{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T15:53:04Z","timestamp":1790610784523,"version":"4.1.0"},"reference-count":31,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013294","name":"Helmholtz Zentrum Munchen Deutsches Forschungszentrum fur Umwelt und Gesundheit","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013294","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018693","name":"Horizon 2020","doi-asserted-by":"publisher","award":["874839"],"award-info":[{"award-number":["874839"]}],"id":[{"id":"10.13039\/100018693","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013295","name":"Helmholtz Zentrum M\u00fcnchen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013295","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Forschung, Technologie und Raumfahrt","doi-asserted-by":"publisher","award":["031L0251"],"award-info":[{"award-number":["031L0251"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Stem Cell Reports"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1016\/j.stemcr.2026.102828","type":"journal-article","created":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T15:54:25Z","timestamp":1772726065000},"page":"102828","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["CD133\/CD49a discriminate between human pluripotent stem cell-derived pancreatic beta and alpha cells"],"prefix":"10.1016","volume":"21","author":[{"given":"Chenglei","family":"Tian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilin","family":"Di","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aisha","family":"Muhammad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6747-787X","authenticated-orcid":false,"given":"Henrik","family":"Semb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.stemcr.2026.102828_bib1","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1038\/s41556-023-01150-8","article-title":"Single-nucleus multi-omics of human stem cell-derived islets identifies deficiencies in lineage specification","volume":"25","author":"Augsornworawat","year":"2023","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.stemcr.2026.102828_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2020.108067","article-title":"Single-Cell Transcriptome Profiling Reveals beta Cell Maturation in Stem Cell-Derived Islets after Transplantation","volume":"32","author":"Augsornworawat","year":"2020","journal-title":"Cell Rep."},{"key":"10.1016\/j.stemcr.2026.102828_bib3","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1038\/s41587-022-01219-z","article-title":"Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells","volume":"40","author":"Balboa","year":"2022","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.stemcr.2026.102828_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.molmet.2020.101060","article-title":"Purification of pancreatic endocrine subsets reveals increased iron metabolism in beta cells","volume":"42","author":"Berthault","year":"2020","journal-title":"Mol. Metabol."},{"key":"10.1016\/j.stemcr.2026.102828_bib5","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1016\/j.febslet.2010.01.050","article-title":"The intriguing links between prominin-1 (CD133), cholesterol-based membrane microdomains, remodeling of apical plasma membrane protrusions, extracellular membrane particles, and (neuro)epithelial cell differentiation","volume":"584","author":"Corbeil","year":"2010","journal-title":"FEBS Lett."},{"key":"10.1016\/j.stemcr.2026.102828_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2022.110425","article-title":"An HNF1alpha truncation associated with maturity-onset diabetes of the young impairs pancreatic progenitor differentiation by antagonizing HNF1beta function","volume":"38","author":"Cujba","year":"2022","journal-title":"Cell Rep."},{"key":"10.1016\/j.stemcr.2026.102828_bib7","doi-asserted-by":"crossref","first-page":"2554","DOI":"10.2337\/db20-0873","article-title":"ENTPD3 Marks Mature Stem Cell-Derived beta-Cells Formed by Self-Aggregation In Vitro","volume":"70","author":"Docherty","year":"2021","journal-title":"Diabetes"},{"key":"10.1016\/j.stemcr.2026.102828_bib8","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1038\/s41591-021-01645-7","article-title":"Human pluripotent stem-cell-derived islets ameliorate diabetes in non-human primates","volume":"28","author":"Du","year":"2022","journal-title":"Nat. Med."},{"key":"10.1016\/j.stemcr.2026.102828_bib9","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-09946-z","article-title":"ITGA1 is a pre-malignant biomarker that promotes therapy resistance and metastatic potential in pancreatic cancer","volume":"7","author":"Gharibi","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.stemcr.2026.102828_bib10","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.stem.2022.12.001","article-title":"An insulin hypersecretion phenotype precedes pancreatic beta cell failure in MODY3 patient-specific cells","volume":"30","author":"Hermann","year":"2023","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.stemcr.2026.102828_bib11","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2320883121","article-title":"Identification and removal of unexpected proliferative off-target cells emerging after iPSC-derived pancreatic islet cell implantation","volume":"121","author":"Hiyoshi","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stemcr.2026.102828_bib12","doi-asserted-by":"crossref","first-page":"4740","DOI":"10.1038\/s41598-022-08753-5","article-title":"Characterization and reduction of non-endocrine cells accompanying islet-like endocrine cells differentiated from human iPSC","volume":"12","author":"Hiyoshi","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.stemcr.2026.102828_bib13","article-title":"A Compound Screen Based on Isogenic hESC-Derived beta Cell Reveals an Inhibitor Targeting ZnT8-Mediated Zinc Transportation to Protect Pancreatic beta Cell from Stress-Induced Cell Death","volume":"12","author":"Hu","year":"2025","journal-title":"Adv. Sci."},{"key":"10.1016\/j.stemcr.2026.102828_bib14","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.devcel.2007.02.010","article-title":"Temporal control of neurogenin3 activity in pancreas progenitors reveals competence windows for the generation of different endocrine cell types","volume":"12","author":"Johansson","year":"2007","journal-title":"Dev. Cell"},{"key":"10.1016\/j.stemcr.2026.102828_bib15","doi-asserted-by":"crossref","first-page":"9771","DOI":"10.1038\/s41467-024-54151-y","article-title":"A noncoding variant confers pancreatic differentiation defect and contributes to diabetes susceptibility by recruiting RXRA","volume":"15","author":"Li","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stemcr.2026.102828_bib16","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1038\/ncb3628","article-title":"EGFR signalling controls cellular fate and pancreatic organogenesis by regulating apicobasal polarity","volume":"19","author":"Lof-Ohlin","year":"2017","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.stemcr.2026.102828_bib17","doi-asserted-by":"crossref","first-page":"e171","DOI":"10.1002\/term.2329","article-title":"CD99 as surface anchor for human islet endocrine cell purification","volume":"12","author":"Martens","year":"2018","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"10.1016\/j.stemcr.2026.102828_bib18","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2021.635405","article-title":"Selection for CD26(-) and CD49A(+) Cells From Pluripotent Stem Cells-Derived Islet-Like Clusters Improves Therapeutic Activity in Diabetic Mice","volume":"12","author":"Molakandov","year":"2021","journal-title":"Front. Endocrinol."},{"key":"10.1016\/j.stemcr.2026.102828_bib19","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1038\/s41556-018-0271-4","article-title":"Recapitulating endocrine cell clustering in culture promotes maturation of human stem-cell-derived beta cells","volume":"21","author":"Nair","year":"2019","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.stemcr.2026.102828_bib20","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.devcel.2019.02.005","article-title":"p120ctn-Mediated Organ Patterning Precedes and Determines Pancreatic Progenitor Fate","volume":"49","author":"Nyeng","year":"2019","journal-title":"Dev. Cell"},{"key":"10.1016\/j.stemcr.2026.102828_bib21","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1016\/j.stemcr.2022.02.001","article-title":"Development of a scalable method to isolate subsets of stem cell-derived pancreatic islet cells","volume":"17","author":"Parent","year":"2022","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.stemcr.2026.102828_bib22","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1038\/s41467-020-16049-3","article-title":"A method for the generation of human stem cell-derived alpha cells","volume":"11","author":"Peterson","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.stemcr.2026.102828_bib23","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1038\/nbt.3033","article-title":"Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells","volume":"32","author":"Rezania","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.stemcr.2026.102828_bib24","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.15252\/embj.201591058","article-title":"Controlled induction of human pancreatic progenitors produces functional beta-like cells in vitro","volume":"34","author":"Russ","year":"2015","journal-title":"EMBO J."},{"key":"10.1016\/j.stemcr.2026.102828_bib25","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.cmet.2018.10.007","article-title":"Ectonucleoside Triphosphate Diphosphohydrolase-3 Antibody Targets Adult Human Pancreatic beta Cells for In Vitro and In Vivo Analysis","volume":"29","author":"Saunders","year":"2019","journal-title":"Cell Metab."},{"key":"10.1016\/j.stemcr.2026.102828_bib26","doi-asserted-by":"crossref","DOI":"10.1111\/jpi.70073","article-title":"Modeling Genetic Risk of beta-Cell Dysfunction in Human Induced Pluripotent Stem Cells From Patients Carrying the MTNR1B Risk Variant","volume":"77","author":"Singh","year":"2025","journal-title":"J. Pineal Res."},{"key":"10.1016\/j.stemcr.2026.102828_bib27","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1073\/pnas.0609490104","article-title":"Conserved markers of fetal pancreatic epithelium permit prospective isolation of islet progenitor cells by FACS","volume":"104","author":"Sugiyama","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.stemcr.2026.102828_bib28","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1016\/j.devcel.2025.02.008","article-title":"Pancreatic alpha and beta cell fate choice is directed by apical-basal polarity dynamics","volume":"60","author":"Tiemann","year":"2025","journal-title":"Dev. Cell"},{"key":"10.1016\/j.stemcr.2026.102828_bib29","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1093\/icb\/43.1.55","article-title":"Epithelium--the primary building block for metazoan complexity","volume":"43","author":"Tyler","year":"2003","journal-title":"Integr. Comp. Biol."},{"key":"10.1016\/j.stemcr.2026.102828_bib30","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.stemcr.2018.12.012","article-title":"Acquisition of Dynamic Function in Human Stem Cell-Derived beta Cells","volume":"12","author":"Velazco-Cruz","year":"2019","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.stemcr.2026.102828_bib31","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1038\/s41586-019-1168-5","article-title":"Charting cellular identity during human in vitro beta-cell differentiation","volume":"569","author":"Veres","year":"2019","journal-title":"Nature"}],"container-title":["Stem Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213671126000391?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213671126000391?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T09:44:51Z","timestamp":1777628691000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2213671126000391"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["S2213671126000391"],"URL":"https:\/\/doi.org\/10.1016\/j.stemcr.2026.102828","relation":{},"ISSN":["2213-6711"],"issn-type":[{"value":"2213-6711","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"CD133\/CD49a discriminate between human pluripotent stem cell-derived pancreatic beta and alpha cells","name":"articletitle","label":"Article Title"},{"value":"Stem Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.stemcr.2026.102828","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc. on behalf of International Society for Stem Cell Research.","name":"copyright","label":"Copyright"}],"article-number":"102828"}}