{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:41:45Z","timestamp":1790811705097,"version":"4.1.0"},"reference-count":34,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["S10OD021832"],"award-info":[{"award-number":["S10OD021832"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P30 GM124169"],"award-info":[{"award-number":["P30 GM124169"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006132","name":"Office of Science","doi-asserted-by":"publisher","award":["DE-AC02-05CH11231"],"award-info":[{"award-number":["DE-AC02-05CH11231"]}],"id":[{"id":"10.13039\/100006132","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Structure"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.str.2025.11.002","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T16:09:20Z","timestamp":1764259760000},"page":"264-272.e3","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["The therapeutic antibody sacituzumab induces trophoblast cell-surface antigen-2 conformational rearrangement"],"prefix":"10.1016","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6697-0611","authenticated-orcid":false,"given":"Ryan","family":"Ferrao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Chien","family":"Chou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Nieto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek","family":"Langeslay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moon","family":"Chatterjee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debi","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdeleine","family":"Hung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Scott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Nagel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weimei","family":"Xing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Letarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Ambrogelly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric B.","family":"Lansdon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.str.2025.11.002_bib1","doi-asserted-by":"crossref","first-page":"5147","DOI":"10.1073\/pnas.78.8.5147","article-title":"Human trophoblast cell-surface antigens defined by monoclonal antibodies","volume":"78","author":"Lipinski","year":"1981","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.str.2025.11.002_bib2","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1002\/ijc.2910600113","article-title":"Transformation of human keratinocytes is characterized by quantitative and qualitative alterations of the T-16 antigen (Trop-2, MOv-16)","volume":"60","author":"Schon","year":"1995","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.str.2025.11.002_bib3","first-page":"5325","article-title":"Alterations in gene expression profiles during prostate cancer progression: functional correlations to tumorigenicity and down-regulation of selenoprotein-P in mouse and human tumors","volume":"62","author":"Calvo","year":"2002","journal-title":"Cancer Res."},{"key":"10.1016\/j.str.2025.11.002_bib4","first-page":"1507","article-title":"Retroposition in a family of carcinoma-associated antigen genes","volume":"13","author":"Linnenbach","year":"1993","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.str.2025.11.002_bib5","doi-asserted-by":"crossref","first-page":"857","DOI":"10.2174\/1568009618666180102102311","article-title":"Antibody Based EpCAM Targeted Therapy of Cancer, Review and Update","volume":"18","author":"Eyvazi","year":"2018","journal-title":"Curr. Cancer Drug Targets"},{"key":"10.1016\/j.str.2025.11.002_bib6","doi-asserted-by":"crossref","first-page":"2271","DOI":"10.1101\/gad.196451.112","article-title":"Regulated proteolysis of Trop2 drives epithelial hyperplasia and stem cell self-renewal via beta-catenin signaling","volume":"26","author":"Stoyanova","year":"2012","journal-title":"Genes Dev."},{"key":"10.1016\/j.str.2025.11.002_bib7","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.neo.2021.03.006","article-title":"Trop-2 cleavage by ADAM10 is an activator switch for cancer growth and metastasis","volume":"23","author":"Trerotola","year":"2021","journal-title":"Neoplasia"},{"key":"10.1016\/j.str.2025.11.002_bib8","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1002\/cam4.1934","article-title":"The role and molecular mechanism of Trop2 induced epithelial-mesenchymal transition through mediated beta-catenin in gastric cancer","volume":"8","author":"Zhao","year":"2019","journal-title":"Cancer Med."},{"key":"10.1016\/j.str.2025.11.002_bib9","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.11484","article-title":"Destabilization of EpCAM dimer is associated with increased susceptibility towards cleavage by TACE","volume":"9","author":"Zagar","year":"2021","journal-title":"PeerJ"},{"key":"10.1016\/j.str.2025.11.002_bib10","doi-asserted-by":"crossref","first-page":"24574","DOI":"10.1074\/jbc.M115.662700","article-title":"Cleavage and cell adhesion properties of human epithelial cell adhesion molecule (HEPCAM)","volume":"290","author":"Tsaktanis","year":"2015","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.str.2025.11.002_bib11","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.pep.2013.07.006","article-title":"Biochemical and preliminary X-ray characterization of the tumor-associated calcium signal transducer 2 (Trop2) ectodomain","volume":"91","author":"Vidmar","year":"2013","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.str.2025.11.002_bib12","doi-asserted-by":"crossref","first-page":"10640","DOI":"10.3390\/ijms221910640","article-title":"Trop2 Forms a Stable Dimer with Significant Structural Differences within the Membrane-Distal Region as Compared to EpCAM","volume":"22","author":"Pavsic","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.str.2025.11.002_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2021.103190","article-title":"Structural insights into the cis and trans assembly of human trophoblast cell surface antigen 2","volume":"24","author":"Sun","year":"2021","journal-title":"iScience"},{"key":"10.1016\/j.str.2025.11.002_bib14","doi-asserted-by":"crossref","first-page":"3156","DOI":"10.1002\/1873-3468.13899","article-title":"Proteolytic cleavage of Trop2 at Arg87 is mediated by matriptase and regulated by Val194","volume":"594","author":"Kamble","year":"2020","journal-title":"FEBS Lett."},{"key":"10.1016\/j.str.2025.11.002_bib15","first-page":"1330","article-title":"Murine monoclonal antibodies raised against human non-small cell carcinoma of the lung: specificity and tumor targeting","volume":"50","author":"Stein","year":"1990","journal-title":"Cancer Res."},{"key":"10.1016\/j.str.2025.11.002_bib16","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1002\/ijc.2910550611","article-title":"Specificity and properties of MAb RS7-3G11 and the antigen defined by this pancarcinoma monoclonal antibody","volume":"55","author":"Stein","year":"1993","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.str.2025.11.002_bib17","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/ijc.2910570721","article-title":"Characterization of cluster 13: the epithelial\/carcinoma antigen recognized by MAb RS7","volume":"8","author":"Stein","year":"1994","journal-title":"Int. J. Cancer Suppl."},{"key":"10.1016\/j.str.2025.11.002_bib18","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1002\/1097-0142(19940201)73:3+<816::AID-CNCR2820731311>3.0.CO;2-T","article-title":"Comparative biodistribution and radioimmunotherapy of monoclonal antibody RS7 and its F(ab')2 in nude mice bearing human tumor xenografts","volume":"73","author":"Stein","year":"1994","journal-title":"Cancer"},{"key":"10.1016\/j.str.2025.11.002_bib19","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1023\/B:BREA.0000018417.02580.ef","article-title":"Preclinical therapy of breast cancer with a radioiodinated humanized anti-EGP-1 monoclonal antibody: advantage of a residualizing iodine radiolabel","volume":"84","author":"Govindan","year":"2004","journal-title":"Breast Cancer Res. Treat."},{"key":"10.1016\/j.str.2025.11.002_bib20","doi-asserted-by":"crossref","first-page":"3157","DOI":"10.1158\/1078-0432.CCR-10-2939","article-title":"Humanized anti-Trop-2 IgG-SN-38 conjugate for effective treatment of diverse epithelial cancers: preclinical studies in human cancer xenograft models and monkeys","volume":"17","author":"Cardillo","year":"2011","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.str.2025.11.002_bib21","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1038\/bjc.2017.367","article-title":"Antibody drug conjugates and bystander killing: is antigen-dependent internalisation required?","volume":"117","author":"Staudacher","year":"2017","journal-title":"Br. J. Cancer"},{"key":"10.1016\/j.str.2025.11.002_bib22","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1158\/1535-7163.MCT-22-0375","article-title":"Antibody-Drug Conjugate Sacituzumab Govitecan Drives Efficient Tissue Penetration and Rapid Intracellular Drug Release","volume":"22","author":"Kopp","year":"2023","journal-title":"Mol. Cancer Ther."},{"key":"10.1016\/j.str.2025.11.002_bib23","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1056\/NEJMoa2028485","article-title":"Sacituzumab Govitecan in Metastatic Triple-Negative Breast Cancer","volume":"384","author":"Bardia","year":"2021","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.str.2025.11.002_bib24","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1056\/NEJMoa1814213","article-title":"Sacituzumab Govitecan-hziy in Refractory Metastatic Triple-Negative Breast Cancer","volume":"380","author":"Bardia","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.str.2025.11.002_bib25","doi-asserted-by":"crossref","first-page":"2474","DOI":"10.1200\/JCO.20.03489","article-title":"TROPHY-U-01: A Phase II Open-Label Study of Sacituzumab Govitecan in Patients With Metastatic Urothelial Carcinoma Progressing After Platinum-Based Chemotherapy and Checkpoint Inhibitors","volume":"39","author":"Tagawa","year":"2021","journal-title":"J. Clin. Oncol."},{"key":"10.1016\/j.str.2025.11.002_bib26","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/d41573-024-00064-w","article-title":"The antibody-drug conjugate landscape","volume":"23","author":"Flynn","year":"2024","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.str.2025.11.002_bib27","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1006\/jmbi.1993.1648","article-title":"Shape complementarity at protein\/protein interfaces","volume":"234","author":"Lawrence","year":"1993","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.str.2025.11.002_bib28","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1021\/acsmedchemlett.8b00147","article-title":"Reviving B-Factors: Retrospective Normalized B-Factor Analysis of c-ros Oncogene 1 Receptor Tyrosine Kinase and Anaplastic Lymphoma Kinase L1196M with Crizotinib and Lorlatinib","volume":"9","author":"Johnson","year":"2018","journal-title":"ACS Med. Chem. Lett."},{"key":"10.1016\/j.str.2025.11.002_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2019.120312","article-title":"Variation of Trop2 on non-small-cell lung cancer and normal cell membranes revealed by super-resolution fluorescence imaging","volume":"207","author":"Fu","year":"2020","journal-title":"Talanta"},{"key":"10.1016\/j.str.2025.11.002_bib30","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","article-title":"Xds","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2025.11.002_bib31","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser crystallographic software","volume":"40","author":"McCoy","year":"2007","journal-title":"J. Appl. Crystallogr."},{"key":"10.1016\/j.str.2025.11.002_bib32","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1107\/S2059798319011471","article-title":"Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix","volume":"75","author":"Liebschner","year":"2019","journal-title":"Acta Crystallogr. D Struct. Biol."},{"key":"10.1016\/j.str.2025.11.002_bib33","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of Coot","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.str.2025.11.002_bib34","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.jsb.2005.03.010","article-title":"Automated molecular microscopy: the new Leginon system","volume":"151","author":"Suloway","year":"2005","journal-title":"J. Struct. Biol."}],"container-title":["Structure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S096921262500437X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S096921262500437X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T14:59:20Z","timestamp":1773932360000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S096921262500437X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["S096921262500437X"],"URL":"https:\/\/doi.org\/10.1016\/j.str.2025.11.002","relation":{},"ISSN":["0969-2126"],"issn-type":[{"value":"0969-2126","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The therapeutic antibody sacituzumab induces trophoblast cell-surface antigen-2 conformational rearrangement","name":"articletitle","label":"Article Title"},{"value":"Structure","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.str.2025.11.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}