{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T07:05:49Z","timestamp":1787814349621,"version":"build-2784847793"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T00:00:00Z","timestamp":1768003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32102136"],"award-info":[{"award-number":["32102136"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22378156"],"award-info":[{"award-number":["22378156"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Synthetic and Systems Biotechnology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.synbio.2026.01.008","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T12:42:22Z","timestamp":1768912942000},"page":"1-13","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Engineering Kluyveromyces marxianus for 3-hydroxypropionic acid production at elevated temperature from Jerusalem artichoke tubers and crude glycerol"],"prefix":"10.1016","volume":"13","author":[{"given":"Jiacheng","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongmei","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjie","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingtao","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9627-7789","authenticated-orcid":false,"given":"Biao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.synbio.2026.01.008_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2022.127690","article-title":"Overproduction of 3-hydroxypropionate in a super yeast chassis","volume":"361","author":"Yu","year":"2022","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.synbio.2026.01.008_bib2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s11274-021-03091-6","article-title":"Biosynthesis pathways and strategies for improving 3-hydroxypropionic acid production in bacteria","volume":"37","author":"Zhao","year":"2021","journal-title":"World J Microbiol Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.008_bib3","doi-asserted-by":"crossref","first-page":"3236","DOI":"10.1021\/acssynbio.0c00264","article-title":"Rewiring central carbon metabolism ensures increased provision of Acetyl-CoA and NADPH required for 3-OH-Propionic acid production","volume":"9","author":"Qin","year":"2020","journal-title":"ACS Synth Biol"},{"key":"10.1016\/j.synbio.2026.01.008_bib4","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.synbio.2025.09.002","article-title":"Engineering Yarrowia lipolytica as a yeast cell factory for the de novo production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate)","volume":"11","author":"Liu","year":"2026","journal-title":"Synth Syst Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.008_bib5","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1021\/acssynbio.6b00346","article-title":"Increasing malonyl-CoA derived product through controlling the transcription regulators of phospholipid synthesis in Saccharomyces cerevisiae","volume":"6","author":"Chen","year":"2017","journal-title":"ACS Synth Biol"},{"key":"10.1016\/j.synbio.2026.01.008_bib6","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.3389\/fmicb.2018.02185","article-title":"Metabolic engineering of yeast for the production of 3-hydroxypropionic acid","volume":"9","author":"Ji","year":"2018","journal-title":"Front Microbiol"},{"key":"10.1016\/j.synbio.2026.01.008_bib7","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1021\/acssynbio.6b00007","article-title":"Metabolic engineering of Escherichia coli for the production of 3-Hydroxypropionic acid and malonic acid through beta-Alanine route","volume":"5","author":"Song","year":"2016","journal-title":"ACS Synth Biol"},{"key":"10.1016\/j.synbio.2026.01.008_bib8","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1016\/j.biotechadv.2018.03.020","article-title":"Process engineering for microbial production of 3-hydroxypropionic acid","volume":"36","author":"de Fouchecour","year":"2018","journal-title":"Biotechnol Adv"},{"key":"10.1016\/j.synbio.2026.01.008_bib9","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1186\/s12934-024-02546-3","article-title":"Altered sterol composition mediates multiple tolerance of Kluyveromyces marxianus for xylitol production","volume":"23","author":"Ren","year":"2024","journal-title":"Microb Cell Fact"},{"key":"10.1016\/j.synbio.2026.01.008_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2022.108027","article-title":"Kluyveromyces marxianus as a microbial cell factory for lignocellulosic biomass valorisation","volume":"60","author":"Baptista","year":"2022","journal-title":"Biotechnol Adv"},{"key":"10.1016\/j.synbio.2026.01.008_bib11","doi-asserted-by":"crossref","first-page":"5915","DOI":"10.1021\/jf3008629","article-title":"Characterization of crude glycerol from biodiesel plants","volume":"60","author":"Hu","year":"2012","journal-title":"J Agric Food Chem"},{"key":"10.1016\/j.synbio.2026.01.008_bib12","doi-asserted-by":"crossref","first-page":"15901","DOI":"10.1021\/acssuschemeng.4c03144","article-title":"Highly efficient production of ergothioneine from glycerol through gene mining and CRISPR-Cas9 synthetic biology technology in Saccharomyces cerevisiae","volume":"12","author":"Yu","year":"2024","journal-title":"ACS Sustain Chem Eng"},{"key":"10.1016\/j.synbio.2026.01.008_bib13","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.biortech.2014.10.150","article-title":"Improving xylitol production at elevated temperature with engineered Kluyveromyces marxianus through over-expressing transporters","volume":"175","author":"Zhang","year":"2015","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.synbio.2026.01.008_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2022.127878","article-title":"Ergosterol production at elevated temperatures by Upc2-overexpressing Kluyveromyces marxianus using Jerusalem artichoke tubers as feedstock","volume":"362","author":"Liu","year":"2022","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.synbio.2026.01.008_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2022.128179","article-title":"Improving glycerol utilization during high-temperature xylitol production with Kluyveromyces marxianus using a transient clustered regularly interspaced short palindromic repeats (CRISPR)\/CRISPR-associated protein 9 system","volume":"365","author":"Ren","year":"2022","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.synbio.2026.01.008_bib35","doi-asserted-by":"crossref","first-page":"7991","DOI":"10.1007\/s00253-020-10808-4","article-title":"Functional analysis of PGI1 and ZWF1 in thermotolerant yeast Kluyveromyces marxianus","volume":"104","author":"Zhang","year":"2020","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.008_bib16","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.synbio.2025.09.015","article-title":"Development of a CRISPR\/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis","volume":"11","author":"Zha","year":"2026","journal-title":"Synth Syst Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.008_bib14","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.ymben.2015.07.008","article-title":"Rapid ethanol production at elevated temperatures by engineered thermotolerant Kluyveromyces marxianus via the NADP(H)-preferring xylose reductase-xylitol dehydrogenase pathway","volume":"31","author":"Zhang","year":"2015","journal-title":"Metab Eng"},{"key":"10.1016\/j.synbio.2026.01.008_bib17","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.biortech.2016.05.068","article-title":"Simultaneous fermentation of glucose and xylose at elevated temperatures co-produces ethanol and xylitol through overexpression of a xylose-specific transporter in engineered Kluyveromyces marxianus","volume":"216","author":"Zhang","year":"2016","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.synbio.2026.01.008_bib18","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/j.biortech.2016.11.110","article-title":"Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose","volume":"224","author":"Zhang","year":"2017","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.synbio.2026.01.008_bib19","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s13068-023-02309-z","article-title":"Engineering yeast mitochondrial metabolism for 3-hydroxypropionate production","volume":"16","author":"Zhang","year":"2023","journal-title":"Biotechnol Biofuels Bioprod"},{"key":"10.1016\/j.synbio.2026.01.008_bib20","doi-asserted-by":"crossref","first-page":"6445","DOI":"10.1021\/acssuschemeng.3c00410","article-title":"Efficient bioproduction of 3-Hydroxypropionic acid from methanol by a synthetic yeast cell factory","volume":"11","author":"Wu","year":"2023","journal-title":"ACS Sustain Chem Eng"},{"key":"10.1016\/j.synbio.2026.01.008_bib22","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1007\/s00253-019-09637-x","article-title":"Understanding the stress responses of Kluyveromyces marxianus after an arrest during high-temperature ethanol fermentation based on integration of RNA-Seq and metabolite data","volume":"103","author":"Fu","year":"2019","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.008_bib23","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/1754-6834-6-87","article-title":"Identification of multiple interacting alleles conferring low glycerol and high ethanol yield in Saccharomyces cerevisiae ethanolic fermentation","volume":"6","author":"Hubmann","year":"2013","journal-title":"Biotechnol Biofuels"},{"key":"10.1016\/j.synbio.2026.01.008_bib24","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1074\/jbc.M203030200","article-title":"Dihydroxyacetone kinases in Saccharomyces cerevisiae are involved in detoxification of dihydroxyacetone","volume":"278","author":"Molin","year":"2003","journal-title":"J Biol Chem"},{"key":"10.1016\/j.synbio.2026.01.008_bib25","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1111\/jam.12013","article-title":"Characterization of GCY1 in Saccharomyces cerevisiae by metabolic profiling","volume":"113","author":"Jung","year":"2012","journal-title":"J Appl Microbiol"},{"key":"10.1016\/j.synbio.2026.01.008_bib26","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.ymben.2016.11.009","article-title":"Metabolic engineering of Corynebacterium glutamicum for the production of 3-hydroxypropionic acid from glucose and xylose","volume":"39","author":"Chen","year":"2017","journal-title":"Metab Eng"},{"key":"10.1016\/j.synbio.2026.01.008_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2019.122600","article-title":"Improved production of 3-hydroxypropionic acid in engineered Escherichia coli by rebalancing heterologous and endogenous synthetic pathways","volume":"299","author":"Lee","year":"2020","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.synbio.2026.01.008_bib28","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.ymben.2018.04.007","article-title":"Iterative genome editing of Escherichia coli for 3-hydroxypropionic acid production","volume":"47","author":"Liu","year":"2018","journal-title":"Metab Eng"},{"key":"10.1016\/j.synbio.2026.01.008_bib29","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.synbio.2024.06.003","article-title":"Engineering Corynebacterium glutamicum for the efficient production of 3-hydroxypropionic acid from glucose via the \u03b2-alanine pathway","volume":"9","author":"Wang","year":"2024","journal-title":"Synth Syst Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.008_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2019.121320","article-title":"Enhanced production of 3-hydroxypropionic acid from glucose and xylose by alleviation of metabolic congestion due to glycerol flux in engineered Escherichia coli","volume":"285","author":"Heo","year":"2019","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.synbio.2026.01.008_bib31","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s13036-025-00488-x","article-title":"Metabolic engineering of Komagataella phaffii for enhanced 3-hydroxypropionic acid (3-HP) production from methanol","volume":"19","author":"Avila-Cabr\u00e9","year":"2025","journal-title":"J Biol Eng"},{"key":"10.1016\/j.synbio.2026.01.008_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2025.132656","article-title":"Expanding the genetic toolkit of Yarrowia lipolytica: dynamic promoter engineering enables high-titer biosynthesis of 3-hydroxypropionic acid","volume":"432","author":"Kang","year":"2025","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.synbio.2026.01.008_bib33","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1016\/j.synbio.2025.04.013","article-title":"Promoter engineering for enhanced 3-hydroxypropionic acid production in Pichia pastoris","volume":"10","author":"Chen","year":"2025","journal-title":"Synth Syst Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.008_bib34","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1016\/j.synbio.2025.05.011","article-title":"Enhancing 3-hydroxypropionic acid production from recombinant Saccharomyces cerevisiae for using rice straw hydrolysate and sugar cane industrial waste as substrate","volume":"10","author":"Lertsriwong","year":"2025","journal-title":"Synth Syst Biotechnol"}],"container-title":["Synthetic and Systems Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405805X26000086?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405805X26000086?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:27:52Z","timestamp":1786994872000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405805X26000086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":35,"alternative-id":["S2405805X26000086"],"URL":"https:\/\/doi.org\/10.1016\/j.synbio.2026.01.008","relation":{},"ISSN":["2405-805X"],"issn-type":[{"value":"2405-805X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Engineering Kluyveromyces marxianus for 3-hydroxypropionic acid production at elevated temperature from Jerusalem artichoke tubers and crude glycerol","name":"articletitle","label":"Article Title"},{"value":"Synthetic and Systems Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.synbio.2026.01.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.","name":"copyright","label":"Copyright"}]}}