{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T12:06:54Z","timestamp":1794053214041,"version":"4.8.0"},"reference-count":95,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":370,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000900","name":"California Institute for Regenerative Medicine","doi-asserted-by":"publisher","award":["T32HD060549"],"award-info":[{"award-number":["T32HD060549"]}],"id":[{"id":"10.13039\/100000900","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009020","name":"University of South China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009020","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["DP2DK135739"],"award-info":[{"award-number":["DP2DK135739"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["DK054364"],"award-info":[{"award-number":["DK054364"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006034","name":"University of Southern California","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006034","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Stem Cell"],"published-print":{"date-parts":[[2024,6]]},"DOI":"10.1016\/j.stem.2024.04.002","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T11:05:38Z","timestamp":1714475138000},"page":"921-939.e17","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":62,"title":["Long-term expandable mouse and human-induced nephron progenitor cells enable kidney organoid maturation and modeling of plasticity and disease"],"prefix":"10.1016","volume":"31","author":[{"given":"Biao","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zipeng","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunghyun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connor C.","family":"Fausto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kari","family":"Koppitch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zexu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjin","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chennan C.","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyi","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Medina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan E.","family":"Schreiber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateo W.","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariel C.","family":"Vonk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyuan","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadrushi","family":"Patel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yidan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riana K.","family":"Parvez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balint","family":"Der","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyun Hao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenqing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew E.","family":"Thornton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan H.","family":"Grubbs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yarui","family":"Diao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yali","family":"Dou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ksenia","family":"Gnedeva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qilong","family":"Ying","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuria M.","family":"Pastor-Soler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teng","family":"Fei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth R.","family":"Hallows","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nils O.","family":"Lindstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew P.","family":"McMahon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongwei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.stem.2024.04.002_bib1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/bs.ctdb.2015.10.010","article-title":"Development of the mammalian kidney","volume":"117","author":"McMahon","year":"2016","journal-title":"Curr. Top. Dev. Biol."},{"key":"10.1016\/j.stem.2024.04.002_bib2","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1038\/s41581-022-00598-5","article-title":"Principles of human and mouse nephron development","volume":"18","author":"Schnell","year":"2022","journal-title":"Nat. Rev. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib3","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.stem.2008.05.020","article-title":"Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development","volume":"3","author":"Kobayashi","year":"2008","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib4","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1681\/ASN.2017050561","article-title":"Novel insights into the pathogenesis of monogenic congenital anomalies of the kidney and urinary tract","volume":"29","author":"van der Ven","year":"2018","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib5","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1038\/s41581-019-0112-0","article-title":"The genetic changes of Wilms tumour","volume":"15","author":"Treger","year":"2019","journal-title":"Nat. Rev. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib6","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.stem.2013.11.010","article-title":"Redefining the in\u00a0vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells","volume":"14","author":"Taguchi","year":"2014","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib7","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms9715","article-title":"Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids","volume":"6","author":"Freedman","year":"2015","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2024.04.002_bib8","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1038\/nbt.3392","article-title":"Nephron organoids derived from human pluripotent stem cells model kidney development and injury","volume":"33","author":"Morizane","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.stem.2024.04.002_bib9","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1038\/nature15695","article-title":"Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis","volume":"526","author":"Takasato","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.stem.2024.04.002_bib10","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.devcel.2015.06.021","article-title":"A synthetic niche for nephron progenitor cells","volume":"34","author":"Brown","year":"2015","journal-title":"Dev. Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib11","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/j.celrep.2016.03.076","article-title":"Selective in\u00a0vitro propagation of nephron progenitors derived from embryos and pluripotent stem cells","volume":"15","author":"Tanigawa","year":"2016","journal-title":"Cell Rep."},{"key":"10.1016\/j.stem.2024.04.002_bib12","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.stem.2016.07.016","article-title":"3D culture supports long-term expansion of mouse and human nephrogenic progenitors","volume":"19","author":"Li","year":"2016","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib13","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1101\/gad.329573.119","article-title":"Kidney organoids: accurate models or fortunate accidents","volume":"33","author":"Little","year":"2019","journal-title":"Genes Dev."},{"key":"10.1016\/j.stem.2024.04.002_bib14","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1016\/j.stem.2018.10.010","article-title":"Comparative analysis and refinement of human PSC-derived kidney organoid differentiation with single-cell transcriptomics","volume":"23","author":"Wu","year":"2018","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib15","doi-asserted-by":"crossref","DOI":"10.1186\/s13073-019-0615-0","article-title":"Single-cell analysis reveals congruence between kidney organoids and human fetal kidney","volume":"11","author":"Combes","year":"2019","journal-title":"Genome Med."},{"key":"10.1016\/j.stem.2024.04.002_bib16","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-022-33623-z","article-title":"Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids","volume":"13","author":"Vanslambrouck","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2024.04.002_bib17","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.1247005","article-title":"Genome-scale CRISPR-Cas9 knockout screening in human cells","volume":"343","author":"Shalem","year":"2014","journal-title":"Science"},{"key":"10.1016\/j.stem.2024.04.002_bib18","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1126\/science.1246981","article-title":"Genetic screens in human cells using the CRISPR-Cas9 system","volume":"343","author":"Wang","year":"2014","journal-title":"Science"},{"key":"10.1016\/j.stem.2024.04.002_bib19","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/nrg.2017.97","article-title":"Am I ready for CRISPR? A user's guide to genetic screens","volume":"19","author":"Doench","year":"2018","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.stem.2024.04.002_bib20","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.stemcr.2019.07.003","article-title":"Activin is superior to BMP7 for efficient maintenance of human iPSC-derived nephron progenitors","volume":"13","author":"Tanigawa","year":"2019","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.stem.2024.04.002_bib21","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/978-1-4939-9021-4_13","article-title":"Gene editing in 3D cultured nephron progenitor cell lines","volume":"1926","author":"Li","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.stem.2024.04.002_bib22","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-021-23911-5","article-title":"Generation of patterned kidney organoids that recapitulate the adult kidney collecting duct system from expandable ureteric bud progenitors","volume":"12","author":"Zeng","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2024.04.002_bib23","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/s41563-019-0287-6","article-title":"Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells","volume":"18","author":"Garreta","year":"2019","journal-title":"Nat. Mater."},{"key":"10.1016\/j.stem.2024.04.002_bib24","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1681\/ASN.2017080890","article-title":"Conserved and divergent features of mesenchymal progenitor cell types\u00a0within the cortical nephrogenic niche of the human and mouse kidney","volume":"29","author":"Lindstr\u00f6m","year":"2018","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib25","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.41156","article-title":"Nephron progenitor commitment is a stochastic process influenced by cell migration","volume":"8","author":"Lawlor","year":"2019","journal-title":"eLife"},{"key":"10.1016\/j.stem.2024.04.002_bib26","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1038\/nbt.3437","article-title":"Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9","volume":"34","author":"Doench","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.stem.2024.04.002_bib27","doi-asserted-by":"crossref","DOI":"10.1186\/s13059-015-0843-6","article-title":"Quality control, modeling, and visualization of CRISPR screens with MAGeCK-VISPR","volume":"16","author":"Li","year":"2015","journal-title":"Genome Biol."},{"key":"10.1016\/j.stem.2024.04.002_bib28","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1038\/s41596-018-0113-7","article-title":"Integrative analysis of pooled CRISPR genetic screens using MAGeCKFlute","volume":"14","author":"Wang","year":"2019","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.stem.2024.04.002_bib29","doi-asserted-by":"crossref","first-page":"1898","DOI":"10.1681\/ASN.2020091321","article-title":"Increasing mTORC1 pathway activity or methionine supplementation during pregnancy reverses the negative effect of maternal malnutrition on the developing kidney","volume":"32","author":"Makayes","year":"2021","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib30","doi-asserted-by":"crossref","first-page":"5998","DOI":"10.1073\/pnas.1712955115","article-title":"Hamartin regulates cessation of mouse nephrogenesis independently of Mtor","volume":"115","author":"Volovelsky","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2024.04.002_bib31","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1681\/ASN.2013040390","article-title":"MicroRNA-17\u223c92 is required for nephrogenesis and renal function","volume":"25","author":"Marrone","year":"2014","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib32","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1681\/ASN.2010080841","article-title":"The pro-apoptotic protein Bim is a microRNA target in kidney progenitors","volume":"22","author":"Ho","year":"2011","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib33","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1681\/ASN.2018111170","article-title":"Von Hippel-Lindau acts as a metabolic switch controlling nephron progenitor differentiation","volume":"30","author":"Cargill","year":"2019","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib34","doi-asserted-by":"crossref","first-page":"3105","DOI":"10.1242\/dev.128.16.3105","article-title":"Murine homolog of SALL1 is essential for ureteric bud invasion in kidney development","volume":"128","author":"Nishinakamura","year":"2001","journal-title":"Development"},{"key":"10.1016\/j.stem.2024.04.002_bib35","doi-asserted-by":"crossref","first-page":"2584","DOI":"10.1681\/ASN.2013080896","article-title":"Sall1 maintains nephron progenitors and nascent nephrons by acting as both an activator and a repressor","volume":"25","author":"Kanda","year":"2014","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib36","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1242\/dev.095851","article-title":"Sall1 balances self-renewal and differentiation of renal progenitor cells","volume":"141","author":"Basta","year":"2014","journal-title":"Development"},{"key":"10.1016\/j.stem.2024.04.002_bib37","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms5444","article-title":"WT1 controls antagonistic FGF and BMP-pSMAD pathways in early renal progenitors","volume":"5","author":"Motamedi","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2024.04.002_bib38","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.260356","article-title":"Foxc1 and Foxc2 are indispensable for the maintenance of nephron and stromal progenitors in the developing kidney","volume":"135","author":"Motojima","year":"2022","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.stem.2024.04.002_bib39","first-page":"4173","article-title":"Myc cooperates with \u03b2-catenin to drive gene expression in nephron progenitor cells","volume":"144","author":"Pan","year":"2017","journal-title":"Development"},{"key":"10.1016\/j.stem.2024.04.002_bib40","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.devcel.2014.10.015","article-title":"Eya1 interacts with Six2 and Myc to regulate expansion of the nephron progenitor pool during nephrogenesis","volume":"31","author":"Xu","year":"2014","journal-title":"Dev. Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib41","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1242\/dev.127175","article-title":"Differential regulation of mouse and human nephron progenitors by the Six family of transcriptional regulators","volume":"143","author":"O'Brien","year":"2016","journal-title":"Development"},{"key":"10.1016\/j.stem.2024.04.002_bib42","doi-asserted-by":"crossref","DOI":"10.1242\/dev.199684","article-title":"Sobp modulates the transcriptional activation of Six1 target genes and is required during craniofacial development","volume":"148","author":"Tavares","year":"2021","journal-title":"Development"},{"key":"10.1016\/j.stem.2024.04.002_bib43","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1002\/sctm.19-0289","article-title":"Epigenetic regulation of kidney progenitor cells","volume":"9","author":"Huang","year":"2020","journal-title":"Stem Cells Transl. Med."},{"key":"10.1016\/j.stem.2024.04.002_bib44","doi-asserted-by":"crossref","DOI":"10.1242\/dev.153619","article-title":"Histone deacetylases 1 and 2 regulate the transcriptional programs of nephron progenitors and renal vesicles","volume":"145","author":"Liu","year":"2018","journal-title":"Development"},{"key":"10.1016\/j.stem.2024.04.002_bib45","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.ydbio.2012.11.018","article-title":"Mi-2\/NuRD is required in renal progenitor cells during embryonic kidney development","volume":"375","author":"Denner","year":"2013","journal-title":"Dev. Biol."},{"key":"10.1016\/j.stem.2024.04.002_bib46","doi-asserted-by":"crossref","first-page":"11542","DOI":"10.1074\/jbc.RA120.013348","article-title":"The polycomb proteins EZH1 and EZH2 co-regulate chromatin accessibility and nephron progenitor cell lifespan in mice","volume":"295","author":"Liu","year":"2020","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.stem.2024.04.002_bib47","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.ydbio.2020.05.008","article-title":"The core SWI\/SNF catalytic subunit Brg1 regulates nephron progenitor cell proliferation and differentiation","volume":"464","author":"Basta","year":"2020","journal-title":"Dev. Biol."},{"key":"10.1016\/j.stem.2024.04.002_bib48","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.abj1578","article-title":"A selective WDR5 degrader inhibits acute myeloid leukemia in patient-derived mouse models","volume":"13","author":"Yu","year":"2021","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.stem.2024.04.002_bib49","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1681\/ASN.2017121265","article-title":"Whole-exome sequencing identifies causative mutations in families with congenital anomalies of the kidney and urinary tract","volume":"29","author":"van der Ven","year":"2018","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib50","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1038\/ncb2828","article-title":"Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation","volume":"15","author":"Das","year":"2013","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.stem.2024.04.002_bib51","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-021-23395-3","article-title":"Small-molecule inhibition of Lats kinases may promote Yap-dependent proliferation in postmitotic mammalian tissues","volume":"12","author":"Kastan","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2024.04.002_bib52","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-019-13382-0","article-title":"Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation","volume":"10","author":"Subramanian","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2024.04.002_bib53","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/s41592-018-0253-2","article-title":"Evaluation of variability in human kidney organoids","volume":"16","author":"Phipson","year":"2019","journal-title":"Nat. Methods"},{"key":"10.1016\/j.stem.2024.04.002_bib54","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2219699120","article-title":"A single-cell multiomic analysis of kidney organoid differentiation","volume":"120","author":"Yoshimura","year":"2023","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2024.04.002_bib55","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1038\/nrneph.2012.259","article-title":"Alport syndrome--insights from basic and clinical research","volume":"9","author":"Kruegel","year":"2013","journal-title":"Nat. Rev. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib56","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.devcel.2019.06.001","article-title":"In\u00a0vivo developmental trajectories of human podocyte inform in\u00a0vitro differentiation of pluripotent stem cell-derived podocytes","volume":"50","author":"Tran","year":"2019","journal-title":"Dev. Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib57","doi-asserted-by":"crossref","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","article-title":"Integrated analysis of multimodal single-cell data","volume":"184","author":"Hao","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib58","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-021-22368-w","article-title":"Single cell transcriptional and chromatin accessibility profiling redefine cellular heterogeneity in the adult human kidney","volume":"12","author":"Muto","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2024.04.002_bib59","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/j.devcel.2018.05.010","article-title":"Progressive recruitment of mesenchymal progenitors reveals a time-dependent process of cell fate acquisition in mouse and human nephrogenesis","volume":"45","author":"Lindstr\u00f6m","year":"2018","journal-title":"Dev. Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib60","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1016\/j.stem.2022.06.005","article-title":"A scalable organoid model of human autosomal dominant polycystic kidney disease for disease mechanism and drug discovery","volume":"29","author":"Tran","year":"2022","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib61","doi-asserted-by":"crossref","DOI":"10.1038\/s41572-018-0047-y","article-title":"Polycystic kidney disease","volume":"4","author":"Bergmann","year":"2018","journal-title":"Nat. Rev. Dis. Primers"},{"key":"10.1016\/j.stem.2024.04.002_bib62","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1038\/nmat4994","article-title":"Organoid cystogenesis reveals a critical role of microenvironment in human polycystic kidney disease","volume":"16","author":"Cruz","year":"2017","journal-title":"Nat. Mater."},{"key":"10.1016\/j.stem.2024.04.002_bib63","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/j.stem.2018.04.022","article-title":"High-throughput screening enhances kidney organoid differentiation from human pluripotent stem cells and enables automated multidimensional phenotyping","volume":"22","author":"Czerniecki","year":"2018","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib64","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1681\/ASN.2020030378","article-title":"PKD1-dependent renal cystogenesis in human induced pluripotent stem cell-derived ureteric bud\/collecting duct organoids","volume":"31","author":"Kuraoka","year":"2020","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib65","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1016\/j.bbrc.2020.06.141","article-title":"A novel ADPKD model using kidney organoids derived from disease-specific human iPSCs","volume":"529","author":"Shimizu","year":"2020","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.stem.2024.04.002_bib66","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1038\/cr.2017.81","article-title":"One-step generation of complete gene knockout mice and monkeys by CRISPR\/Cas9-mediated gene editing with multiple sgRNAs","volume":"27","author":"Zuo","year":"2017","journal-title":"Cell Res."},{"key":"10.1016\/j.stem.2024.04.002_bib67","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1681\/ASN.2007070828","article-title":"Small-molecule CFTR inhibitors slow cyst growth in polycystic kidney disease","volume":"19","author":"Yang","year":"2008","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib68","doi-asserted-by":"crossref","first-page":"2462","DOI":"10.1073\/pnas.1011498108","article-title":"Activating AMP-activated protein kinase (AMPK) slows renal cystogenesis","volume":"108","author":"Takiar","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2024.04.002_bib69","doi-asserted-by":"crossref","first-page":"F27","DOI":"10.1152\/ajprenal.00298.2021","article-title":"Metformin improves relevant disease parameters in an autosomal dominant polycystic kidney disease mouse model","volume":"322","author":"Pastor-Soler","year":"2022","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"10.1016\/j.stem.2024.04.002_bib70","doi-asserted-by":"crossref","first-page":"2751","DOI":"10.1172\/JCI90921","article-title":"Lysine methyltransferase SMYD2 promotes cyst growth in autosomal dominant polycystic kidney disease","volume":"127","author":"Li","year":"2017","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.stem.2024.04.002_bib71","doi-asserted-by":"crossref","first-page":"F227","DOI":"10.1152\/ajprenal.00452.2021","article-title":"Cross talk between lysine methyltransferase Smyd2 and TGF-\u03b2-Smad3 signaling promotes renal fibrosis in autosomal dominant polycystic kidney disease","volume":"323","author":"Li","year":"2022","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"10.1016\/j.stem.2024.04.002_bib72","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.1056\/NEJMoa1205511","article-title":"Tolvaptan in patients with autosomal dominant polycystic kidney disease","volume":"367","author":"Torres","year":"2012","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.stem.2024.04.002_bib73","doi-asserted-by":"crossref","first-page":"F1005","DOI":"10.1152\/ajprenal.00243.2011","article-title":"Tolvaptan inhibits ERK-dependent cell proliferation, Cl\u207b secretion, and in\u00a0vitro cyst growth of human ADPKD cells stimulated by vasopressin","volume":"301","author":"Reif","year":"2011","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"10.1016\/j.stem.2024.04.002_bib74","doi-asserted-by":"crossref","first-page":"5466","DOI":"10.1073\/pnas.0509694103","article-title":"The mTOR pathway is regulated by polycystin-1, and its inhibition reverses renal cystogenesis in polycystic kidney disease","volume":"103","author":"Shillingford","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2024.04.002_bib75","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1681\/ASN.2004080660","article-title":"Rapamycin markedly slows disease progression in a rat model of polycystic kidney disease","volume":"16","author":"Tao","year":"2005","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib76","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1101\/gad.315127.118","article-title":"A RhoA-YAP-c-Myc signaling axis promotes the development of polycystic kidney disease","volume":"32","author":"Cai","year":"2018","journal-title":"Genes Dev."},{"key":"10.1016\/j.stem.2024.04.002_bib77","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1038\/ki.1991.80","article-title":"C-myc as an inducer of polycystic kidney disease in transgenic mice","volume":"39","author":"Trudel","year":"1991","journal-title":"Kidney Int."},{"key":"10.1016\/j.stem.2024.04.002_bib78","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1038\/s41581-018-0051-1","article-title":"Metabolism and mitochondria in polycystic kidney disease research and therapy","volume":"14","author":"Padovano","year":"2018","journal-title":"Nat. Rev. Nephrol."},{"key":"10.1016\/j.stem.2024.04.002_bib79","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1038\/nm.3092","article-title":"Defective glucose metabolism in polycystic kidney disease identifies a new therapeutic strategy","volume":"19","author":"Rowe","year":"2013","journal-title":"Nat. Med."},{"key":"10.1016\/j.stem.2024.04.002_bib80","doi-asserted-by":"crossref","first-page":"21819","DOI":"10.1073\/pnas.0911987106","article-title":"Chemical modifier screen identifies HDAC inhibitors as suppressors of PKD models","volume":"106","author":"Cao","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2024.04.002_bib81","doi-asserted-by":"crossref","first-page":"3982","DOI":"10.1093\/hmg\/ddv136","article-title":"Therapeutic targeting of BET bromodomain protein, Brd4, delays cyst growth in ADPKD","volume":"24","author":"Zhou","year":"2015","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.stem.2024.04.002_bib82","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.kint.2016.01.026","article-title":"Inhibition of histone deacetylase 6 activity reduces cyst growth in polycystic kidney disease","volume":"90","author":"Cebotaru","year":"2016","journal-title":"Kidney Int."},{"key":"10.1016\/j.stem.2024.04.002_bib83","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1016\/j.stemcr.2018.01.008","article-title":"Generation of functioning nephrons by implanting human pluripotent stem cell-derived kidney progenitors","volume":"10","author":"Bantounas","year":"2018","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.stem.2024.04.002_bib84","doi-asserted-by":"crossref","DOI":"10.1038\/s41536-022-00237-4","article-title":"Vasculogenesis in kidney organoids upon transplantation","volume":"7","author":"Koning","year":"2022","journal-title":"NPJ Regen. Med."},{"key":"10.1016\/j.stem.2024.04.002_bib85","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1126\/science.aar2131","article-title":"Single-cell transcriptomics of the mouse kidney reveals potential cellular targets of kidney disease","volume":"360","author":"Park","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.stem.2024.04.002_bib86","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/nmeth.3047","article-title":"Improved vectors and genome-wide libraries for CRISPR screening","volume":"11","author":"Sanjana","year":"2014","journal-title":"Nat. Methods"},{"key":"10.1016\/j.stem.2024.04.002_bib87","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1016\/j.cell.2006.01.040","article-title":"A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen","volume":"124","author":"Moffat","year":"2006","journal-title":"Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib88","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/j.cels.2018.10.008","article-title":"Mapping cellular reprogramming via pooled overexpression screens with paired fitness and single-cell RNA-sequencing readout","volume":"7","author":"Parekh","year":"2018","journal-title":"Cell Syst."},{"key":"10.1016\/j.stem.2024.04.002_bib89","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.ymeth.2015.12.012","article-title":"Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives","volume":"101","author":"Oceguera-Yanez","year":"2016","journal-title":"Methods"},{"key":"10.1016\/j.stem.2024.04.002_bib90","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.stem.2013.03.006","article-title":"Enhanced efficiency of human pluripotent stem cell genome editing through replacing TALENs with CRISPRs","volume":"12","author":"Ding","year":"2013","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib91","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.stem.2013.04.019","article-title":"Single-cell analysis reveals that expression of nanog is biallelic and equally variable as that of other pluripotency factors in mouse ESCs","volume":"13","author":"Faddah","year":"2013","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib92","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1038\/nprot.2013.143","article-title":"Genome engineering using the CRISPR-Cas9 system","volume":"8","author":"Ran","year":"2013","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.stem.2024.04.002_bib93","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.stem.2024.04.002_bib94","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.stem.2019.06.009","article-title":"Generation of human PSC-derived kidney organoids with patterned nephron segments and a de novo vascular network","volume":"25","author":"Low","year":"2019","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2024.04.002_bib95","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.isci.2019.10.041","article-title":"KDM3A and KDM4C regulate mesenchymal stromal cell senescence and bone aging via condensin-mediated heterochromatin reorganization","volume":"21","author":"Huang","year":"2019","journal-title":"Iscience"}],"container-title":["Cell Stem Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1934590924001292?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1934590924001292?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T00:30:59Z","timestamp":1762561859000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1934590924001292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":95,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["S1934590924001292"],"URL":"https:\/\/doi.org\/10.1016\/j.stem.2024.04.002","relation":{},"ISSN":["1934-5909"],"issn-type":[{"value":"1934-5909","type":"print"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Long-term expandable mouse and human-induced nephron progenitor cells enable kidney organoid maturation and modeling of plasticity and disease","name":"articletitle","label":"Article Title"},{"value":"Cell Stem Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.stem.2024.04.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier Inc.","name":"copyright","label":"Copyright"}]}}