{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:18:40Z","timestamp":1767917920203,"version":"3.49.0"},"reference-count":37,"publisher":"Elsevier BV","issue":"13","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Steroids"],"published-print":{"date-parts":[[2008,12]]},"DOI":"10.1016\/j.steroids.2008.05.004","type":"journal-article","created":{"date-parts":[[2008,5,22]],"date-time":"2008-05-22T08:53:12Z","timestamp":1211446392000},"page":"1345-1352","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":65,"title":["Validation of a testosterone and dihydrotestosterone liquid chromatography tandem mass spectrometry assay: Interference and comparison with established methods"],"prefix":"10.1016","volume":"73","author":[{"given":"Christina","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve","family":"Shiraishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Leung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sima","family":"Baravarian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Hull","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Goh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul W.N.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald S.","family":"Swerdloff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.steroids.2008.05.004_bib1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S0039-128X(01)00147-7","article-title":"Measurement of steroid sex hormones in serum: a comparison of radioimmunoassay and mass spectrometry","volume":"67","author":"Dorgan","year":"2002","journal-title":"Steroids"},{"key":"10.1016\/j.steroids.2008.05.004_bib2","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1093\/clinchem\/42.5.749","article-title":"Serum total testosterone: immunoassay compared with negative chemical ionization gas chromatography\u2013mass spectrometry","volume":"42","author":"Fitzgerald","year":"1996","journal-title":"Clin Chem"},{"key":"10.1016\/j.steroids.2008.05.004_bib3","doi-asserted-by":"crossref","first-page":"5928","DOI":"10.1210\/jc.2005-0962","article-title":"Reproductive hormone reference intervals for healthy fertile young men: evaluation of automated platform assays","volume":"90","author":"Sikaris","year":"2005","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1016\/j.steroids.2008.05.004_bib4","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1373\/49.8.1381","article-title":"Testosterone measured by 10 immunoassays and by isotope-dilution gas chromatography-mass spectrometry in sera from 116 men, women, and children","volume":"49","author":"Taieb","year":"2003","journal-title":"Clin Chem"},{"key":"10.1016\/j.steroids.2008.05.004_bib5","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1210\/jc.2003-031287","article-title":"Measurement of total serum testosterone in adult men: comparison of current laboratory methods versus liquid chromatography\u2013tandem mass spectrometry","volume":"89","author":"Wang","year":"2004","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1016\/j.steroids.2008.05.004_bib6","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.1210\/jc.2005-2847","article-title":"Testosterone therapy in adult men with androgen deficiency syndromes: an endocrine society clinical practice guideline","volume":"91","author":"Bhasin","year":"2006","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1016\/j.steroids.2008.05.004_bib7","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1210\/jc.2006-1864","article-title":"Utility, limitations, and pitfalls in measuring testosterone: an endocrine society position statement","volume":"92","author":"Rosner","year":"2007","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1016\/j.steroids.2008.05.004_bib8","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1172\/JCI106391","article-title":"Dihydrotestosterone in prostatic hypertrophy. I. The formation and content of dihydrotestosterone in the hypertrophic prostate of man","volume":"49","author":"Siiteri","year":"1970","journal-title":"J Clin Invest"},{"key":"10.1016\/j.steroids.2008.05.004_bib9","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1056\/NEJMoa030660","article-title":"The influence of finasteride on the development of prostate cancer","volume":"349","author":"Thompson","year":"2003","journal-title":"N Engl J Med"},{"key":"10.1016\/j.steroids.2008.05.004_bib10","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/S0009-9120(75)93886-2","article-title":"Combined radioimmunoassay of four steroids in one ml of plasma. II. Androgens","volume":"8","author":"Abraham","year":"1975","journal-title":"Clin Biochem"},{"key":"10.1016\/j.steroids.2008.05.004_bib11","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/0010-7824(82)90041-5","article-title":"A rapid method for the determination of 5 alpha-dihydrotestosterone in Thai males receiving medroxyprogesterone acetate","volume":"25","author":"Werawatgoompa","year":"1982","journal-title":"Contraception"},{"key":"10.1016\/j.steroids.2008.05.004_bib12","unstructured":"Shiraishi S, Lee PWN, Leung A, Goh VHH, Swerdloff RS, Wang C. Simultaneous measurement of serum testosterone and dihydrotestosterone by liquid chromatography tandem mass spectrometry. Clin Chem; in press."},{"key":"10.1016\/j.steroids.2008.05.004_bib13","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1039\/b210111b","article-title":"Quantitative measurement of male steroid hormones using automated on-line solid phase extraction-liquid chromatography-tandem mass spectrometry and comparison with radioimmunoassay","volume":"128","author":"Chang","year":"2003","journal-title":"Analyst"},{"key":"10.1016\/j.steroids.2008.05.004_bib14","doi-asserted-by":"crossref","first-page":"3200","DOI":"10.1002\/rcm.3205","article-title":"Analysis of testosterone and dihydrotestosterone from biological fluids as the oxime derivatives using high-performance liquid chromatography\/tandem mass spectrometry","volume":"21","author":"Kalhorn","year":"2007","journal-title":"Rapid Commun Mass Spectrom"},{"key":"10.1016\/j.steroids.2008.05.004_bib15","doi-asserted-by":"crossref","first-page":"586","DOI":"10.2164\/jandrol.04164","article-title":"Changes in testosterone and dihydrotestosterone levels in male rat accessory sex organs, serum, and seminal fluid after castration: establishment of a new highly sensitive simultaneous androgen measurement method","volume":"26","author":"Kashiwagi","year":"2005","journal-title":"J Androl"},{"key":"10.1016\/j.steroids.2008.05.004_bib16","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.steroids.2004.05.020","article-title":"Simultaneous determination of steroid composition of human testicular fluid using liquid chromatography tandem mass spectrometry","volume":"69","author":"Zhao","year":"2004","journal-title":"Steroids"},{"key":"10.1016\/j.steroids.2008.05.004_bib17","first-page":"4500","article-title":"Long-term pharmacokinetics of transdermal testosterone gel in hypogonadal men","volume":"85","author":"Swerdloff","year":"2000","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1016\/j.steroids.2008.05.004_bib18","first-page":"2749","article-title":"Comparative pharmacokinetics of three doses of percutaneous dihydrotestosterone gel in healthy elderly men\u2014a clinical research center study","volume":"83","author":"Wang","year":"1998","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1016\/j.steroids.2008.05.004_bib19","first-page":"964","article-title":"Pharmacokinetics of transdermal testosterone gel in hypogonadal men: application of gel at one site versus four sites\u2014a General Clinical Research Center Study","volume":"85","author":"Wang","year":"2000","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1016\/j.steroids.2008.05.004_bib20","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0009-8981(75)90199-0","article-title":"Applications of statistics in clinical chemistry. A critical evaluation of regression lines","volume":"64","author":"Wakkers","year":"1975","journal-title":"Clin Chim Acta"},{"key":"10.1016\/j.steroids.2008.05.004_bib21","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1093\/clinchem\/25.3.432","article-title":"Incorrect least-squares regression coefficients in method-comparison analysis","volume":"25","author":"Cornbleet","year":"1979","journal-title":"Clin Chem"},{"key":"10.1016\/j.steroids.2008.05.004_bib22","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1007\/BF02918550","article-title":"Use of Deming regression in method-comparison studies","volume":"1","author":"Konings","year":"1982","journal-title":"Surv Immunol Res"},{"key":"10.1016\/j.steroids.2008.05.004_bib23","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0140-6736(86)90837-8","article-title":"Statistical methods for assessing agreement between two methods of clinical measurement","volume":"1","author":"Bland","year":"1986","journal-title":"Lancet"},{"key":"10.1016\/j.steroids.2008.05.004_bib24","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1177\/096228029900800204","article-title":"Measuring agreement in method comparison studies","volume":"8","author":"Bland","year":"1999","journal-title":"Stat Methods Med Res"},{"key":"10.1016\/j.steroids.2008.05.004_bib25","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1373\/clinchem.2005.052167","article-title":"Performance characteristics of a novel tandem mass spectrometry assay for serum testosterone","volume":"52","author":"Kushnir","year":"2006","journal-title":"Clin Chem"},{"key":"10.1016\/j.steroids.2008.05.004_bib26","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1007\/s00216-007-1355-3","article-title":"Development and evaluation of a candidate reference measurement procedure for the determination of testosterone in human serum using isotope dilution liquid chromatography\/tandem mass spectrometry","volume":"388","author":"Tai","year":"2007","journal-title":"Anal Bioanal Chem"},{"key":"10.1016\/j.steroids.2008.05.004_bib27","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1210\/jcem-51-6-1335","article-title":"Treatment of male hypogonadism with testosterone enanthate","volume":"51","author":"Snyder","year":"1980","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1016\/j.steroids.2008.05.004_bib28","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/S0015-0282(16)46108-X","article-title":"Comparison of the kinetics of injectable testosterone in eugonadal and hypogonadal men","volume":"37","author":"Sokol","year":"1982","journal-title":"Fertil Steril"},{"key":"10.1016\/j.steroids.2008.05.004_bib29","doi-asserted-by":"crossref","first-page":"2610","DOI":"10.1210\/jc.2004-1221","article-title":"Oral testosterone in oil plus dutasteride in men: a pharmacokinetic study","volume":"90","author":"Amory","year":"2005","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1016\/j.steroids.2008.05.004_bib30","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1002\/j.1939-4640.1994.tb00433.x","article-title":"A ten-year safety study of the oral androgen testosterone undecanoate","volume":"15","author":"Gooren","year":"1994","journal-title":"J Androl"},{"key":"10.1016\/j.steroids.2008.05.004_bib31","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1365-2265.1981.tb00364.x","article-title":"Androgen replacement with oral testosterone undecanoate in hypogonadal men: a double blind controlled study","volume":"14","author":"Skakkebaek","year":"1981","journal-title":"Clin Endocrinol (Oxf)"},{"key":"10.1016\/j.steroids.2008.05.004_bib32","first-page":"456","article-title":"Saliva and serum testosterone following oral testosterone undecanoate administration in normal and hypogonadal men","volume":"102","author":"Schurmeyer","year":"1983","journal-title":"Acta Endocrinol (Copenh)"},{"key":"10.1016\/j.steroids.2008.05.004_bib33","doi-asserted-by":"crossref","first-page":"5429","DOI":"10.1210\/jc.2004-0897","article-title":"Intramuscular testosterone undecanoate: pharmacokinetic aspects of a novel testosterone formulation during long-term treatment of men with hypogonadism","volume":"89","author":"Schubert","year":"2004","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1016\/j.steroids.2008.05.004_bib34","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1517\/14656566.6.9.1493","article-title":"Advances in male hormone substitution therapy","volume":"6","author":"Qoubaitary","year":"2005","journal-title":"Expert Opin Pharmacother"},{"key":"10.1016\/j.steroids.2008.05.004_bib35","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1158\/1055-9965.EPI-06-0792","article-title":"Reproducibility of serum sex steroid assays in men by RIA and mass spectrometry","volume":"16","author":"Hsing","year":"2007","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"10.1016\/j.steroids.2008.05.004_bib36","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1023\/A:1007669411738","article-title":"Bioanalytical method validation\u2014a revisit with a decade of progress","volume":"17","author":"Shah","year":"2000","journal-title":"Pharm Res"},{"key":"10.1016\/j.steroids.2008.05.004_bib37","doi-asserted-by":"crossref","first-page":"1962","DOI":"10.1007\/s11095-007-9291-7","article-title":"Quantitative bioanalytical methods validation and implementation: best practices for chromatographic and ligand binding assays","volume":"24","author":"Viswanathan","year":"2007","journal-title":"Pharm Res"}],"updated-by":[{"DOI":"10.1016\/j.steroids.2018.05.006","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000}}],"container-title":["Steroids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039128X08001426?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039128X08001426?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T10:00:38Z","timestamp":1758535238000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039128X08001426"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12]]},"references-count":37,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["S0039128X08001426"],"URL":"https:\/\/doi.org\/10.1016\/j.steroids.2008.05.004","relation":{},"ISSN":["0039-128X"],"issn-type":[{"value":"0039-128X","type":"print"}],"subject":[],"published":{"date-parts":[[2008,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Validation of a testosterone and dihydrotestosterone liquid chromatography tandem mass spectrometry assay: Interference and comparison with established methods","name":"articletitle","label":"Article Title"},{"value":"Steroids","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.steroids.2008.05.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2008 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}