{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:00:39Z","timestamp":1789203639084,"version":"build-2803163510"},"reference-count":39,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","thespinejournalonline.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Spine Journal"],"published-print":{"date-parts":[[2015,6]]},"DOI":"10.1016\/j.spinee.2015.02.034","type":"journal-article","created":{"date-parts":[[2015,2,28]],"date-time":"2015-02-28T08:05:59Z","timestamp":1425110759000},"page":"1379-1390","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":43,"title":["The bone morphogenetic protein-2\/7 heterodimer is a stronger inducer of bone regeneration than the individual homodimers in a rat spinal fusion model"],"prefix":"10.1016","volume":"15","author":[{"given":"Tokimitsu","family":"Morimoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Kaito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Matsuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuyoshi","family":"Sugiura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masafumi","family":"Kashii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Makino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoki","family":"Iwasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Yoshikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.spinee.2015.02.034_bib1","first-page":"80","article-title":"Pseudoarthrosis of the spine","volume":"284","author":"Steinmann","year":"1993","journal-title":"Clin Orthop"},{"key":"10.1016\/j.spinee.2015.02.034_bib2","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1097\/00007632-199306150-00006","article-title":"A prospective, randomized study of lumbar fusion","volume":"18","author":"Zdeblick","year":"1993","journal-title":"Spine"},{"key":"10.1016\/j.spinee.2015.02.034_bib3","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1126\/science.3201241","article-title":"Novel regulators of bone formation: molecular clones and activities","volume":"242","author":"Wozney","year":"1998","journal-title":"Science"},{"key":"10.1016\/j.spinee.2015.02.034_bib4","doi-asserted-by":"crossref","first-page":"2220","DOI":"10.1073\/pnas.87.6.2220","article-title":"Recombinant human bone morphogenetic protein induces bone formation","volume":"87","author":"Wang","year":"1990","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.spinee.2015.02.034_bib5","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1126\/science.150.3698.893","article-title":"Bone formation by autoinduction","volume":"150","author":"Urist","year":"1965","journal-title":"Science"},{"key":"10.1016\/j.spinee.2015.02.034_bib6","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.cytogfr.2005.05.001","article-title":"Delivery of bone morphogenetic proteins for orthopedic tissue regeneration","volume":"16","author":"Seeherman","year":"2005","journal-title":"Cytokine Growth Factor Rev"},{"key":"10.1016\/j.spinee.2015.02.034_bib7","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1097\/00007632-200208151-00017","article-title":"Economic evaluation of bone morphogenetic protein versus autogenous iliac crest bone graft in single-level anterior lumbar fusion: an evidence-based modeling approach","volume":"27","author":"Ackerman","year":"2002","journal-title":"Spine"},{"key":"10.1016\/j.spinee.2015.02.034_bib8","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1001\/jama.2009.956","article-title":"Prevalence, complications, and hospital charges associated with use of bone-morphogenetic proteins in spinal fusion procedures","volume":"302","author":"Cahill","year":"2009","journal-title":"JAMA"},{"key":"10.1016\/j.spinee.2015.02.034_bib9","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1097\/01.brs.0000201424.27509.72","article-title":"Adverse effects associated with high-dose recombinant human bone morphogenetic protein-2 use in anterior cervical spine fusion","volume":"31","author":"Shields","year":"2006","journal-title":"Spine"},{"key":"10.1016\/j.spinee.2015.02.034_bib10","doi-asserted-by":"crossref","first-page":"2813","DOI":"10.1097\/01.brs.0000245863.52371.c2","article-title":"Increased swelling complications associated with off-label usage of rhBMP-2 in the anterior cervical spine","volume":"31","author":"Smucker","year":"2006","journal-title":"Spine"},{"key":"10.1016\/j.spinee.2015.02.034_bib11","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1097\/00024720-200302000-00014","article-title":"The effects of BMP-7 in a rat posterolateral intertransverse process fusion model","volume":"16","author":"Salamon","year":"2003","journal-title":"J Spinal Disord Tech"},{"key":"10.1016\/j.spinee.2015.02.034_bib12","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1097\/01.brs.0000172230.01655.55","article-title":"Estrogen-dependent actions of bone morphogenetic protein-7 on spine fusion in rats","volume":"30","author":"Moazzaz","year":"2005","journal-title":"Spine"},{"key":"10.1016\/j.spinee.2015.02.034_bib13","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1097\/BRS.0b013e3181cc0220","article-title":"Bone morphogenetic protein binding peptide mechanism and enhancement of osteogenic protein-1 inducedbone healing","volume":"35","author":"Taghavi","year":"2010","journal-title":"Spine"},{"key":"10.1016\/j.spinee.2015.02.034_bib14","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1097\/BRS.0b013e31817e9dfd","article-title":"The adjunctive effect of a binding peptide on bone morphogenetic protein enhanced bonehealing in a rodent model of spinal fusion","volume":"33","author":"Alanay","year":"2008","journal-title":"Spine"},{"key":"10.1016\/j.spinee.2015.02.034_bib15","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1002\/jor.21454","article-title":"Nanocomposite therapy as a more efficacious and less inflammatory alternative to bone morphogenetic protein-2 in a rodent arthrodesis model","volume":"29","author":"Hsu","year":"2011","journal-title":"J Orthop Res"},{"key":"10.1016\/j.spinee.2015.02.034_bib16","doi-asserted-by":"crossref","first-page":"e107","DOI":"10.2106\/JBJS.M.01097","article-title":"Effect of intermittent administration of Teriparatide (PTH1-34) on BMP-induced bone formation in a rat spinal fusion model","volume":"96","author":"Morimoto","year":"2014","journal-title":"J Bone Joint Surg Am"},{"key":"10.1016\/j.spinee.2015.02.034_bib17","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1002\/jbmr.2213","article-title":"A novel osteogenic osysterol compound for therapeutic development to promote bone growth: activation of hedgehog signaling and osteogenesis through smoothened binding","volume":"29","author":"Montgomery","year":"2014","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.spinee.2015.02.034_bib18","doi-asserted-by":"crossref","first-page":"291","DOI":"10.3109\/08977199609003229","article-title":"Heterodimeric bone morphogenetic proteins show enhanced activity in\u00a0vitro and in\u00a0vivo","volume":"13","author":"Israel","year":"1996","journal-title":"Growth Factors"},{"key":"10.1016\/j.spinee.2015.02.034_bib19","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1006\/bbrc.1995.1712","article-title":"Potent ectopic bone-inducing activity of bone morphogenetic protein-4\/7 heterodimer","volume":"210","author":"Aono","year":"1995","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.spinee.2015.02.034_bib20","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1006\/bbrc.1995.1578","article-title":"Efficient expression of a heterodimer of bone morphogenetic protein subunits using a baculovirus expression system","volume":"209","author":"Hazama","year":"1995","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.spinee.2015.02.034_bib21","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1359\/jbmr.040821","article-title":"Combined bone morphogenetic protein-2 and -7 gene transfer enhances osteoblastic differentiation and spine fusion in a rodent model","volume":"19","author":"Zhu","year":"2004","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.spinee.2015.02.034_bib22","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1177\/154405910808700906","article-title":"Combinatorial gene therapy with BMP2\/7 enhances cranial bone regeneration","volume":"87","author":"Koh","year":"2008","journal-title":"J Dent Res"},{"key":"10.1016\/j.spinee.2015.02.034_bib23","doi-asserted-by":"crossref","first-page":"239","DOI":"10.4012\/dmj.2011-191","article-title":"BMP2\/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer","volume":"31","author":"Sun","year":"2012","journal-title":"Dent Mater J"},{"key":"10.1016\/j.spinee.2015.02.034_bib24","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.2106\/JBJS.L.01396","article-title":"Synergistic effect of bone morphogenetic proteins 2 and 7 by ex\u00a0vivo gene therapy in a rat spinal fusion model","volume":"95","author":"Kaito","year":"2013","journal-title":"J Bone Joint Surg Am"},{"key":"10.1016\/j.spinee.2015.02.034_bib25","doi-asserted-by":"crossref","first-page":"303","DOI":"10.4103\/0976-500X.119726","article-title":"How to calculate sample size in animal studies?","volume":"4","author":"Charan","year":"2013","journal-title":"J Pharmacol Pharmacother"},{"key":"10.1016\/j.spinee.2015.02.034_bib26","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1097\/BRS.0b013e31816b45c3","article-title":"Comparison of human mesenchymal stem cells derived from adipose tissue and bone marrow for ex\u00a0vivo gene therapy in rat spinal fusion model","volume":"33","author":"Miyazaki","year":"2008","journal-title":"Spine"},{"key":"10.1016\/j.spinee.2015.02.034_bib27","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1097\/BRS.0b013e31824ac089","article-title":"Dose-dependent enhancement of spinal fusion in rats with teriparatide (PTH[1-34])","volume":"37","author":"Ming","year":"2012","journal-title":"Spine"},{"key":"10.1016\/j.spinee.2015.02.034_bib28","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.bone.2007.06.025","article-title":"Enhancement of graft bone healing by intermittent administration of human parathyroid hormone (1-34) in a rat spinal arthrodesis model","volume":"41","author":"Abe","year":"2007","journal-title":"Bone"},{"key":"10.1016\/j.spinee.2015.02.034_bib29","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.spinee.2008.08.009","article-title":"A porcine collagen-derived matrix as a carrier for recombinant human bone morphogenetic protein-2 enhances spinal fusion in rats","volume":"9","author":"Miyazaki","year":"2009","journal-title":"Spine J"},{"key":"10.1016\/j.spinee.2015.02.034_bib30","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1083\/jcb.200311015","article-title":"Smad6\/Smurf1 overexpression in cartilage delays chondrocyte hypertrophy and caused dwarfism with osteopenica","volume":"165","author":"Horiki","year":"2004","journal-title":"J Cell Biol"},{"key":"10.1016\/j.spinee.2015.02.034_bib31","doi-asserted-by":"crossref","first-page":"905","DOI":"10.2106\/00004623-200305000-00020","article-title":"Effect of regional gene therapy with bone morphogenetic protein-2-producing bone marrow cells on spinal fusion in rats","volume":"85-A","author":"Wang","year":"2003","journal-title":"J Bone Joint Surg Am"},{"key":"10.1016\/j.spinee.2015.02.034_bib32","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1097\/BRS.0b013e318162451b","article-title":"Posterolateral intertransverse spinal fusion possible in osteoporotic rats with BMP-7 in a higher dose delivered on composite carrier","volume":"33","author":"Lu","year":"2008","journal-title":"Spine"},{"key":"10.1016\/j.spinee.2015.02.034_bib33","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.cellsig.2003.08.011","article-title":"Signal transduction of bone morphogenetic protein receptors","volume":"16","author":"Nohe","year":"2004","journal-title":"Cell Signal"},{"key":"10.1016\/j.spinee.2015.02.034_bib34","doi-asserted-by":"crossref","first-page":"16985","DOI":"10.1016\/S0021-9258(17)32506-1","article-title":"Identification of type I receptors for osteogenic protein-1 and bone morphogenetic protein-4","volume":"269","author":"ten Dijke","year":"1994","journal-title":"J Biol Chem"},{"key":"10.1016\/j.spinee.2015.02.034_bib35","doi-asserted-by":"crossref","first-page":"21345","DOI":"10.1074\/jbc.271.35.21345","article-title":"Identification of type I and type II serine\/threonine kinase receptors for growth\/differentiation factor-5","volume":"271","author":"Nishitoh","year":"1996","journal-title":"J Biol Chem"},{"key":"10.1016\/j.spinee.2015.02.034_bib36","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1083\/jcb.130.1.217","article-title":"Osteogenic protein-1 binds to activin type II receptors and induces certain activin-like effects","volume":"130","author":"Yamashita","year":"1995","journal-title":"J Cell Biol"},{"key":"10.1016\/j.spinee.2015.02.034_bib37","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1210\/me.2009-0496","article-title":"Bone morphogenetic protei-2 and -6 heterodimer illustrates the nature of ligand-receptor assembly","volume":"24","author":"Isaacs","year":"2010","journal-title":"Mol Endocrinol"},{"key":"10.1016\/j.spinee.2015.02.034_bib38","doi-asserted-by":"crossref","first-page":"e11167","DOI":"10.1371\/journal.pone.0011167","article-title":"BMP-2\/6 heterodimer is more effective than BMP-2 or BMP-6 homodimers as inductor of differentiation of human embryonic stem cells","volume":"5","author":"Valera","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.spinee.2015.02.034_bib39","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.bone.2005.12.018","article-title":"Noggin regulation of bone morphogenetic protein (BMP) 2\/7 heterodimer activity in\u00a0vitro","volume":"39","author":"Zhu","year":"2006","journal-title":"Bone"}],"container-title":["The Spine Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1529943015002004?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1529943015002004?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T02:19:00Z","timestamp":1651371540000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1529943015002004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["S1529943015002004"],"URL":"https:\/\/doi.org\/10.1016\/j.spinee.2015.02.034","relation":{},"ISSN":["1529-9430"],"issn-type":[{"value":"1529-9430","type":"print"}],"subject":[],"published":{"date-parts":[[2015,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The bone morphogenetic protein-2\/7 heterodimer is a stronger inducer of bone regeneration than the individual homodimers in a rat spinal fusion model","name":"articletitle","label":"Article Title"},{"value":"The Spine Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.spinee.2015.02.034","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}