{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T15:18:50Z","timestamp":1790435930275,"version":"4.1.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T00:00:00Z","timestamp":1645488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["17933225"],"award-info":[{"award-number":["17933225"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP21gm1110008"],"award-info":[{"award-number":["JP21gm1110008"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["18H02935"],"award-info":[{"award-number":["18H02935"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["18H04882"],"award-info":[{"award-number":["18H04882"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["18H05281"],"award-info":[{"award-number":["18H05281"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["19H04906"],"award-info":[{"award-number":["19H04906"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["19H05750"],"award-info":[{"award-number":["19H05750"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["19K22512"],"award-info":[{"award-number":["19K22512"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Stem Cell Reports"],"published-print":{"date-parts":[[2022,4]]},"DOI":"10.1016\/j.stemcr.2022.02.013","type":"journal-article","created":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T23:05:38Z","timestamp":1648163138000},"page":"924-935","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":18,"title":["Regeneration of spermatogenesis by mouse germ cell transplantation into allogeneic and xenogeneic testis primordia or organoids"],"prefix":"10.1016","volume":"17","author":[{"given":"Mito","family":"Kanatsu-Shinohara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narumi","family":"Ogonuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shogo","family":"Matoba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Morimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Shiromoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuo","family":"Ogura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Shinohara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.stemcr.2022.02.013_bib1","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/337373a0","article-title":"Genetically haploid spermatids are phenotypically diploid","volume":"337","author":"Braun","year":"1989","journal-title":"Nature"},{"key":"10.1016\/j.stemcr.2022.02.013_bib2","doi-asserted-by":"crossref","first-page":"11298","DOI":"10.1073\/pnas.91.24.11298","article-title":"Spermatogenesis following male germ-cell transplantation","volume":"91","author":"Brinster","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stemcr.2022.02.013_bib3","doi-asserted-by":"crossref","first-page":"11303","DOI":"10.1073\/pnas.91.24.11303","article-title":"Germline transmission of donor haplotype following spermatogonial transplantation","volume":"91","author":"Brinster","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stemcr.2022.02.013_bib4","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1242\/dev.01555","article-title":"Spermatogenesis from epiblast and primordial germ cells following transplantation into postanal mouse testis","volume":"132","author":"Chuma","year":"2005","journal-title":"Development"},{"key":"10.1016\/j.stemcr.2022.02.013_bib5","first-page":"297","article-title":"Morphogenesis by dissociated immature rat testicular cells in primary culture","volume":"44","author":"Davis","year":"1978","journal-title":"J.\u00a0Embryol. Exp. Morph."},{"key":"10.1016\/j.stemcr.2022.02.013_bib6","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1242\/dev.146571","article-title":"The nature and dynamics of spermatogonial stem cells","volume":"144","author":"de Rooij","year":"2017","journal-title":"Development"},{"key":"10.1016\/j.stemcr.2022.02.013_bib7","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1095\/biolreprod61.5.1331","article-title":"Transplantation of germ cells from rabbits and dogs into mouse testes","volume":"61","author":"Dobrinski","year":"1999","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.stemcr.2022.02.013_bib8","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1093\/biolre\/ioy027","article-title":"50 years of spermatogenesis: Sertoli cells and their interactions with germ cells","volume":"99","author":"Griswold","year":"2018","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.stemcr.2022.02.013_bib9","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1083\/jcb.101.4.1511","article-title":"Extracellular matrix regulates Sertoli cell differentiation, testicular cord formation, and germ cell development in\u00a0vitro","volume":"101","author":"Hadley","year":"1985","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.stemcr.2022.02.013_bib10","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1095\/biolreprod.106.054999","article-title":"Building a testis: formation of functional testis tissue after transplantation of isolated porcine (Sus scrofa) testis cells","volume":"76","author":"Honaramooz","year":"2007","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.stemcr.2022.02.013_bib11","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.ceb.2021.06.007","article-title":"Organogenesis in\u00a0vitro","volume":"73","author":"Iwasaka","year":"2021","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.stemcr.2022.02.013_bib12","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1095\/biolreprod.103.017012","article-title":"Long-term proliferation and germline transmission of mouse male germline stem cells","volume":"69","author":"Kanatsu-Shinohara","year":"2003","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.stemcr.2022.02.013_bib13","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1146\/annurev-cellbio-101512-122353","article-title":"Spermatogonial stem cell self-renewal and development","volume":"29","author":"Kanatsu-Shinohara","year":"2013","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"10.1016\/j.stemcr.2022.02.013_bib14","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1262\/jrd.2018-093","article-title":"Reversible inhibition of the blood-testis barrier protein improves stem cell homing in mouse tetstes","volume":"64","author":"Kanatsu-Shinohara","year":"2018","journal-title":"J.\u00a0Reprod. Dev."},{"key":"10.1016\/j.stemcr.2022.02.013_bib15","doi-asserted-by":"crossref","first-page":"7834","DOI":"10.1073\/pnas.1914963117","article-title":"Autologous transplantation of spermatogonial stem cells restores fertility in congenitally infertile mice","volume":"117","author":"Kanatsu-Shinohara","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stemcr.2022.02.013_bib16","doi-asserted-by":"crossref","first-page":"2397","DOI":"10.1242\/dev.121.8.2397","article-title":"Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring","volume":"121","author":"Kimura","year":"1995","journal-title":"Development"},{"key":"10.1016\/j.stemcr.2022.02.013_bib17","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1095\/biolreprod.106.056895","article-title":"Production of functional spermatids from mouse germline stem cells in ectopically reconstituted seminiferous tubules","volume":"76","author":"Kita","year":"2007","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.stemcr.2022.02.013_bib18","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1095\/biolreprod.116.140277","article-title":"Spermatogenesis in explanted fetal mouse testis tissues","volume":"95","author":"Kojima","year":"2016","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.stemcr.2022.02.013_bib19","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/bs.ctdb.2015.12.009","article-title":"The curious world of gonadal development in mammals","volume":"116","author":"Koopman","year":"2016","journal-title":"Curr. Top. Dev. Biol."},{"key":"10.1016\/j.stemcr.2022.02.013_bib20","series-title":"Cell and Molecular Biology of the Testis","first-page":"266","article-title":"Spermatogonial stem cells","author":"Meistrich","year":"1993"},{"key":"10.1016\/j.stemcr.2022.02.013_bib21","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1073\/pnas.43.1.184","article-title":"Development in\u00a0vitro of chimaric aggregates of dissociated embryonic and mouse cells","volume":"43","author":"Moscona","year":"1957","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.stemcr.2022.02.013_bib22","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1101\/gad.339903.120","article-title":"An interplay of NOX1-derived ROS and oxygen determines the spermatogonial stem cell self-renewal efficiency under hypoxia","volume":"35","author":"Morimoto","year":"2021","journal-title":"Genes Dev."},{"key":"10.1016\/j.stemcr.2022.02.013_bib23","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1095\/biolreprod60.6.1429","article-title":"Pattern and kinetics of mouse donor spermatogonial stem cell colonization in recipient testes","volume":"60","author":"Nagano","year":"1999","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.stemcr.2022.02.013_bib24","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.ydbio.2005.09.008","article-title":"Gene expression during sex determination reveals a robust female genetic program at the onset of ovarian development","volume":"287","author":"Nef","year":"2005","journal-title":"Dev. Biol."},{"key":"10.1016\/j.stemcr.2022.02.013_bib25","first-page":"111","article-title":"Transplantation of testis germinal cells into mouse seminiferous tubules","volume":"41","author":"Ogawa","year":"1997","journal-title":"Int. J. Dev. Biol."},{"key":"10.1016\/j.stemcr.2022.02.013_bib26","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1095\/biolreprod60.2.515","article-title":"Xenogeneic spermatogenesis following transplantation of hamster germ cells to mouse testes","volume":"60","author":"Ogawa","year":"1999","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.stemcr.2022.02.013_bib27","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1054\/tice.1999.0060","article-title":"Recipient preparation is critical for spermatogonial transplantation in the rat","volume":"31","author":"Ogawa","year":"1999","journal-title":"Tissue Cell"},{"key":"10.1016\/j.stemcr.2022.02.013_bib28","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1095\/biolreprod.102.004549","article-title":"Expansion of murine spermatogonial stem cells through serial transplantation","volume":"68","author":"Ogawa","year":"2003","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.stemcr.2022.02.013_bib29","doi-asserted-by":"crossref","first-page":"13098","DOI":"10.1073\/pnas.0605755103","article-title":"Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring","volume":"103","author":"Ogonuki","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.stemcr.2022.02.013_bib30","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0074-7696(05)46005-2","article-title":"Microinsemination and nuclear transfer using male germ cells","volume":"246","author":"Ogura","year":"2005","journal-title":"Int. Rev. Cytol."},{"key":"10.1016\/j.stemcr.2022.02.013_bib31","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1159\/000130863","article-title":"Testicular cells lysostripped of H-Y antigen organize ovarian follicle-like aggreagtes","volume":"20","author":"Ohno","year":"1978","journal-title":"Cytogenet. Cell Genet."},{"key":"10.1016\/j.stemcr.2022.02.013_bib32","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.tem.2004.11.004","article-title":"Signaling at the crossroads of gonad development","volume":"16","author":"Ross","year":"2005","journal-title":"Trends Endocrinol. Metab."},{"key":"10.1016\/j.stemcr.2022.02.013_bib33","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1038\/nrg2295","article-title":"Epigenetic events in mammalian germ-cell development: reprogramming and beyond","volume":"9","author":"Sasaki","year":"2008","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.stemcr.2022.02.013_bib34","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature09850","article-title":"In\u00a0vitro production of functional sperm in cultured neonatal mouse testes","volume":"471","author":"Sato","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.stemcr.2022.02.013_bib35","doi-asserted-by":"crossref","first-page":"3039","DOI":"10.1093\/humrep\/17.12.3039","article-title":"Birth of offspring following transplantation of cryopreserved immature testicular pieces and in-vitro microinsemination","volume":"17","author":"Shinohara","year":"2002","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.stemcr.2022.02.013_bib36","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1095\/biolreprod.102.009977","article-title":"Restoration of spermatogenesis in infertile mice by Sertoli cell transplantation","volume":"68","author":"Shinohara","year":"2003","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.stemcr.2022.02.013_bib37","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/0027-5107(93)90159-D","article-title":"A quantitative study of spermatogonial multiplication and stem cell renewal in the F1 hybrid mouse","volume":"290","author":"Tegelenbosch","year":"1993","journal-title":"Mutat. Res."},{"key":"10.1016\/j.stemcr.2022.02.013_bib38","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1006\/excr.1999.4630","article-title":"An in\u00a0vitro tubule assay identifies HGF as a morphogen for the formation of seminiferous tubules in the postnatal mouse testis","volume":"252","author":"Van der Wee","year":"1995","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.stemcr.2022.02.013_bib39","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1095\/biolreprod.113.108613","article-title":"In\u00a0vitro reconstruction of mouse seminiferous tubules supporting germ cell differentiation","volume":"89","author":"Yokonishi","year":"2013","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.stemcr.2022.02.013_bib40","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1111\/j.1432-0436.1981.tb01170.x","article-title":"The capacity of testicular cells of the postnatal rata to reorganize into histotypic structures","volume":"20","author":"Zenzes","year":"1981","journal-title":"Differentiation"}],"container-title":["Stem Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213671122001023?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213671122001023?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T09:49:58Z","timestamp":1761990598000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2213671122001023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["S2213671122001023"],"URL":"https:\/\/doi.org\/10.1016\/j.stemcr.2022.02.013","relation":{},"ISSN":["2213-6711"],"issn-type":[{"value":"2213-6711","type":"print"}],"subject":[],"published":{"date-parts":[[2022,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Regeneration of spermatogenesis by mouse germ cell transplantation into allogeneic and xenogeneic testis primordia or organoids","name":"articletitle","label":"Article Title"},{"value":"Stem Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.stemcr.2022.02.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Author(s).","name":"copyright","label":"Copyright"}]}}