{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T20:29:32Z","timestamp":1786998572872,"version":"build-2736575974"},"reference-count":62,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T00:00:00Z","timestamp":1771113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32471277"],"award-info":[{"award-number":["32471277"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Shandong Province Natural Science Foundation","doi-asserted-by":"publisher","award":["ZR2023MC066"],"award-info":[{"award-number":["ZR2023MC066"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000148","name":"Canadian Anesthesiologists' Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000148","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFA0916100"],"award-info":[{"award-number":["2023YFA0916100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Synthetic and Systems Biotechnology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.synbio.2026.02.009","type":"journal-article","created":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T05:57:02Z","timestamp":1772863022000},"page":"337-349","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Engineered eMutS based magnetic separation system for high-fidelity DNA synthesis"],"prefix":"10.1016","volume":"13","author":[{"given":"Xiaohang","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7228-7712","authenticated-orcid":false,"given":"Yefei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenshuang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengsha","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingying","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changle","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Sheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2063-2385","authenticated-orcid":false,"given":"Jia","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"11","key":"10.1016\/j.synbio.2026.02.009_bib1","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1038\/nmeth.2894","article-title":"Programming biological operating systems: genome design, assembly and activation","author":"Gibson","year":"2014","journal-title":"Nat Methods"},{"key":"10.1016\/j.synbio.2026.02.009_bib2","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1146\/annurev-biochem-060713-035418","article-title":"Genome engineering with targetable nucleases","volume":"83","author":"Carroll","year":"2014","journal-title":"Annu Rev Biochem"},{"key":"10.1016\/j.synbio.2026.02.009_bib3","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.nbt.2025.05.002","article-title":"MutS-mediated rapid and cost-effective error correction in in vitro DNA synthesis","volume":"88","author":"Jia","year":"2025","journal-title":"N Biotech"},{"issue":"5987","key":"10.1016\/j.synbio.2026.02.009_bib4","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1126\/science.1190719","article-title":"Creation of a bacterial cell controlled by a chemically synthesized genome","volume":"329","author":"Gibson","year":"2010","journal-title":"Science"},{"issue":"5","key":"10.1016\/j.synbio.2026.02.009_bib5","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1038\/nrg2775","article-title":"Synthetic biology: applications come of age","volume":"11","author":"Khalil","year":"2010","journal-title":"Nat Rev Genet"},{"issue":"3","key":"10.1016\/j.synbio.2026.02.009_bib6","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.tibtech.2011.10.002","article-title":"Error correction in gene synthesis technology","volume":"30","author":"Ma","year":"2012","journal-title":"Trends Biotechnol"},{"issue":"1","key":"10.1016\/j.synbio.2026.02.009_bib7","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a023812","article-title":"Synthetic DNA synthesis and assembly: putting the synthetic in synthetic biology","volume":"9","author":"Hughes","year":"2017","journal-title":"Cold Spring Harbor Perspect Biol"},{"issue":"2","key":"10.1016\/j.synbio.2026.02.009_bib8","doi-asserted-by":"crossref","first-page":"116","DOI":"10.3390\/genes11020116","article-title":"Current and emerging methods for the synthesis of single-stranded DNA","volume":"11","author":"Hao","year":"2020","journal-title":"Genes"},{"issue":"4","key":"10.1016\/j.synbio.2026.02.009_bib9","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1021\/acssynbio.0c00079","article-title":"Efficient and low-cost error removal in DNA synthesis by a high-durability MutS","volume":"9","author":"Zhang","year":"2020","journal-title":"ACS Synth Biol"},{"issue":"1","key":"10.1016\/j.synbio.2026.02.009_bib10","doi-asserted-by":"crossref","first-page":"A.3C.1","DOI":"10.1002\/0471142700.nca03cs00","article-title":"Introduction to the synthesis and purification of oligonucleotides","volume":"00","author":"Ellington","year":"2000","journal-title":"Curr Protoc Nucleic Acid Chem"},{"key":"10.1016\/j.synbio.2026.02.009_bib11","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-022-16222-2","article-title":"Quantification of synthetic errors during chemical synthesis of DNA and its suppression by non-canonical nucleosides","volume":"12","author":"Masaki","year":"2022","journal-title":"Sci Rep"},{"issue":"7","key":"10.1016\/j.synbio.2026.02.009_bib12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0181128","article-title":"Correction: examining sources of error in PCR by single-molecule sequencing","volume":"12","author":"Potapov","year":"2017","journal-title":"PLoS One"},{"issue":"15","key":"10.1016\/j.synbio.2026.02.009_bib13","doi-asserted-by":"crossref","first-page":"9206","DOI":"10.1093\/nar\/gkx691","article-title":"A systematic comparison of error correction enzymes by next-generation sequencing","volume":"45","author":"Lubock","year":"2017","journal-title":"Nucleic Acids Res"},{"issue":"25","key":"10.1016\/j.synbio.2026.02.009_bib14","doi-asserted-by":"crossref","first-page":"3613","DOI":"10.1039\/D0CC00222D","article-title":"Stabilizing synthetic DNA for long-term data storage with Earth alkaline salts","volume":"56","author":"Kohll","year":"2020","journal-title":"Chem Commun"},{"issue":"1","key":"10.1016\/j.synbio.2026.02.009_bib15","doi-asserted-by":"crossref","first-page":"9663","DOI":"10.1038\/s41598-019-45832-6","article-title":"A characterization of the DNA data storage channel","volume":"9","author":"Heckel","year":"2019","journal-title":"Sci Rep"},{"key":"10.1016\/j.synbio.2026.02.009_bib16","doi-asserted-by":"crossref","first-page":"10.5.1","DOI":"10.1002\/0471142700.nc1005s61","article-title":"Analysis and purification of synthetic nucleic acids using HPLC","volume":"61","author":"Sinha","year":"2015","journal-title":"Curr Protoc Nucleic Acid Chem"},{"issue":"8","key":"10.1016\/j.synbio.2026.02.009_bib17","doi-asserted-by":"crossref","first-page":"2632","DOI":"10.1093\/nar\/gkh599","article-title":"Mismatch cleavage by single-strand specific nucleases","volume":"32","author":"Till","year":"2004","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"10.1016\/j.synbio.2026.02.009_bib18","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gni058","article-title":"Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage","volume":"33","author":"Fuhrmann","year":"2005","journal-title":"Nucleic Acids Res"},{"issue":"5","key":"10.1016\/j.synbio.2026.02.009_bib19","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1111\/j.1574-6976.2003.tb00626.x","article-title":"Single-strand-specific nucleases","volume":"26","author":"Desai","year":"2003","journal-title":"FEMS Microbiol Rev"},{"issue":"5","key":"10.1016\/j.synbio.2026.02.009_bib20","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1038\/nbt.1847","article-title":"Parallel on-chip gene synthesis and application to optimization of protein expression","volume":"29","author":"Quan","year":"2011","journal-title":"Nat Biotechnol"},{"issue":"12","key":"10.1016\/j.synbio.2026.02.009_bib21","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1038\/nbt.1716","article-title":"Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips","volume":"28","author":"Kosuri","year":"2010","journal-title":"Nat Biotechnol"},{"issue":"12","key":"10.1016\/j.synbio.2026.02.009_bib22","doi-asserted-by":"crossref","first-page":"6765","DOI":"10.1073\/pnas.121183298","article-title":"In vivo requirement for RecJ, ExoVII, ExoI, and ExoX in methyl-directed mismatch repair","volume":"98","author":"Burdett","year":"2001","journal-title":"Proc Natl Acad Sci USA"},{"issue":"17","key":"10.1016\/j.synbio.2026.02.009_bib23","doi-asserted-by":"crossref","first-page":"5692","DOI":"10.1093\/nar\/gkq350","article-title":"A novel single-stranded DNA-specific 3\u2019-5\u2019 exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways","volume":"38","author":"Shimada","year":"2010","journal-title":"Nucleic Acids Res"},{"issue":"15","key":"10.1016\/j.synbio.2026.02.009_bib24","doi-asserted-by":"crossref","first-page":"7556","DOI":"10.1093\/nar\/gkt495","article-title":"Recognition and processing of double-stranded DNA by ExoX, a distributive 3\u2019-5\u2019 exonuclease","volume":"41","author":"Wang","year":"2013","journal-title":"Nucleic Acids Res"},{"issue":"20","key":"10.1016\/j.synbio.2026.02.009_bib25","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gnh160","article-title":"Protein-mediated error correction for de novo DNA synthesis","volume":"32","author":"Carr","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.synbio.2026.02.009_bib26","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.mrrev.2017.07.001","article-title":"DNA mismatch repair and its many roles in eukaryotic cells","volume":"773","author":"Liu","year":"2017","journal-title":"Mutat Res Rev Mutat Res"},{"issue":"6","key":"10.1016\/j.synbio.2026.02.009_bib27","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gni053","article-title":"Correcting errors in synthetic DNA through consensus shuffling","volume":"33","author":"Binkowski","year":"2005","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"10.1016\/j.synbio.2026.02.009_bib28","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1006\/bbrc.1997.6551","article-title":"Analysis of the mismatch and insertion\/deletion binding properties of Thermus thermophilus, HB8, MutS","volume":"233","author":"Whitehouse","year":"1997","journal-title":"Biochem Biophys Res Commun"},{"issue":"Pt 3","key":"10.1016\/j.synbio.2026.02.009_bib29","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1042\/bj3540627","article-title":"Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches","volume":"354","author":"Brown","year":"2001","journal-title":"Biochem J"},{"issue":"7","key":"10.1016\/j.synbio.2026.02.009_bib30","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1016\/j.bios.2006.06.010","article-title":"A simple fluorescent method for detecting mismatched DNAs using a MutS-fluorophore conjugate","volume":"22","author":"Cho","year":"2007","journal-title":"Biosens Bioelectron"},{"issue":"12","key":"10.1016\/j.synbio.2026.02.009_bib31","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gku405","article-title":"Error removal in microchip-synthesized DNA using immobilized MutS","volume":"42","author":"Wan","year":"2014","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"10.1016\/j.synbio.2026.02.009_bib32","doi-asserted-by":"crossref","first-page":"5145","DOI":"10.1021\/acscatal.8b00364","article-title":"Enzyme engineering based on X-ray structures and kinetic profiling of substrate libraries: alcohol dehydrogenases for stereospecific synthesis of a broad range of chiral alcohols","volume":"8","author":"Nie","year":"2018","journal-title":"ACS Catal"},{"issue":"1","key":"10.1016\/j.synbio.2026.02.009_bib33","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.jbiosc.2014.05.028","article-title":"Alteration of the substrate specificity of cytochrome P450 CYP199A2 by site-directed mutagenesis","volume":"119","author":"Furuya","year":"2015","journal-title":"J Biosci Bioeng"},{"issue":"12","key":"10.1016\/j.synbio.2026.02.009_bib34","doi-asserted-by":"crossref","first-page":"8264","DOI":"10.1021\/acscatal.6b02159","article-title":"A single point mutation alters the transglycosylation\/hydrolysis partition, significantly enhancing the synthetic capability of an endo-glycoceramidase","volume":"6","author":"Durand","year":"2016","journal-title":"ACS Catal"},{"issue":"34","key":"10.1016\/j.synbio.2026.02.009_bib35","doi-asserted-by":"crossref","first-page":"10158","DOI":"10.1021\/ja036707d","article-title":"Conversion of a PLP-dependent racemase into an aldolase by a single active site mutation","volume":"125","author":"Seebeck","year":"2003","journal-title":"J Am Chem Soc"},{"issue":"2","key":"10.1016\/j.synbio.2026.02.009_bib36","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1093\/glycob\/cww081","article-title":"Rational design of a thermostable glycoside hydrolase from family 3 introduces \u03b2-glycosynthase activity","volume":"27","author":"Pozzo","year":"2017","journal-title":"Glycobiology"},{"issue":"3","key":"10.1016\/j.synbio.2026.02.009_bib37","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1002\/elps.200410070","article-title":"The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures","volume":"26","author":"Holmberg","year":"2005","journal-title":"Electrophoresis"},{"issue":"4","key":"10.1016\/j.synbio.2026.02.009_bib38","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S1050-3862(95)00160-3","article-title":"A biotinylated MutS fusion protein and its use in a rapid mutation screening technique","volume":"13","author":"Geschwind","year":"1996","journal-title":"Genet Anal Biomol Eng"},{"key":"10.1016\/j.synbio.2026.02.009_bib39","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jchromb.2017.12.024","article-title":"Aptamer facilitated purification of functional proteins","volume":"1073","author":"Beloborodov","year":"2018","journal-title":"J Chromatogr B"},{"issue":"12","key":"10.1016\/j.synbio.2026.02.009_bib40","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1021\/ar000033j","article-title":"Calculating structures and free energies of complex molecules: combining molecular mechanics and continuum models","volume":"33","author":"Kollman","year":"2000","journal-title":"Acc Chem Res"},{"issue":"3","key":"10.1016\/j.synbio.2026.02.009_bib41","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1093\/emboj\/cdg064","article-title":"The alternating ATPase domains of MutS control DNA mismatch repair","volume":"22","author":"Lamers","year":"2003","journal-title":"EMBO J"},{"issue":"4","key":"10.1016\/j.synbio.2026.02.009_bib42","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1002\/prot.22488","article-title":"Improved prediction of protein side-chain conformations with SCWRL4","volume":"77","author":"Krivov","year":"2009","journal-title":"Proteins"},{"issue":"2","key":"10.1016\/j.synbio.2026.02.009_bib43","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1021\/ct100578z","article-title":"PROPKA3: consistent treatment of internal and surface residues in empirical pKa predictions","volume":"7","author":"Olsson","year":"2011","journal-title":"J Chem Theor Comput"},{"issue":"2","key":"10.1016\/j.synbio.2026.02.009_bib44","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1063\/1.445869","article-title":"Comparison of simple potential functions for simulating liquid water","volume":"79","author":"Jorgensen","year":"1983","journal-title":"J Chem Phys"},{"issue":"18","key":"10.1016\/j.synbio.2026.02.009_bib45","doi-asserted-by":"crossref","first-page":"3586","DOI":"10.1021\/jp973084f","article-title":"All-atom empirical potential for molecular modeling and dynamics studies of proteins","volume":"102","author":"MacKerell","year":"1998","journal-title":"J Phys Chem B"},{"issue":"5","key":"10.1016\/j.synbio.2026.02.009_bib46","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1063\/1.1332996","article-title":"Langevin stabilization of molecular dynamics","volume":"114","author":"Izaguirre","year":"2001","journal-title":"J Chem Phys"},{"key":"10.1016\/j.synbio.2026.02.009_bib47","doi-asserted-by":"crossref","first-page":"3684","DOI":"10.1063\/1.448118","article-title":"Molecular-dynamics with coupling to an external Bath","volume":"81","author":"Hjcp","year":"1984","journal-title":"J Chem Phys"},{"issue":"12","key":"10.1016\/j.synbio.2026.02.009_bib48","doi-asserted-by":"crossref","first-page":"10089","DOI":"10.1063\/1.464397","article-title":"Particle mesh ewald: an Nlog(N) method for ewald sums in large systems","volume":"98","author":"Darden","year":"1993","journal-title":"J Chem Phys"},{"issue":"3","key":"10.1016\/j.synbio.2026.02.009_bib49","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/0021-9991(77)90098-5","article-title":"Numerical integration of the cartesian equations of motion of a system with constraints: molecular dynamics of n-alkanes","volume":"23","author":"Ryckaert","year":"1977","journal-title":"J Comput Phys"},{"issue":"9","key":"10.1016\/j.synbio.2026.02.009_bib50","doi-asserted-by":"crossref","first-page":"3314","DOI":"10.1021\/ct300418h","article-title":"MMPBSA.py: an efficient program for end-state free energy calculations","volume":"8","author":"Miller","year":"2012","journal-title":"J Chem Theor Comput"},{"issue":"2","key":"10.1016\/j.synbio.2026.02.009_bib51","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s10529-007-9543-y","article-title":"Construction of bifunctional fusion proteins consisting of duck BAFF and EGFP","volume":"30","author":"Dan","year":"2008","journal-title":"Biotechnol Lett"},{"issue":"4","key":"10.1016\/j.synbio.2026.02.009_bib52","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/j.fsigen.2011.09.003","article-title":"An optimized protocol for forensic application of the PreCRTM repair mix to multiplex STR amplification of UV-damaged DNA","volume":"6","author":"Diegoli","year":"2012","journal-title":"Forensic Sci Int Genet"},{"key":"10.1016\/j.synbio.2026.02.009_bib53","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2021.108096","article-title":"Protein engineering design from directed evolution to de novo synthesis","volume":"174","author":"Xiong","year":"2021","journal-title":"Biochem Eng J"},{"key":"10.1016\/j.synbio.2026.02.009_bib54","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2024.1504876","article-title":"Bacteriophage RNA polymerases: catalysts for mRNA vaccines and therapeutics","volume":"11","author":"Nair","year":"2024","journal-title":"Front Mol Biosci"},{"issue":"1","key":"10.1016\/j.synbio.2026.02.009_bib55","doi-asserted-by":"crossref","DOI":"10.1002\/biot.202200323","article-title":"Serial enrichment of heteroduplex DNA using a MutS-magnetic bead system","volume":"18","author":"Murphy","year":"2023","journal-title":"Biotechnol J"},{"issue":"1","key":"10.1016\/j.synbio.2026.02.009_bib56","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/s41587-021-00988-3","article-title":"Purification of multiplex oligonucleotide libraries by synthesis and selection","volume":"40","author":"Choi","year":"2022","journal-title":"Nat Biotechnol"},{"issue":"4","key":"10.1016\/j.synbio.2026.02.009_bib57","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1021\/acssynbio.6b00293","article-title":"Fully automated one-step synthesis of single-transcript TALEN pairs using a biological foundry","volume":"6","author":"Chao","year":"2017","journal-title":"ACS Synth Biol"},{"key":"10.1016\/j.synbio.2026.02.009_bib58","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/bs.mie.2018.04.011","article-title":"Multifragment DNA assembly of biochemical pathways via automated ligase cycling reaction","volume":"608","author":"Robinson","year":"2018","journal-title":"Methods Enzymol"},{"issue":"1","key":"10.1016\/j.synbio.2026.02.009_bib59","doi-asserted-by":"crossref","first-page":"2040","DOI":"10.1038\/s41467-019-10079-2","article-title":"Building a global alliance of biofoundries","volume":"10","author":"Hillson","year":"2019","journal-title":"Nat Commun"},{"issue":"11","key":"10.1016\/j.synbio.2026.02.009_bib60","first-page":"1249","article-title":"Engineering platforms for synthetic biology research","volume":"33","author":"Cui","year":"2018","journal-title":"Bull Chin Acad Sci"},{"issue":"3","key":"10.1016\/j.synbio.2026.02.009_bib61","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1360\/TB-2020-0498","article-title":"Automation in synthetic biology using biological foundries (in Chinese)","volume":"66","author":"Tang","year":"2021","journal-title":"Chin Sci Bull"},{"issue":"8-9","key":"10.1016\/j.synbio.2026.02.009_bib62","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1007\/s12033-016-9957-7","article-title":"T7 endonuclease I mediates error correction in artificial gene synthesis","volume":"58","author":"Sequeira","year":"2016","journal-title":"Mol Biotechnol"}],"container-title":["Synthetic and Systems Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405805X26000657?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405805X26000657?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:27:19Z","timestamp":1786994839000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405805X26000657"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":62,"alternative-id":["S2405805X26000657"],"URL":"https:\/\/doi.org\/10.1016\/j.synbio.2026.02.009","relation":{},"ISSN":["2405-805X"],"issn-type":[{"value":"2405-805X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Engineered eMutS based magnetic separation system for high-fidelity DNA synthesis","name":"articletitle","label":"Article Title"},{"value":"Synthetic and Systems Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.synbio.2026.02.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.","name":"copyright","label":"Copyright"}]}}