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1038\/s41592-022-01663-4","article-title":"Cellpose 2.0: how to train your own model","volume":"19","author":"Pachitariu","year":"2022","journal-title":"Nat Methods"},{"key":"10.1016\/j.slasd.2025.100268_bib0025","article-title":"Reproducible image-based profiling with Pycytominer","author":"Serrano","year":"2023","journal-title":"arXiv [q-bio.QM]"},{"key":"10.1016\/j.slasd.2025.100268_bib0026","series-title":"Positioning and power in academic publishing: players, agents and agendas","author":"Kluyver","year":"2016"},{"key":"10.1016\/j.slasd.2025.100268_bib0027","unstructured":"Reback J., McKinney W., jbrockmendel, Van den Bossche J., Augspurger T., Cloud P., et al. pandas-dev\/pandas: pandas 1.0.3 [Internet]. 2020. Available from: https:\/\/zenodo.org\/record\/3715232."},{"issue":"7825","key":"10.1016\/j.slasd.2025.100268_bib0028","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/s41586-020-2649-2","article-title":"Array programming with NumPy","volume":"585","author":"Harris","year":"2020","journal-title":"Nature"},{"issue":"60","key":"10.1016\/j.slasd.2025.100268_bib0029","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.21105\/joss.03021","article-title":"seaborn: statistical data visualization","volume":"6","author":"Waskom","year":"2021","journal-title":"J Open Source Softw"},{"key":"10.1016\/j.slasd.2025.100268_bib0030","unstructured":"Inc PT. Collaborative data science. Montr\u00e9al, QC [Internet]. 2015; Available from: https:\/\/plot.ly."}],"container-title":["SLAS Discovery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555225000619?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555225000619?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T20:06:20Z","timestamp":1772913980000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2472555225000619"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":30,"alternative-id":["S2472555225000619"],"URL":"https:\/\/doi.org\/10.1016\/j.slasd.2025.100268","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.02.19.639058","asserted-by":"object"}]},"ISSN":["2472-5552"],"issn-type":[{"value":"2472-5552","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Alternate dyes for image-based profiling assays","name":"articletitle","label":"Article Title"},{"value":"SLAS Discovery","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.slasd.2025.100268","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Broad Institute, Inc. Published by Elsevier Inc. on behalf of Society for Laboratory Automation and Screening.","name":"copyright","label":"Copyright"}],"article-number":"100268"}}