{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T12:11:51Z","timestamp":1784635911297,"version":"3.55.0"},"reference-count":41,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T00:00:00Z","timestamp":1691539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Structure"],"published-print":{"date-parts":[[2023,11]]},"DOI":"10.1016\/j.str.2023.08.007","type":"journal-article","created":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T11:47:29Z","timestamp":1693482449000},"page":"1386-1393.e3","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["Cryo-EM structure of a human LECT2 amyloid fibril reveals a network of polar ladders at its core"],"prefix":"10.1016","volume":"31","author":[{"given":"Logan S.","family":"Richards","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria D.","family":"Flores","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Zink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie A.","family":"Schibrowsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Sawaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0248-4964","authenticated-orcid":false,"given":"Jose A.","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.str.2023.08.007_bib1","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1016\/j.cell.2012.02.022","article-title":"The Amyloid State of Proteins in Human Diseases","volume":"148","author":"Eisenberg","year":"2012","journal-title":"Cell"},{"key":"10.1016\/j.str.2023.08.007_bib2","doi-asserted-by":"crossref","first-page":"4857","DOI":"10.1016\/j.cell.2021.08.013","article-title":"The expanding amyloid family: Structure, stability, function, and pathogenesis","volume":"184","author":"Sawaya","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.str.2023.08.007_bib3","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/nature23002","article-title":"Cryo-EM structures of tau filaments from Alzheimer\u2019s disease","volume":"547","author":"Fitzpatrick","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.str.2023.08.007_bib4","doi-asserted-by":"crossref","first-page":"5008","DOI":"10.1038\/s41467-019-13038-z","article-title":"Cryo-EM structure of a transthyretin-derived amyloid fibril from a patient with hereditary ATTR amyloidosis","volume":"10","author":"Schmidt","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.str.2023.08.007_bib5","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/0896-6273(91)90052-2","article-title":"The molecular pathology of Alzheimer\u2019s disease","volume":"6","author":"Selkoe","year":"1991","journal-title":"Neuron"},{"key":"10.1016\/j.str.2023.08.007_bib6","doi-asserted-by":"crossref","first-page":"6469","DOI":"10.1073\/pnas.95.11.6469","article-title":"\u03b1-Synuclein in filamentous inclusions of Lewy bodies from Parkinson\u2019s disease and dementia with Lewy bodies","volume":"95","author":"Spillantini","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.str.2023.08.007_bib7","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/joim.13169","article-title":"Systemic amyloidosis from A (AA) to T (ATTR): a review","volume":"289","author":"Muchtar","year":"2021","journal-title":"J.\u00a0Intern. Med."},{"key":"10.1016\/j.str.2023.08.007_bib8","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/s41586-021-03911-7","article-title":"Structure-based classification of tauopathies","volume":"598","author":"Shi","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.str.2023.08.007_bib9","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/ki.2008.152","article-title":"Leukocyte chemotactic factor 2: A novel renal amyloid protein","volume":"74","author":"Benson","year":"2008","journal-title":"Kidney Int."},{"key":"10.1016\/j.str.2023.08.007_bib10","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1038\/modpathol.2016.29","article-title":"Leukocyte chemotactic factor 2 amyloidosis (ALECT2) is a common form of renal amyloidosis among Egyptians","volume":"29","author":"Larsen","year":"2016","journal-title":"Mod. Pathol."},{"key":"10.1016\/j.str.2023.08.007_bib11","first-page":"322","article-title":"The Pathology of Amyloidosis in Classification: A Review","volume":"143","author":"Picken","year":"2020","journal-title":"AHA"},{"key":"10.1016\/j.str.2023.08.007_bib12","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1136\/jim-2021-002149","article-title":"LECT-2 amyloidosis: what do we know?","volume":"70","author":"Mann","year":"2022","journal-title":"J.\u00a0Invest. Med."},{"key":"10.1016\/j.str.2023.08.007_bib13","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0165-2478(96)02572-2","article-title":"Purification and primary amino acid sequence of a novel neutrophil chemotactic factor LECT2","volume":"52","author":"Yamagoe","year":"1996","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.str.2023.08.007_bib14","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1006\/geno.1997.5198","article-title":"Molecular Cloning, Structural Characterization, and Chromosomal Mapping of the Human LECT2 Gene","volume":"48","author":"Yamagoe","year":"1998","journal-title":"Genomics"},{"key":"10.1016\/j.str.2023.08.007_bib15","doi-asserted-by":"crossref","first-page":"22657","DOI":"10.1074\/jbc.271.37.22657","article-title":"A Novel Growth-Promoting Factor Derived from Fetal Bovine Cartilage, Chondromodulin II: PURIFICATION AND AMINO ACID SEQUENCE","volume":"271","author":"Hiraki","year":"1996","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2023.08.007_bib16","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1093\/jn\/nxz333","article-title":"Acute Hyperenergetic, High-Fat Feeding Increases Circulating FGF21, LECT2, and Fetuin-A in Healthy Men","volume":"150","author":"Willis","year":"2020","journal-title":"J.\u00a0Nutr."},{"key":"10.1016\/j.str.2023.08.007_bib17","doi-asserted-by":"crossref","first-page":"17133","DOI":"10.1074\/jbc.M116.720375","article-title":"Crystal Structure of Human Leukocyte Cell-derived Chemotaxin 2 (LECT2) Reveals a Mechanistic Basis of Functional Evolution in a Mammalian Protein with an M23 Metalloendopeptidase Fold","volume":"291","author":"Zheng","year":"2016","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2023.08.007_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2021.100446","article-title":"Loss of bound zinc facilitates amyloid fibril formation of leukocyte-cell-derived chemotaxin 2 (LECT2)","volume":"296","author":"Ha","year":"2021","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2023.08.007_bib19","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1038\/ki.2014.11","article-title":"Clinical, morphologic, and genetic features of renal leukocyte chemotactic factor 2 amyloidosis","volume":"86","author":"Larsen","year":"2014","journal-title":"Kidney Int."},{"key":"10.1016\/j.str.2023.08.007_bib20","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.jsb.2019.06.001","article-title":"Delving into the amyloidogenic core of human leukocyte chemotactic factor 2","volume":"207","author":"Tsiolaki","year":"2019","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.str.2023.08.007_bib21","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/s41586-019-1026-5","article-title":"Novel tau filament fold in chronic traumatic encephalopathy encloses hydrophobic molecules","volume":"568","author":"Falcon","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.str.2023.08.007_bib22","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1107\/S2059798319016577","article-title":"Amyloid structure determination in RELION-3.1","volume":"76","author":"Scheres","year":"2020","journal-title":"Acta Crystallogr. D Struct. Biol."},{"key":"10.1016\/j.str.2023.08.007_bib23","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/319199a0","article-title":"Solvation energy in protein folding and binding","volume":"319","author":"Eisenberg","year":"1986","journal-title":"Nature"},{"key":"10.1016\/j.str.2023.08.007_bib24","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1016\/j.cell.2018.03.032","article-title":"The Structure of the Necrosome RIPK1-RIPK3 Core, a Human Hetero-Amyloid Signaling Complex","volume":"173","author":"Mompe\u00e1n","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.str.2023.08.007_bib25","doi-asserted-by":"crossref","first-page":"3487","DOI":"10.1073\/pnas.0915166107","article-title":"Identifying the amylome, proteins capable of forming amyloid-like fibrils","volume":"107","author":"Goldschmidt","year":"2010","journal-title":"Proc. Natl.\u00a0Acad. Sci. USA"},{"key":"10.1016\/j.str.2023.08.007_bib26","doi-asserted-by":"crossref","first-page":"5355","DOI":"10.1073\/pnas.91.12.5355","article-title":"Glutamine repeats as polar zippers: their possible role in inherited neurodegenerative diseases","volume":"91","author":"Perutz","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.str.2023.08.007_bib27","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1038\/nature03680","article-title":"Structure of the cross-\u03b2 spine of amyloid-like fibrils","volume":"435","author":"Nelson","year":"2005","journal-title":"Nature"},{"key":"10.1016\/j.str.2023.08.007_bib28","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.febslet.2013.01.025","article-title":"Leukocyte cell-derived chemotaxin 2 is a zinc-binding protein","volume":"587","author":"Okumura","year":"2013","journal-title":"FEBS Lett."},{"key":"10.1016\/j.str.2023.08.007_bib29","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/nsmb.1948","article-title":"\u03b22-microglobulin forms three-dimensional domain-swapped amyloid fibrils with disulfide linkages","volume":"18","author":"Liu","year":"2011","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.str.2023.08.007_bib30","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of Coot","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr. D"},{"key":"10.1016\/j.str.2023.08.007_bib31","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1002\/pro.3943","article-title":"UCSF ChimeraX: Structure visualization for researchers, educators, and developers","volume":"30","author":"Pettersen","year":"2021","journal-title":"Protein Sci."},{"key":"10.1016\/j.str.2023.08.007_bib32","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/s42003-019-0437-z","article-title":"SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM","volume":"2","author":"Wagner","year":"2019","journal-title":"Commun. Biol."},{"key":"10.1016\/j.str.2023.08.007_bib33","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.jsb.2012.09.006","article-title":"RELION: Implementation of a Bayesian approach to cryo-EM structure determination","volume":"180","author":"Scheres","year":"2012","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.str.2023.08.007_bib34","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1107\/S2059798319011471","article-title":"Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix","volume":"75","author":"Liebschner","year":"2019","journal-title":"Acta Crystallogr. D"},{"key":"10.1016\/j.str.2023.08.007_bib35","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1093\/bioinformatics\/btq007","article-title":"PyRosetta: a script-based interface for implementing molecular modeling algorithms using Rosetta","volume":"26","author":"Chaudhury","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.str.2023.08.007_bib36","doi-asserted-by":"crossref","first-page":"3551","DOI":"10.1002\/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2","article-title":"Probability-based protein identification by searching sequence databases using mass spectrometry data","volume":"20","author":"Perkins","year":"1999","journal-title":"Electrophoresis"},{"key":"10.1016\/j.str.2023.08.007_bib37","first-page":"613","article-title":"Two particle-picking procedures for filamentous proteins: SPHIRE-crYOLO filament mode and SPHIRE-STRIPER","volume":"76","author":"Wagner","year":"2020","journal-title":"Acta\u00a0Crystallogr. D"},{"key":"10.1016\/j.str.2023.08.007_bib38","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.jsb.2017.02.003","article-title":"Helical reconstruction in RELION","volume":"198","author":"He","year":"2017","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.str.2023.08.007_bib39","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1107\/S205225251801463X","article-title":"A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis","volume":"6","author":"Zivanov","year":"2019","journal-title":"IUCrJ"},{"key":"10.1016\/j.str.2023.08.007_bib40","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1107\/S2052252520000081","article-title":"Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1","volume":"7","author":"Zivanov","year":"2020","journal-title":"IUCrJ"},{"key":"10.1016\/j.str.2023.08.007_bib41","doi-asserted-by":"crossref","first-page":"3031","DOI":"10.1021\/acs.jctc.7b00125","article-title":"The Rosetta All-Atom Energy Function for Macromolecular Modeling and Design","volume":"13","author":"Alford","year":"2017","journal-title":"J.\u00a0Chem. Theor. Comput."}],"container-title":["Structure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S096921262300285X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S096921262300285X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T02:46:36Z","timestamp":1760841996000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S096921262300285X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11]]},"references-count":41,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["S096921262300285X"],"URL":"https:\/\/doi.org\/10.1016\/j.str.2023.08.007","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.02.08.527771","asserted-by":"object"}]},"ISSN":["0969-2126"],"issn-type":[{"value":"0969-2126","type":"print"}],"subject":[],"published":{"date-parts":[[2023,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cryo-EM structure of a human LECT2 amyloid fibril reveals a network of polar ladders at its core","name":"articletitle","label":"Article Title"},{"value":"Structure","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.str.2023.08.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Author(s). Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}