{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:52:22Z","timestamp":1760889142096,"version":"build-2065373602"},"reference-count":35,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["13022-717"],"award-info":[{"award-number":["13022-717"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB 1127"],"award-info":[{"award-number":["SFB 1127"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Systematic and Applied Microbiology"],"published-print":{"date-parts":[[2016,3]]},"DOI":"10.1016\/j.syapm.2016.01.002","type":"journal-article","created":{"date-parts":[[2016,2,11]],"date-time":"2016-02-11T13:30:36Z","timestamp":1455197436000},"page":"132-140","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"title":["Fast label-free detection of Legionella spp. in biofilms by applying immunomagnetic beads and Raman spectroscopy"],"prefix":"10.1016","volume":"39","author":[{"given":"Dragana","family":"Kusi\u0107","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"R\u00f6sch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Popp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.syapm.2016.01.002_bib0180","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.mimet.2006.07.002","article-title":"Development and evaluation of a Taqman duplex real-time PCR quantification method for reliable enumeration of Legionella pneumophila in water samples","volume":"68","author":"Behets","year":"2007","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.syapm.2016.01.002_bib0185","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1590\/S1517-83822007000400029","article-title":"Detection of Legionella pneumophila in water and biofilm samples by culture and molecular methods from man-made systems in S\u00e3o Paulo-Brazil","volume":"38","author":"Carvalho","year":"2007","journal-title":"Braz. J. Microbiol."},{"key":"10.1016\/j.syapm.2016.01.002_bib0190","doi-asserted-by":"crossref","first-page":"2929","DOI":"10.1007\/s00216-010-3895-1","article-title":"The influence of intracellular storage material on bacterial identification by means of Raman spectroscopy","volume":"397","author":"Ciobot\u0103","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.syapm.2016.01.002_bib0195","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1023\/A:1022627411411","article-title":"Support-vector networks","volume":"20","author":"Cortes","year":"1995","journal-title":"Mach. Learn."},{"key":"10.1016\/j.syapm.2016.01.002_bib0200","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1186\/s12866-015-0423-7","article-title":"Comparing methods of determining Legionella spp. in complex water matrices","volume":"15","author":"D\u00edaz-Flores","year":"2015","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.syapm.2016.01.002_bib0205","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1128\/CMR.15.3.506-526.2002","article-title":"Legionella and Legionnaires\u2019 disease: 25 years of investigation","volume":"15","author":"Fields","year":"2002","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.syapm.2016.01.002_bib0210","first-page":"437","article-title":"Rapid identification of Legionella pathogenicity by surface-enhanced Raman spectroscopy","volume":"28","author":"Jing","year":"2015","journal-title":"Biomed. Environ. Sci."},{"year":"2013","series-title":"kernlab: Kernel-based Machine Learning Lab","author":"Karatzoglou","key":"10.1016\/j.syapm.2016.01.002_bib0215"},{"key":"10.1016\/j.syapm.2016.01.002_bib0220","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s13758-012-0067-1","article-title":"Polythiophene synthesis coupled to quartz crystal microbalance and Raman spectroscopy for detecting bacteria","volume":"7","author":"Kengne-Momo","year":"2012","journal-title":"Biointerphase"},{"key":"10.1016\/j.syapm.2016.01.002_bib0225","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1111\/j.1751-7915.2012.00366.x","article-title":"Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry","volume":"5","author":"Keserue","year":"2012","journal-title":"Microb. Biotechnol."},{"key":"10.1016\/j.syapm.2016.01.002_bib0230","doi-asserted-by":"crossref","first-page":"9610","DOI":"10.1021\/ac401806f","article-title":"Culture independent Raman spectroscopic identification of urinary tract infection pathogens: a proof of principle study","volume":"85","author":"Klo\u00df","year":"2013","journal-title":"Anal. Chem."},{"key":"10.1016\/j.syapm.2016.01.002_bib0235","doi-asserted-by":"crossref","first-page":"8333","DOI":"10.1007\/s00216-015-8743-x","article-title":"Destruction-free procedure for the isolation of bacteria from sputum samples for Raman spectroscopic analysis","volume":"407","author":"Klo\u00df","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.syapm.2016.01.002_bib0240","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1021\/ac503373r","article-title":"Toward culture-free Raman spectroscopic identification of pathogens in ascitic fluid","volume":"87","author":"Klo\u00df","year":"2014","journal-title":"Anal. Chem."},{"key":"10.1016\/j.syapm.2016.01.002_bib0245","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.1007\/s00216-011-5398-0","article-title":"A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria","volume":"402","author":"Knauer","year":"2012","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.syapm.2016.01.002_bib0250","doi-asserted-by":"crossref","first-page":"4584","DOI":"10.1039\/C5AN00438A","article-title":"Characterization of carotenoids in soil bacteria and investigation of their photodegradation by UVA radiation via resonance Raman spectroscopy","volume":"140","author":"Kumar","year":"2015","journal-title":"Analyst"},{"key":"10.1016\/j.syapm.2016.01.002_bib0255","doi-asserted-by":"crossref","first-page":"6803","DOI":"10.1007\/s00216-015-8851-7","article-title":"Raman spectroscopic differentiation of planktonic bacteria and biofilms","volume":"407","author":"Kusi\u0107","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.syapm.2016.01.002_bib0260","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.watres.2013.09.030","article-title":"Identification of water pathogens by Raman microspectroscopy","volume":"48","author":"Kusi\u0107","year":"2014","journal-title":"Water Res."},{"key":"10.1016\/j.syapm.2016.01.002_bib0265","doi-asserted-by":"crossref","first-page":"2885","DOI":"10.1128\/AEM.72.4.2885-2895.2006","article-title":"Planktonic replication is essential for biofilm formation by Legionella pneumophila in a complex medium under static and dynamic flow conditions","volume":"72","author":"Mampel","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.syapm.2016.01.002_bib0270","doi-asserted-by":"crossref","first-page":"4997","DOI":"10.1039\/c1an15761b","article-title":"Assessment of two isolation techniques for bacteria in milk towards their compatibility with Raman spectroscopy","volume":"136","author":"Meisel","year":"2011","journal-title":"Analyst"},{"key":"10.1016\/j.syapm.2016.01.002_bib0275","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.fm.2013.08.007","article-title":"Identification of meat-associated pathogens via Raman microspectroscopy","volume":"38","author":"Meisel","year":"2014","journal-title":"Food Microbiol."},{"key":"10.1016\/j.syapm.2016.01.002_bib0280","doi-asserted-by":"crossref","first-page":"3121","DOI":"10.1099\/00221287-147-11-3121","article-title":"Role of biofilms in the survival of Legionella pneumophila in a model potable-water system","volume":"147","author":"Murga","year":"2001","journal-title":"Microbiology"},{"key":"10.1016\/j.syapm.2016.01.002_bib0285","doi-asserted-by":"crossref","first-page":"3600","DOI":"10.1002\/cphc.201300543","article-title":"Isolation and enrichment of pathogens with a surface-modified aluminium chip for Raman spectroscopic applications","volume":"14","author":"Pahlow","year":"2013","journal-title":"ChemPhysChem"},{"key":"10.1016\/j.syapm.2016.01.002_bib0290","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.addr.2015.04.006","article-title":"Isolation and identification of bacteria by means of Raman spectroscopy","volume":"89","author":"Pahlow","year":"2015","journal-title":"Adv. Drug. Deliv. Rev."},{"key":"10.1016\/j.syapm.2016.01.002_bib0295","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/347301a0","article-title":"Studying single living cells and chromosomes by confocal Raman microspectroscopy","volume":"347","author":"Puppels","year":"1990","journal-title":"Nature"},{"key":"10.1016\/j.syapm.2016.01.002_bib0300","doi-asserted-by":"crossref","first-page":"99","DOI":"10.2166\/wst.2001.0719","article-title":"Occurrence of Legionella in groundwater: an ecological study","volume":"43","author":"Riffard","year":"2001","journal-title":"Water Sci. Technol."},{"key":"10.1016\/j.syapm.2016.01.002_bib0305","doi-asserted-by":"crossref","first-page":"2326","DOI":"10.1128\/aem.58.7.2326-2330.1992","article-title":"Immunogold and fluorescein immunolabelling of Legionella pneumophila within an aquatic biofilm visualized by using episcopic differential interference contrast microscopy","volume":"58","author":"Rogers","year":"1992","journal-title":"App. Environ. Microbiol."},{"key":"10.1016\/j.syapm.2016.01.002_bib0310","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1002\/jbio.201200131","article-title":"Discrimination of single-porin Escherichia (E.) coli mutants by ATR and transmission mode FTIR spectroscopy","volume":"7","author":"Saraiva","year":"2014","journal-title":"J. Biophoton."},{"key":"10.1016\/j.syapm.2016.01.002_bib0315","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.syapm.2014.05.007","article-title":"Identification of water-conditioned Pseudomonas aeruginosa by Raman microspectroscopy on a single cell level","volume":"37","author":"Silge","year":"2014","journal-title":"Syst. Appl. Microbiol."},{"key":"10.1016\/j.syapm.2016.01.002_bib0320","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1002\/jbio.201200075","article-title":"Identification of carbapenem-resistant Acinetobacter baumannii clones using infrared spectroscopy","volume":"7","author":"Sousa","year":"2014","journal-title":"J. Biophoton."},{"key":"10.1016\/j.syapm.2016.01.002_bib0325","doi-asserted-by":"crossref","first-page":"5339","DOI":"10.1002\/anie.201201266","article-title":"Raman spectroscopic detection of anthrax endospores in powder samples","volume":"51","author":"St\u00f6ckel","year":"2012","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.syapm.2016.01.002_bib0330","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1006\/immu.1993.1051","article-title":"Crystallization of antibodies and antibody\u2013antigen complexes","volume":"3","author":"Stura","year":"1993","journal-title":"Immunomethods"},{"key":"10.1016\/j.syapm.2016.01.002_bib0335","doi-asserted-by":"crossref","first-page":"3167","DOI":"10.1007\/s11051-010-0213-y","article-title":"Fabrication of magnetic gold nanorod particles for immunomagnetic separation and SERS application","volume":"13","author":"Tamer","year":"2011","journal-title":"J. Nanopart. Res."},{"key":"10.1016\/j.syapm.2016.01.002_bib0340","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1146\/annurev.micro.091208.073233","article-title":"Single-cell ecophysiology of microbes as revealed by Raman microspectroscopy or secondary ion mass spectrometry imaging","volume":"63","author":"Wagner","year":"2009","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.syapm.2016.01.002_bib0345","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1007\/s00216-010-4453-6","article-title":"Separation and detection of multiple pathogens in a food matrix by magnetic SERS nanoprobes","volume":"399","author":"Wang","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.syapm.2016.01.002_bib0350","doi-asserted-by":"crossref","first-page":"3433","DOI":"10.1128\/AEM.71.7.3433-3441.2005","article-title":"Quantitative detection of Legionella pneumophila in water samples by immunomagnetic purification and real-time PCR amplification of the dotA gene","volume":"71","author":"Y\u00e1\u00f1ez","year":"2005","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Systematic and Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0723202016000163?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0723202016000163?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T08:11:08Z","timestamp":1759997468000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0723202016000163"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3]]},"references-count":35,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["S0723202016000163"],"URL":"https:\/\/doi.org\/10.1016\/j.syapm.2016.01.002","relation":{},"ISSN":["0723-2020"],"issn-type":[{"type":"print","value":"0723-2020"}],"subject":[],"published":{"date-parts":[[2016,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Fast label-free detection of Legionella spp. in biofilms by applying immunomagnetic beads and Raman spectroscopy","name":"articletitle","label":"Article Title"},{"value":"Systematic and Applied Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.syapm.2016.01.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}]}}