{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:26:46Z","timestamp":1791350806474,"version":"4.3.1"},"reference-count":47,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,4,8]],"date-time":"2015-04-08T00:00:00Z","timestamp":1428451200000},"content-version":"vor","delay-in-days":372,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Structure"],"published-print":{"date-parts":[[2014,4]]},"DOI":"10.1016\/j.str.2014.02.013","type":"journal-article","created":{"date-parts":[[2014,3,27]],"date-time":"2014-03-27T12:54:52Z","timestamp":1395924892000},"page":"590-601","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":91,"title":["Structural Basis of Substrate Specificity of Human Oligosaccharyl Transferase Subunit N33\/Tusc3 and Its Role in Regulating Protein N-Glycosylation"],"prefix":"10.1016","volume":"22","author":[{"given":"Elisabeth","family":"Mohorko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin\u00a0L.","family":"Owen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goran","family":"Maloj\u010di\u0107","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurice\u00a0S.","family":"Brozzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Aebi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudi","family":"Glockshuber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.str.2014.02.013_bib1","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1593\/neo.04586","article-title":"Array-based comparative genomic hybridization identifies localized DNA amplifications and homozygous deletions in pancreatic cancer","volume":"7","author":"Bashyam","year":"2005","journal-title":"Neoplasia"},{"key":"10.1016\/j.str.2014.02.013_bib2","series-title":"Biochemistry","author":"Berg","year":"2002"},{"key":"10.1016\/j.str.2014.02.013_bib3","doi-asserted-by":"crossref","first-page":"5768","DOI":"10.1021\/cr100006x","article-title":"Reactivity of thioredoxin as a protein thiol-disulfide oxidoreductase","volume":"111","author":"Cheng","year":"2011","journal-title":"Chem. Rev."},{"key":"10.1016\/j.str.2014.02.013_bib4","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1186\/1471-2407-8-288","article-title":"High-resolution array CGH clarifies events occurring on 8p in carcinogenesis","volume":"8","author":"Cooke","year":"2008","journal-title":"BMC Cancer"},{"key":"10.1016\/j.str.2014.02.013_bib5","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0304-4165(99)00167-1","article-title":"Glycoprotein glycosylation and cancer progression","volume":"1473","author":"Dennis","year":"1999","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.str.2014.02.013_bib6","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1002\/(SICI)1521-1878(199905)21:5<412::AID-BIES8>3.0.CO;2-5","article-title":"Protein glycosylation in development and disease","volume":"21","author":"Dennis","year":"1999","journal-title":"Bioessays"},{"key":"10.1016\/j.str.2014.02.013_bib7","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/S0968-0004(96)10034-7","article-title":"Why mammalian cell surface proteins are glycoproteins","volume":"21","author":"Gahmberg","year":"1996","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.str.2014.02.013_bib8","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1016\/j.ajhg.2008.03.018","article-title":"A defect in the TUSC3 gene is associated with autosomal recessive mental retardation","volume":"82","author":"Garshasbi","year":"2008","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.str.2014.02.013_bib9","doi-asserted-by":"crossref","first-page":"1976","DOI":"10.1002\/ajmg.a.34077","article-title":"A novel nonsense mutation in TUSC3 is responsible for non-syndromic autosomal recessive mental retardation in a consanguineous Iranian family","volume":"155A","author":"Garshasbi","year":"2011","journal-title":"Am. J. Med. Genet. A."},{"key":"10.1016\/j.str.2014.02.013_bib10","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.bbrc.2011.05.050","article-title":"Solution structure of a human minimembrane protein Ost4, a subunit of the oligosaccharyltransferase complex","volume":"409","author":"Gayen","year":"2011","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.str.2014.02.013_bib11","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1146\/annurev.biochem.73.011303.074043","article-title":"Role of glycosylation in development","volume":"73","author":"Haltiwanger","year":"2004","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.str.2014.02.013_bib12","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1091\/mbc.5.3.253","article-title":"How N-linked oligosaccharides affect glycoprotein folding in the endoplasmic reticulum","volume":"5","author":"Helenius","year":"1994","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.str.2014.02.013_bib13","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1146\/annurev.biochem.73.011303.073752","article-title":"Roles of N-linked glycans in the endoplasmic reticulum","volume":"73","author":"Helenius","year":"2004","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.str.2014.02.013_bib14","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1146\/annurev.bi.60.070191.003441","article-title":"Translational control in mammalian cells","volume":"60","author":"Hershey","year":"1991","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.str.2014.02.013_bib15","doi-asserted-by":"crossref","first-page":"32450","DOI":"10.1074\/jbc.M112.342253","article-title":"Eukaryotic N-glycosylation occurs via the membrane-anchored C-terminal domain of the Stt3p subunit of oligosaccharyltransferase","volume":"287","author":"Huang","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2014.02.013_bib16","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1126\/science.1523409","article-title":"Oxidized redox state of glutathione in the endoplasmic reticulum","volume":"257","author":"Hwang","year":"1992","journal-title":"Science"},{"key":"10.1016\/j.str.2014.02.013_bib17","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1002\/pro.610","article-title":"Polypeptide binding specificities of Saccharomyces cerevisiae oligosaccharyltransferase accessory proteins Ost3p and Ost6p","volume":"20","author":"Jamaluddin","year":"2011","journal-title":"Protein Sci."},{"key":"10.1016\/j.str.2014.02.013_bib18","doi-asserted-by":"crossref","first-page":"47R","DOI":"10.1093\/glycob\/cwj066","article-title":"An evolving view of the eukaryotic oligosaccharyltransferase","volume":"16","author":"Kelleher","year":"2006","journal-title":"Glycobiology"},{"key":"10.1016\/j.str.2014.02.013_bib19","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S1097-2765(03)00243-0","article-title":"Oligosaccharyltransferase isoforms that contain different catalytic STT3 subunits have distinct enzymatic properties","volume":"12","author":"Kelleher","year":"2003","journal-title":"Mol. Cell"},{"key":"10.1016\/j.str.2014.02.013_bib20","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1002\/prot.20921","article-title":"MUSTANG: a multiple structural alignment algorithm","volume":"64","author":"Konagurthu","year":"2006","journal-title":"Proteins"},{"key":"10.1016\/j.str.2014.02.013_bib21","doi-asserted-by":"crossref","first-page":"e10048","DOI":"10.1371\/journal.pone.0010048","article-title":"MUSTANG-MR structural sieving server: applications in protein structural analysis and crystallography","volume":"5","author":"Konagurthu","year":"2010","journal-title":"PLoS ONE"},{"key":"10.1016\/j.str.2014.02.013_bib22","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1038\/nature10246","article-title":"Second messenger role for Mg2+ revealed by human T-cell immunodeficiency","volume":"475","author":"Li","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.str.2014.02.013_bib23","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1038\/nature10151","article-title":"X-ray structure of a bacterial oligosaccharyltransferase","volume":"474","author":"Lizak","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.str.2014.02.013_bib24","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1006\/geno.1996.0322","article-title":"Structure and methylation-associated silencing of a gene within a homozygously deleted region of human chromosome band 8p22","volume":"35","author":"MacGrogan","year":"1996","journal-title":"Genomics"},{"key":"10.1016\/j.str.2014.02.013_bib25","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1007\/s10545-011-9337-1","article-title":"Oligosaccharyltransferase: the central enzyme of N-linked protein glycosylation","volume":"34","author":"Mohorko","year":"2011","journal-title":"J.\u00a0Inherit. Metab. Dis."},{"key":"10.1016\/j.str.2014.02.013_bib26","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1016\/j.ajhg.2008.03.021","article-title":"Oligosaccharyltransferase-subunit mutations in nonsyndromic mental retardation","volume":"82","author":"Molinari","year":"2008","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.str.2014.02.013_bib27","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1006\/jmbi.2000.4042","article-title":"T-Coffee: A novel method for fast and accurate multiple sequence alignment","volume":"302","author":"Notredame","year":"2000","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.str.2014.02.013_bib28","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/j.cell.2006.08.019","article-title":"Glycosylation in cellular mechanisms of health and disease","volume":"126","author":"Ohtsubo","year":"2006","journal-title":"Cell"},{"key":"10.1016\/j.str.2014.02.013_bib29","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1093\/glycob\/cwh008","article-title":"Statistical analysis of the protein environment of N-glycosylation sites: implications for occupancy, structure, and folding","volume":"14","author":"Petrescu","year":"2004","journal-title":"Glycobiology"},{"key":"10.1016\/j.str.2014.02.013_bib30","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1002\/cncr.27850","article-title":"Methylation status of TUSC3 is a prognostic factor in ovarian cancer","volume":"119","author":"Pils","year":"2013","journal-title":"Cancer"},{"key":"10.1016\/j.str.2014.02.013_bib31","doi-asserted-by":"crossref","first-page":"7944","DOI":"10.1073\/pnas.89.17.7944","article-title":"Equilibrium and kinetic constants for the thiol-disulfide interchange reaction between glutathione and dithiothreitol","volume":"89","author":"Rothwarf","year":"1992","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.str.2014.02.013_bib32","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.cell.2008.11.047","article-title":"Cotranslational and posttranslational N-glycosylation of polypeptides by distinct mammalian OST isoforms","volume":"136","author":"Ruiz-Canada","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.str.2014.02.013_bib33","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1074\/mcp.M800219-MCP200","article-title":"Analysis of glycosylation site occupancy reveals a role for Ost3p and Ost6p in site-specific N-glycosylation efficiency","volume":"8","author":"Schulz","year":"2009","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.str.2014.02.013_bib34","doi-asserted-by":"crossref","first-page":"11061","DOI":"10.1073\/pnas.0812515106","article-title":"Oxidoreductase activity of oligosaccharyltransferase subunits Ost3p and Ost6p defines site-specific glycosylation efficiency","volume":"106","author":"Schulz","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.str.2014.02.013_bib35","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/j.sbi.2011.08.005","article-title":"Mechanisms and principles of N-linked protein glycosylation","volume":"21","author":"Schwarz","year":"2011","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.str.2014.02.013_bib36","doi-asserted-by":"crossref","first-page":"6564","DOI":"10.1016\/j.febslet.2005.10.063","article-title":"Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit","volume":"579","author":"Schwarz","year":"2005","journal-title":"FEBS Lett."},{"key":"10.1016\/j.str.2014.02.013_bib37","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1083\/jcb.201301031","article-title":"Extreme C-terminal sites are posttranslocationally glycosylated by the STT3B isoform of the OST","volume":"201","author":"Shrimal","year":"2013","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.str.2014.02.013_bib38","doi-asserted-by":"crossref","first-page":"4638","DOI":"10.1093\/hmg\/ddt312","article-title":"Mutations in STT3A and STT3B cause two congenital disorders of glycosylation","volume":"22","author":"Shrimal","year":"2013","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.str.2014.02.013_bib39","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1002\/(SICI)1097-4644(19960616)61:4<562::AID-JCB9>3.0.CO;2-M","article-title":"Carbohydrate-carbohydrate interactions in adhesion","volume":"61","author":"Spillmann","year":"1996","journal-title":"J.\u00a0Cell. Biochem."},{"key":"10.1016\/j.str.2014.02.013_bib40","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.3892\/ijo.2013.1824","article-title":"Loss of the oligosaccharyl transferase subunit TUSC3 promotes proliferation and migration of ovarian cancer cells","volume":"42","author":"Va\u0148hara","year":"2013","journal-title":"Int. J. Oncol."},{"key":"10.1016\/j.str.2014.02.013_bib41","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1093\/glycob\/3.2.97","article-title":"Biological roles of oligosaccharides: all of the theories are correct","volume":"3","author":"Varki","year":"1993","journal-title":"Glycobiology"},{"key":"10.1016\/j.str.2014.02.013_bib42","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.bbrc.2009.10.047","article-title":"Screening for\u00a0OST deficiencies in unsolved CDG-I patients","volume":"390","author":"Vleugels","year":"2009","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.str.2014.02.013_bib43","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1242\/jcs.000729","article-title":"Ribophorin I acts as a substrate-specific facilitator of N-glycosylation","volume":"120","author":"Wilson","year":"2007","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.str.2014.02.013_bib44","doi-asserted-by":"crossref","first-page":"4195","DOI":"10.1074\/jbc.M410329200","article-title":"Ribophorin I associates with a subset of membrane proteins after their integration at the sec61 translocon","volume":"280","author":"Wilson","year":"2005","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2014.02.013_bib45","doi-asserted-by":"crossref","first-page":"9534","DOI":"10.1073\/pnas.0711846105","article-title":"Ribophorin I regulates substrate delivery to the oligosaccharyltransferase core","volume":"105","author":"Wilson","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.str.2014.02.013_bib46","doi-asserted-by":"crossref","first-page":"15750","DOI":"10.1073\/pnas.0908332106","article-title":"Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebrate embryonic development","volume":"106","author":"Zhou","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.str.2014.02.013_bib47","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/j.cell.2010.04.012","article-title":"Precision mapping of an in\u00a0vivo N-glycoproteome reveals rigid topological and sequence constraints","volume":"141","author":"Zielinska","year":"2010","journal-title":"Cell"}],"container-title":["Structure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969212614000537?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969212614000537?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,8]],"date-time":"2019-08-08T20:11:36Z","timestamp":1565295096000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0969212614000537"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":47,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["S0969212614000537"],"URL":"https:\/\/doi.org\/10.1016\/j.str.2014.02.013","relation":{},"ISSN":["0969-2126"],"issn-type":[{"value":"0969-2126","type":"print"}],"subject":[],"published":{"date-parts":[[2014,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural Basis of Substrate Specificity of Human Oligosaccharyl Transferase Subunit N33\/Tusc3 and Its Role in Regulating Protein N-Glycosylation","name":"articletitle","label":"Article Title"},{"value":"Structure","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.str.2014.02.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}