{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:59:57Z","timestamp":1790200797276,"version":"4.1.0"},"reference-count":24,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,4,1]],"date-time":"2027-04-01T00:00:00Z","timestamp":1806537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,4,1]],"date-time":"2027-04-01T00:00:00Z","timestamp":1806537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32500053"],"award-info":[{"award-number":["32500053"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People&apos;s Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC3402300"],"award-info":[{"award-number":["2023YFC3402300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Synthetic and Systems Biotechnology"],"published-print":{"date-parts":[[2027,4]]},"DOI":"10.1016\/j.synbio.2026.09.002","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T09:12:01Z","timestamp":1789722721000},"page":"142-149","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Hijacking host RNA polymerase for processive in situ mutagenesis"],"prefix":"10.1016","volume":"18","author":[{"given":"Yuou","family":"Sheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huizhen","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shihao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenliang","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.synbio.2026.09.002_bib1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/s43586-022-00119-5","article-title":"In vivo hypermutation and continuous evolution","volume":"2","author":"Molina","year":"2022","journal-title":"Nat Rev Methods Primers"},{"issue":"20","key":"10.1016\/j.synbio.2026.09.002_bib2","doi-asserted-by":"crossref","first-page":"12384","DOI":"10.1021\/acs.chemrev.1c00260","article-title":"Directed evolution: methodologies and applications","volume":"121","author":"Wang","year":"2021","journal-title":"Chem Rev"},{"issue":"7","key":"10.1016\/j.synbio.2026.09.002_bib3","doi-asserted-by":"crossref","first-page":"1946","DOI":"10.1016\/j.cell.2018.10.021","article-title":"Scalable, continuous evolution of genes at mutation rates above genomic error thresholds","volume":"175","author":"Ravikumar","year":"2018","journal-title":"Cell"},{"issue":"7717","key":"10.1016\/j.synbio.2026.09.002_bib4","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/s41586-018-0384-8","article-title":"CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window","volume":"560","author":"Halperin","year":"2018","journal-title":"Nature"},{"issue":"6","key":"10.1016\/j.synbio.2026.09.002_bib5","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.1016\/j.tibtech.2025.02.011","article-title":"OMEGA-guided DNA polymerases enable random mutagenesis in a tunable window","volume":"43","author":"Liu","year":"2025","journal-title":"Trends Biotechnol"},{"issue":"45","key":"10.1016\/j.synbio.2026.09.002_bib6","article-title":"CRISPR-DNA polymerase assisted targeted mutagenesis for regulable laboratory evolution","volume":"12","author":"Chen","year":"2025","journal-title":"Adv Sci (Weinh)"},{"issue":"1","key":"10.1016\/j.synbio.2026.09.002_bib7","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1038\/s41467-023-39087-z","article-title":"A Cas3-base editing tool for targetable in vivo mutagenesis","volume":"14","author":"Zimmermann","year":"2023","journal-title":"Nat Commun"},{"issue":"37","key":"10.1016\/j.synbio.2026.09.002_bib8","doi-asserted-by":"crossref","first-page":"11560","DOI":"10.1021\/jacs.8b04001","article-title":"A processive protein chimera introduces mutations across defined DNA regions in vivo","volume":"140","author":"Moore","year":"2018","journal-title":"J Am Chem Soc"},{"issue":"1","key":"10.1016\/j.synbio.2026.09.002_bib9","doi-asserted-by":"crossref","first-page":"6041","DOI":"10.1038\/s41467-025-61354-4","article-title":"An orthogonal transcription mutation system generating all transition mutations for accelerated protein evolution in vivo","volume":"16","author":"Shao","year":"2025","journal-title":"Nat Commun"},{"issue":"10","key":"10.1016\/j.synbio.2026.09.002_bib10","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.tim.2006.08.002","article-title":"The evolving story of the omega subunit of bacterial RNA polymerase","volume":"14","author":"Mathew","year":"2006","journal-title":"Trends Microbiol"},{"issue":"7","key":"10.1016\/j.synbio.2026.09.002_bib11","doi-asserted-by":"crossref","first-page":"4606","DOI":"10.1093\/nar\/gku084","article-title":"The Omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria","volume":"42","author":"Gunnelius","year":"2014","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"10.1016\/j.synbio.2026.09.002_bib13","doi-asserted-by":"crossref","first-page":"5909","DOI":"10.1038\/s41467-024-50243-x","article-title":"TracrRNA reprogramming enables direct PAM-independent detection of RNA with diverse DNA-targeting Cas12 nucleases","volume":"15","author":"Jiao","year":"2024","journal-title":"Nat Commun"},{"issue":"1","key":"10.1016\/j.synbio.2026.09.002_bib14","doi-asserted-by":"crossref","first-page":"6191","DOI":"10.1038\/s41467-021-26469-4","article-title":"Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells","volume":"12","author":"Bigelyte","year":"2021","journal-title":"Nat Commun"},{"issue":"6501","key":"10.1016\/j.synbio.2026.09.002_bib15","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1126\/science.abb1400","article-title":"CRISPR-Cas\u03a6 from huge phages is a hypercompact genome editor","volume":"369","author":"Pausch","year":"2020","journal-title":"Science"},{"issue":"6448","key":"10.1016\/j.synbio.2026.09.002_bib16","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1126\/science.aax9181","article-title":"RNA-guided DNA insertion with CRISPR-associated transposases","volume":"365","author":"Strecker","year":"2019","journal-title":"Science"},{"issue":"21","key":"10.1016\/j.synbio.2026.09.002_bib17","doi-asserted-by":"crossref","first-page":"4457","DOI":"10.1016\/j.molcel.2021.07.043","article-title":"Structural basis of target DNA recognition by CRISPR-Cas12k for RNA-guided DNA transposition","volume":"81","author":"Xiao","year":"2021","journal-title":"Mol Cell"},{"issue":"5","key":"10.1016\/j.synbio.2026.09.002_bib18","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1016\/j.cell.2013.02.022","article-title":"Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression","volume":"152","author":"Qi","year":"2013","journal-title":"Cell"},{"issue":"1","key":"10.1016\/j.synbio.2026.09.002_bib19","doi-asserted-by":"crossref","first-page":"7199","DOI":"10.1038\/s41598-017-07314-5","article-title":"Uracil accumulation and mutagenesis dominated by cytosine deamination in CpG dinucleotides in mice lacking UNG and SMUG1","volume":"7","author":"Als\u00f8e","year":"2017","journal-title":"Sci Rep"},{"issue":"2","key":"10.1016\/j.synbio.2026.09.002_bib20","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.molcel.2019.04.029","article-title":"Structural basis of transcription: RNA polymerase backtracking and its reactivation","volume":"75","author":"Abdelkareem","year":"2019","journal-title":"Mol Cell"},{"issue":"1","key":"10.1016\/j.synbio.2026.09.002_bib21","doi-asserted-by":"crossref","first-page":"3193","DOI":"10.1038\/s41467-024-47531-x","article-title":"Reciprocating RNA Polymerase batters through roadblocks","volume":"15","author":"Qian","year":"2024","journal-title":"Nat Commun"},{"issue":"3","key":"10.1016\/j.synbio.2026.09.002_bib22","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/S0092-8674(03)00600-7","article-title":"Structure and function of the transcription elongation factor GreB bound to bacterial RNA polymerase","volume":"114","author":"Opalka","year":"2003","journal-title":"Cell"},{"issue":"7603","key":"10.1016\/j.synbio.2026.09.002_bib23","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/nature17946","article-title":"Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage","volume":"533","author":"Komor","year":"2016","journal-title":"Nature"},{"issue":"11","key":"10.1016\/j.synbio.2026.09.002_bib24","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1089\/cmb.2017.0096","article-title":"cutPrimers: a new tool for accurate cutting of primers from reads of targeted next generation sequencing","volume":"24","author":"Kechin","year":"2017","journal-title":"J Comput Biol"},{"key":"10.1016\/j.synbio.2026.09.002_bib25","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/1471-2105-13-31","article-title":"PANDAseq: paired-end assembler for illumina sequences","volume":"13","author":"Masella","year":"2012","journal-title":"BMC Bioinf"}],"container-title":["Synthetic and Systems Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405805X26002127?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405805X26002127?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:03:15Z","timestamp":1790197395000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405805X26002127"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,4]]},"references-count":24,"alternative-id":["S2405805X26002127"],"URL":"https:\/\/doi.org\/10.1016\/j.synbio.2026.09.002","relation":{},"ISSN":["2405-805X"],"issn-type":[{"value":"2405-805X","type":"print"}],"subject":[],"published":{"date-parts":[[2027,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Hijacking host RNA polymerase for processive in situ mutagenesis","name":"articletitle","label":"Article Title"},{"value":"Synthetic and Systems Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.synbio.2026.09.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.","name":"copyright","label":"Copyright"}]}}