{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T15:20:19Z","timestamp":1790522419110,"version":"4.1.0"},"reference-count":82,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T00:00:00Z","timestamp":1593648000000},"content-version":"vor","delay-in-days":367,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Structure"],"published-print":{"date-parts":[[2019,7]]},"DOI":"10.1016\/j.str.2019.03.021","type":"journal-article","created":{"date-parts":[[2019,5,2]],"date-time":"2019-05-02T10:32:04Z","timestamp":1556793124000},"page":"1082-1093.e5","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":46,"title":["Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus"],"prefix":"10.1016","volume":"27","author":[{"given":"Benjamin","family":"Bardiaux","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisele Cardoso","family":"de Amorim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Areli","family":"Luna Rico","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weili","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Guilvout","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Jollivet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Nilges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward H.","family":"Egelman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Izadi-Pruneyre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivera","family":"Francetic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.str.2019.03.021_bib1","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/j.1365-2958.2006.05482.x","article-title":"Substitutions in the N-terminal alpha helical spine of Neisseria gonorrhoeae pilin affect type IV pilus assembly, dynamics and associated functions","volume":"63","author":"Aas","year":"2007","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.str.2019.03.021_bib2","first-page":"36","article-title":"Image processing with ImageJ","volume":"11","author":"Abramoff","year":"2004","journal-title":"Biophotonics Internatl"},{"key":"10.1016\/j.str.2019.03.021_bib3","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2019.03.021_bib4","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.1093\/bioinformatics\/btu336","article-title":"Evol and ProDy for bridging protein sequence evolution and structural dynamics","volume":"30","author":"Bakan","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.str.2019.03.021_bib5","doi-asserted-by":"crossref","first-page":"10037","DOI":"10.1073\/pnas.181342398","article-title":"Electrostatics of nanosystems: application to microtubules and the ribosome","volume":"98","author":"Baker","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2019.03.021_bib6","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1002\/prot.22268","article-title":"Influence of different assignment conditions on the determination of symmetric homodimeric structures with ARIA","volume":"75","author":"Bardiaux","year":"2008","journal-title":"Proteins"},{"key":"10.1016\/j.str.2019.03.021_bib7","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/0378-1119(91)90541-I","article-title":"Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives","volume":"102","author":"Bartolome","year":"1991","journal-title":"Gene"},{"key":"10.1016\/j.str.2019.03.021_bib8","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1002\/prot.22980","article-title":"Bayesian estimation of NMR restraint potential and weight: a validation on a representative set of protein structures","volume":"79","author":"Bernard","year":"2011","journal-title":"Proteins"},{"key":"10.1016\/j.str.2019.03.021_bib9","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1093\/femsre\/fuu001","article-title":"Exceptionally widespread nanomachines composed of type IV pilins: the prokaryotic Swiss Army knives","volume":"39","author":"Berry","year":"2015","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.str.2019.03.021_bib10","doi-asserted-by":"crossref","first-page":"2728","DOI":"10.1038\/nprot.2007.406","article-title":"Version 1.2 of the crystallography and NMR system","volume":"2","author":"Brunger","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.str.2019.03.021_bib11","doi-asserted-by":"crossref","first-page":"13081","DOI":"10.1073\/pnas.1001703107","article-title":"Detailed structural and assembly model of the type II secretion pilus from sparse data","volume":"107","author":"Campos","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2019.03.021_bib12","doi-asserted-by":"crossref","first-page":"3065","DOI":"10.1073\/pnas.1218832110","article-title":"Specific DNA recognition mediated by a type IV pilin","volume":"110","author":"Cehovin","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2019.03.021_bib13","doi-asserted-by":"crossref","first-page":"4817","DOI":"10.1016\/j.vaccine.2010.04.065","article-title":"Sequence conservation of pilus subunits in Neisseria meningitidis","volume":"28","author":"Cehovin","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.str.2019.03.021_bib14","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1111\/mmi.12033","article-title":"Heterologous assembly of type IV pili by a type II secretion system reveals the role of minor pilins in assembly initiation","volume":"86","author":"Cisneros","year":"2012","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.str.2019.03.021_bib15","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/j.bpj.2008.10.017","article-title":"Dynamics of type IV pili is controlled by switching between multiple states","volume":"96","author":"Clausen","year":"2009","journal-title":"Biophys. J."},{"key":"10.1016\/j.str.2019.03.021_bib16","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/j.molcel.2006.07.004","article-title":"Type IV pilus structure by cryo-electron microscopy and crystallography: implications for pilus assembly and functions","volume":"23","author":"Craig","year":"2006","journal-title":"Mol. Cell"},{"key":"10.1016\/j.str.2019.03.021_bib17","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products","volume":"97","author":"Datsenko","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2019.03.021_bib18","doi-asserted-by":"crossref","first-page":"7060","DOI":"10.1128\/JB.182.24.7060-7066.2000","article-title":"Sensitive genetic screen for protease activity based on a cyclic AMP signaling cascade in Escherichia coli","volume":"182","author":"Dautin","year":"2000","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.str.2019.03.021_bib19","doi-asserted-by":"crossref","first-page":"W375","DOI":"10.1093\/nar\/gkm216","article-title":"MolProbity: all-atom contacts and structure validation for proteins and nucleic acids","volume":"35","author":"Davis","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.str.2019.03.021_bib20","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s12104-012-9449-z","article-title":"1H, 15N and 13C resonance assignments of PpdD, a type IV pilin from enterohemorrhagic Escherichia coli","volume":"8","author":"de Amorim","year":"2014","journal-title":"Biomol. NMR Assign."},{"key":"10.1016\/j.str.2019.03.021_bib21","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/BF00197809","article-title":"NMRPipe: a multidimensional spectral processing system based on UNIX pipes","volume":"6","author":"Delaglio","year":"1995","journal-title":"J.\u00a0Biomol. NMR"},{"key":"10.1016\/j.str.2019.03.021_bib22","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0304-3991(00)00062-0","article-title":"A robust algorithm for the reconstruction of helical filaments using single-particle methods","volume":"85","author":"Egelman","year":"2000","journal-title":"Ultramicroscopy"},{"key":"10.1016\/j.str.2019.03.021_bib23","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1038\/s41564-018-0174-y","article-title":"Retraction of DNA-bound type IV competence pili initiates DNA uptake during natural transformation in Vibrio cholerae","volume":"3","author":"Ellison","year":"2018","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.str.2019.03.021_bib24","doi-asserted-by":"crossref","first-page":"7045","DOI":"10.1128\/JB.187.20.7045-7055.2005","article-title":"Towards the identification of type II secretion signals in a nonacylated variant of pullulanase from Klebsiella oxytoca","volume":"187","author":"Franceti\u0107","year":"2005","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.str.2019.03.021_bib25","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1006\/jsbi.1996.0030","article-title":"SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields","volume":"116","author":"Frank","year":"1996","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.str.2019.03.021_bib26","doi-asserted-by":"crossref","first-page":"44254","DOI":"10.1074\/jbc.M111.297242","article-title":"Ultrahigh resolution and full-length pilin structures with insights for filament assembly, pathogenic functions, and vaccine potential","volume":"286","author":"Hartung","year":"2011","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2019.03.021_bib27","series-title":"NACCESS Computer Program. Department of Biochemistry and Molecular Biology","author":"Hubbard","year":"1993"},{"key":"10.1016\/j.str.2019.03.021_bib28","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.15252\/embj.201488031","article-title":"The number of Neisseria meningitidis type IV pili determines host cell interaction","volume":"33","author":"Imhaus","year":"2014","journal-title":"EMBO J."},{"key":"10.1016\/j.str.2019.03.021_bib29","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1006\/jmbi.1999.3091","article-title":"Protein secondary structure prediction based on position-specific scoring matrices","volume":"292","author":"Jones","year":"1999","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.str.2019.03.021_bib30","doi-asserted-by":"crossref","first-page":"15674","DOI":"10.1073\/pnas.1314045110","article-title":"Assessing the utility of coevolution-based residue-residue contact predictions in a sequence- and structure-rich era","volume":"110","author":"Kamisetty","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2019.03.021_bib31","doi-asserted-by":"crossref","first-page":"5752","DOI":"10.1073\/pnas.95.10.5752","article-title":"A bacterial two-hybrid system based on a reconstituted signal transduction pathway","volume":"95","author":"Karimova","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2019.03.021_bib32","doi-asserted-by":"crossref","first-page":"7031","DOI":"10.1128\/AEM.05138-11","article-title":"Cell surface attachment structures contribute to biofilm formation and xylem colonization by Erwinia amylovora","volume":"77","author":"Koczan","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.str.2019.03.021_bib33","doi-asserted-by":"crossref","first-page":"13015","DOI":"10.1038\/ncomms13015","article-title":"Structure of the Neisseria meningitidis type IV pilus","volume":"7","author":"Kolappan","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.str.2019.03.021_bib34","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1002\/prot.22488","article-title":"Improved prediction of protein side-chain conformations with SCWRL4","volume":"77","author":"Krivov","year":"2009","journal-title":"Proteins"},{"key":"10.1016\/j.str.2019.03.021_bib35","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1107\/S0021889892009944","article-title":"PROCHECK: a program to check the stereochemical quality of protein structures","volume":"26","author":"Laskowski","year":"1993","journal-title":"J.\u00a0Appl. Cryst."},{"key":"10.1016\/j.str.2019.03.021_bib36","doi-asserted-by":"crossref","first-page":"e12127","DOI":"10.1371\/journal.pone.0012127","article-title":"The hemorrhagic coli pilus (HCP) of Escherichia coli O157:H7 is an inducer of proinflammatory cytokine secretion in intestinal epithelial cells","volume":"5","author":"Ledesma","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.str.2019.03.021_bib37","doi-asserted-by":"crossref","first-page":"2229","DOI":"10.1128\/JB.00220-15","article-title":"Novel role for PilNO in type IV pilus retraction revealed by alignment subcomplex mutations","volume":"197","author":"Leighton","year":"2015","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.str.2019.03.021_bib38","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1002\/prot.10299","article-title":"Refinement of protein structures in explicit solvent","volume":"50","author":"Linge","year":"2003","journal-title":"Proteins"},{"key":"10.1016\/j.str.2019.03.021_bib39","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.1038\/s41564-017-0041-2","article-title":"Structure of the calcium-dependent type 2 secretion pseudopilus","volume":"2","author":"Lopez-Castilla","year":"2017","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.str.2019.03.021_bib40","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/978-1-4939-7759-8_18","article-title":"Analysis of bacterial pilus assembly by shearing and immunofluorescence microscopy","volume":"1764","author":"Luna Rico","year":"2018","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.str.2019.03.021_bib41","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1111\/mmi.14188","article-title":"Functional reconstitution of the type IVa assembly system from enterohemorrhagic Escherichia coli","volume":"111","author":"Luna Rico","year":"2019","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.str.2019.03.021_bib42","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.tim.2015.10.008","article-title":"The DNA-uptake process of naturally competent Vibrio cholerae","volume":"24","author":"Matthey","year":"2016","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.str.2019.03.021_bib43","doi-asserted-by":"crossref","first-page":"15091","DOI":"10.1038\/ncomms15091","article-title":"The molecular mechanism of the type IVa pilus motors","volume":"8","author":"McCallum","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.str.2019.03.021_bib44","series-title":"Experiments in Molecular Genetics","author":"Miller","year":"1972"},{"key":"10.1016\/j.str.2019.03.021_bib45","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/S1047-8477(03)00069-8","article-title":"Accurate determination of local defocus and specimen tilt in electron microscopy","volume":"142","author":"Mindell","year":"2003","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.str.2019.03.021_bib46","doi-asserted-by":"crossref","DOI":"10.1093\/femsle\/fnw179","article-title":"A secretome view of colonisation factors in Shiga toxin-encoding Escherichia coli (STEC): from enterohaemorrhagic E.\u00a0coli (EHEC) to related enteropathotypes","volume":"363","author":"Monteiro","year":"2016","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.str.2019.03.021_bib47","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1021\/acsinfecdis.7b00229","article-title":"A highly dynamic loop of the Pseudomonas aeruginosa PA14 type IV pilin is essential for pilus assembly","volume":"4","author":"Nguyen","year":"2018","journal-title":"ACS Infect. Dis."},{"key":"10.1016\/j.str.2019.03.021_bib48","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1016\/j.str.2008.07.008","article-title":"Accurate NMR structures through minimization of an extended hybrid energy","volume":"16","author":"Nilges","year":"2008","journal-title":"Structure"},{"key":"10.1016\/j.str.2019.03.021_bib49","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.str.2014.03.001","article-title":"Distinct docking and stabilization steps of the pseudopilus conformational transition path suggest rotational assembly of type IV pilus-like fibers","volume":"22","author":"Nivaskumar","year":"2014","journal-title":"Structure"},{"key":"10.1016\/j.str.2019.03.021_bib50","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1111\/mmi.13432","article-title":"Pseudopilin residue E5 is essential for recruitment by the type 2 secretion system assembly platform","volume":"101","author":"Nivaskumar","year":"2016","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.str.2019.03.021_bib51","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1006\/jmre.1998.1361","article-title":"Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra","volume":"131","author":"Ottiger","year":"1998","journal-title":"J.\u00a0Magn. Reson."},{"key":"10.1016\/j.str.2019.03.021_bib52","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1111\/j.1365-2958.2008.06197.x","article-title":"Type IV pili: e pluribus unum?","volume":"68","author":"Pelicic","year":"2008","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.str.2019.03.021_bib53","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera - a visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.str.2019.03.021_bib54","doi-asserted-by":"crossref","first-page":"2142","DOI":"10.1128\/JB.182.8.2142-2152.2000","article-title":"Multiple interactions between pullulanase secreton components involved in stabilization and cytoplasmic membrane association of PulE","volume":"182","author":"Possot","year":"2000","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.str.2019.03.021_bib55","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/nmeth.1818","article-title":"HHblits: lightning-fast iterative protein sequence searching by HMM-HMM alignment","volume":"9","author":"Remmert","year":"2011","journal-title":"Nat. Methods"},{"key":"10.1016\/j.str.2019.03.021_bib56","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1093\/bioinformatics\/btl589","article-title":"ARIA2: automated NOE assignment and data integration in NMR structure calculation","volume":"23","author":"Rieping","year":"2007","journal-title":"Bioinformatics"},{"key":"10.1016\/j.str.2019.03.021_bib57","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1074\/jbc.RA118.005814","article-title":"The structure of PilA from Acinetobacter baumannii AB5075 suggests a mechanism for functional specialization in Acinetobacter type IV pili","volume":"294","author":"Ronish","year":"2019","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2019.03.021_bib58","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1006\/jmbi.1993.1626","article-title":"Comparative protein modelling by satisfaction of spatial restraints","volume":"234","author":"Sali","year":"1993","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.str.2019.03.021_bib59","doi-asserted-by":"crossref","first-page":"1746","DOI":"10.1016\/j.jmb.2017.04.005","article-title":"Polar N-terminal residues conserved in type 2 secretion pseudopilins determine subunit targeting and membrane extraction during fibre assembly","volume":"429","author":"Santos-Moreno","year":"2017","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.str.2019.03.021_bib60","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1128\/JB.182.3.848-854.2000","article-title":"PpdD type IV pilin of Escherichia coli K-12 can be assembled into pili in Pseudomonas aeruginosa","volume":"182","author":"Sauvonnet","year":"2000","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.str.2019.03.021_bib61","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/0003-2697(87)90587-2","article-title":"Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100\u00a0kDa","volume":"166","author":"Schagger","year":"1987","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.str.2019.03.021_bib62","series-title":"The PyMOL Molecular Graphics System, Version 1.8","author":"Schr\u00f6dinger","year":"2015"},{"key":"10.1016\/j.str.2019.03.021_bib63","doi-asserted-by":"crossref","first-page":"3128","DOI":"10.1093\/bioinformatics\/btu500","article-title":"CCMpred\u2013fast and precise prediction of protein residue-residue contacts from correlated mutations","volume":"30","author":"Seemayer","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.str.2019.03.021_bib64","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1016\/j.str.2011.03.019","article-title":"A smoothed backbone-dependent rotamer library for proteins derived from adaptive kernel density estimates and regressions","volume":"19","author":"Shapovalov","year":"2011","journal-title":"Structure"},{"key":"10.1016\/j.str.2019.03.021_bib65","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s10858-009-9333-z","article-title":"TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts","volume":"44","author":"Shen","year":"2009","journal-title":"J.\u00a0Biomol. NMR"},{"key":"10.1016\/j.str.2019.03.021_bib66","doi-asserted-by":"crossref","first-page":"5180","DOI":"10.1128\/JB.00476-09","article-title":"Sxy induces a CRP-S regulon in Escherichia coli","volume":"191","author":"Sinha","year":"2009","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.str.2019.03.021_bib67","doi-asserted-by":"crossref","first-page":"e35620","DOI":"10.1371\/journal.pone.0035620","article-title":"Natural DNA uptake by Escherichia coli","volume":"7","author":"Sinha","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.str.2019.03.021_bib68","doi-asserted-by":"crossref","first-page":"1656","DOI":"10.1016\/S0021-9258(18)52345-0","article-title":"Amino acid substitutions in pilin of Pseudomonas aeruginosa. Effect on leader peptide cleavage, amino-terminal methylation, and pilus assembly","volume":"266","author":"Strom","year":"1991","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2019.03.021_bib69","doi-asserted-by":"crossref","first-page":"3985","DOI":"10.1021\/bi002524h","article-title":"Backbone dynamics of receptor binding and antigenic regions of a Pseudomonas aeruginosa pilin monomer","volume":"40","author":"Suh","year":"2001","journal-title":"Biochemistry"},{"key":"10.1016\/j.str.2019.03.021_bib70","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jsb.2006.05.009","article-title":"EMAN2: an extensible image processing suite for electron microscopy","volume":"157","author":"Tang","year":"2007","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.str.2019.03.021_bib71","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.cell.2016.09.010","article-title":"Structured states of disordered proteins from genomic sequences","volume":"167","author":"Toth-Petroczy","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.str.2019.03.021_bib72","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1128\/JB.187.4.1455-1464.2005","article-title":"DNA binding: a novel function of Pseudomonas aeruginosa type IV pili","volume":"187","author":"van Schaik","year":"2005","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.str.2019.03.021_bib73","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1002\/prot.20449","article-title":"The CCPN data model for NMR spectroscopy: development of a software pipeline","volume":"59","author":"Vranken","year":"2005","journal-title":"Proteins"},{"key":"10.1016\/j.str.2019.03.021_bib82","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/0263-7855(90)80070-V","article-title":"WHAT IF: A molecular modeling and drug design program","volume":"8","author":"Vriend","year":"1990","journal-title":"J. Mol. Graph."},{"key":"10.1016\/j.str.2019.03.021_bib74","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.str.2017.07.016","article-title":"Cryoelectron microscopy reconstructions of the Pseudomonas aeruginosa and Neisseria gonorrhoeae type IV pili at sub-nanometer resolution","volume":"25","author":"Wang","year":"2017","journal-title":"Structure"},{"key":"10.1016\/j.str.2019.03.021_bib75","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/BF00211777","article-title":"1H, 13C and 15N chemical shift referencing in biomolecular NMR","volume":"6","author":"Wishart","year":"1995","journal-title":"J.\u00a0Biomol. NMR"},{"issue":"Pt 4","key":"10.1016\/j.str.2019.03.021_bib76","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1107\/S0907444911049791","article-title":"Conventions and workflows for using Situs","volume":"68","author":"Wriggers","year":"2012","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2019.03.021_bib77","doi-asserted-by":"crossref","first-page":"3519","DOI":"10.1172\/JCI30727","article-title":"Intestinal adherence associated with type IV pili of enterohemorrhagic Escherichia coli O157:H7","volume":"117","author":"Xicohtencatl-Cortes","year":"2007","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.str.2019.03.021_bib78","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1128\/JB.01306-08","article-title":"The type 4 pili of enterohemorrhagic Escherichia coli O157:H7 are multipurpose structures with pathogenic attributes","volume":"191","author":"Xicohtencatl-Cortes","year":"2009","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.str.2019.03.021_bib79","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0378-1119(85)90120-9","article-title":"Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors","volume":"33","author":"Yanisch-Perron","year":"1985","journal-title":"Gene"},{"key":"10.1016\/j.str.2019.03.021_bib80","doi-asserted-by":"crossref","first-page":"2302","DOI":"10.1093\/nar\/gki524","article-title":"TM-align: a protein structure alignment algorithm based on the TM-score","volume":"33","author":"Zhang","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.str.2019.03.021_bib81","doi-asserted-by":"crossref","first-page":"3791","DOI":"10.1021\/ja0000908","article-title":"Prediction of sterically induced alignment in a dilute liquid crystalline phase: aid to protein structure determination by NMR","volume":"122","author":"Zweckstetter","year":"2000","journal-title":"J.\u00a0Am. Chem. Soc."}],"container-title":["Structure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969212619301170?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969212619301170?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T02:33:53Z","timestamp":1760841233000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0969212619301170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7]]},"references-count":82,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["S0969212619301170"],"URL":"https:\/\/doi.org\/10.1016\/j.str.2019.03.021","relation":{},"ISSN":["0969-2126"],"issn-type":[{"value":"0969-2126","type":"print"}],"subject":[],"published":{"date-parts":[[2019,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus","name":"articletitle","label":"Article Title"},{"value":"Structure","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.str.2019.03.021","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}