{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T02:44:43Z","timestamp":1770777883005,"version":"3.50.0"},"reference-count":41,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T00:00:00Z","timestamp":1684713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070083"],"award-info":[{"award-number":["32070083"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32222004"],"award-info":[{"award-number":["32222004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004739","name":"Youth Innovation Promotion Association of the Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["2021177"],"award-info":[{"award-number":["2021177"]}],"id":[{"id":"10.13039\/501100004739","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0901500"],"award-info":[{"award-number":["2018YFA0901500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Synthetic and Systems Biotechnology"],"published-print":{"date-parts":[[2023,9]]},"DOI":"10.1016\/j.synbio.2023.05.004","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T12:18:26Z","timestamp":1686226706000},"page":"386-395","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde"],"prefix":"10.1016","volume":"8","author":[{"given":"Jin","family":"Qian","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liwen","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinxing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinhui","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guimin","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Pu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjuan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jibin","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Depei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0957-9245","authenticated-orcid":false,"given":"Yu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"6320","key":"10.1016\/j.synbio.2023.05.004_bib1","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1126\/science.aag0804","article-title":"Industrial biomanufacturing: the future of chemical production","volume":"355","author":"Clomburg","year":"2017","journal-title":"Science"},{"issue":"6","key":"10.1016\/j.synbio.2023.05.004_bib2","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1016\/j.tibtech.2019.12.013","article-title":"Synthetic methylotrophy: a practical solution for methanol-based biomanufacturing","volume":"38","author":"Wang","year":"2020","journal-title":"Trends Biotechnol"},{"issue":"6562","key":"10.1016\/j.synbio.2023.05.004_bib3","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1126\/science.abh4049","article-title":"Cell-free chemoenzymatic starch synthesis from carbon dioxide","volume":"373","author":"Cai","year":"2021","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.synbio.2023.05.004_bib4","doi-asserted-by":"crossref","first-page":"10006","DOI":"10.1016\/j.engmic.2021.100006","article-title":"A chemoenzymatic cascade with the potential to feed the world and allow humans to live in space","volume":"2","author":"Wu","year":"2022","journal-title":"Eng Microbiol"},{"key":"10.1016\/j.synbio.2023.05.004_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2021.787791","article-title":"Methanol dehydrogenases as a key biocatalysts for synthetic methylotrophy","volume":"9","author":"Le","year":"2021","journal-title":"Front Bioeng Biotechnol"},{"key":"10.1016\/j.synbio.2023.05.004_bib6","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.copbio.2015.01.007","article-title":"Synthetic methylotrophy: engineering the production of biofuels and chemicals based on the biology of aerobic methanol utilization","volume":"33","author":"Whitaker","year":"2015","journal-title":"Curr Opin Biotechnol"},{"issue":"4","key":"10.1016\/j.synbio.2023.05.004_bib7","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.cell.2020.07.010","article-title":"Converting Escherichia coli to a synthetic methylotroph growing solely on methanol","volume":"182","author":"Chen","year":"2020","journal-title":"Cell"},{"issue":"11","key":"10.1016\/j.synbio.2023.05.004_bib8","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1021\/acssynbio.9b00102","article-title":"An aldolase-catalyzed new metabolic pathway for the assimilation of formaldehyde and methanol to synthesize 2-keto-4-hydroxybutyrate and 1,3-propanediol in Escherichia coli","volume":"8","author":"Chuang","year":"2019","journal-title":"ACS Synth Biol"},{"issue":"6","key":"10.1016\/j.synbio.2023.05.004_bib9","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.1021\/acssynbio.8b00093","article-title":"Ribulose monophosphate shunt provides nearly all biomass and energy required for growth of E. coli","volume":"7","author":"He","year":"2018","journal-title":"ACS Synth Biol"},{"key":"10.1016\/j.synbio.2023.05.004_bib10","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.ymben.2018.07.011","article-title":"Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production","volume":"49","author":"Tuyishime","year":"2018","journal-title":"Metab Eng"},{"issue":"1","key":"10.1016\/j.synbio.2023.05.004_bib11","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1038\/s42003-020-0954-9","article-title":"Adaptive laboratory evolution enhances methanol tolerance and conversion in engineered Corynebacterium glutamicum","volume":"3","author":"Wang","year":"2020","journal-title":"Commun Biol"},{"issue":"10","key":"10.1016\/j.synbio.2023.05.004_bib12","doi-asserted-by":"crossref","first-page":"3617","DOI":"10.3390\/ijms21103617","article-title":"Methanol-essential growth of Corynebacterium glutamicum: adaptive laboratory evolution overcomes limitation due to methanethiol assimilation pathway","volume":"21","author":"Hennig","year":"2020","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.synbio.2023.05.004_bib13","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.jbiotec.2021.12.005","article-title":"Constructing a methanol-dependent Bacillus subtilis by engineering the methanol metabolism","volume":"343","author":"Bo","year":"2022","journal-title":"J Biotechnol"},{"key":"10.1016\/j.synbio.2023.05.004_bib14","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1016\/j.biortech.2017.05.100","article-title":"Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae","volume":"245","author":"Dai","year":"2017","journal-title":"Bioresour Technol"},{"issue":"16","key":"10.1016\/j.synbio.2023.05.004_bib15","doi-asserted-by":"crossref","first-page":"5346","DOI":"10.1128\/jb.174.16.5346-5353.1992","article-title":"Cloning, expression, and sequence-analysis of the Bacillus methanolicus C1 methanol dehydrogenase gene","volume":"174","author":"Devries","year":"1992","journal-title":"J Bacteriol"},{"issue":"3","key":"10.1016\/j.synbio.2023.05.004_bib16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0059188","article-title":"Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties","volume":"8","author":"Krog","year":"2013","journal-title":"PLoS One"},{"issue":"11","key":"10.1016\/j.synbio.2023.05.004_bib17","doi-asserted-by":"crossref","first-page":"4969","DOI":"10.1007\/s00253-016-7320-3","article-title":"Characterization and evolution of an activator-independent methanol dehydrogenase from Cupriavidus necator N-1","volume":"100","author":"Wu","year":"2016","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.synbio.2023.05.004_bib18","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1042\/bj2520661","article-title":"A new alcohol dehydrogenase, reactive towards methanol, from Bacillus stearothermophilus","volume":"252","author":"Marian","year":"1988","journal-title":"Biochem J"},{"key":"10.1016\/j.synbio.2023.05.004_bib19","doi-asserted-by":"crossref","first-page":"67","DOI":"10.3389\/fbioe.2020.00067","article-title":"Discovery and biochemical characterization of a methanol dehydrogenase from Lysinibacillus xylanilyticus","volume":"8","author":"Lee","year":"2020","journal-title":"Front Bioeng Biotechnol"},{"issue":"2","key":"10.1016\/j.synbio.2023.05.004_bib20","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1128\/jb.99.2.414-417.1969","article-title":"Prototrophic thermophilic Bacillus: isolation, properties, and kinetics of growth","volume":"99","author":"Israel","year":"1969","journal-title":"J Bacteriol"},{"issue":"23","key":"10.1016\/j.synbio.2023.05.004_bib21","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1002\/cbic.201800424","article-title":"Engineering artificial fusion proteins for enhanced methanol bioconversion","volume":"19","author":"Fan","year":"2018","journal-title":"Chembiochem"},{"key":"10.1016\/j.synbio.2023.05.004_bib22","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.ymben.2016.10.015","article-title":"Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli","volume":"39","author":"Whitaker","year":"2017","journal-title":"Metab Eng"},{"issue":"8","key":"10.1016\/j.synbio.2023.05.004_bib23","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1021\/acssynbio.7b00114","article-title":"Sort-Seq approach to engineering a formaldehyde-inducible promoter for dynamically regulated Escherichia coli growth on methanol","volume":"6","author":"Rohlhill","year":"2017","journal-title":"ACS Synth Biol"},{"issue":"1","key":"10.1016\/j.synbio.2023.05.004_bib24","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1002\/bit.26455","article-title":"Development of a formaldehyde biosensor with application to synthetic methylotrophy","volume":"115","author":"Woolston","year":"2018","journal-title":"Biotechnol Bioeng"},{"issue":"9","key":"10.1016\/j.synbio.2023.05.004_bib25","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.3390\/ijms20092253","article-title":"C1 compound biosensors: design, functional study, and applications","volume":"20","author":"Lee","year":"2019","journal-title":"Int J Mol Sci"},{"issue":"17","key":"10.1016\/j.synbio.2023.05.004_bib26","doi-asserted-by":"crossref","first-page":"5076","DOI":"10.1128\/JB.185.17.5076-5085.2003","article-title":"Coordinate regulation of the Escherichia coli formate dehydrogenase fdnGHI and fdhF Genes in response to nitrate, nitrite, and formate: roles for NarL and NarP","volume":"185","author":"Wang","year":"2003","journal-title":"J Bacteriol"},{"issue":"1","key":"10.1016\/j.synbio.2023.05.004_bib27","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/s41467-020-20754-4","article-title":"Genetically encoded formaldehyde sensors inspired by a protein intra-helical crosslinking reaction","volume":"12","author":"Zhu","year":"2021","journal-title":"Nat Commun"},{"issue":"3","key":"10.1016\/j.synbio.2023.05.004_bib28","first-page":"416","article-title":"The colorimetric estimation of formaldehyde by means of the hantzsch reaction","volume":"55","author":"Nash","year":"1953","journal-title":"J Biol Chem"},{"issue":"1","key":"10.1016\/j.synbio.2023.05.004_bib29","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0160-5402(82)90004-3","article-title":"Sensitive formaldehyde determination with Nash's reagent and a 'Tryptophan reaction'","volume":"8","author":"Kleeberg","year":"1982","journal-title":"J Pharmacol Methods"},{"issue":"3","key":"10.1016\/j.synbio.2023.05.004_bib30","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.3390\/ijms22031471","article-title":"Biosensor-based directed evolution of methanol dehydrogenase from Lysinibacillus xylanilyticus","volume":"22","author":"Le","year":"2021","journal-title":"Int J Mol Sci"},{"issue":"4","key":"10.1016\/j.synbio.2023.05.004_bib31","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1021\/acssynbio.8b00481","article-title":"Phage-assisted evolution of Bacillus methanolicus methanol dehydrogenase 2","volume":"8","author":"Roth","year":"2019","journal-title":"ACS Synth Biol"},{"issue":"6786","key":"10.1016\/j.synbio.2023.05.004_bib32","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1038\/35014651","article-title":"Engineering stability in gene networks by autoregulation","volume":"405","author":"Becskei","year":"2000","journal-title":"Nature"},{"issue":"37","key":"10.1016\/j.synbio.2023.05.004_bib33","doi-asserted-by":"crossref","first-page":"19502","DOI":"10.1074\/jbc.M116.745174","article-title":"The effectors and sensory sites of formaldehyde-responsive regulator FrmR and metal-sensing variant","volume":"291","author":"Osman","year":"2016","journal-title":"J Biol Chem"},{"key":"10.1016\/j.synbio.2023.05.004_bib34","doi-asserted-by":"crossref","DOI":"10.1038\/srep38879","article-title":"The mechanism of a formaldehyde-sensing transcriptional regulator","volume":"6","author":"Denby","year":"2016","journal-title":"Sci Rep"},{"issue":"12","key":"10.1016\/j.synbio.2023.05.004_bib35","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.1016\/j.bmcl.2019.04.025","article-title":"Characterization of the substrate scope of an alcohol dehydrogenase commonly used as methanol dehydrogenase","volume":"29","author":"Guo","year":"2019","journal-title":"Bioorg Med Chem Lett"},{"issue":"7873","key":"10.1016\/j.synbio.2023.05.004_bib36","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.synbio.2023.05.004_bib37","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.ymben.2021.09.005","article-title":"Recent advances toward the bioconversion of methane and methanol in synthetic methylotrophs","volume":"71","author":"Gregory","year":"2022","journal-title":"Metab Eng"},{"key":"10.1016\/j.synbio.2023.05.004_bib38","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.copbio.2019.07.001","article-title":"Synthetic methylotrophy: strategies to assimilate methanol for growth and chemicals production","volume":"59","author":"Antoniewicz","year":"2019","journal-title":"Curr Opin Biotechnol"},{"issue":"10","key":"10.1016\/j.synbio.2023.05.004_bib39","doi-asserted-by":"crossref","first-page":"6815","DOI":"10.1021\/acs.analchem.9b01017","article-title":"A fluorescence-activated single-droplet dispenser for high accuracy single-droplet and single-cell sorting and dispensing","volume":"91","author":"Qin","year":"2019","journal-title":"Anal Chem"},{"key":"10.1016\/j.synbio.2023.05.004_bib40","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.ymben.2017.01.006","article-title":"Improving key enzyme activity in phenylpropanoid pathway with a designed biosensor","volume":"40","author":"Xiong","year":"2017","journal-title":"Metab Eng"},{"key":"10.1016\/j.synbio.2023.05.004_bib41","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.ymben.2021.10.003","article-title":"Biosensor-based growth-coupling and spatial separation as an evolution strategy to improve small molecule production of Corynebacterium glutamicum","volume":"68","author":"Stella","year":"2021","journal-title":"Metab Eng"}],"container-title":["Synthetic and Systems Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405805X23000340?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405805X23000340?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:03:46Z","timestamp":1762506226000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405805X23000340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["S2405805X23000340"],"URL":"https:\/\/doi.org\/10.1016\/j.synbio.2023.05.004","relation":{},"ISSN":["2405-805X"],"issn-type":[{"value":"2405-805X","type":"print"}],"subject":[],"published":{"date-parts":[[2023,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde","name":"articletitle","label":"Article Title"},{"value":"Synthetic and Systems Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.synbio.2023.05.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.","name":"copyright","label":"Copyright"}]}}