{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T04:16:11Z","timestamp":1792383371651,"version":"4.4.0"},"reference-count":71,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T00:00:00Z","timestamp":1767398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["288034826-IRTG 2251"],"award-info":[{"award-number":["288034826-IRTG 2251"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Stem Cell"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.stem.2025.12.023","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T15:40:19Z","timestamp":1769010019000},"page":"325-339.e8","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["High-content screening of organoids reveals the mechanisms of human pancreas acinar specification"],"prefix":"10.1016","volume":"33","author":[{"given":"Rashmiparvathi","family":"Keshara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Kuodyte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antje","family":"Janosch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cordula","family":"Andree","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Bickle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"St\u00f6ter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rico","family":"Barsacchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yung Hae","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1202-5235","authenticated-orcid":false,"given":"Anne","family":"Grapin-Botton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.stem.2025.12.023_bib1","doi-asserted-by":"crossref","DOI":"10.1242\/dev.201004","article-title":"Pancreas organoid models of development and regeneration","volume":"149","author":"Grapin-Botton","year":"2022","journal-title":"Development"},{"key":"10.1016\/j.stem.2025.12.023_bib2","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/s43586-022-00174-y","article-title":"Organoids","volume":"2","author":"Zhao","year":"2022","journal-title":"Nat. Rev. Methods Primers"},{"key":"10.1016\/j.stem.2025.12.023_bib3","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1007\/s00109-020-02010-w","article-title":"Self-organization of organoids from endoderm-derived cells","volume":"99","author":"Lewis","year":"2021","journal-title":"J. Mol. Med. (Berl.)"},{"key":"10.1016\/j.stem.2025.12.023_bib4","doi-asserted-by":"crossref","first-page":"2756","DOI":"10.1016\/j.cell.2022.06.051","article-title":"Human organoids: New strategies and methods for analyzing human development and disease","volume":"185","author":"Corsini","year":"2022","journal-title":"Cell"},{"key":"10.1016\/j.stem.2025.12.023_bib5","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.stem.2021.11.001","article-title":"Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis","volume":"29","author":"Ungricht","year":"2022","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2025.12.023_bib6","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.stem.2020.02.007","article-title":"Genome-Scale CRISPR Screening in Human Intestinal Organoids Identifies Drivers of TGF-\u03b2 Resistance","volume":"26","author":"Ringel","year":"2020","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2025.12.023_bib7","doi-asserted-by":"crossref","DOI":"10.3389\/fceng.2023.1120348","article-title":"Organoids in high throughput and high-content screenings","volume":"5","author":"Lampart","year":"2023","journal-title":"Front. Chem. Eng."},{"key":"10.1016\/j.stem.2025.12.023_bib8","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1038\/s41586-020-2776-9","article-title":"Phenotypic landscape of intestinal organoid regeneration","volume":"586","author":"Lukonin","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.stem.2025.12.023_bib9","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1038\/s12276-021-00641-8","article-title":"Organoids in image-based phenotypic chemical screens","volume":"53","author":"Lukonin","year":"2021","journal-title":"Exp. Mol. Med."},{"key":"10.1016\/j.stem.2025.12.023_bib10","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1016\/j.stem.2023.04.018","article-title":"Multimodal characterization of murine gastruloid development","volume":"30","author":"Suppinger","year":"2023","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2025.12.023_bib11","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1038\/s41551-022-00863-9","article-title":"Screening for modulators of the cellular composition of gut epithelia via organoid models of intestinal stem cell differentiation","volume":"6","author":"Mead","year":"2022","journal-title":"Nat. Biomed. Eng."},{"key":"10.1016\/j.stem.2025.12.023_bib12","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1146\/annurev-cellbio-120320-035146","article-title":"Organoid Imaging: Seeing Development and Function","volume":"38","author":"Keshara","year":"2022","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"10.1016\/j.stem.2025.12.023_bib13","doi-asserted-by":"crossref","first-page":"3126","DOI":"10.1242\/dev.120063","article-title":"Human pancreas development","volume":"142","author":"Jennings","year":"2015","journal-title":"Development"},{"key":"10.1016\/j.stem.2025.12.023_bib14","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-021-23295-6","article-title":"A 3D system to model human pancreas development and its reference single-cell transcriptome atlas identify signaling pathways required for progenitor expansion","volume":"12","author":"Gon\u00e7alves","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2025.12.023_bib15","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1136\/gutjnl-2016-312423","article-title":"Human pluripotent stem cell-derived acinar\/ductal organoids generate human pancreas upon orthotopic transplantation and allow disease modelling","volume":"66","author":"Hohwieler","year":"2017","journal-title":"Gut"},{"key":"10.1016\/j.stem.2025.12.023_bib16","first-page":"793","article-title":"Sur la sph\u00e8re vide. A la m\u00e9moire de Georges Vorono\u00ef","volume":"6","author":"Delaunay","year":"1934","journal-title":"\u0418\u0437\u0432\u0435\u0441\u0442\u0438\u044f \u0420\u043e\u0441\u0441\u0438\u0439\u0441\u043a\u043e\u0439 \u0430\u043a\u0430\u0434\u0435\u043c\u0438\u0438 \u043d\u0430\u0443\u043a. \u0421\u0435\u0440\u0438\u044f \u043c\u0430\u0442\u0435\u043c\u0430\u0442\u0438\u0447\u0435\u0441\u043a\u0430\u044f"},{"key":"10.1016\/j.stem.2025.12.023_bib17","doi-asserted-by":"crossref","first-page":"861","DOI":"10.21105\/joss.00861","article-title":"Umap: Uniform manifold approximation and projection for dimension reduction","volume":"3","author":"McInnes","year":"2018","journal-title":"J. Open Source Softw."},{"key":"10.1016\/j.stem.2025.12.023_bib18","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.pharmthera.2014.11.016","article-title":"Glycogen synthase kinase-3 (GSK3): regulation, actions, and diseases","volume":"148","author":"Beurel","year":"2015","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.stem.2025.12.023_bib19","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1038\/35096075","article-title":"The renaissance of GSK3","volume":"2","author":"Cohen","year":"2001","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.stem.2025.12.023_bib20","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1038\/nbt.3033","article-title":"Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells","volume":"32","author":"Rezania","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.stem.2025.12.023_bib21","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1016\/j.stemcr.2017.08.009","article-title":"Single-Cell Gene Expression Analysis of a Human ESC Model of Pancreatic Endocrine Development Reveals Different Paths to beta-Cell Differentiation","volume":"9","author":"Petersen","year":"2017","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.stem.2025.12.023_bib22","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1091\/mbc.E14-12-1648","article-title":"Wnt ligand-dependent activation of the negative feedback regulator Nkd1","volume":"26","author":"Larraguibel","year":"2015","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.stem.2025.12.023_bib23","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1242\/dev.122.3.983","article-title":"PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum","volume":"122","author":"Offield","year":"1996","journal-title":"Development"},{"key":"10.1016\/j.stem.2025.12.023_bib24","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0960-9822(02)01434-3","article-title":"Experimental conversion of liver to pancreas","volume":"13","author":"Horb","year":"2003","journal-title":"Curr. Biol."},{"key":"10.1016\/j.stem.2025.12.023_bib25","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0925-4773(02)00337-4","article-title":"Transdifferentiation of pancreas to liver","volume":"120","author":"Shen","year":"2003","journal-title":"Mech. Dev."},{"key":"10.1016\/j.stem.2025.12.023_bib26","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1101\/gad.1360106","article-title":"Targeted deletion of a cis-regulatory region reveals differential gene dosage requirements for Pdx1 in foregut organ differentiation and pancreas formation","volume":"20","author":"Fujitani","year":"2006","journal-title":"Genes Dev."},{"key":"10.1016\/j.stem.2025.12.023_bib27","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1101\/gad.1412806","article-title":"Persistent expression of PDX-1 in the pancreas causes acinar-to-ductal metaplasia through Stat3 activation","volume":"20","author":"Miyatsuka","year":"2006","journal-title":"Genes Dev."},{"key":"10.1016\/j.stem.2025.12.023_bib28","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2022.1091757","article-title":"PDX1 is the cornerstone of pancreatic \u03b2-cell functions and identity","volume":"9","author":"Ebrahim","year":"2022","journal-title":"Front. Mol. Biosci."},{"key":"10.1016\/j.stem.2025.12.023_bib29","doi-asserted-by":"crossref","first-page":"1949","DOI":"10.7150\/thno.78323","article-title":"Single-cell profiling of GP2-enriched pancreatic progenitors to simultaneously create acinar, ductal, and endocrine organoids","volume":"13","author":"Merz","year":"2023","journal-title":"Theranostics"},{"key":"10.1016\/j.stem.2025.12.023_bib30","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1038\/nm.3973","article-title":"Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell- and patient-derived tumor organoids","volume":"21","author":"Huang","year":"2015","journal-title":"Nat. Med."},{"key":"10.1016\/j.stem.2025.12.023_bib31","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1016\/j.stem.2021.03.022","article-title":"Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids","volume":"28","author":"Huang","year":"2021","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2025.12.023_bib32","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-017-00258-4","article-title":"Stochastic priming and spatial cues orchestrate heterogeneous clonal contribution to mouse pancreas organogenesis","volume":"8","author":"Larsen","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2025.12.023_bib33","doi-asserted-by":"crossref","first-page":"5109","DOI":"10.1242\/dev.128.24.5109","article-title":"Fgf10 is essential for maintaining the proliferative capacity of epithelial progenitor cells during early pancreatic organogenesis","volume":"128","author":"Bhushan","year":"2001","journal-title":"Development"},{"key":"10.1016\/j.stem.2025.12.023_bib34","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-40481-1","article-title":"Adult human pancreatic acinar cells dedifferentiate into an embryonic progenitor-like state in 3D suspension culture","volume":"9","author":"Baldan","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.stem.2025.12.023_bib35","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/j.devcel.2007.04.001","article-title":"Functional redundancy of GSK-3alpha and GSK-3beta in Wnt\/beta-catenin signaling shown by using an allelic series of embryonic stem cell lines","volume":"12","author":"Doble","year":"2007","journal-title":"Dev. Cell"},{"key":"10.1016\/j.stem.2025.12.023_bib36","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1002\/dvdy.10157","article-title":"Expression patterns of Wnts, Frizzleds, sFRPs, and misexpression in transgenic mice suggesting a role for Wnts in pancreas and foregut pattern formation","volume":"225","author":"Heller","year":"2002","journal-title":"Dev. Dyn."},{"key":"10.1016\/j.stem.2025.12.023_bib37","doi-asserted-by":"crossref","first-page":"2207","DOI":"10.1242\/dev.001230","article-title":"Repression of Wnt\/\u03b2-catenin signaling in the anterior endoderm is essential for liver and pancreas development","volume":"134","author":"Mclin","year":"2007","journal-title":"Development"},{"key":"10.1016\/j.stem.2025.12.023_bib38","doi-asserted-by":"crossref","first-page":"51581","DOI":"10.1074\/jbc.M408830200","article-title":"Adenomatous polyposis coli control of retinoic acid biosynthesis is critical for zebrafish intestinal development and differentiation","volume":"279","author":"Nadauld","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.stem.2025.12.023_bib39","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1016\/j.stemcr.2024.05.010","article-title":"TGF-beta modulates cell fate in human ES cell-derived foregut endoderm by inhibiting Wnt and BMP signaling","volume":"19","author":"Funa","year":"2024","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.stem.2025.12.023_bib40","doi-asserted-by":"crossref","first-page":"4663","DOI":"10.1242\/dev.02063","article-title":"Beta-catenin is essential for pancreatic acinar but not islet development","volume":"132","author":"Murtaugh","year":"2005","journal-title":"Development"},{"key":"10.1016\/j.stem.2025.12.023_bib41","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1016\/j.cub.2005.08.037","article-title":"Pancreas-specific deletion of \u03b2-catenin reveals Wnt-dependent and Wnt-independent functions during development","volume":"15","author":"Dessimoz","year":"2005","journal-title":"Curr. Biol."},{"key":"10.1016\/j.stem.2025.12.023_bib42","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1242\/dev.02366","article-title":"Stabilization of \u03b2-catenin impacts pancreas growth","volume":"133","author":"Heiser","year":"2006","journal-title":"Development"},{"key":"10.1016\/j.stem.2025.12.023_bib43","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1471-213X-7-4","article-title":"Wnt\/\u03b2-catenin signaling is required for development of the exocrine pancreas","volume":"7","author":"Wells","year":"2007","journal-title":"BMC Dev. Biol."},{"key":"10.1016\/j.stem.2025.12.023_bib44","doi-asserted-by":"crossref","first-page":"2844","DOI":"10.2337\/diabetes.54.10.2844","article-title":"Attenuated Wnt signaling perturbs pancreatic growth but not pancreatic function","volume":"54","author":"Papadopoulou","year":"2005","journal-title":"Diabetes"},{"key":"10.1016\/j.stem.2025.12.023_bib45","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.ydbio.2014.03.019","article-title":"Distinct requirements for beta-catenin in pancreatic epithelial growth and patterning","volume":"391","author":"Baumgartner","year":"2014","journal-title":"Dev. Biol."},{"key":"10.1016\/j.stem.2025.12.023_bib46","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1016\/j.bbrc.2005.05.189","article-title":"A possible role for the canonical Wnt pathway in endocrine cell development in chicks","volume":"333","author":"Pedersen","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.stem.2025.12.023_bib47","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ceb.2019.07.002","article-title":"Wnt signaling: implications in endoderm development and pancreas organogenesis","volume":"61","author":"Scheibner","year":"2019","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.stem.2025.12.023_bib48","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1016\/j.celrep.2019.04.083","article-title":"Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development","volume":"27","author":"Sharon","year":"2019","journal-title":"Cell Rep."},{"key":"10.1016\/j.stem.2025.12.023_bib49","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.devcel.2007.06.001","article-title":"A multipotent progenitor domain guides pancreatic organogenesis","volume":"13","author":"Zhou","year":"2007","journal-title":"Dev. Cell"},{"key":"10.1016\/j.stem.2025.12.023_bib50","doi-asserted-by":"crossref","first-page":"3884","DOI":"10.1073\/pnas.062321799","article-title":"Role for FGFR2IIIb-mediated signals in controlling pancreatic endocrine progenitor cell proliferation","volume":"99","author":"Elghazi","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2025.12.023_bib51","doi-asserted-by":"crossref","first-page":"4452","DOI":"10.1242\/dev.096628","article-title":"Artificial three-dimensional niches deconstruct pancreas development in vitro","volume":"140","author":"Greggio","year":"2013","journal-title":"Development"},{"key":"10.1016\/j.stem.2025.12.023_bib52","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.mod.2009.11.005","article-title":"Conditional control of the differentiation competence of pancreatic endocrine and ductal cells by Fgf10","volume":"127","author":"Kobberup","year":"2010","journal-title":"Mech. Dev."},{"key":"10.1016\/j.stem.2025.12.023_bib53","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1073\/pnas.83.1.110","article-title":"Selective expression of rat pancreatic genes during embryonic development","volume":"83","author":"Han","year":"1986","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2025.12.023_bib54","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1053\/j.gastro.2011.04.050","article-title":"Lineage tracing evidence for transdifferentiation of acinar to duct cells and plasticity of human pancreas","volume":"141","author":"Houbracken","year":"2011","journal-title":"Gastroenterology"},{"key":"10.1016\/j.stem.2025.12.023_bib55","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1016\/j.jcmgh.2021.11.010","article-title":"On the Origin of Pancreatic Cancer: Molecular Tumor Subtypes in Perspective of Exocrine Cell Plasticity","volume":"13","author":"Backx","year":"2022","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"10.1016\/j.stem.2025.12.023_bib56","article-title":"Human pancreatic organoids derived from pluripotent stem cells recapitulate pancreatic organogenesis","author":"Brassard","year":"2025","journal-title":"bioRxiv"},{"key":"10.1016\/j.stem.2025.12.023_bib57","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.stem.2025.12.023_bib58","series-title":"Data Analysis, Machine Learning and Applications. Studies in Classification, Data Analysis, and Knowledge Organization","first-page":"319","article-title":"KNIME: The Konstanz Information Miner","author":"Berthold","year":"2007"},{"key":"10.1016\/j.stem.2025.12.023_bib59","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2006-7-10-r100","article-title":"CellProfiler: image analysis software for identifying and quantifying cell phenotypes","volume":"7","author":"Carpenter","year":"2006","journal-title":"Genome Biol."},{"key":"10.1016\/j.stem.2025.12.023_bib60","doi-asserted-by":"crossref","first-page":"2292","DOI":"10.1016\/j.devcel.2023.07.019","article-title":"Integrating single-cell imaging and RNA sequencing datasets links differentiation and morphogenetic dynamics of human pancreatic endocrine progenitors","volume":"58","author":"Beydag-Tas\u00f6z","year":"2023","journal-title":"Dev. Cell"},{"key":"10.1016\/j.stem.2025.12.023_bib61","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1016\/j.stemcr.2015.04.016","article-title":"A CRISPR\/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells","volume":"4","author":"Zhu","year":"2015","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.stem.2025.12.023_bib62","series-title":"Medical Image Computing and Computer Assisted Intervention - Miccai 2018","first-page":"265","article-title":"Cell Detection with Star-Convex Polygons","author":"Schmidt","year":"2018"},{"key":"10.1016\/j.stem.2025.12.023_bib63","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/978-1-4939-9145-7_4","article-title":"CellProfiler and KNIME: Open-Source Tools for High-Content Screening","volume":"1953","author":"St\u00f6ter","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.stem.2025.12.023_bib64","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1145\/235815.235821","article-title":"The Quickhull algorithm for convex hulls","volume":"22","author":"Barber","year":"1996","journal-title":"ACM Trans. Math. Softw."},{"key":"10.1016\/j.stem.2025.12.023_bib65","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s11222-007-9033-z","article-title":"A tutorial on spectral clustering","volume":"17","author":"von Luxburg","year":"2007","journal-title":"Stat. Comput."},{"key":"10.1016\/j.stem.2025.12.023_bib66","author":"Andrews"},{"key":"10.1016\/j.stem.2025.12.023_bib67","doi-asserted-by":"crossref","first-page":"D745","DOI":"10.1093\/nar\/gky1113","article-title":"Ensembl 2019","volume":"47","author":"Cunningham","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.stem.2025.12.023_bib68","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.stem.2025.12.023_bib69","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.stem.2025.12.023_bib70","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/nature01611","article-title":"Wnt proteins are lipid-modified and can act as stem cell growth factors","volume":"423","author":"Willert","year":"2003","journal-title":"Nature"},{"key":"10.1016\/j.stem.2025.12.023_bib71","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.stem.2009.11.013","article-title":"Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro","volume":"6","author":"Barker","year":"2010","journal-title":"Cell Stem Cell"}],"container-title":["Cell Stem Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1934590925004576?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1934590925004576?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T13:12:16Z","timestamp":1783170736000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1934590925004576"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":71,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["S1934590925004576"],"URL":"https:\/\/doi.org\/10.1016\/j.stem.2025.12.023","relation":{},"ISSN":["1934-5909"],"issn-type":[{"value":"1934-5909","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"High-content screening of organoids reveals the mechanisms of human pancreas acinar specification","name":"articletitle","label":"Article Title"},{"value":"Cell Stem Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.stem.2025.12.023","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}