{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:02:00Z","timestamp":1790208120678,"version":"4.1.0"},"reference-count":36,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,5,2]],"date-time":"2018-05-02T00:00:00Z","timestamp":1525219200000},"content-version":"vor","delay-in-days":366,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"CIHR","doi-asserted-by":"publisher","award":["MOP-13107"],"award-info":[{"award-number":["MOP-13107"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Structure"],"published-print":{"date-parts":[[2017,5]]},"DOI":"10.1016\/j.str.2017.03.007","type":"journal-article","created":{"date-parts":[[2017,4,13]],"date-time":"2017-04-13T16:46:30Z","timestamp":1492101990000},"page":"750-761.e5","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":37,"title":["Structural Basis for Kinase-Mediated Macrolide Antibiotic Resistance"],"prefix":"10.1016","volume":"25","author":[{"given":"Desiree H.","family":"Fong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Burk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Blanchet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy Y.","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert M.","family":"Berghuis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.str.2017.03.007_bib1","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/S1367-5931(02)00357-5","article-title":"A strategy for the design of multiplex inhibitors for kinase-mediated signalling in angiogenesis","volume":"6","author":"Adams","year":"2002","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.str.2017.03.007_bib2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2017.03.007_bib3","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1107\/S0907444912001308","article-title":"Towards automated crystallographic structure refinement with phenix.refine","volume":"68","author":"Afonine","year":"2012","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2017.03.007_bib4","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1021\/acschembio.5b01070","article-title":"Structural analysis of the tobramycin and gentamicin clinical resistome reveals limitations for next-generation aminoglycoside design","volume":"11","author":"Bassenden","year":"2016","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.str.2017.03.007_bib5","doi-asserted-by":"crossref","first-page":"17158","DOI":"10.1073\/pnas.1008685107","article-title":"From the Cover: revisiting the structures of several antibiotics bound to the bacterial ribosome","volume":"107","author":"Bulkley","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.str.2017.03.007_bib6","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/j.str.2016.04.002","article-title":"Antibiotic binding drives\u00a0catalytic activation of aminoglycoside kinase APH(2\u2033)-Ia","volume":"24","author":"Caldwell","year":"2016","journal-title":"Structure"},{"key":"10.1016\/j.str.2017.03.007_bib7","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1107\/S0907444909042073","article-title":"MolProbity: all-atom structure validation for macromolecular crystallography","volume":"66","author":"Chen","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2017.03.007_bib8","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1111\/j.1574-6968.2007.00643.x","article-title":"Resistance phenotypes conferred by macrolide phosphotransferases","volume":"269","author":"Chesneau","year":"2007","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.str.2017.03.007_bib9","doi-asserted-by":"crossref","first-page":"17152","DOI":"10.1073\/pnas.1007988107","article-title":"Structures of the\u00a0Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action","volume":"107","author":"Dunkle","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.str.2017.03.007_bib10","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of Coot","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2017.03.007_bib11","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.1093\/emboj\/21.10.2323","article-title":"Substrate promiscuity of an aminoglycoside antibiotic resistance enzyme via target mimicry","volume":"21","author":"Fong","year":"2002","journal-title":"EMBO J."},{"key":"10.1016\/j.str.2017.03.007_bib12","doi-asserted-by":"crossref","first-page":"1966","DOI":"10.1107\/S0907444903018043","article-title":"Substructure search procedures for macromolecular structures","volume":"59","author":"Grosse-Kunstleve","year":"2003","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2017.03.007_bib13","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1038\/nrd3267","article-title":"The antibiotics market","volume":"9","author":"Hamad","year":"2010","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.str.2017.03.007_bib14","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S1097-2765(02)00570-1","article-title":"The structures of four macrolide antibiotics bound to the large ribosomal subunit","volume":"10","author":"Hansen","year":"2002","journal-title":"Mol. Cell"},{"key":"10.1016\/j.str.2017.03.007_bib15","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","article-title":"XDS","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2017.03.007_bib16","first-page":"89","article-title":"Purification and characterization of macrolide 2\u2032-phosphotransferase type II from a strain of Escherichia coli highly resistant to macrolide antibiotics","volume":"76","author":"Kono","year":"1992","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.str.2017.03.007_bib17","doi-asserted-by":"crossref","first-page":"10388","DOI":"10.1073\/pnas.0701809104","article-title":"Structural basis for streptogramin B resistance in Staphylococcus aureus by virginiamycin B lyase","volume":"104","author":"Korczynska","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.str.2017.03.007_bib18","doi-asserted-by":"crossref","first-page":"W590","DOI":"10.1093\/nar\/gkh477","article-title":"SuperPose: a simple server for sophisticated structural superposition","volume":"32","author":"Maiti","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.str.2017.03.007_bib19","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser crystallographic software","volume":"40","author":"McCoy","year":"2007","journal-title":"J.\u00a0Appl. Crystallogr."},{"key":"10.1016\/j.str.2017.03.007_bib20","first-page":"197","article-title":"Cloning and nucleotide sequence of the mphB gene for macrolide 2\u2032-phosphotransferase II in Escherichia coli","volume":"144","author":"Noguchi","year":"1996","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.str.2017.03.007_bib21","doi-asserted-by":"crossref","first-page":"1354","DOI":"10.1128\/AAC.33.8.1354","article-title":"Purification and characterization of macrolide 2\u2032-phosphotransferase from a strain of Escherichia coli that is highly resistant to erythromycin","volume":"33","author":"O'Hara","year":"1989","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.str.2017.03.007_bib22","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","article-title":"Processing of X-ray diffraction data collected in oscillation mode","volume":"276","author":"Otwinowski","year":"1997","journal-title":"Meth. Enzymol."},{"key":"10.1016\/j.str.2017.03.007_bib23","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1107\/S0021889805038987","article-title":"TLSMD web server for the generation of multi-group TLS models","volume":"39","author":"Painter","year":"2006","journal-title":"J.\u00a0Appl. Crystallogr."},{"key":"10.1016\/j.str.2017.03.007_bib24","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.3201\/eid1510.090696","article-title":"Escherichia coli as reservoir for macrolide resistance genes","volume":"15","author":"Phuc Nguyen","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.str.2017.03.007_bib25","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1111\/j.1574-6968.2008.01145.x","article-title":"Update on macrolide-lincosamide-streptogramin, ketolide, and oxazolidinone resistance genes","volume":"282","author":"Roberts","year":"2008","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.str.2017.03.007_bib26","series-title":"The PyMOL Molecular Graphics System, Version 1.8","author":"Schrodinger, LLC","year":"2015"},{"key":"10.1016\/j.str.2017.03.007_bib27","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1128\/AAC.01570-09","article-title":"Nucleotide selectivity of antibiotic kinases","volume":"54","author":"Shakya","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.str.2017.03.007_bib28","doi-asserted-by":"crossref","first-page":"13094","DOI":"10.1074\/jbc.M112.349670","article-title":"Structural basis for dual nucleotide selectivity of aminoglycoside 2\u2033-phosphotransferase IVa provides insight on determinants of nucleotide specificity of aminoglycoside kinases","volume":"287","author":"Shi","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2017.03.007_bib29","doi-asserted-by":"crossref","first-page":"9","DOI":"10.18388\/abp.1996_4561","article-title":"The NTP phosphate donor in kinase reactions: is ATP a monopolist?","volume":"43","author":"Shugar","year":"1996","journal-title":"Acta Biochim. Pol."},{"key":"10.1016\/j.str.2017.03.007_bib30","doi-asserted-by":"crossref","first-page":"12893","DOI":"10.1074\/jbc.M112.341206","article-title":"Aminoglycoside-2\u2033 phosphotransferase-IIIa (APH(2\u2033)-IIIa) prefers GTP over ATP: structural templates for nucleotide recognition in the bacterial aminoglycoside-2\u2033 kinases","volume":"287","author":"Smith","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2017.03.007_bib31","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.pep.2005.01.016","article-title":"Protein production by auto-induction in high density shaking cultures","volume":"41","author":"Studier","year":"2005","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.str.2017.03.007_bib32","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1107\/S0907444906017161","article-title":"Automated ligand fitting by core-fragment fitting and extension into density","volume":"62","author":"Terwilliger","year":"2006","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2017.03.007_bib33","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1107\/S090744490705024X","article-title":"Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard","volume":"64","author":"Terwilliger","year":"2008","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2017.03.007_bib34","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1107\/S0907444909012098","article-title":"Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard","volume":"65","author":"Terwilliger","year":"2009","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2017.03.007_bib35","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.cell.2005.02.005","article-title":"Structures of MLSBK antibiotics bound to mutated large ribosomal subunits provide a structural explanation for resistance","volume":"121","author":"Tu","year":"2005","journal-title":"Cell"},{"key":"10.1016\/j.str.2017.03.007_bib36","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1016\/j.jmb.2009.02.058","article-title":"Structure and function of the macrolide biosensor protein, MphR(A), with and without erythromycin","volume":"387","author":"Zheng","year":"2009","journal-title":"J.\u00a0Mol. Biol."}],"container-title":["Structure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969212617300679?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969212617300679?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,10,5]],"date-time":"2020-10-05T13:17:00Z","timestamp":1601903820000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0969212617300679"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":36,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["S0969212617300679"],"URL":"https:\/\/doi.org\/10.1016\/j.str.2017.03.007","relation":{},"ISSN":["0969-2126"],"issn-type":[{"value":"0969-2126","type":"print"}],"subject":[],"published":{"date-parts":[[2017,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural Basis for Kinase-Mediated Macrolide Antibiotic Resistance","name":"articletitle","label":"Article Title"},{"value":"Structure","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.str.2017.03.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}