{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:43:35Z","timestamp":1791974615657,"version":"4.4.0"},"reference-count":108,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003452","name":"Innovation and Technology Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003452","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003802","name":"University Research Committee, University of Hong Kong","doi-asserted-by":"publisher","award":["T12-710\/16-R"],"award-info":[{"award-number":["T12-710\/16-R"]}],"id":[{"id":"10.13039\/501100003802","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002920","name":"Research Grants Council, University Grants Committee","doi-asserted-by":"publisher","award":["YCRG-C7004-22Y"],"award-info":[{"award-number":["YCRG-C7004-22Y"]}],"id":[{"id":"10.13039\/501100002920","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002920","name":"Research Grants Council, University Grants Committee","doi-asserted-by":"publisher","award":["109002147"],"award-info":[{"award-number":["109002147"]}],"id":[{"id":"10.13039\/501100002920","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003803","name":"University of Hong Kong","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003803","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Stem Cell"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.stem.2025.08.006","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T14:41:40Z","timestamp":1756824100000},"page":"1595-1613.e10","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"title":["A combined enteric neuron-gastric tumor organoid reveals metabolic vulnerabilities in gastric cancer"],"prefix":"10.1016","volume":"32","author":[{"given":"Becky K.C.","family":"Chan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7275-0351","authenticated-orcid":false,"given":"Chi Him","family":"Poon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie H.Y.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoi Yee","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sin-Guang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5693-8231","authenticated-orcid":false,"given":"Helen H.N.","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8614-4619","authenticated-orcid":false,"given":"Suet Yi","family":"Leung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1790-3233","authenticated-orcid":false,"given":"Alan S.L.","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.stem.2025.08.006_bib1","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.ccell.2023.02.016","article-title":"The evolving tumor microenvironment: From cancer initiation to metastatic outgrowth","volume":"41","author":"de Visser","year":"2023","journal-title":"Cancer Cell"},{"key":"10.1016\/j.stem.2025.08.006_bib2","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/s41568-019-0237-2","article-title":"Nerves in cancer","volume":"20","author":"Zahalka","year":"2020","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.stem.2025.08.006_bib3","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1038\/s41575-022-00669-9","article-title":"Nerves in gastrointestinal cancer: from mechanism to modulations","volume":"19","author":"Vaes","year":"2022","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"10.1016\/j.stem.2025.08.006_bib4","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1146\/annurev-cancerbio-030518-055702","article-title":"Organoid Models for Cancer Research","volume":"3","author":"Clevers","year":"2019","journal-title":"Annu. Rev. Cancer Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib5","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1038\/s41568-024-00706-6","article-title":"Cancer organoids 2.0: modelling the complexity of the tumour immune microenvironment","volume":"24","author":"Polak","year":"2024","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.stem.2025.08.006_bib6","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1038\/s41586-019-1103-9","article-title":"Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens","volume":"568","author":"Behan","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.stem.2025.08.006_bib7","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.ccell.2023.12.016","article-title":"A comprehensive clinically informed map of dependencies in cancer cells and framework for target prioritization","volume":"42","author":"Pacini","year":"2024","journal-title":"Cancer Cell"},{"key":"10.1016\/j.stem.2025.08.006_bib8","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1016\/j.stem.2020.04.003","article-title":"Pooled In Vitro and In Vivo CRISPR-Cas9 Screening Identifies Tumor Suppressors in Human Colon Organoids","volume":"26","author":"Michels","year":"2020","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2025.08.006_bib9","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.stem.2020.02.007","article-title":"Genome-Scale CRISPR Screening in Human Intestinal Organoids Identifies Drivers of TGF-beta Resistance","volume":"26","author":"Ringel","year":"2020","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2025.08.006_bib10","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2016806118","article-title":"A genome-scale CRISPR screen reveals factors regulating Wnt-dependent renewal of mouse gastric epithelial cells","volume":"118","author":"Murakami","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2025.08.006_bib11","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.stem.2021.11.001","article-title":"Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis","volume":"29","author":"Ungricht","year":"2022","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2025.08.006_bib12","doi-asserted-by":"crossref","DOI":"10.1002\/smtd.202201605","article-title":"CRISPR\/Cas9 Screen in Gastric Cancer Patient-Derived Organoids Reveals KDM1A-NDRG1 Axis as a Targetable Vulnerability","volume":"7","author":"Mircetic","year":"2023","journal-title":"Small Methods"},{"key":"10.1016\/j.stem.2025.08.006_bib13","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adg4055","article-title":"An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate","volume":"9","author":"Hansen","year":"2023","journal-title":"Sci. Adv."},{"key":"10.1016\/j.stem.2025.08.006_bib14","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1146\/annurev-bioeng-103023-122327","article-title":"Human Organoids as an Emerging Tool for Genome Screenings","volume":"27","author":"Andreatta","year":"2025","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"10.1016\/j.stem.2025.08.006_bib15","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1016\/j.stem.2018.09.016","article-title":"A Comprehensive Human Gastric Cancer Organoid Biobank Captures Tumor Subtype Heterogeneity and Enables Therapeutic Screening","volume":"23","author":"Yan","year":"2018","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2025.08.006_bib16","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1136\/gutjnl-2022-327121","article-title":"Escape from cell-cell and cell-matrix adhesion dependence underscores disease progression in gastric cancer organoid models","volume":"72","author":"Tong","year":"2023","journal-title":"Gut"},{"key":"10.1016\/j.stem.2025.08.006_bib19","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1038\/nbt.3437","article-title":"Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9","volume":"34","author":"Doench","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.stem.2025.08.006_bib20","doi-asserted-by":"crossref","DOI":"10.1186\/s13059-014-0554-4","article-title":"MAGeCK enables robust identification of essential genes from genome-scale CRISPR\/Cas9 knockout screens","volume":"15","author":"Li","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib21","doi-asserted-by":"crossref","DOI":"10.1186\/s13073-020-00809-3","article-title":"Improved analysis of CRISPR fitness screens and reduced off-target effects with the BAGEL2 gene essentiality classifier","volume":"13","author":"Kim","year":"2021","journal-title":"Genome Med."},{"key":"10.1016\/j.stem.2025.08.006_bib17","doi-asserted-by":"crossref","DOI":"10.15252\/msb.20145216","article-title":"Measuring error rates in genomic perturbation screens: gold standards for human functional genomics","volume":"10","author":"Hart","year":"2014","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib18","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1534\/g3.117.041277","article-title":"Evaluation and Design of Genome-Wide CRISPR\/SpCas9 Knockout Screens","volume":"7","author":"Hart","year":"2017","journal-title":"G3 (Bethesda)"},{"key":"10.1016\/j.stem.2025.08.006_bib22","doi-asserted-by":"crossref","DOI":"10.1186\/s13059-021-02540-7","article-title":"Chronos: a cell population dynamics model of CRISPR experiments that improves inference of gene fitness effects","volume":"22","author":"Dempster","year":"2021","journal-title":"Genome Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib23","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1038\/nm.4399","article-title":"Targeting mitochondrial oxidative phosphorylation eradicates therapy-resistant chronic myeloid leukemia stem cells","volume":"23","author":"Kuntz","year":"2017","journal-title":"Nat. Med."},{"key":"10.1016\/j.stem.2025.08.006_bib24","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1038\/bjc.2016.152","article-title":"Hallmarks of cancer stem cell metabolism","volume":"114","author":"Sancho","year":"2016","journal-title":"Br. J. Cancer"},{"key":"10.1016\/j.stem.2025.08.006_bib25","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1039\/C7MD00633K","article-title":"Approaches to target tractability assessment - a practical perspective","volume":"9","author":"Brown","year":"2018","journal-title":"MedChemComm"},{"key":"10.1016\/j.stem.2025.08.006_bib26","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/s41573-021-00245-x","article-title":"The PROTACtable genome","volume":"20","author":"Schneider","year":"2021","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.stem.2025.08.006_bib27","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.4161\/rna.25827","article-title":"Two mammalian MAGOH genes contribute to exon junction complex composition and nonsense-mediated decay","volume":"10","author":"Singh","year":"2013","journal-title":"RNA Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib28","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1080\/15476286.2023.2221511","article-title":"The paralogues MAGOH and MAGOHB are oncogenic factors in high-grade gliomas and safeguard the splicing of cell division and cell cycle genes","volume":"20","author":"Barreiro","year":"2023","journal-title":"RNA Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib29","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1038\/s41591-018-0052-4","article-title":"An inhibitor of oxidative phosphorylation exploits cancer vulnerability","volume":"24","author":"Molina","year":"2018","journal-title":"Nat. Med."},{"key":"10.1016\/j.stem.2025.08.006_bib30","doi-asserted-by":"crossref","first-page":"E1796","DOI":"10.1073\/pnas.1520686113","article-title":"Acetyl-CoA carboxylase inhibition by ND-630 reduces hepatic steatosis, improves insulin sensitivity, and modulates dyslipidemia in rats","volume":"113","author":"Harriman","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2025.08.006_bib31","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1038\/nm.4181","article-title":"Inhibition of acetyl-CoA carboxylase suppresses fatty acid synthesis and tumor growth of non-small-cell lung cancer in preclinical models","volume":"22","author":"Svensson","year":"2016","journal-title":"Nat. Med."},{"key":"10.1016\/j.stem.2025.08.006_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.eclinm.2021.100797","article-title":"First-in-human study of the safety, pharmacokinetics, and pharmacodynamics of first-in-class fatty acid synthase inhibitor TVB-2640 alone and with a taxane in advanced tumors","volume":"34","author":"Falchook","year":"2021","journal-title":"EClinicalmedicine"},{"key":"10.1016\/j.stem.2025.08.006_bib33","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.ccell.2020.12.008","article-title":"Targeting pan-essential genes in cancer: Challenges and opportunities","volume":"39","author":"Chang","year":"2021","journal-title":"Cancer Cell"},{"key":"10.1016\/j.stem.2025.08.006_bib34","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1038\/s41586-020-2099-x","article-title":"CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities","volume":"580","author":"Han","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.stem.2025.08.006_bib35","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1038\/s41580-020-0259-3","article-title":"Human organoids: model systems for human biology and medicine","volume":"21","author":"Kim","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib36","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/s41568-018-0007-6","article-title":"Organoids in cancer research","volume":"18","author":"Drost","year":"2018","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.stem.2025.08.006_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.plipres.2019.101017","article-title":"The highly unnatural fatty acid profile of cells in culture","volume":"77","author":"Else","year":"2020","journal-title":"Prog. Lipid Res."},{"key":"10.1016\/j.stem.2025.08.006_bib38","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.lfs.2018.04.049","article-title":"Omega-3 and omega-6 polyunsaturated fatty acids: Dietary sources, metabolism, and significance - A review","volume":"203","author":"Saini","year":"2018","journal-title":"Life Sci."},{"key":"10.1016\/j.stem.2025.08.006_bib39","doi-asserted-by":"crossref","first-page":"2596","DOI":"10.1016\/j.celrep.2018.08.015","article-title":"Triglycerides Promote Lipid Homeostasis during Hypoxic Stress by Balancing Fatty Acid Saturation","volume":"24","author":"Ackerman","year":"2018","journal-title":"Cell Rep."},{"key":"10.1016\/j.stem.2025.08.006_bib40","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1038\/s42255-023-00769-z","article-title":"Triglyceride cycling enables modification of stored fatty acids","volume":"5","author":"Wunderling","year":"2023","journal-title":"Nat. Metab."},{"key":"10.1016\/j.stem.2025.08.006_bib41","doi-asserted-by":"crossref","DOI":"10.1172\/JCI143776","article-title":"Exploiting unique features of the gut-brain interface to combat gastrointestinal cancer","volume":"131","author":"Schledwitz","year":"2021","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.stem.2025.08.006_bib42","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.jcmgh.2017.10.002","article-title":"Colorectal Cancer Cells Adhere to and Migrate Along the Neurons of the Enteric Nervous System","volume":"5","author":"Duchalais","year":"2018","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"10.1016\/j.stem.2025.08.006_bib43","doi-asserted-by":"crossref","first-page":"3868","DOI":"10.1158\/0008-5472.CAN-23-1012","article-title":"Neurotransmitter Receptor HTR2B Regulates Lipid Metabolism to Inhibit Ferroptosis in Gastric Cancer","volume":"83","author":"Tu","year":"2023","journal-title":"Cancer Res."},{"key":"10.1016\/j.stem.2025.08.006_bib44","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2021.102091","article-title":"Neural signaling modulates metabolism of gastric cancer","volume":"24","author":"Rabben","year":"2021","journal-title":"iScience"},{"key":"10.1016\/j.stem.2025.08.006_bib45","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.61080","article-title":"A versatile system to record cell-cell interactions","volume":"9","author":"Tang","year":"2020","journal-title":"Elife"},{"key":"10.1016\/j.stem.2025.08.006_bib46","doi-asserted-by":"crossref","first-page":"7957","DOI":"10.1073\/pnas.1820989116","article-title":"Target identification reveals lanosterol synthase as a vulnerability in glioma","volume":"116","author":"Phillips","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2025.08.006_bib47","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.chembiol.2023.01.005","article-title":"Selective and brain-penetrant lanosterol synthase inhibitors target glioma stem-like cells by inducing 24(S),25-epoxycholesterol production","volume":"30","author":"Nguyen","year":"2023","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib48","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1016\/j.chembiol.2017.08.026","article-title":"Very Long Chain Fatty Acids Are Functionally Involved in Necroptosis","volume":"24","author":"Parisi","year":"2017","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib49","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1039\/D2CB00172A","article-title":"SREBP activation contributes to fatty acid accumulations in necroptosis","volume":"4","author":"Lu","year":"2023","journal-title":"RSC Chem. Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib50","first-page":"2286","article-title":"Membrane Disruption by Very Long Chain Fatty Acids during Necroptosis","volume":"14","author":"Parisi","year":"2019","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib51","doi-asserted-by":"crossref","first-page":"10512","DOI":"10.1021\/jm5016022","article-title":"Decreasing the rate of metabolic ketone reduction in the discovery of a clinical acetyl-CoA carboxylase inhibitor for the treatment of diabetes","volume":"57","author":"Griffith","year":"2014","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.stem.2025.08.006_bib52","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1038\/s41591-021-01489-1","article-title":"ACC inhibitor alone or co-administered with a DGAT2 inhibitor in patients with non-alcoholic fatty liver disease: two parallel, placebo-controlled, randomized phase 2a trials","volume":"27","author":"Calle","year":"2021","journal-title":"Nat. Med."},{"key":"10.1016\/j.stem.2025.08.006_bib53","doi-asserted-by":"crossref","first-page":"10879","DOI":"10.1021\/acs.jmedchem.0c00640","article-title":"Optimizing the Benefit\/Risk of Acetyl-CoA Carboxylase Inhibitors through Liver Targeting","volume":"63","author":"Huard","year":"2020","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.stem.2025.08.006_bib54","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1038\/s42255-020-00272-9","article-title":"De novo lipogenesis is essential for platelet production in humans","volume":"2","author":"Kelly","year":"2020","journal-title":"Nat. Metab."},{"key":"10.1016\/j.stem.2025.08.006_bib55","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.cmet.2018.08.020","article-title":"Inhibition of Acetyl-CoA Carboxylase by Phosphorylation or the Inhibitor ND-654 Suppresses Lipogenesis and Hepatocellular Carcinoma","volume":"29","author":"Lally","year":"2019","journal-title":"Cell Metab."},{"key":"10.1016\/j.stem.2025.08.006_bib56","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1023\/A:1015870314340","article-title":"The bilayer floating capsule: a stomach-directed drug delivery system for misoprostol","volume":"9","author":"Oth","year":"1992","journal-title":"Pharm. Res."},{"key":"10.1016\/j.stem.2025.08.006_bib57","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/S0022-2275(20)37449-6","article-title":"Ro 48-8.071, a new 2,3-oxidosqualene:lanosterol cyclase inhibitor lowering plasma cholesterol in hamsters, squirrel monkeys, and minipigs: comparison to simvastatin","volume":"38","author":"Morand","year":"1997","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.stem.2025.08.006_bib58","doi-asserted-by":"crossref","first-page":"27","DOI":"10.4103\/0976-0105.177703","article-title":"A simple practice guide for dose conversion between animals and human","volume":"7","author":"Nair","year":"2016","journal-title":"J. Basic Clin. Pharm."},{"key":"10.1016\/j.stem.2025.08.006_bib59","doi-asserted-by":"crossref","DOI":"10.1038\/s43586-022-00174-y","article-title":"Organoids","volume":"2","author":"Zhao","year":"2022","journal-title":"Nat. Rev. Methods Primers"},{"key":"10.1016\/j.stem.2025.08.006_bib60","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1016\/j.devcel.2021.04.013","article-title":"Lipid metabolism in cancer: New perspectives and emerging mechanisms","volume":"56","author":"Broadfield","year":"2021","journal-title":"Dev. Cell"},{"key":"10.1016\/j.stem.2025.08.006_bib61","article-title":"HIF2alpha Promotes Cancer Metastasis through TCF7L2-Dependent Fatty Acid Synthesis in ccRCC","volume":"7","author":"Shi","year":"2024","journal-title":"Research (Wash D. C)"},{"key":"10.1016\/j.stem.2025.08.006_bib62","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.cmet.2017.10.005","article-title":"Advances in Hypoxia-Inducible Factor Biology","volume":"27","author":"Choudhry","year":"2018","journal-title":"Cell Metab."},{"key":"10.1016\/j.stem.2025.08.006_bib63","doi-asserted-by":"crossref","DOI":"10.1016\/j.metop.2022.100177","article-title":"Acute intermittent hypoxia drives hepatic de novo lipogenesis in humans and rodents","volume":"14","author":"Hazlehurst","year":"2022","journal-title":"Metabol. Open"},{"key":"10.1016\/j.stem.2025.08.006_bib64","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1053\/j.gastro.2024.01.027","article-title":"Oncogenic Fatty Acid Metabolism Rewires Energy Supply Chain in Gastric Carcinogenesis","volume":"166","author":"Won","year":"2024","journal-title":"Gastroenterology"},{"key":"10.1016\/j.stem.2025.08.006_bib65","article-title":"Engineered Hydrogels for Organoid Models of Human Nonalcoholic Fatty Liver Disease","volume":"12","author":"Liu","year":"2025","journal-title":"Adv. Sci. (Weinh)"},{"key":"10.1016\/j.stem.2025.08.006_bib66","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1038\/d41586-021-00182-0","article-title":"Cancer research needs a better map","volume":"589","author":"Boehm","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.stem.2025.08.006_bib67","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-022-09508-y","article-title":"A suspension technique for efficient large-scale cancer organoid culturing and perturbation screens","volume":"12","author":"Price","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.stem.2025.08.006_bib68","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.cell.2015.03.053","article-title":"Prospective derivation of a living organoid biobank of colorectal cancer patients","volume":"161","author":"van de Wetering","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.stem.2025.08.006_bib69","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.1136\/gutjnl-2019-320019","article-title":"Organoid cultures of early-onset colorectal cancers reveal distinct and rare genetic profiles","volume":"69","author":"Yan","year":"2020","journal-title":"Gut"},{"key":"10.1016\/j.stem.2025.08.006_bib70","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/s43018-022-00337-6","article-title":"A human breast cancer-derived xenograft and organoid platform for drug discovery and precision oncology","volume":"3","author":"Guillen","year":"2022","journal-title":"Nat. Cancer"},{"key":"10.1016\/j.stem.2025.08.006_bib71","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.cell.2017.11.010","article-title":"A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity","volume":"172","author":"Sachs","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.stem.2025.08.006_bib72","doi-asserted-by":"crossref","DOI":"10.1186\/s13059-021-02268-4","article-title":"Minimal genome-wide human CRISPR-Cas9 library","volume":"22","author":"Gon\u00e7alves","year":"2021","journal-title":"Genome Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib73","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/s44222-022-00013-5","article-title":"CRISPR engineering in organoids for gene repair and disease modelling","volume":"1","author":"Geurts","year":"2023","journal-title":"Nat. Rev. Bioeng."},{"key":"10.1016\/j.stem.2025.08.006_bib74","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1073\/pnas.1517883113","article-title":"Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM","volume":"113","author":"Wong","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2025.08.006_bib75","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2020.108020","article-title":"A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets","volume":"32","author":"Zhou","year":"2020","journal-title":"Cell Rep."},{"key":"10.1016\/j.stem.2025.08.006_bib76","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1038\/nbt.3834","article-title":"Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions","volume":"35","author":"Han","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.stem.2025.08.006_bib77","doi-asserted-by":"crossref","first-page":"6219","DOI":"10.1158\/0008-5472.CAN-21-1017","article-title":"A Combinatorial CRISPR-Cas9 Screen Identifies Ifenprodil as an Adjunct to Sorafenib for Liver Cancer Treatment","volume":"81","author":"Xu","year":"2021","journal-title":"Cancer Res."},{"key":"10.1016\/j.stem.2025.08.006_bib78","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1038\/s41586-025-09176-8","article-title":"Nerve-to-cancer transfer of mitochondria during cancer metastasis","volume":"644","author":"Hoover","year":"2025","journal-title":"Nature"},{"key":"10.1016\/j.stem.2025.08.006_bib103","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1186\/s12859-016-1015-8","article-title":"BAGEL: a computational framework for identifying essential genes from pooled library screens","volume":"17","author":"Hart","year":"2016","journal-title":"BMC Bioinform."},{"key":"10.1016\/j.stem.2025.08.006_bib79","doi-asserted-by":"crossref","DOI":"10.1186\/s12864-018-4989-y","article-title":"Unsupervised correction of gene-independent cell responses to CRISPR-Cas9 targeting","volume":"19","author":"Iorio","year":"2018","journal-title":"BMC Genomics"},{"key":"10.1016\/j.stem.2025.08.006_bib80","doi-asserted-by":"crossref","DOI":"10.1186\/s12864-021-08129-5","article-title":"CoRe: a robustly benchmarked R package for identifying core-fitness genes in genome-wide pooled CRISPR-Cas9 screens","volume":"22","author":"Vinceti","year":"2021","journal-title":"BMC Genomics"},{"key":"10.1016\/j.stem.2025.08.006_bib81","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1101\/gr.238923.118","article-title":"JACKS: joint analysis of CRISPR\/Cas9 knockout screens","volume":"29","author":"Allen","year":"2019","journal-title":"Genome Res."},{"key":"10.1016\/j.stem.2025.08.006_bib82","doi-asserted-by":"crossref","DOI":"10.1186\/s13073-019-0665-3","article-title":"Identifying chemogenetic interactions from CRISPR screens with drugZ","volume":"11","author":"Colic","year":"2019","journal-title":"Genome Med."},{"key":"10.1016\/j.stem.2025.08.006_bib104","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1093\/bioinformatics\/bts034","article-title":"The sva package for removing batch effects and other unwanted variation in high-throughput experiments","volume":"28","author":"Leek","year":"2012","journal-title":"Bioinform."},{"key":"10.1016\/j.stem.2025.08.006_bib83","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","article-title":"ShinyGO: a graphical gene-set enrichment tool for animals and plants","volume":"36","author":"Ge","year":"2020","journal-title":"Bioinform."},{"key":"10.1016\/j.stem.2025.08.006_bib105","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkz114","article-title":"The R package Rsubread is easier, faster, cheaper and better for alignment and quantification of RNA sequencing reads","volume":"47","author":"Liao","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.stem.2025.08.006_bib106","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinform."},{"key":"10.1016\/j.stem.2025.08.006_bib107","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"Ritchie","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.stem.2025.08.006_bib102","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinform."},{"key":"10.1016\/j.stem.2025.08.006_bib84","doi-asserted-by":"crossref","DOI":"10.1186\/s13059-019-1842-9","article-title":"Accuracy assessment of fusion transcript detection via read-mapping and de novo fusion transcript assembly-based methods","volume":"20","author":"Haas","year":"2019","journal-title":"Genome Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib85","doi-asserted-by":"crossref","first-page":"E5593","DOI":"10.1073\/pnas.1419161111","article-title":"rMATS: robust and flexible detection of differential alternative splicing from replicate RNA-Seq data","volume":"111","author":"Shen","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2025.08.006_bib108","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.stem.2025.08.006_bib86","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1038\/s41592-019-0473-0","article-title":"Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9","volume":"16","author":"Choi","year":"2019","journal-title":"Nat. Methods"},{"key":"10.1016\/j.stem.2025.08.006_bib87","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1158\/2159-8290.CD-16-0154","article-title":"Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR\/Cas9 Targeting","volume":"6","author":"Aguirre","year":"2016","journal-title":"Cancer Discov."},{"key":"10.1016\/j.stem.2025.08.006_bib88","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/nrg.2017.97","article-title":"Am I ready for CRISPR? A user\u2019s guide to genetic screens","volume":"19","author":"Doench","year":"2018","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.stem.2025.08.006_bib89","doi-asserted-by":"crossref","first-page":"691","DOI":"10.2217\/pgs.16.15","article-title":"Multilevel models improve precision and speed of IC50 estimates","volume":"17","author":"Vis","year":"2016","journal-title":"Pharmacogenomics"},{"key":"10.1016\/j.stem.2025.08.006_bib90","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/7651_2016_10","article-title":"Drug Sensitivity Assays of Human Cancer Organoid Cultures","volume":"1576","author":"Francies","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.stem.2025.08.006_bib91","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1038\/nature11005","article-title":"Systematic identification of genomic markers of drug sensitivity in cancer cells","volume":"483","author":"Garnett","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.stem.2025.08.006_bib92","doi-asserted-by":"crossref","DOI":"10.1186\/s12964-023-01096-2","article-title":"Serotonin acts through YAP to promote cell proliferation: mechanism and implication in colorectal cancer progression","volume":"21","author":"Yu","year":"2023","journal-title":"Cell Commun. Signal."},{"key":"10.1016\/j.stem.2025.08.006_bib93","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1111\/j.1440-1746.2008.05687.x","article-title":"Proliferative effects of gamma-aminobutyric acid on the gastric cancer cell line are associated with extracellular signal-regulated kinase 1\/2 activation","volume":"24","author":"Maemura","year":"2009","journal-title":"J. Gastroenterol. Hepatol."},{"key":"10.1016\/j.stem.2025.08.006_bib94","doi-asserted-by":"crossref","first-page":"2502","DOI":"10.1158\/1078-0432.CCR-07-1778","article-title":"Dopamine increases the efficacy of anticancer drugs in breast and colon cancer preclinical models","volume":"14","author":"Sarkar","year":"2008","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.stem.2025.08.006_bib95","doi-asserted-by":"crossref","first-page":"3946","DOI":"10.1038\/s41388-019-0709-6","article-title":"VPAC1 couples with TRPV4 channel to promote calcium-dependent gastric cancer progression via a novel autocrine mechanism","volume":"38","author":"Tang","year":"2019","journal-title":"Oncogene"},{"key":"10.1016\/j.stem.2025.08.006_bib96","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1038\/s41416-022-01916-1","article-title":"Neuropeptide Y, a paracrine factor secreted by cancer cells, is an independent regulator of angiogenesis in colon cancer","volume":"127","author":"Chakroborty","year":"2022","journal-title":"Br. J. Cancer"},{"key":"10.1016\/j.stem.2025.08.006_bib97","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/s41586-024-07767-5","article-title":"Neuronal substance P drives metastasis through an extracellular RNA-TLR7 axis","volume":"633","author":"Padmanaban","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.stem.2025.08.006_bib98","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.cyto.2009.03.010","article-title":"Tumor necrosis factor (TNF)-alpha-induced IL-8 expression in gastric epithelial cells: role of reactive oxygen species and AP endonuclease-1\/redox factor (Ref)-1","volume":"46","author":"O'Hara","year":"2009","journal-title":"Cytokine"},{"key":"10.1016\/j.stem.2025.08.006_bib99","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1089\/crispr.2021.0113","article-title":"Inference of CRISPR Edits from Sanger Trace Data","volume":"5","author":"Conant","year":"2022","journal-title":"CRISPR J."},{"key":"10.1016\/j.stem.2025.08.006_bib100","doi-asserted-by":"crossref","first-page":"505","DOI":"10.4161\/cc.8.3.7608","article-title":"Does the cell number 10(9) still really fit one gram of tumor tissue?","volume":"8","author":"Del Monte","year":"2009","journal-title":"Cell Cycle"},{"key":"10.1016\/j.stem.2025.08.006_bib101","first-page":"1","article-title":"Cholesterol Biosynthesis Inhibitor RO 48-8071 Suppresses Growth of Epithelial Ovarian Cancer Cells in Vitro and In Vivo","volume":"7","author":"Liang","year":"2023","journal-title":"J. Cancer Sci. Clin. Ther."}],"container-title":["Cell Stem Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S193459092500298X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S193459092500298X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T00:32:21Z","timestamp":1762561941000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S193459092500298X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":108,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["S193459092500298X"],"URL":"https:\/\/doi.org\/10.1016\/j.stem.2025.08.006","relation":{},"ISSN":["1934-5909"],"issn-type":[{"value":"1934-5909","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A combined enteric neuron-gastric tumor organoid reveals metabolic vulnerabilities in gastric cancer","name":"articletitle","label":"Article Title"},{"value":"Cell Stem Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.stem.2025.08.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}