{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T08:40:41Z","timestamp":1784882441978,"version":"3.55.0"},"reference-count":47,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T00:00:00Z","timestamp":1628035200000},"content-version":"vor","delay-in-days":368,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P30402"],"award-info":[{"award-number":["P30402"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["T855"],"award-info":[{"award-number":["T855"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["FWF \\u2013 P30402"],"award-info":[{"award-number":["FWF \\u2013 P30402"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["FWF \\u2013 T855"],"award-info":[{"award-number":["FWF \\u2013 T855"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010474","name":"RYR-1 Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010474","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT159601"],"award-info":[{"award-number":["PJT159601"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["CIHR \\u2013 PJT159601"],"award-info":[{"award-number":["CIHR \\u2013 PJT159601"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Structure"],"published-print":{"date-parts":[[2020,8]]},"DOI":"10.1016\/j.str.2020.05.005","type":"journal-article","created":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T10:35:38Z","timestamp":1591094138000},"page":"922-932.e5","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":29,"title":["Multiple Sequence Variants in STAC3 Affect Interactions with CaV1.1 and Excitation-Contraction Coupling"],"prefix":"10.1016","volume":"28","author":[{"given":"Britany","family":"Rufenach","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darren","family":"Christy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard E.","family":"Flucher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer M.","family":"Bui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Gsponer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Campiglio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"Van Petegem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.str.2020.05.005_bib1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2020.05.005_bib2","doi-asserted-by":"crossref","first-page":"2384","DOI":"10.1063\/1.439486","article-title":"Molecular dynamics simulations at constant pressure and-or temperature","volume":"72","author":"Andersen","year":"1980","journal-title":"J.\u00a0Chem. Phys."},{"key":"10.1016\/j.str.2020.05.005_bib3","doi-asserted-by":"crossref","first-page":"2587","DOI":"10.1083\/jcb.107.6.2587","article-title":"Structural evidence for direct interaction between the molecular components of the transverse tubule\/sarcoplasmic reticulum junction in skeletal muscle","volume":"107","author":"Block","year":"1988","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.str.2020.05.005_bib4","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1016\/j.str.2014.06.002","article-title":"Phosphorylation of an intrinsically disordered segment in Ets1 shifts conformational sampling toward binding-competent substates","volume":"22","author":"Bui","year":"2014","journal-title":"Structure"},{"key":"10.1016\/j.str.2020.05.005_bib5","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1073\/pnas.1715997115","article-title":"STAC proteins associate to the IQ domain of CaV1.2 and inhibit calcium-dependent inactivation","volume":"115","author":"Campiglio","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2020.05.005_bib6","doi-asserted-by":"crossref","first-page":"41003","DOI":"10.1038\/srep41003","article-title":"STAC3 stably interacts through its C1 domain with CaV1.1 in skeletal muscle triads","volume":"7","author":"Campiglio","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.str.2020.05.005_bib7","doi-asserted-by":"crossref","first-page":"9045","DOI":"10.1002\/jcp.26767","article-title":"STAC3 incorporation into skeletal muscle triads occurs independent of the dihydropyridine receptor","volume":"233","author":"Campiglio","year":"2018","journal-title":"J.\u00a0Cell. Physiol."},{"key":"10.1016\/j.str.2020.05.005_bib8","series-title":"AMBER 2018","author":"Case","year":"2018"},{"key":"10.1016\/j.str.2020.05.005_bib9","doi-asserted-by":"crossref","first-page":"10089","DOI":"10.1063\/1.464397","article-title":"Particle Mesh Ewald - an N.Log(N) method for Ewald Sums in large systems","volume":"98","author":"Darden","year":"1993","journal-title":"J.\u00a0Chem. Phys."},{"key":"10.1016\/j.str.2020.05.005_bib10","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1038\/s41467-017-00629-x","article-title":"The Ca(2+) influx through the mammalian skeletal muscle dihydropyridine receptor is irrelevant for muscle performance","volume":"8","author":"Dayal","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.str.2020.05.005_bib11","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1021\/bi00859a010","article-title":"Spectroscopic determination of tryptophan and tyrosine in proteins","volume":"6","author":"Edelhoch","year":"1967","journal-title":"Biochemistry"},{"key":"10.1016\/j.str.2020.05.005_bib12","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of Coot","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2020.05.005_bib13","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1126\/science.1862346","article-title":"Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia","volume":"253","author":"Fujii","year":"1991","journal-title":"Science"},{"key":"10.1016\/j.str.2020.05.005_bib14","doi-asserted-by":"crossref","first-page":"4483","DOI":"10.1038\/s41598-018-22713-y","article-title":"The voltage-gated sodium channel EF-hands form an interaction with the III-IV linker that is disturbed by disease-causing mutations","volume":"8","author":"Gardill","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.str.2020.05.005_bib15","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1073\/pnas.95.4.1903","article-title":"Tagging with green fluorescent protein reveals a distinct subcellular distribution of L-type and non-L-type Ca2+ channels expressed in dysgenic myotubes","volume":"95","author":"Grabner","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2020.05.005_bib16","doi-asserted-by":"crossref","first-page":"21913","DOI":"10.1074\/jbc.274.31.21913","article-title":"The II-III loop of the skeletal muscle dihydropyridine receptor is responsible for the Bi-directional coupling with the ryanodine receptor","volume":"274","author":"Grabner","year":"1999","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2020.05.005_bib17","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1055\/s-0037-1601868","article-title":"Novel STAC3 mutations in the first non-Amerindian patient with native American myopathy","volume":"48","author":"Grzybowski","year":"2017","journal-title":"Neuropediatrics"},{"key":"10.1016\/j.str.2020.05.005_bib18","doi-asserted-by":"crossref","first-page":"1952","DOI":"10.1038\/ncomms2952","article-title":"Stac3 is a component of the excitation-contraction coupling machinery and mutated in Native American myopathy","volume":"4","author":"Horstick","year":"2013","journal-title":"Nat. Commun."},{"key":"10.1016\/j.str.2020.05.005_bib19","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/B978-0-12-381270-4.00019-6","article-title":"ROSETTA3: an object-oriented software suite for the simulation and design of macromolecules","volume":"487","author":"Leaver-Fay","year":"2011","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.str.2020.05.005_bib20","doi-asserted-by":"crossref","first-page":"E228","DOI":"10.1073\/pnas.1619238114","article-title":"Congenital myopathy results from misregulation of a muscle Ca2+ channel by mutant Stac3","volume":"114","author":"Linsley","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2020.05.005_bib21","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1016\/j.str.2009.08.016","article-title":"Crystal structures of the N-terminal domains of cardiac and skeletal muscle ryanodine receptors: insights into disease mutations","volume":"17","author":"Lobo","year":"2009","journal-title":"Structure"},{"key":"10.1016\/j.str.2020.05.005_bib22","doi-asserted-by":"crossref","first-page":"3696","DOI":"10.1021\/acs.jctc.5b00255","article-title":"ff14SB: improving the accuracy of protein side chain and backbone parameters from ff99SB","volume":"11","author":"Maier","year":"2015","journal-title":"J.\u00a0Chem. Theor. Comput."},{"key":"10.1016\/j.str.2020.05.005_bib23","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser crystallographic software","volume":"40","author":"McCoy","year":"2007","journal-title":"J.\u00a0Appl. Crystallogr."},{"key":"10.1016\/j.str.2020.05.005_bib24","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1107\/S0907444906019949","article-title":"HKL-3000: the integration of data reduction and structure solution--from diffraction images to an initial model in minutes","volume":"62","author":"Minor","year":"2006","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2020.05.005_bib25","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1107\/S0907444911001314","article-title":"REFMAC5 for the refinement of macromolecular crystal structures","volume":"67","author":"Murshudov","year":"2011","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2020.05.005_bib26","doi-asserted-by":"crossref","first-page":"24983","DOI":"10.1074\/jbc.273.39.24983","article-title":"Localization in the II-III loop of the dihydropyridine receptor of a sequence critical for excitation-contraction coupling","volume":"273","author":"Nakai","year":"1998","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2020.05.005_bib27","doi-asserted-by":"crossref","first-page":"11881","DOI":"10.1073\/pnas.1310571110","article-title":"Skeletal muscle-specific T-tubule protein STAC3 mediates voltage-induced Ca2+ release and contractility","volume":"110","author":"Nelson","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2020.05.005_bib28","doi-asserted-by":"crossref","first-page":"e35222","DOI":"10.7554\/eLife.35222","article-title":"Allosteric regulators selectively prevent Ca(2+)-feedback of CaV and NaV channels","volume":"7","author":"Niu","year":"2018","journal-title":"eLife"},{"key":"10.1016\/j.str.2020.05.005_bib47","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","article-title":"Processing of X-ray diffraction data collected in oscillation mode","volume":"276","author":"Otwinowski","year":"1997","journal-title":"Methods Enzymol"},{"key":"10.1016\/j.str.2020.05.005_bib29","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1146\/annurev-genet-120417-031311","article-title":"Calcium channelopathies: structural insights into disorders of the muscle excitation-contraction complex","volume":"52","author":"Pancaroglu","year":"2018","journal-title":"Annu. Rev. Genet."},{"key":"10.1016\/j.str.2020.05.005_bib30","doi-asserted-by":"crossref","first-page":"13822","DOI":"10.1073\/pnas.1716461115","article-title":"De novo reconstitution reveals the proteins required for skeletal muscle voltage-induced Ca(2+) release","volume":"114","author":"Perni","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2020.05.005_bib31","doi-asserted-by":"crossref","first-page":"10986","DOI":"10.1073\/pnas.1612441113","article-title":"Stac3 has a direct role in skeletal muscle-type excitation-contraction coupling that is disrupted by a myopathy-causing mutation","volume":"113","author":"Polster","year":"2016","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2020.05.005_bib32","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1085\/jgp.201711917","article-title":"Stac proteins associate with the critical domain for excitation-contraction coupling in the II-III loop of CaV1.1","volume":"150","author":"Polster","year":"2018","journal-title":"J.\u00a0Gen. Physiol."},{"key":"10.1016\/j.str.2020.05.005_bib33","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1073\/pnas.1423113112","article-title":"Stac adaptor proteins regulate trafficking and function of muscle and neuronal L-type Ca2+ channels","volume":"112","author":"Polster","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2020.05.005_bib34","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1083\/jcb.134.2.375","article-title":"Formation of triads without the dihydropyridine receptor alpha subunits in cell lines from dysgenic skeletal muscle","volume":"134","author":"Powell","year":"1996","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.str.2020.05.005_bib35","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1038\/325717a0","article-title":"Involvement of dihydropyridine receptors in excitation-contraction coupling in skeletal muscle","volume":"325","author":"Rios","year":"1987","journal-title":"Nature"},{"key":"10.1016\/j.str.2020.05.005_bib36","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/0021-9991(77)90098-5","article-title":"Numerical-integration of cartesian equations of motion of a system with constraints - molecular dynamics of n-alkanes","volume":"23","author":"Ryckaert","year":"1977","journal-title":"J.\u00a0Comput. Phys."},{"key":"10.1016\/j.str.2020.05.005_bib37","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1016\/j.neuroscience.2010.02.037","article-title":"Voltage-activated calcium channel expression profiles in mouse brain and cultured hippocampal neurons","volume":"167","author":"Schlick","year":"2010","journal-title":"Neuroscience"},{"key":"10.1016\/j.str.2020.05.005_bib38","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.1002\/ajmg.a.32370","article-title":"Native American myopathy: congenital myopathy with cleft palate, skeletal anomalies, and susceptibility to malignant hyperthermia","volume":"146A","author":"Stamm","year":"2008","journal-title":"Am. J. Med. Genet. A"},{"key":"10.1016\/j.str.2020.05.005_bib39","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1038\/328313a0","article-title":"Primary structure of the receptor for calcium channel blockers from skeletal muscle","volume":"328","author":"Tanabe","year":"1987","journal-title":"Nature"},{"key":"10.1016\/j.str.2020.05.005_bib40","doi-asserted-by":"crossref","first-page":"2763","DOI":"10.1002\/ajmg.a.38375","article-title":"Identification of STAC3 variants in non-Native American families with overlapping features of Carey-Fineman-Ziter syndrome and Moebius syndrome","volume":"173","author":"Telegrafi","year":"2017","journal-title":"Am. J. Med. Genet. A"},{"key":"10.1016\/j.str.2020.05.005_bib41","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/0263-7855(90)80070-V","article-title":"WHAT IF: a molecular modeling and drug design program","volume":"8","author":"Vriend","year":"1990","journal-title":"J.\u00a0Mol. Graph."},{"key":"10.1016\/j.str.2020.05.005_bib42","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.pediatrneurol.2017.01.026","article-title":"Clinicopathologic conference: a newborn with hypotonia, cleft palate, micrognathia, and bilateral club feet","volume":"74","author":"Waldrop","year":"2017","journal-title":"Pediatr. Neurol."},{"key":"10.1016\/j.str.2020.05.005_bib43","doi-asserted-by":"crossref","first-page":"E9520","DOI":"10.1073\/pnas.1708852114","article-title":"Structural insights into binding of STAC proteins to voltage-gated calcium channels","volume":"114","author":"Wong King Yuen","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2020.05.005_bib44","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1016\/j.str.2012.04.015","article-title":"Disease mutations in the ryanodine receptor central region: crystal structures of a phosphorylation hot spot domain","volume":"20","author":"Yuchi","year":"2012","journal-title":"Structure"},{"key":"10.1016\/j.str.2020.05.005_bib45","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1002\/humu.23635","article-title":"STAC3 variants cause a congenital myopathy with distinctive dysmorphic features and malignant hyperthermia susceptibility","volume":"39","author":"Zaharieva","year":"2018","journal-title":"Hum. Mutat."},{"key":"10.1016\/j.str.2020.05.005_bib46","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1016\/j.cell.2019.04.043","article-title":"Molecular basis for ligand modulation of a mammalian voltage-gated Ca(2+) channel","volume":"177","author":"Zhao","year":"2019","journal-title":"Cell"}],"container-title":["Structure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S096921262030174X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S096921262030174X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T08:41:05Z","timestamp":1778575265000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S096921262030174X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":47,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["S096921262030174X"],"URL":"https:\/\/doi.org\/10.1016\/j.str.2020.05.005","relation":{},"ISSN":["0969-2126"],"issn-type":[{"value":"0969-2126","type":"print"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Multiple Sequence Variants in STAC3 Affect Interactions with CaV1.1 and Excitation-Contraction Coupling","name":"articletitle","label":"Article Title"},{"value":"Structure","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.str.2020.05.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}