{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T19:24:56Z","timestamp":1783106696971,"version":"3.54.6"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T00:00:00Z","timestamp":1776556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFA0914200"],"award-info":[{"award-number":["2023YFA0914200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32360021"],"award-info":[{"award-number":["32360021"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Synthetic and Systems Biotechnology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.synbio.2026.04.019","type":"journal-article","created":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T05:37:05Z","timestamp":1778909825000},"page":"245-254","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Heterologous expression of hydroxylase coupled with pathway optimization enables highly efficient \u029f-DOPA production in Corynebacterium glutamicum"],"prefix":"10.1016","volume":"14","author":[{"given":"Mengzhen","family":"Jia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohe","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bangce","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0525-0474","authenticated-orcid":false,"given":"Bin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.synbio.2026.04.019_bib1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1080\/10826068.2021.1945624","article-title":"Kinetic modeling and statistical optimization of submerged production of anti-parkinson's prodrug L-DOPA by Pseudomonas fluorescens","volume":"52","author":"Naha","year":"2022","journal-title":"Prep Biochem Biotechnol"},{"key":"10.1016\/j.synbio.2026.04.019_bib2","doi-asserted-by":"crossref","first-page":"2284","DOI":"10.1016\/S0140-6736(21)00218-X","article-title":"Parkinson's disease","volume":"397","author":"Bloem","year":"2021","journal-title":"Lancet"},{"key":"10.1016\/j.synbio.2026.04.019_bib3","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1007\/s10295-019-02237-8","article-title":"Integrating enzyme evolution and high-throughput screening for efficient biosynthesis of L-DOPA","volume":"46","author":"Zeng","year":"2019","journal-title":"J Ind Microbiol Biotechnol"},{"key":"10.1016\/j.synbio.2026.04.019_bib4","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1038\/nrn2471","article-title":"Molecular mechanisms of L-DOPA-induced dyskinesia","volume":"9","author":"Jenner","year":"2008","journal-title":"Nat Rev Neurosci"},{"key":"10.1016\/j.synbio.2026.04.019_bib5","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1002\/mds.26120","article-title":"Four pioneers of L-dopa treatment: Arvid Carlsson, Oleh Hornykiewicz, George Cotzias, and Melvin Yahr","volume":"30","author":"Lees","year":"2015","journal-title":"Mov Disord"},{"key":"10.1016\/j.synbio.2026.04.019_bib6","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/S0140-6736(77)91940-7","article-title":"Implications of combined treatment with 'Madopar' and L-deprenil in Parkinson's disease. A long-term study","volume":"1","author":"Birkmayer","year":"1977","journal-title":"Lancet"},{"key":"10.1016\/j.synbio.2026.04.019_bib7","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/S0304-3940(02)01465-9","article-title":"Functional MRI of amblyopia before and after levodopa","volume":"339","author":"Yang","year":"2003","journal-title":"Neurosci Lett"},{"key":"10.1016\/j.synbio.2026.04.019_bib8","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1007\/s00213-003-1672-y","article-title":"A meta-analysis of the response to chronic L-dopa in patients with schizophrenia: therapeutic and heuristic implications","volume":"171","author":"Jaskiw","year":"2004","journal-title":"Psychopharmacology (Berl)"},{"key":"10.1016\/j.synbio.2026.04.019_bib9","doi-asserted-by":"crossref","first-page":"9103","DOI":"10.1007\/s00253-021-11681-5","article-title":"Tyrosinase-based production of L-DOPA by Corynebacterium glutamicum","volume":"105","author":"Kurpejovi\u0107","year":"2021","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.synbio.2026.04.019_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.jinorgbio.2022.111878","article-title":"Biomimetic synthesis of L-DOPA inspired by tyrosine hydroxylase","volume":"234","author":"Du","year":"2022","journal-title":"J Inorg Biochem"},{"key":"10.1016\/j.synbio.2026.04.019_bib11","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1007\/s00284-008-9287-5","article-title":"Transformation of L-tyrosine to L-dopa by a novel fungus, Acremonium rutilum, under submerged fermentation","volume":"58","author":"Krishnaveni","year":"2009","journal-title":"Curr Microbiol"},{"key":"10.1016\/j.synbio.2026.04.019_bib12","doi-asserted-by":"crossref","first-page":"81","DOI":"10.4236\/apd.2013.23016","article-title":"Biological sources of L-DOPA: an alternative approach","volume":"2","author":"Patil S","year":"2013","journal-title":"Adv Parkinson's Dis"},{"key":"10.1016\/j.synbio.2026.04.019_bib13","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1076\/1388-0209(200009)38:4;1-A;FT271","article-title":"In vitro production of L-dopa in tissue cultures of banana","volume":"38","author":"Bapat","year":"2000","journal-title":"Pharm Biol"},{"key":"10.1016\/j.synbio.2026.04.019_bib14","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1021\/ed063p222","article-title":"Application of organometallic catalysis to the commercial production of L-DOPA","volume":"63","author":"Knowles","year":"1986","journal-title":"J Chem Educ"},{"key":"10.1016\/j.synbio.2026.04.019_bib15","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.catcom.2004.07.018","article-title":"Production of L-DOPA under heterogeneous asymmetric catalysis","volume":"5","author":"Vald\u00e9s","year":"2004","journal-title":"Catal Commun"},{"key":"10.1016\/j.synbio.2026.04.019_bib16","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s12934-019-1122-0","article-title":"Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of L-DOPA from D-glucose","volume":"18","author":"Fordjour","year":"2019","journal-title":"Microb Cell Fact"},{"key":"10.1016\/j.synbio.2026.04.019_bib17","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1007\/s10295-011-0973-0","article-title":"Metabolic engineering of Escherichia coli for improving L-3,4-dihydroxyphenylalanine (L-DOPA) synthesis from glucose","volume":"38","author":"Mu\u00f1oz","year":"2011","journal-title":"J Ind Microbiol Biotechnol"},{"key":"10.1016\/j.synbio.2026.04.019_bib18","article-title":"Genome engineering Escherichia coli for L-DOPA overproduction from glucose","volume":"6","author":"Wei","year":"2016","journal-title":"Sci Rep"},{"key":"10.1016\/j.synbio.2026.04.019_bib19","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1080\/10826068.2018.1487851","article-title":"Metabolic engineering of Escherichia coli W3110 strain by incorporating genome-level modifications and synthetic plasmid modules to enhance L-Dopa production from glycerol","volume":"48","author":"Das","year":"2018","journal-title":"Prep Biochem Biotechnol"},{"key":"10.1016\/j.synbio.2026.04.019_bib20","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1021\/acssynbio.1c00411","article-title":"Combining precursor-directed engineering with modular designing: an effective strategy for de novo biosynthesis of L-DOPA in Bacillus licheniformis","volume":"11","author":"Xu","year":"2022","journal-title":"ACS Synth Biol"},{"key":"10.1016\/j.synbio.2026.04.019_bib21","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1590\/S1517-83822006000100015","article-title":"Mutation of Aspergillus oryzae for improved production of 3, 4-dihydroxy Phenyl-L-Alanine (L-Dopa) from L-tyrosine","volume":"37","author":"Haq","year":"2006","journal-title":"Braz J Microbiol"},{"key":"10.1016\/j.synbio.2026.04.019_bib22","doi-asserted-by":"crossref","first-page":"440","DOI":"10.3389\/fbioe.2019.00440","article-title":"Recent advances of L-ornithine biosynthesis in metabolically engineered Corynebacterium glutamicum","volume":"7","author":"Wu","year":"2019","journal-title":"Front Bioeng Biotechnol"},{"key":"10.1016\/j.synbio.2026.04.019_bib23","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.biortech.2019.03.122","article-title":"Metabolic engineering of Corynebacterium glutamicum S9114 to enhance the production of l-ornithine driven by glucose and xylose","volume":"284","author":"Zhang","year":"2019","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.synbio.2026.04.019_bib24","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0168-1656(03)00154-8","article-title":"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins","volume":"104","author":"Kalinowski","year":"2003","journal-title":"J Biotechnol"},{"key":"10.1016\/j.synbio.2026.04.019_bib25","doi-asserted-by":"crossref","first-page":"3237","DOI":"10.1021\/acssynbio.1c00355","article-title":"Synthetic biology toolkits and metabolic engineering applied in Corynebacterium glutamicum for biomanufacturing","volume":"10","author":"Chai","year":"2021","journal-title":"ACS Synth Biol"},{"key":"10.1016\/j.synbio.2026.04.019_bib26","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.copbio.2020.06.006","article-title":"Recent advances in improving metabolic robustness of microbial cell factories","volume":"66","author":"Jiang","year":"2020","journal-title":"Curr Opin Biotechnol"},{"key":"10.1016\/j.synbio.2026.04.019_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2025.108741","article-title":"Accelerated adaptive laboratory evolution: a tool for evolutionary biotechnology","volume":"86","author":"Declerck","year":"2026","journal-title":"Biotechnol Adv"},{"key":"10.1016\/j.synbio.2026.04.019_bib28","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/s13205-018-1267-2","article-title":"Pathway engineering in Corynebacterium glutamicum S9114 for 5-aminolevulinic acid production","volume":"8","author":"Zhang","year":"2018","journal-title":"3 Biotech"},{"key":"10.1016\/j.synbio.2026.04.019_bib29","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s12934-019-1122-0","article-title":"Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of l-DOPA from d-glucose","volume":"18","author":"Fordjour","year":"2019","journal-title":"Microb Cell Fact"},{"key":"10.1016\/j.synbio.2026.04.019_bib30","doi-asserted-by":"crossref","first-page":"7224","DOI":"10.1128\/AEM.71.11.7224-7228.2005","article-title":"Feedback inhibition of chorismate mutase\/prephenate dehydrogenase (TyrA) of Escherichia coli: generation and characterization of tyrosine-insensitive mutants","volume":"71","author":"L\u00fctke-Eversloh","year":"2005","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.synbio.2026.04.019_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2025.132879","article-title":"Reprogramming Corynebacterium glutamicum metabolism to efficiently synthesis protocatechuic acid from flask to pilot scale","volume":"435","author":"Shi","year":"2025","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.synbio.2026.04.019_bib32","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.ymben.2016.08.005","article-title":"Metabolic engineering of Corynebacterium glutamicum for shikimate overproduction by growth-arrested cell reaction","volume":"38","author":"Kogure","year":"2016","journal-title":"Metab Eng"},{"key":"10.1016\/j.synbio.2026.04.019_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2022.108073","article-title":"Shikimic acid biosynthesis in microorganisms: current status and future direction","volume":"62","author":"Sheng","year":"2023","journal-title":"Biotechnol Adv"}],"container-title":["Synthetic and Systems Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405805X26001110?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405805X26001110?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T19:14:09Z","timestamp":1783106049000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405805X26001110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":33,"alternative-id":["S2405805X26001110"],"URL":"https:\/\/doi.org\/10.1016\/j.synbio.2026.04.019","relation":{},"ISSN":["2405-805X"],"issn-type":[{"value":"2405-805X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Heterologous expression of hydroxylase coupled with pathway optimization enables highly efficient \u029f-DOPA production in Corynebacterium glutamicum","name":"articletitle","label":"Article Title"},{"value":"Synthetic and Systems Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.synbio.2026.04.019","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.","name":"copyright","label":"Copyright"}]}}