{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T12:14:47Z","timestamp":1785240887539,"version":"3.55.0"},"reference-count":45,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T00:00:00Z","timestamp":1628035200000},"content-version":"vor","delay-in-days":368,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Structure"],"published-print":{"date-parts":[[2020,8]]},"DOI":"10.1016\/j.str.2020.05.009","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T10:43:27Z","timestamp":1591699407000},"page":"879-887.e3","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"title":["Atomic Structures of Anthrax Prechannel Bound with Full-Length Lethal and Edema Factors"],"prefix":"10.1016","volume":"28","author":[{"given":"Kang","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiheng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan J.","family":"Hardenbrook","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxiang","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan A.","family":"Krantz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z. Hong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.str.2020.05.009_bib1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2020.05.009_bib39","doi-asserted-by":"crossref","first-page":"197","DOI":"10.3390\/toxins8070197","article-title":"Tumor targeting and drug delivery by anthrax toxin","volume":"8","author":"Bachran","year":"2016","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.str.2020.05.009_bib2","first-page":"1","volume":"Vol. 5","author":"Bernard","year":"1995"},{"key":"10.1016\/j.str.2020.05.009_bib40","doi-asserted-by":"crossref","first-page":"2554","DOI":"10.1001\/jama.286.20.2554","article-title":"Death due to bioterrorism-related inhalational anthrax: report of 2 patients","volume":"286","author":"Borio","year":"2001","journal-title":"JAMA"},{"key":"10.1016\/j.str.2020.05.009_bib3","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.ultramic.2013.06.004","article-title":"High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy","volume":"135","author":"Chen","year":"2013","journal-title":"Ultramicroscopy"},{"key":"10.1016\/j.str.2020.05.009_bib4","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1107\/S0907444909042073","article-title":"MolProbity: all-atom structure validation for macromolecular crystallography","volume":"66","author":"Chen","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2020.05.009_bib5","doi-asserted-by":"crossref","first-page":"9062","DOI":"10.1016\/S0021-9258(19)38811-8","article-title":"Substrate requirements of human rhinovirus 3C protease for peptide cleavage in\u00a0vitro","volume":"265","author":"Cordingley","year":"1990","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2020.05.009_bib6","doi-asserted-by":"crossref","first-page":"10","DOI":"10.2174\/1574891X10666150408162354","article-title":"Human anthrax as a re-emerging disease","volume":"10","author":"Doganay","year":"2015","journal-title":"Recent Pat Antiinfect Drug Discov."},{"key":"10.1016\/j.str.2020.05.009_bib7","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1038\/415396a","article-title":"Structural basis for the activation of anthrax adenylyl cyclase exotoxin by calmodulin","volume":"415","author":"Drum","year":"2002","journal-title":"Nature"},{"key":"10.1016\/j.str.2020.05.009_bib8","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2020.05.009_bib9","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1085\/jgp.201611617","article-title":"Structure of anthrax lethal toxin prepore complex suggests a pathway for efficient cell entry","volume":"148","author":"Fabre","year":"2016","journal-title":"J.\u00a0Gen. Physiol."},{"key":"10.1016\/j.str.2020.05.009_bib10","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1038\/nsmb.1923","article-title":"Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers","volume":"17","author":"Feld","year":"2010","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.str.2020.05.009_bib11","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1002\/pro.3235","article-title":"UCSF ChimeraX: meeting modern challenges in visualization and analysis","volume":"27","author":"Goddard","year":"2018","journal-title":"Protein Sci."},{"key":"10.1016\/j.str.2020.05.009_bib12","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1038\/s41586-018-0469-4","article-title":"Malaria parasite translocon structure and mechanism of effector export","volume":"561","author":"Ho","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.str.2020.05.009_bib41","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1089\/hs.2015.0032","article-title":"Antitoxin treatment of inhalation anthrax: a systematic review","volume":"13","author":"Huang","year":"2015","journal-title":"Health Secur."},{"key":"10.1016\/j.str.2020.05.009_bib13","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1038\/nature14247","article-title":"Atomic structure of anthrax protective antigen pore elucidates toxin translocation","volume":"521","author":"Jiang","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.str.2020.05.009_bib14","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.atc.2004.05.009","article-title":"Anthrax","volume":"22","author":"Kalamas","year":"2004","journal-title":"Anesthesiol Clin. North. Am."},{"key":"10.1016\/j.str.2020.05.009_bib15","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.jmb.2009.07.037","article-title":"The protective antigen component of anthrax toxin forms functional octameric complexes","volume":"392","author":"Kintzer","year":"2009","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.str.2020.05.009_bib42","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/j.jmb.2010.04.041","article-title":"Role of the protective antigen octamer in the molecular mechanism of anthrax lethal toxin stabilization in plasma","volume":"399","author":"Kintzer","year":"2010","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.str.2020.05.009_bib16","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1016\/j.jmb.2005.11.030","article-title":"Protein translocation through the anthrax toxin transmembrane pore is driven by a proton gradient","volume":"355","author":"Krantz","year":"2006","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.str.2020.05.009_bib17","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1126\/science.1113380","article-title":"A phenylalanine clamp catalyzes protein translocation through the anthrax toxin pore","volume":"309","author":"Krantz","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.str.2020.05.009_bib18","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/j.jmb.2004.09.067","article-title":"Acid-induced unfolding of the amino-terminal domains of the lethal and edema factors of anthrax toxin","volume":"344","author":"Krantz","year":"2004","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.str.2020.05.009_bib19","doi-asserted-by":"crossref","first-page":"13147","DOI":"10.1073\/pnas.0405405101","article-title":"Structure of heptameric protective antigen bound to an anthrax toxin receptor: a role for receptor in pH-dependent pore formation","volume":"101","author":"Lacy","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.str.2020.05.009_bib43","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1038\/nature17163","article-title":"Crystal structure of a substrate-engaged SecY protein-translocation channel","volume":"531","author":"Li","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.str.2020.05.009_bib44","doi-asserted-by":"crossref","first-page":"2872","DOI":"10.1038\/s41467-019-10918-2","article-title":"Structure of the substrate-engaged SecA-SecY protein translocation machine","volume":"10","author":"Ma","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.str.2020.05.009_bib20","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jsb.2005.07.007","article-title":"Automated electron microscope tomography using robust prediction of specimen movements","volume":"152","author":"Mastronarde","year":"2005","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.str.2020.05.009_bib21","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/S0959-440X(03)00010-1","article-title":"Protein unfolding\u2014an important process in\u00a0vivo?","volume":"13","author":"Matouschek","year":"2003","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.str.2020.05.009_bib22","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/n35101998","article-title":"Crystal structure of the anthrax lethal factor","volume":"414","author":"Pannifer","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.str.2020.05.009_bib23","first-page":"294","article-title":"[Bacillus anthracis: a molecular look at a famous pathogen]","volume":"43","author":"Pavan","year":"2011","journal-title":"Rev. Argent Microbiol."},{"key":"10.1016\/j.str.2020.05.009_bib24","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1038\/385833a0","article-title":"Crystal structure of the anthrax toxin protective antigen","volume":"385","author":"Petosa","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.str.2020.05.009_bib25","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera\u2014a visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.str.2020.05.009_bib26","doi-asserted-by":"crossref","first-page":"9058","DOI":"10.1074\/jbc.M113.452110","article-title":"Engineering anthrax toxin variants that exclusively form octamers and their application to targeting tumors","volume":"288","author":"Phillips","year":"2013","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2020.05.009_bib45","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/S0002-9343(01)01050-6","article-title":"An epidemic of inhalation anthrax, the first in the twentieth century: I. Clinical features. 1960","volume":"112","author":"Plotkin","year":"2002","journal-title":"Am. J. Med."},{"key":"10.1016\/j.str.2020.05.009_bib27","doi-asserted-by":"crossref","first-page":"2303","DOI":"10.3390\/ma12142303","article-title":"Current trends in the biosensors for biological warfare agents assay","volume":"12","author":"Pohanka","year":"2019","journal-title":"Materials (Basel)"},{"key":"10.1016\/j.str.2020.05.009_bib28","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nmeth.4169","article-title":"cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination","volume":"14","author":"Punjani","year":"2017","journal-title":"Nat. Methods"},{"key":"10.1016\/j.str.2020.05.009_bib29","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.jsb.2015.08.008","article-title":"CTFFIND4: fast and accurate defocus estimation from electron micrographs","volume":"192","author":"Rohou","year":"2015","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.str.2020.05.009_bib30","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1038\/nature02763","article-title":"Crystal structure of a complex between anthrax toxin and its host cell receptor","volume":"430","author":"Santelli","year":"2004","journal-title":"Nature"},{"key":"10.1016\/j.str.2020.05.009_bib31","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.jsb.2012.09.006","article-title":"RELION: implementation of a Bayesian approach to cryo-EM structure determination","volume":"180","author":"Scheres","year":"2012","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.str.2020.05.009_bib32","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1038\/sj.emboj.7600574","article-title":"Calcium-independent calmodulin binding and two-metal-ion catalytic mechanism of anthrax edema factor","volume":"24","author":"Shen","year":"2005","journal-title":"EMBO J."},{"key":"10.1016\/j.str.2020.05.009_bib33","doi-asserted-by":"crossref","first-page":"3327","DOI":"10.1093\/bioinformatics\/bti511","article-title":"Resmap: automated representation of macromolecular interfaces as two-dimensional networks","volume":"21","author":"Swint-Kruse","year":"2005","journal-title":"Bioinformatics"},{"key":"10.1016\/j.str.2020.05.009_bib34","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1111\/j.1365-2958.2011.07614.x","article-title":"The unfolding story of anthrax toxin translocation","volume":"80","author":"Thoren","year":"2011","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.str.2020.05.009_bib35","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1126\/science.1113752","article-title":"Protein translocation across biological membranes","volume":"310","author":"Wickner","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.str.2020.05.009_bib36","doi-asserted-by":"crossref","first-page":"43753","DOI":"10.1074\/jbc.M112.419598","article-title":"Electrostatic ratchet in the protective antigen channel promotes anthrax toxin translocation","volume":"287","author":"Wynia-Smith","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2020.05.009_bib37","doi-asserted-by":"crossref","first-page":"3842","DOI":"10.1529\/biophysj.104.050864","article-title":"Protein translocation through anthrax toxin channels formed in planar lipid bilayers","volume":"87","author":"Zhang","year":"2004","journal-title":"Biophys. J."},{"key":"10.1016\/j.str.2020.05.009_bib38","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nmeth.4193","article-title":"MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy","volume":"14","author":"Zheng","year":"2017","journal-title":"Nat. Methods"}],"container-title":["Structure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969212620301787?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969212620301787?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T02:37:40Z","timestamp":1760841460000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0969212620301787"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":45,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["S0969212620301787"],"URL":"https:\/\/doi.org\/10.1016\/j.str.2020.05.009","relation":{},"ISSN":["0969-2126"],"issn-type":[{"value":"0969-2126","type":"print"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Atomic Structures of Anthrax Prechannel Bound with Full-Length Lethal and Edema Factors","name":"articletitle","label":"Article Title"},{"value":"Structure","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.str.2020.05.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}