{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:46:52Z","timestamp":1791600412151,"version":"4.3.4"},"reference-count":54,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":370,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82202517"],"award-info":[{"award-number":["82202517"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Structure"],"published-print":{"date-parts":[[2024,6]]},"DOI":"10.1016\/j.str.2024.03.004","type":"journal-article","created":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T10:35:56Z","timestamp":1712226956000},"page":"654-661.e3","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"title":["Structural basis for the inhibition mechanism of the DNA polymerase holoenzyme from mpox virus"],"prefix":"10.1016","volume":"32","author":[{"given":"Yaping","family":"Shen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaning","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7122-6547","authenticated-orcid":false,"given":"Renhong","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.str.2024.03.004_bib1","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/s41579-022-00846-2","article-title":"Long COVID: major findings, mechanisms and recommendations","volume":"21","author":"Davis","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.str.2024.03.004_bib2","doi-asserted-by":"crossref","first-page":"e2392","DOI":"10.1002\/rmv.2392","article-title":"Monkeypox: An international epidemic","volume":"32","author":"Focosi","year":"2022","journal-title":"Rev. Med. Virol."},{"key":"10.1016\/j.str.2024.03.004_bib3","doi-asserted-by":"crossref","first-page":"1076251","DOI":"10.3389\/fcimb.2023.1076251","article-title":"An overview on monkeypox virus: Pathogenesis, transmission, host interaction and therapeutics","volume":"13","author":"Rampogu","year":"2023","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.str.2024.03.004_bib4","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/s41577-022-00775-4","article-title":"Monkeypox: disease epidemiology, host immunity and clinical interventions","volume":"22","author":"Lum","year":"2022","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.str.2024.03.004_bib5","doi-asserted-by":"crossref","first-page":"e0010141","DOI":"10.1371\/journal.pntd.0010141","article-title":"The changing epidemiology of human monkeypox-A potential threat? A systematic review","volume":"16","author":"Bunge","year":"2022","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.str.2024.03.004_bib6","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1097\/00001432-200404000-00003","article-title":"Zoonotic poxvirus infections in humans","volume":"17","author":"Lewis-Jones","year":"2004","journal-title":"Curr. Opin. Infect. Dis."},{"key":"10.1016\/j.str.2024.03.004_bib7","doi-asserted-by":"crossref","first-page":"2127","DOI":"10.1001\/jama.281.22.2127","article-title":"Smallpox as a biological weapon - Medical and public health management","volume":"281","author":"Henderson","year":"1999","journal-title":"JAMA"},{"key":"10.1016\/j.str.2024.03.004_bib8","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/S0140-6736(22)02075-X","article-title":"Monkeypox","volume":"401","author":"Mitj\u00e0","year":"2023","journal-title":"Lancet"},{"key":"10.1016\/j.str.2024.03.004_bib9","doi-asserted-by":"crossref","first-page":"102855","DOI":"10.1016\/j.jaut.2022.102855","article-title":"The 2022 outbreak and the pathobiology of the monkeypox virus","volume":"131","author":"Kumar","year":"2022","journal-title":"J.\u00a0Autoimmun."},{"key":"10.1016\/j.str.2024.03.004_bib10","doi-asserted-by":"crossref","first-page":"E210","DOI":"10.1093\/cid\/ciaa143","article-title":"Clinical Course and Outcome of Human Monkeypox in NigeriaClinical Course and Outcome of Human Monkeypox in Nigeria","volume":"71","author":"Ogoina","year":"2020","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.str.2024.03.004_bib11","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1099\/vir.0.81215-0","article-title":"A tale of two clades: monkeypox viruses","volume":"86","author":"Likos","year":"2005","journal-title":"J.\u00a0Gen. Virol."},{"key":"10.1016\/j.str.2024.03.004_bib12","doi-asserted-by":"crossref","first-page":"6388","DOI":"10.3390\/jcm11216388","article-title":"Genetic Variability of the Monkeypox Virus Clade IIb B.1","volume":"11","author":"Scarpa","year":"2022","journal-title":"J.\u00a0Clin. Med."},{"key":"10.1016\/j.str.2024.03.004_bib13","doi-asserted-by":"crossref","first-page":"e28775","DOI":"10.1002\/jmv.28775","article-title":"Mpox virus Clade IIb detection in the air","volume":"95","author":"Saied","year":"2023","journal-title":"J.\u00a0Med. Virol."},{"key":"10.1016\/j.str.2024.03.004_bib14","doi-asserted-by":"crossref","first-page":"e3001769","DOI":"10.1371\/journal.pbio.3001769","article-title":"Urgent need for a non-discriminatory and non-stigmatizing nomenclature for monkeypox virus","volume":"20","author":"Happi","year":"2022","journal-title":"PLoS Biol."},{"key":"10.1016\/j.str.2024.03.004_bib15","doi-asserted-by":"crossref","first-page":"e0007791","DOI":"10.1371\/journal.pntd.0007791","article-title":"A systematic review of the epidemiology of human monkeypox outbreaks and implications for outbreak strategy","volume":"13","author":"Beer","year":"2019","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.str.2024.03.004_bib16","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1093\/ije\/17.3.643","article-title":"The transmission potential of monkeypox virus in human populations","volume":"17","author":"Fine","year":"1988","journal-title":"Int. J. Epidemiol."},{"key":"10.1016\/j.str.2024.03.004_bib17","first-page":"93","article-title":"The Enzymology of Poxvirus DNA-Replication","volume":"163","author":"Traktman","year":"1990","journal-title":"Curr Top Microbiol."},{"key":"10.1016\/j.str.2024.03.004_bib18","article-title":"Poxvirus DNA Replication","volume":"5","author":"Moss","year":"2013","journal-title":"Csh Perspect Biol."},{"key":"10.1016\/j.str.2024.03.004_bib19","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1146\/annurev-virology-091919-104752","article-title":"The Life Cycle of the Vaccinia Virus Genome","volume":"9","author":"Greseth","year":"2022","journal-title":"Annu. Rev. Virol."},{"key":"10.1016\/j.str.2024.03.004_bib20","doi-asserted-by":"crossref","first-page":"eadg2331","DOI":"10.1126\/sciadv.adg2331","article-title":"Structural basis for the assembly of the DNA polymerase holoenzyme from a monkeypox virus variant","volume":"9","author":"Li","year":"2023","journal-title":"Sci. Adv."},{"key":"10.1016\/j.str.2024.03.004_bib21","doi-asserted-by":"crossref","first-page":"167009","DOI":"10.1016\/j.jmb.2021.167009","article-title":"Solution Structure of the C-terminal Domain of A20, the Missing Brick for the Characterization of the Interface between Vaccinia Virus DNA Polymerase and its Processivity Factor","volume":"433","author":"Bersch","year":"2021","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.str.2024.03.004_bib22","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1128\/JVI.01533-12","article-title":"Low-Resolution Structure of Vaccinia Virus DNA Replication Machinery","volume":"87","author":"S\u00e8le","year":"2013","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.str.2024.03.004_bib23","doi-asserted-by":"crossref","first-page":"12325","DOI":"10.1128\/JVI.01435-10","article-title":"Vaccinia Virus D4 Mutants Defective in Processive DNA Synthesis Retain Binding to A20 and DNA","volume":"84","author":"Druck Shudofsky","year":"2010","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.str.2024.03.004_bib24","doi-asserted-by":"crossref","first-page":"e1003978","DOI":"10.1371\/journal.ppat.1003978","article-title":"Crystal structure of the vaccinia virus DNA polymerase holoenzyme subunit D4 in complex with the A20 N-terminal domain","volume":"10","author":"Contesto-Richefeu","year":"2014","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.str.2024.03.004_bib25","doi-asserted-by":"crossref","first-page":"17923","DOI":"10.1074\/jbc.M115.648352","article-title":"Crystal Structure of the Vaccinia Virus Uracil-DNA Glycosylase in Complex with DNA","volume":"290","author":"Burmeister","year":"2015","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2024.03.004_bib26","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1126\/science.ade6360","article-title":"Structure of monkeypox virus DNA polymerase holoenzyme","volume":"379","author":"Peng","year":"2023","journal-title":"Science"},{"key":"10.1016\/j.str.2024.03.004_bib27","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1038\/s41422-023-00796-1","article-title":"Cryo-EM structures of human monkeypox viral replication complexes with and without DNA duplex","volume":"33","author":"Xu","year":"2023","journal-title":"Cell Res."},{"key":"10.1016\/j.str.2024.03.004_bib28","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0166-3542(02)00196-1","article-title":"Potential antiviral therapeutics for smallpox, monkeypox and other orthopoxvirus infections","volume":"57","author":"Baker","year":"2003","journal-title":"Antiviral Res."},{"key":"10.1016\/j.str.2024.03.004_bib29","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.3390\/v2122681","article-title":"Treatment of Vaccinia and Cowpox Virus Infections in Mice with CMX001 and ST-246","volume":"2","author":"Quenelle","year":"2010","journal-title":"Viruses"},{"key":"10.1016\/j.str.2024.03.004_bib30","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1128\/AAC.46.5.1329-1335.2002","article-title":"Characterization of wild-type and cidofovir-resistant strains of camelpox, cowpox, monkeypox, and vaccinia viruses","volume":"46","author":"Smee","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.str.2024.03.004_bib31","doi-asserted-by":"crossref","first-page":"3153","DOI":"10.1128\/AAC.49.8.3153-3162.2005","article-title":"Mechanism of inhibition of vaccinia virus DNA polymerase by cidofovir diphosphate","volume":"49","author":"Magee","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.str.2024.03.004_bib32","first-page":"197","article-title":"Ara-C: cellular and molecular pharmacology","volume":"72","author":"Grant","year":"1998","journal-title":"Adv. Cancer Res."},{"key":"10.1016\/j.str.2024.03.004_bib33","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/0006-2952(72)90319-X","article-title":"Immunosuppressive, antiviral and antitumor activities of cytarabine derivatives","volume":"21","author":"Gray","year":"1972","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.str.2024.03.004_bib34","doi-asserted-by":"crossref","first-page":"2821","DOI":"10.1099\/0022-1317-83-11-2821","article-title":"Vaccinia virus induces apoptosis of infected macrophages","volume":"83","author":"Humlov\u00e1","year":"2002","journal-title":"J.\u00a0Gen. Virol."},{"key":"10.1016\/j.str.2024.03.004_bib35","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1128\/jvi.65.2.869-879.1991","article-title":"Genetic characterization of the vaccinia virus DNA polymerase: identification of point mutations conferring altered drug sensitivities and reduced fidelity","volume":"65","author":"Taddie","year":"1991","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.str.2024.03.004_bib36","doi-asserted-by":"crossref","first-page":"4323","DOI":"10.1128\/jvi.67.7.4323-4336.1993","article-title":"Genetic characterization of the vaccinia virus DNA polymerase: cytosine arabinoside resistance requires a variable lesion conferring phosphonoacetate resistance in conjunction with an invariant mutation localized to the 3'-5' exonuclease domain","volume":"67","author":"Taddie","year":"1993","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.str.2024.03.004_bib37","doi-asserted-by":"crossref","first-page":"9391","DOI":"10.1128\/JVI.00605-06","article-title":"Cidofovir resistance in vaccinia virus is linked to diminished virulence in mice","volume":"80","author":"Andrei","year":"2006","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.str.2024.03.004_bib38","doi-asserted-by":"crossref","first-page":"4038","DOI":"10.1128\/AAC.00380-06","article-title":"Mutations in the E9L polymerase gene of cidofovir-resistant vaccinia virus strain WR are associated with the drug resistance phenotype","volume":"50","author":"Kornbluth","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.str.2024.03.004_bib39","doi-asserted-by":"crossref","first-page":"12520","DOI":"10.1128\/JVI.01528-08","article-title":"Mechanism of antiviral drug resistance of vaccinia virus: identification of residues in the viral DNA polymerase conferring differential resistance to antipoxvirus drugs","volume":"82","author":"Gammon","year":"2008","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.str.2024.03.004_bib40","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1038\/s41467-017-01542-z","article-title":"The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding","volume":"8","author":"Tarbouriech","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.str.2024.03.004_bib41","first-page":"269","article-title":"Prognostic value of in\u00a0vitro uptake and retention of cytosine arabinoside in acute myelogenous leukemia","volume":"14","author":"Zittoun","year":"1987","journal-title":"Semin. Oncol."},{"key":"10.1016\/j.str.2024.03.004_bib42","first-page":"580","article-title":"Variables predicting response to high dose cytosine arabinoside therapy in patients with refractory acute leukemia","volume":"1","author":"Estey","year":"1987","journal-title":"Leukemia"},{"key":"10.1016\/j.str.2024.03.004_bib43","doi-asserted-by":"crossref","first-page":"25246","DOI":"10.1074\/jbc.M111.248864","article-title":"Phosphonoformic acid inhibits viral replication by trapping the closed form of the DNA polymerase","volume":"286","author":"Zahn","year":"2011","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2024.03.004_bib44","doi-asserted-by":"crossref","first-page":"10006","DOI":"10.1074\/jbc.M116.717405","article-title":"Mechanism of Concerted RNA-DNA Primer Synthesis by the Human Primosome","volume":"291","author":"Baranovskiy","year":"2016","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.str.2024.03.004_bib45","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1038\/s41467-020-14898-6","article-title":"Structure of the processive human Pol \u03b4 holoenzyme","volume":"11","author":"Lancey","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.str.2024.03.004_bib46","doi-asserted-by":"crossref","first-page":"3156","DOI":"10.1038\/s41467-020-16910-5","article-title":"Structure of the polymerase \u03b5 holoenzyme and atomic model of the leading strand replisome","volume":"11","author":"Yuan","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.str.2024.03.004_bib47","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nmeth.4193","article-title":"MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy","volume":"14","author":"Zheng","year":"2017","journal-title":"Nat. Methods"},{"key":"10.1016\/j.str.2024.03.004_bib48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jsb.2015.11.003","article-title":"Gctf: Real-time CTF determination and correction","volume":"193","author":"Zhang","year":"2016","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.str.2024.03.004_bib49","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nmeth.4169","article-title":"cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination","volume":"14","author":"Punjani","year":"2017","journal-title":"Nat. Methods"},{"key":"10.1016\/j.str.2024.03.004_bib50","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of Coot. Acta crystallographica","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2024.03.004_bib51","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera--a visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.str.2024.03.004_bib52","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2024.03.004_bib53","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.str.2008.03.005","article-title":"Flexible Fitting of Atomic Structures into Electron Microscopy Maps Using Molecular Dynamics","volume":"16","author":"Trabuco","year":"2008","journal-title":"Structure"},{"key":"10.1016\/j.str.2024.03.004_bib54","doi-asserted-by":"crossref","first-page":"e06980","DOI":"10.7554\/eLife.06980","article-title":"Measuring the optimal exposure for single particle cryo-EM using a 2.6\u00a0\u00c5 reconstruction of rotavirus VP6","volume":"4","author":"Grant","year":"2015","journal-title":"Elife"}],"container-title":["Structure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969212624000868?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0969212624000868?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T13:01:19Z","timestamp":1762347679000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0969212624000868"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":54,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["S0969212624000868"],"URL":"https:\/\/doi.org\/10.1016\/j.str.2024.03.004","relation":{},"ISSN":["0969-2126"],"issn-type":[{"value":"0969-2126","type":"print"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural basis for the inhibition mechanism of the DNA polymerase holoenzyme from mpox virus","name":"articletitle","label":"Article Title"},{"value":"Structure","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.str.2024.03.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}