{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T20:30:14Z","timestamp":1786998614899,"version":"build-2736575974"},"reference-count":62,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T00:00:00Z","timestamp":1768608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013290","name":"National Key Research and Development Program of China Stem Cell and Translational Research","doi-asserted-by":"publisher","award":["2023YFD1700700"],"award-info":[{"award-number":["2023YFD1700700"]}],"id":[{"id":"10.13039\/501100013290","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32370077"],"award-info":[{"award-number":["32370077"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Synthetic and Systems Biotechnology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.synbio.2026.01.015","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T09:34:56Z","timestamp":1769765696000},"page":"98-108","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["High-level prodigiosin production in Pseudomonas putida enabled by combinatorial metabolic engineering"],"prefix":"10.1016","volume":"13","author":[{"given":"Yuxin","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiyan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaijie","family":"Dou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruizhi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunfang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Bian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Si","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4796-5630","authenticated-orcid":false,"given":"Guoqing","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.synbio.2026.01.015_bib1","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1038\/nrmicro1531","article-title":"The biosynthesis and regulation of bacterial prodiginines","volume":"4","author":"Williamson","year":"2006","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.synbio.2026.01.015_bib2","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1021\/ja01487a075","article-title":"Prodigiosin structure and partial synthesis","volume":"82","author":"Wasserman","year":"1960","journal-title":"J Am Chem Soc"},{"key":"10.1016\/j.synbio.2026.01.015_bib3","doi-asserted-by":"crossref","first-page":"7721","DOI":"10.1007\/s00253-022-12245-x","article-title":"Structures, biosynthesis, and bioactivities of prodiginine natural products","volume":"106","author":"Li","year":"2022","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib4","doi-asserted-by":"crossref","first-page":"3841","DOI":"10.1007\/s00253-014-5590-1","article-title":"Properties and applications of undecylprodigiosin and other bacterial prodigiosins","volume":"98","author":"Stankovic","year":"2014","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib5","first-page":"4967","article-title":"Exceptional production of both prodigiosin and cycloprodigiosin as major metabolic constituents by a novel marine bacterium, Zooshikella rubidus S1-1","volume":"77","author":"Lee","year":"2011","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib6","doi-asserted-by":"crossref","first-page":"4626","DOI":"10.1021\/acsomega.9b04322","article-title":"An Indonesian marine bacterium, Pseudoalteromonas rubra, produces antimicrobial prodiginine pigments","volume":"5","author":"Setiyono","year":"2020","journal-title":"ACS Omega"},{"key":"10.1016\/j.synbio.2026.01.015_bib7","first-page":"937","article-title":"Biosynthesis of antibiotic prodiginines in the marine bacterium Hahella chejuensis KCTC 2396","volume":"102","author":"Kim","year":"2007","journal-title":"J Appl Microbiol"},{"key":"10.1016\/j.synbio.2026.01.015_bib8","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1080\/14786419.2016.1195380","article-title":"Antimicrobial activity of prodigiosin is attributable to plasma-membrane damage","volume":"31","author":"Suryawanshi","year":"2016","journal-title":"Nat Prod Res"},{"key":"10.1016\/j.synbio.2026.01.015_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2019.113444","article-title":"Simultaneous Microcystis algicidal and microcystin synthesis inhibition by a red pigment prodigiosin","volume":"256","author":"Wei","year":"2020","journal-title":"Environ Pollut"},{"key":"10.1016\/j.synbio.2026.01.015_bib10","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.exppara.2004.04.007","article-title":"Isolation of Serratia marcescens in the midgut of Rhodnius prolixus: impact on the establishment of the parasite Trypanosoma cruzi in the vector","volume":"107","author":"Azambuja","year":"2004","journal-title":"Exp Parasitol"},{"key":"10.1016\/j.synbio.2026.01.015_bib11","doi-asserted-by":"crossref","first-page":"5393","DOI":"10.1007\/s13197-015-1740-4","article-title":"Prodigiosin and its potential applications","volume":"52","author":"Darshan","year":"2015","journal-title":"J Food Sci Technol"},{"key":"10.1016\/j.synbio.2026.01.015_bib12","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1016\/j.semcancer.2021.05.006","article-title":"Bacteria as a treasure house of secondary metabolites with anticancer potential","volume":"86","author":"Mohan","year":"2022","journal-title":"Semin Cancer Biol"},{"key":"10.1016\/j.synbio.2026.01.015_bib13","doi-asserted-by":"crossref","first-page":"3547","DOI":"10.1099\/mic.0.27222-0","article-title":"The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation","volume":"150","author":"Harris","year":"2004","journal-title":"Microbiology"},{"key":"10.1016\/j.synbio.2026.01.015_bib14","doi-asserted-by":"crossref","first-page":"6874","DOI":"10.1021\/ja00801a080","article-title":"Biosynthesis of prodigiosin. Incorporation patterns of C-labeled alanine, proline, glycine, and serine elucidated by fourier transform nuclear magnetic resonance","volume":"95","author":"Wasserman","year":"1973","journal-title":"J Am Chem Soc"},{"key":"10.1016\/j.synbio.2026.01.015_bib15","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1128\/am.25.3.396-402.1973","article-title":"Biosynthesis of prodigiosin, a secondary metabolite of Serratia marcescens","volume":"25","author":"Williams","year":"1973","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.synbio.2026.01.015_bib16","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1021\/acs.biochem.0c00830","article-title":"Solution structure and conformational dynamics of a doublet acyl carrier protein from prodigiosin biosynthesis","volume":"60","author":"Thongkawphueak","year":"2021","journal-title":"Biochemistry"},{"key":"10.1016\/j.synbio.2026.01.015_bib17","doi-asserted-by":"crossref","first-page":"12600","DOI":"10.1021\/ja063611l","article-title":"Protein assembly line components in prodigiosin biosynthesis: characterization of PigA,G,H,I,J","volume":"128","author":"Garneau-Tsodikova","year":"2006","journal-title":"J Am Chem Soc"},{"key":"10.1016\/j.synbio.2026.01.015_bib18","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.3389\/fmicb.2019.01715","article-title":"Biosynthesis and bioactivity of prodiginine analogs in marine bacteria, Pseudoalteromonas: a mini review","volume":"10","author":"Sakai-Kawada","year":"2019","journal-title":"Front Microbiol"},{"key":"10.1016\/j.synbio.2026.01.015_bib19","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1146\/annurev-chembioeng-061010-114209","article-title":"Metabolic engineering for the production of natural products","volume":"2","author":"Pickens","year":"2011","journal-title":"Annu Rev Chem Biomol Eng"},{"key":"10.1016\/j.synbio.2026.01.015_bib20","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1039\/C8NP00091C","article-title":"Heterologous expression of bacterial natural product biosynthetic pathways","volume":"36","author":"Huo","year":"2019","journal-title":"Nat Prod Rep"},{"key":"10.1016\/j.synbio.2026.01.015_bib21","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1016\/j.tibtech.2019.01.003","article-title":"Systems metabolic engineering strategies: integrating systems and synthetic biology with metabolic engineering","volume":"37","author":"Choi","year":"2019","journal-title":"Trends Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib22","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1128\/AEM.01468-09","article-title":"Genome-wide screening and identification of factors affecting the biosynthesis of prodigiosin by Hahella chejuensis, using Escherichia coli as a surrogate host","volume":"76","author":"Kwon","year":"2010","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.synbio.2026.01.015_bib23","first-page":"539","article-title":"Biosynthesis of carbapenem antibiotic and prodigiosin pigment in Serratia is under quorum sensing control","volume":"36","author":"Thomson","year":"2000","journal-title":"Mol Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib24","first-page":"4290","article-title":"Isolation, identification, and genomic analysis of a prodigiosin-producing strain of Serratia marcescens","volume":"64","author":"Zhang","year":"2024","journal-title":"Acta Microbiol Sin"},{"key":"10.1016\/j.synbio.2026.01.015_bib25","doi-asserted-by":"crossref","first-page":"972","DOI":"10.3389\/fmicb.2015.00972","article-title":"Efficient recombinant production of prodigiosin in Pseudomonas putida","volume":"6","author":"Domrose","year":"2015","journal-title":"Front Microbiol"},{"key":"10.1016\/j.synbio.2026.01.015_bib26","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.synbio.2017.11.001","article-title":"Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX","volume":"2","author":"Domrose","year":"2017","journal-title":"Synth Syst Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib27","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1021\/sb3000657","article-title":"TREX: a universal tool for the transfer and expression of biosynthetic pathways in bacteria","volume":"2","author":"Loeschcke","year":"2012","journal-title":"ACS Synth Biol"},{"key":"10.1016\/j.synbio.2026.01.015_bib28","article-title":"Exploring engineered vesiculation by Pseudomonas putida KT2440 for natural product biosynthesis","volume":"17","author":"Bitzenhofer","year":"2023","journal-title":"Microb Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib29","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.ymben.2021.06.004","article-title":"Stepwise genetic engineering of Pseudomonas putida enables robust heterologous production of prodigiosin and glidobactin A","volume":"67","author":"Cook","year":"2021","journal-title":"Metab Eng"},{"key":"10.1016\/j.synbio.2026.01.015_bib30","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1073\/pnas.0337542100","article-title":"PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin","volume":"100","author":"Gust","year":"2003","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.synbio.2026.01.015_bib31","doi-asserted-by":"crossref","DOI":"10.1186\/s12934-018-0887-x","article-title":"Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system","volume":"17","author":"Sun","year":"2018","journal-title":"Microb Cell Fact"},{"key":"10.1016\/j.synbio.2026.01.015_bib32","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.ymben.2021.11.010","article-title":"Engineering of Corynebacterium glutamicum for high-level \u03b3-aminobutyric acid production from glycerol by dynamic metabolic control","volume":"69","author":"Wei","year":"2022","journal-title":"Metab Eng"},{"key":"10.1016\/j.synbio.2026.01.015_bib33","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1038\/s41929-023-01103-2","article-title":"A non-carboxylative route for the efficient synthesis of central metabolite malonyl-CoA and its derived products","volume":"7","author":"Li","year":"2024","journal-title":"Nat Catal"},{"key":"10.1016\/j.synbio.2026.01.015_bib34","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1186\/s13568-017-0495-x","article-title":"Linear and exponential TAIL-PCR: a method for efficient and quick amplification of flanking sequences adjacent to Tn5 transposon insertion sites","volume":"7","author":"Jia","year":"2017","journal-title":"AMB Express"},{"key":"10.1016\/j.synbio.2026.01.015_bib35","doi-asserted-by":"crossref","first-page":"649","DOI":"10.2144\/000112601","article-title":"High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences","volume":"43","author":"Liu","year":"2007","journal-title":"Biotechniques"},{"key":"10.1016\/j.synbio.2026.01.015_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2024.150597","article-title":"Design a reducing CO2 emission system using nonfermentable substrates for carbon-economic biosynthesis of poly-2-hydrobutanedioic acid","volume":"487","author":"He","year":"2024","journal-title":"Chem Eng J"},{"key":"10.1016\/j.synbio.2026.01.015_bib37","doi-asserted-by":"crossref","first-page":"W29","DOI":"10.1093\/nar\/gkab335","article-title":"antiSMASH 6.0: improving cluster detection and comparison capabilities","volume":"49","author":"Blin","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.synbio.2026.01.015_bib38","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1093\/bioinformatics\/btr039","article-title":"Easyfig: a genome comparison visualizer","volume":"27","author":"Sullivan","year":"2011","journal-title":"Bioinformatics"},{"key":"10.1016\/j.synbio.2026.01.015_bib39","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1038\/35097083","article-title":"Genome sequence of Yersinia pestis, the causative agent of plague","volume":"413","author":"Parkhill","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.synbio.2026.01.015_bib40","article-title":"Discovery of recombinases enables genome mining of cryptic biosynthetic gene clusters in Burkholderiales species","volume":"115","author":"Wang","year":"2018","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.synbio.2026.01.015_bib41","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1021\/acssynbio.5b00058","article-title":"Tn7-based device for calibrated heterologous gene expression in Pseudomonas putida","volume":"4","author":"Zobel","year":"2015","journal-title":"ACS Synth Biol"},{"key":"10.1016\/j.synbio.2026.01.015_bib42","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2020.124239","article-title":"Metabolic engineering of Pseudomonas putida KT2440 for high-yield production of protocatechuic acid","volume":"319","author":"Li","year":"2021","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.synbio.2026.01.015_bib43","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/j.ijbiomac.2021.09.142","article-title":"A promoter engineering-based strategy enhances polyhydroxyalkanoate production in Pseudomonas putida KT2440","volume":"191","author":"Zhang","year":"2021","journal-title":"Int J Biol Macromol"},{"key":"10.1016\/j.synbio.2026.01.015_bib44","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-97550-7","article-title":"Inducible and tunable gene expression systems for Pseudomonas putida KT2440","volume":"11","author":"Sathesh-Prabu","year":"2021","journal-title":"Sci Rep"},{"key":"10.1016\/j.synbio.2026.01.015_bib45","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1111\/1751-7915.13455","article-title":"Cell density\u2010dependent auto\u2010inducible promoters for expression of recombinant proteins in Pseudomonas putida","volume":"12","author":"Meyers","year":"2019","journal-title":"Microb Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib46","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.02889-16","article-title":"Large-scale transposition mutagenesis of Streptomyces coelicolor identifies hundreds of genes influencing antibiotic biosynthesis","volume":"83","author":"Xu","year":"2017","journal-title":"Appl Environ Microb"},{"key":"10.1016\/j.synbio.2026.01.015_bib47","doi-asserted-by":"crossref","first-page":"7451","DOI":"10.1007\/s00018-021-03996-3","article-title":"Dihydrolipoamide dehydrogenase, pyruvate oxidation, and acetylation-dependent mechanisms intersecting drug iatrogenesis","volume":"78","author":"Duarte","year":"2021","journal-title":"Cell Mol Life Sci"},{"key":"10.1016\/j.synbio.2026.01.015_bib48","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.carres.2017.04.003","article-title":"Identification and biochemical characterization of a novel \u03b1-1,3-mannosyltransferase WfcD from Escherichia coli O141","volume":"443\u2013444","author":"Chen","year":"2017","journal-title":"Carbohydr Res"},{"key":"10.1016\/j.synbio.2026.01.015_bib49","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1002\/bab.2170","article-title":"Bacterial pigments: a colorful palette reservoir for biotechnological applications","volume":"69","author":"Orlandi","year":"2021","journal-title":"Biotechnol Appl Biochem"},{"key":"10.1016\/j.synbio.2026.01.015_bib50","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1007\/s13399-020-00928-2","article-title":"A comprehensive review on recent trends in production, purification, and applications of prodigiosin","volume":"12","author":"Paul","year":"2020","journal-title":"Biomass Conv Bioref"},{"key":"10.1016\/j.synbio.2026.01.015_bib51","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s13213-014-0837-8","article-title":"Physical and chemical stress on Serratia marcescens and studies on prodigiosin pigment production","volume":"65","author":"El-Bialy","year":"2014","journal-title":"Ann Microbiol"},{"key":"10.1016\/j.synbio.2026.01.015_bib52","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1080\/10826068.2012.721850","article-title":"Mutant breeding of Serratia marcescens strain for enhancing prodigiosin production and application to textiles","volume":"43","author":"Liu","year":"2013","journal-title":"Prep Biochem Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib53","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.00543-21","article-title":"Enhanced prodigiosin production in Serratia marcescens JNB5-1 by introduction of a polynucleotide fragment into the pigN 3\u2032 untranslated region and disulfide bonds into O-methyl transferase (PigF)","volume":"87","author":"Sun","year":"2021","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.synbio.2026.01.015_bib54","first-page":"549","article-title":"Peanut oil cake: a novel substrate for enhanced cell growth and prodigiosin production from Serratia marcescens CF-53","volume":"2","author":"Naik","year":"2012","journal-title":"J Res Biol"},{"key":"10.1016\/j.synbio.2026.01.015_bib55","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/s12010-011-9415-8","article-title":"Characterization and enhanced production of prodigiosin from the spoiled coconut","volume":"166","author":"Siva","year":"2012","journal-title":"Appl Biochem Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib56","doi-asserted-by":"crossref","first-page":"6197","DOI":"10.1007\/s00253-015-6745-4","article-title":"Pseudomonas putida-a versatile host for the production of natural products","volume":"99","author":"Loeschcke","year":"2015","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib57","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1111\/j.1365-2958.2005.04602.x","article-title":"Biosynthesis of the red antibiotic, prodigiosin, in Serratia: identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces","volume":"56","author":"Williamson","year":"2005","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.synbio.2026.01.015_bib58","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1128\/jb.106.2.444-448.1971","article-title":"Induction of pigmentation in nonproliferating cells of Serratia marcescens by addition of single amino acids","volume":"106","author":"Williams","year":"1971","journal-title":"J Bacteriol"},{"key":"10.1016\/j.synbio.2026.01.015_bib59","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1128\/aem.31.1.70-77.1976","article-title":"Macromolecular syntheses during biosynthesis of prodigiosin by Serratia marcescens","volume":"31","author":"Williams","year":"1976","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.synbio.2026.01.015_bib60","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s43141-020-00045-7","article-title":"Optimization of prodigiosin biosynthesis by Serratia marcescens using unconventional bioresources","volume":"18","author":"Bhagwat","year":"2020","journal-title":"J Genet Eng Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib61","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1007\/s11274-004-7257-z","article-title":"Strategy for the improvement of prodigiosin production by a Serratia marcescens mutant through fed-batch fermentation","volume":"21","author":"Tao","year":"2005","journal-title":"World J Microbiol Biotechnol"},{"key":"10.1016\/j.synbio.2026.01.015_bib62","doi-asserted-by":"crossref","first-page":"15215","DOI":"10.1074\/jbc.M113.544080","article-title":"Structure and function of the catalytic domain of the dihydrolipoyl acetyltransferase component in Escherichia coli pyruvate dehydrogenase complex","volume":"289","author":"Wang","year":"2014","journal-title":"J Biol Chem"}],"container-title":["Synthetic and Systems Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405805X26000207?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405805X26000207?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:28:54Z","timestamp":1786994934000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405805X26000207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":62,"alternative-id":["S2405805X26000207"],"URL":"https:\/\/doi.org\/10.1016\/j.synbio.2026.01.015","relation":{},"ISSN":["2405-805X"],"issn-type":[{"value":"2405-805X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"High-level prodigiosin production in Pseudomonas putida enabled by combinatorial metabolic engineering","name":"articletitle","label":"Article Title"},{"value":"Synthetic and Systems Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.synbio.2026.01.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.","name":"copyright","label":"Copyright"}]}}