{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:55:18Z","timestamp":1791982518503,"version":"4.4.0"},"reference-count":80,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001030","name":"National Heart Foundation of Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Stem Cell"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.stem.2026.07.010","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T16:11:54Z","timestamp":1786464714000},"page":"1528-1539.e10","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Human heart valve-like tissues from pluripotent stem cells with enhanced maturation recapitulate inflammatory valve disease"],"prefix":"10.1016","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5315-794X","authenticated-orcid":false,"given":"Holly K.","family":"Voges","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Durrant-Whyte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"See","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerii","family":"Iaprintsev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serene","family":"Yeow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin L.","family":"Parker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaitlyn","family":"Bibby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Keen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"King","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam T.","family":"Piers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita C.","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel G.","family":"Donner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akriti","family":"Varshney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Alaei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Yoannidis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Semple","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Charitakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerico","family":"Revote","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano","family":"Morandini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Purva","family":"Kumbhar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Sutton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Butera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edouard G.","family":"Stanley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice D.","family":"Reid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Hudson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amr H.","family":"Allam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina H.H.","family":"Le","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P.","family":"Burgess","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean J.","family":"Humphrey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Pepe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hazem","family":"Alkazemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel E.","family":"Heath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirana","family":"Ramialison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando J.","family":"Rossello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian P.","family":"Brizard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Elliott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Hidalgo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enzo R.","family":"Porrello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.stem.2026.07.010_bib1","doi-asserted-by":"crossref","first-page":"2178","DOI":"10.3390\/jcm12062178","article-title":"The Global Burden of Valvular Heart Disease: From Clinical Epidemiology to Management","volume":"12","author":"Santangelo","year":"2023","journal-title":"J. Clin. Med."},{"key":"10.1016\/j.stem.2026.07.010_bib2","doi-asserted-by":"crossref","DOI":"10.1161\/JAHA.124.036462","article-title":"Burden and Temporal Trends of Valvular Heart Disease-Related Heart Failure From 1990 to 2019 and Projection Up to 2030 in Group of 20 Countries: An Analysis for the Global Burden of Disease Study 2019","volume":"13","author":"Zhang","year":"2024","journal-title":"J. Am. Heart Assoc."},{"key":"10.1016\/j.stem.2026.07.010_bib3","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0195-668X(03)00201-X","article-title":"A prospective survey of patients with valvular heart disease in Europe: The Euro Heart Survey on Valvular Heart Disease","volume":"24","author":"Iung","year":"2003","journal-title":"Eur. Heart J."},{"key":"10.1016\/j.stem.2026.07.010_bib4","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.1161\/CIRCULATIONAHA.119.043391","article-title":"Global, Regional, and National Burden of Calcific Aortic Valve and Degenerative Mitral Valve Diseases, 1990\u20132017","volume":"141","author":"Yadgir","year":"2020","journal-title":"Circulation"},{"key":"10.1016\/j.stem.2026.07.010_bib5","doi-asserted-by":"crossref","DOI":"10.1161\/JAHA.122.025284","article-title":"Rheumatic Heart Disease Is a Neglected Disease Relative to Its Burden Worldwide: Findings From Global Burden of Disease 2019","volume":"11","author":"Ghamari","year":"2022","journal-title":"J. Am. Heart Assoc."},{"key":"10.1016\/j.stem.2026.07.010_bib6","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1093\/eurheartj\/ehab395","article-title":"ESC\/EACTS Guidelines for the management of valvular heart disease","volume":"43","author":"Vahanian","year":"2021","journal-title":"Eur. Heart J."},{"key":"10.1016\/j.stem.2026.07.010_bib7","series-title":"Biomaterials Science. An Introduction to Materials in Medicine","first-page":"3","article-title":"Introduction to Biomaterials Science: An Evolving Multidisciplinary Endeavor","author":"Ratner","year":"2020"},{"key":"10.1016\/j.stem.2026.07.010_bib8","doi-asserted-by":"crossref","first-page":"e183","DOI":"10.1016\/j.jtcvs.2021.04.002","article-title":"ACC\/AHA guideline for the management of patients with valvular heart disease: A report of the American College of Cardiology\/American Heart Association Joint Committee on Clinical Practice Guidelines","volume":"162","author":"Otto","year":"2021","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"10.1016\/j.stem.2026.07.010_bib9","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1007\/s11886-004-0006-y","article-title":"Newer concepts in the surgical treatment of valvular heart disease","volume":"6","author":"Sako","year":"2004","journal-title":"Curr. Cardiol. Rep."},{"key":"10.1016\/j.stem.2026.07.010_bib10","doi-asserted-by":"crossref","DOI":"10.1002\/btm2.10695","article-title":"Advances in xenogeneic donor decellularized organs: A review on studies with sheep and porcine-derived heart valves","volume":"9","author":"Inal","year":"2024","journal-title":"Bioeng. Transl. Med."},{"key":"10.1016\/j.stem.2026.07.010_bib11","first-page":"382","article-title":"Isolated aortic valve replacement with bio-prostheses in patients age 50 to 65 years: a decade of statewide data on cost and patient outcomes","volume":"63","author":"Quader","year":"2022","journal-title":"J. Cardiovasc. Surg. (Torino)"},{"key":"10.1016\/j.stem.2026.07.010_bib12","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1586\/erd.09.12","article-title":"Tissue engineering of heart valves: advances and current challenges","volume":"6","author":"Mol","year":"2009","journal-title":"Expert Rev. Med. Devices"},{"key":"10.1016\/j.stem.2026.07.010_bib13","article-title":"Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells","volume":"27","author":"Liu","year":"2024","journal-title":"iScience"},{"key":"10.1016\/j.stem.2026.07.010_bib14","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1038\/s42003-021-02571-7","article-title":"Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells","volume":"4","author":"Cheng","year":"2021","journal-title":"Commun. Biol."},{"key":"10.1016\/j.stem.2026.07.010_bib15","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-019-09459-5","article-title":"Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis","volume":"10","author":"Neri","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2026.07.010_bib16","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s13062-023-00390-w","article-title":"Single-cell transcriptomics reveals cellular heterogeneity and macrophage-to-mesenchymal transition in bicuspid calcific aortic valve disease","volume":"18","author":"Lyu","year":"2023","journal-title":"Biol. Direct"},{"key":"10.1016\/j.stem.2026.07.010_bib17","article-title":"Single-cell transcriptomics reveal cellular diversity of aortic valve and the immunomodulation by PPAR\u03b3 during hyperlipidemia","volume":"13","author":"Lee","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2026.07.010_bib18","doi-asserted-by":"crossref","first-page":"2910","DOI":"10.1161\/ATVBAHA.120.314789","article-title":"Cell-Type Transcriptome Atlas of Human Aortic Valves Reveal Cell Heterogeneity and Endothelial to Mesenchymal Transition Involved in Calcific Aortic Valve Disease","volume":"40","author":"Xu","year":"2020","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.stem.2026.07.010_bib19","article-title":"Assembly of Human Stem Cell-Derived Cortical Spheroids and Vascular Spheroids to Model 3-D Brain-like Tissues","volume":"9","author":"Song","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.stem.2026.07.010_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.jneumeth.2021.109254","article-title":"Increased maturation of iPSC-derived neurons in a hydrogel-based 3D culture","volume":"360","author":"de Leeuw","year":"2021","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.stem.2026.07.010_bib21","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/j.neuron.2017.07.035","article-title":"Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells","volume":"95","author":"Sloan","year":"2017","journal-title":"Neuron"},{"key":"10.1016\/j.stem.2026.07.010_bib22","article-title":"Mechanisms of heart valve development and disease","volume":"147","author":"O\u2019Donnell","year":"2020","journal-title":"Development"},{"key":"10.1016\/j.stem.2026.07.010_bib23","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.ydbio.2010.08.030","article-title":"VEGF signaling has distinct spatiotemporal roles during heart valve development","volume":"347","author":"Stankunas","year":"2010","journal-title":"Dev. Biol."},{"key":"10.1016\/j.stem.2026.07.010_bib24","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1161\/01.RES.0000141146.95728.da","article-title":"Heart valve development: endothelial cell signaling and differentiation","volume":"95","author":"Armstrong","year":"2004","journal-title":"Circ. Res."},{"key":"10.1016\/j.stem.2026.07.010_bib25","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1161\/01.RES.0000224114.65109.4e","article-title":"Extracellular Matrix Remodeling and Organization in Developing and Diseased Aortic Valves","volume":"98","author":"Hinton","year":"2006","journal-title":"Circ. Res."},{"key":"10.1016\/j.stem.2026.07.010_bib26","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1038\/s44161-024-00510-3","article-title":"APOE-NOTCH axis governs elastogenesis during human cardiac valve remodeling","volume":"3","author":"Liu","year":"2024","journal-title":"Nat CardioVasc Res."},{"key":"10.1016\/j.stem.2026.07.010_bib27","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2023.1102487","article-title":"Identifying molecular and functional similarities and differences between human primary cardiac valve interstitial cells and ventricular fibroblasts","volume":"11","author":"Floy","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.stem.2026.07.010_bib28","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1161\/CIRCULATIONAHA.122.063402","article-title":"Multiomics of Tissue Extracellular Vesicles Identifies Unique Modulators of Atherosclerosis and Calcific Aortic Valve Stenosis","volume":"148","author":"Blaser","year":"2023","journal-title":"Circulation"},{"key":"10.1016\/j.stem.2026.07.010_bib30","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-89164-w","article-title":"Collagen fiber regulation in human pediatric aortic valve development and disease","volume":"11","author":"Clift","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.stem.2026.07.010_bib31","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1161\/CIRCULATIONAHA.105.591768","article-title":"Human Semilunar Cardiac Valve Remodeling by Activated Cells From Fetus to Adult: Implications for Postnatal Adaptation, Pathology, and Tissue Engineering","volume":"113","author":"Aikawa","year":"2006","journal-title":"Circulation"},{"key":"10.1016\/j.stem.2026.07.010_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.autrev.2022.103209","article-title":"Cytokine imbalance in acute rheumatic fever and rheumatic heart disease: Mechanisms and therapeutic implications","volume":"21","author":"Middleton","year":"2022","journal-title":"Autoimmun. Rev."},{"key":"10.1016\/j.stem.2026.07.010_bib33","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1038\/s44161-026-00792-9","article-title":"Valve biology and rheumatic heart disease pathogenesis","volume":"5","author":"Yeow","year":"2026","journal-title":"Nat. Cardiovasc. Res."},{"key":"10.1016\/j.stem.2026.07.010_bib34","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms11703","article-title":"Tenascin-C drives persistence of organ fibrosis","volume":"7","author":"Bhattacharyya","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2026.07.010_bib35","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1002\/(SICI)1096-9896(199607)179:3<321::AID-PATH555>3.0.CO;2-8","article-title":"Tenascin and fibronectin expression in healing human myocardial scars","volume":"179","author":"Willems","year":"1996","journal-title":"J. Pathol."},{"key":"10.1016\/j.stem.2026.07.010_bib36","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1016\/S0735-1097(02)01792-8","article-title":"Active interstitial remodeling: an important process in the hibernating human myocardium","volume":"39","author":"Frangogiannis","year":"2002","journal-title":"J. Am. Coll. Cardiol."},{"key":"10.1016\/j.stem.2026.07.010_bib29","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1161\/ATVBAHA.122.318334","article-title":"Network-Guided Multiomic Mapping of Aortic Valve Calcification","volume":"43","author":"Blaser","year":"2023","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.stem.2026.07.010_bib37","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1161\/CIRCULATIONAHA.123.065143","article-title":"Directed Differentiation of Human Induced Pluripotent Stem Cells to Heart Valve Cells","volume":"149","author":"Cai","year":"2024","journal-title":"Circulation"},{"key":"10.1016\/j.stem.2026.07.010_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2023.112322","article-title":"Vascular cells improve functionality of human cardiac organoids","volume":"42","author":"Voges","year":"2023","journal-title":"Cell Rep."},{"key":"10.1016\/j.stem.2026.07.010_bib39","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-021-25329-5","article-title":"Self-assembling human heart organoids for the modeling of cardiac development and congenital heart disease","volume":"12","author":"Lewis-Israeli","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2026.07.010_bib40","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1016\/j.stem.2020.05.004","article-title":"Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease","volume":"26","author":"Giacomelli","year":"2020","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2026.07.010_bib41","doi-asserted-by":"crossref","DOI":"10.1242\/dev.173047","article-title":"Maturation of heart valve cell populations during postnatal remodeling","volume":"146","author":"Hulin","year":"2019","journal-title":"Development"},{"key":"10.1016\/j.stem.2026.07.010_bib42","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1093\/eurheartj\/ehac818","article-title":"Sortilin enhances fibrosis and calcification in aortic valve disease by inducing interstitial cell heterogeneity","volume":"44","author":"Iqbal","year":"2023","journal-title":"Eur. Heart J."},{"key":"10.1016\/j.stem.2026.07.010_bib43","doi-asserted-by":"crossref","first-page":"E8372","DOI":"10.1073\/pnas.1707316114","article-title":"Functional screening in human cardiac organoids reveals a metabolic mechanism for cardiomyocyte cell cycle arrest","volume":"114","author":"Mills","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stem.2026.07.010_bib44","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/j.stem.2019.03.009","article-title":"Drug Screening in Human PSC-Cardiac Organoids Identifies Pro-proliferative Compounds Acting via the Mevalonate Pathway","volume":"24","author":"Mills","year":"2019","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stem.2026.07.010_bib45","doi-asserted-by":"crossref","first-page":"D543","DOI":"10.1093\/nar\/gkae1011","article-title":"The PRIDE database at 20 years: 2025 update","volume":"53","author":"Perez-Riverol","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.stem.2026.07.010_bib46","doi-asserted-by":"crossref","DOI":"10.1016\/j.scr.2018.101380","article-title":"Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults","volume":"34","author":"Vlahos","year":"2019","journal-title":"Stem Cell Res."},{"key":"10.1016\/j.stem.2026.07.010_bib47","first-page":"1118","article-title":"Development of a human cardiac organoid injury model reveals innate regenerative potential","volume":"144","author":"Voges","year":"2017","journal-title":"Development"},{"key":"10.1016\/j.stem.2026.07.010_bib48","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2023.102371","article-title":"Generation of vascularized human cardiac organoids for 3D in vitro modeling","volume":"4","author":"Voges","year":"2023","journal-title":"Star Protoc."},{"key":"10.1016\/j.stem.2026.07.010_bib49","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.ejcts.2010.07.015","article-title":"High-concentration glutaraldehyde fixation of bovine pericardium in organic solvent and post-fixation glycine treatment: in vitro material assessment and in vivo anticalcification effect","volume":"39","author":"Lee","year":"2011","journal-title":"Eur. J. Cardiothorac. Surg."},{"key":"10.1016\/j.stem.2026.07.010_bib50","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/0142-9612(96)82721-9","article-title":"Glutaraldehyde as a fixative in bioprostheses and drug delivery matrices","volume":"17","author":"Jayakrishnan","year":"1996","journal-title":"Biomaterials"},{"key":"10.1016\/j.stem.2026.07.010_bib51","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1038\/s41587-023-02099-7","article-title":"Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition","volume":"42","author":"Guzman","year":"2024","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.stem.2026.07.010_bib52","doi-asserted-by":"crossref","first-page":"2296","DOI":"10.1074\/mcp.RA117.000314","article-title":"Optimization of Experimental Parameters in Data-Independent Mass Spectrometry Significantly Increases Depth and Reproducibility of Results","volume":"16","author":"Bruderer","year":"2017","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.stem.2026.07.010_bib53","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1038\/nbt.1511","article-title":"MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification","volume":"26","author":"Cox","year":"2008","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.stem.2026.07.010_bib54","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1038\/s41587-021-00968-7","article-title":"MaxDIA enables library-based and library-free data-independent acquisition proteomics","volume":"39","author":"Sinitcyn","year":"2021","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.stem.2026.07.010_bib55","doi-asserted-by":"crossref","first-page":"2513","DOI":"10.1074\/mcp.M113.031591","article-title":"Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ","volume":"13","author":"Cox","year":"2014","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.stem.2026.07.010_bib56","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1021\/acs.jproteome.8b00523","article-title":"NormalyzerDE: Online Tool for Improved Normalization of Omics Expression Data and High-Sensitivity Differential Expression Analysis","volume":"18","author":"Willforss","year":"2019","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.stem.2026.07.010_bib57","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkv007","article-title":"limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"Ritchie","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.stem.2026.07.010_bib58","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s13059-024-03219-5","article-title":"FixNCut: single-cell genomics through reversible tissue fixation and dissociation","volume":"25","author":"Jim\u00e9nez-Gracia","year":"2024","journal-title":"Genome Biol."},{"key":"10.1016\/j.stem.2026.07.010_bib59","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1161\/ATVBAHA.122.318314","article-title":"Cellular Landscapes of Nondiseased Human Cardiac Valves From End-Stage Heart Failure-Explanted Heart","volume":"42","author":"Shu","year":"2022","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.stem.2026.07.010_bib60","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1038\/s44161-025-00669-3","article-title":"Maturation of human cardiac organoids enables complex disease modeling and drug discovery","volume":"4","author":"Pocock","year":"2025","journal-title":"Nat CardioVasc Res."},{"key":"10.1016\/j.stem.2026.07.010_bib61","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1016\/S0003-4975(02)04489-2","article-title":"Calcification resistance with aluminum-ethanol treated porcine aortic valve bioprostheses in juvenile sheep","volume":"75","author":"Ogle","year":"2003","journal-title":"Ann. Thorac. Surg."},{"key":"10.1016\/j.stem.2026.07.010_bib62","doi-asserted-by":"crossref","first-page":"8356","DOI":"10.1039\/D1BM01296G","article-title":"Swim bladder as an alternative biomaterial for bioprosthetic valves","volume":"9","author":"Li","year":"2021","journal-title":"Biomater. Sci."},{"key":"10.1016\/j.stem.2026.07.010_bib63","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2021.766991","article-title":"Yak Pericardium as an Alternative Biomaterial for Transcatheter Heart Valves","volume":"9","author":"Song","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.stem.2026.07.010_bib64","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/s10047-014-0808-7","article-title":"Micro-CT evaluation of high pressure-decellularized cardiovascular tissues transplanted in rat subcutaneous accelerated-calcification model","volume":"18","author":"Mahara","year":"2015","journal-title":"J. Artif. Organs"},{"key":"10.1016\/j.stem.2026.07.010_bib65","doi-asserted-by":"crossref","DOI":"10.1016\/j.msec.2021.112329","article-title":"Zwitterionic hydrogel-coated heart valves with improved endothelialization and anti-calcification properties","volume":"128","author":"Luo","year":"2021","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"10.1016\/j.stem.2026.07.010_bib66","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1016\/S0003-4975(96)00442-0","article-title":"Impact of glutaraldehyde on calcification of pericardial bioprosthetic heart valve material","volume":"62","author":"Grabenw\u00f6ger","year":"1996","journal-title":"Ann. Thorac. Surg."},{"key":"10.1016\/j.stem.2026.07.010_bib67","doi-asserted-by":"crossref","first-page":"11882","DOI":"10.1039\/C9RA00408D","article-title":"Biofunctionalization of decellularized porcine aortic valve with OPG-loaded PCL nanoparticles for anti-calcification","volume":"9","author":"Li","year":"2019","journal-title":"RSC Adv."},{"key":"10.1016\/j.stem.2026.07.010_bib68","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1002\/(SICI)1097-4636(19990605)45:3<209::AID-JBM8>3.0.CO;2-N","article-title":"Mineralization of glutaraldehyde-fixed porcine aortic valve cusps in the subcutaneous rat model: analysis of variations in implant site and cuspal quadrants","volume":"45","author":"Mako","year":"1999","journal-title":"J. Biomed. Mater. Res."},{"key":"10.1016\/j.stem.2026.07.010_bib69","article-title":"Fibrous heart valve leaflet substrate with native-mimicked morphology","volume":"24","author":"Jana","year":"2021","journal-title":"Appl. Mater. Today"},{"key":"10.1016\/j.stem.2026.07.010_bib70","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/0021-9150(83)90163-6","article-title":"The identification of the vitamin k-dependent bone protein osteocalcin as one of the \u03b3-carboxyglutamic acid containing proteins present in calcified atherosclerotic plaque and mineralized heart valves","volume":"46","author":"Levy","year":"1983","journal-title":"Atherosclerosis"},{"key":"10.1016\/j.stem.2026.07.010_bib71","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1016\/j.cell.2021.03.026","article-title":"BET inhibition blocks inflammation-induced cardiac dysfunction and SARS-CoV-2 infection","volume":"184","author":"Mills","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.stem.2026.07.010_bib72","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41586-020-2901-9","article-title":"Identification of SARS-CoV-2 inhibitors using lung and colonic organoids","volume":"589","author":"Han","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.stem.2026.07.010_bib73","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-021-26410-9","article-title":"A human multi-lineage hepatic organoid model for liver fibrosis","volume":"12","author":"Guan","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stem.2026.07.010_bib74","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abq0866","article-title":"Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery","volume":"8","author":"Hiratsuka","year":"2022","journal-title":"Sci. Adv."},{"key":"10.1016\/j.stem.2026.07.010_bib75","doi-asserted-by":"crossref","first-page":"4547","DOI":"10.1016\/j.cell.2021.07.003","article-title":"ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids","volume":"184","author":"Bowles","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.stem.2026.07.010_bib76","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2026.115827","article-title":"Application of spatial transcriptomics across organoids for a high-resolution, spatial whole-transcriptome benchmarking dataset","volume":"29","author":"Nucera","year":"2026","journal-title":"iScience"},{"key":"10.1016\/j.stem.2026.07.010_bib77","doi-asserted-by":"crossref","DOI":"10.46471\/gigabyte.111","article-title":"SAW: an efficient and accurate data analysis workflow for Stereo-seq spatial transcriptomics","volume":"2024","author":"Gong","year":"2024","journal-title":"GigaByte"},{"key":"10.1016\/j.stem.2026.07.010_bib78","doi-asserted-by":"crossref","DOI":"10.1093\/bioadv\/vbac016","article-title":"decoupleR: ensemble of computational methods to infer biological activities from omics data","volume":"2","author":"Badia-I-Mompel","year":"2022","journal-title":"Bioinform. Adv."},{"key":"10.1016\/j.stem.2026.07.010_bib79","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s13059-017-1382-0","article-title":"SCANPY: large-scale single-cell gene expression data analysis","volume":"19","author":"Wolf","year":"2018","journal-title":"Genome Biol."},{"key":"10.1016\/j.stem.2026.07.010_bib80","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."}],"container-title":["Cell Stem Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1934590926002705?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1934590926002705?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:34:41Z","timestamp":1791981281000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1934590926002705"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":80,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["S1934590926002705"],"URL":"https:\/\/doi.org\/10.1016\/j.stem.2026.07.010","relation":{},"ISSN":["1934-5909"],"issn-type":[{"value":"1934-5909","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Human heart valve-like tissues from pluripotent stem cells with enhanced maturation recapitulate inflammatory valve disease","name":"articletitle","label":"Article Title"},{"value":"Cell Stem Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.stem.2026.07.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.stem.2026.07.011","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"https:\/\/doi.org\/10.1016\/j.stem.2026.08.003","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}