{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T17:49:13Z","timestamp":1772646553311,"version":"3.50.1"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"National Council of Science and Technology-Mexico","doi-asserted-by":"crossref","award":["#CB-284183"],"award-info":[{"award-number":["#CB-284183"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Council of Science and Technology"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicology and Applied Pharmacology"],"published-print":{"date-parts":[[2019,10]]},"DOI":"10.1016\/j.taap.2019.114694","type":"journal-article","created":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T23:41:15Z","timestamp":1564184475000},"page":"114694","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Low concentrations of lead decrease the sperm fertilization ability by altering the acrosome reaction in mice"],"prefix":"10.1016","volume":"380","author":[{"given":"Yazm\u00edn","family":"God\u00ednez-Sol\u00eds","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda de Jes\u00faa","family":"Sol\u00eds-Heredia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Roa-Espitia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lyda Yuliana","family":"Parra-Forero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrique O.","family":"Hern\u00e1ndez-Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4427-6282","authenticated-orcid":false,"given":"Isabel","family":"Hern\u00e1ndez-Ochoa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8065-6840","authenticated-orcid":false,"given":"Betzabet","family":"Quintanilla-Vega","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.taap.2019.114694_bb0005","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1006\/abbi.2000.1880","article-title":"Mammalian sperm acrosome: formation, contents, and function","volume":"379","author":"Abou-Haila","year":"2000","journal-title":"Arch. Biochem. Biophys."},{"issue":"3","key":"10.1016\/j.taap.2019.114694_bb0010","doi-asserted-by":"crossref","first-page":"291","DOI":"10.2486\/indhealth.41.291","article-title":"Lead acetate induced cytotoxicity in male germinal cells of Swiss mice","volume":"41","author":"Acharya","year":"2008","journal-title":"Ind. Health"},{"issue":"2","key":"10.1016\/j.taap.2019.114694_bb0015","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1002\/j.1939-4640.1993.tb01667.x","article-title":"Analysis of the responses of human spermatozoa to A23187 employing a novel technique for assessing the acrosome reaction","volume":"14","author":"Aitken","year":"1993","journal-title":"J. Androl."},{"issue":"Pt 1","key":"10.1016\/j.taap.2019.114694_bb0020","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1042\/bj3540149","article-title":"LIM-kinase 2 induces formation of stress fibres, focal adhesions and membrane blebs, dependent on its activation by rho-associated kinase-catalysed phosphorylation at threonine-505","volume":"354","author":"Amano","year":"2001","journal-title":"The Biochem. J."},{"issue":"3","key":"10.1016\/j.taap.2019.114694_bb0025","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1002\/1097-0274(200009)38:3<310::AID-AJIM10>3.0.CO;2-9","article-title":"The effect of lead on male fertility: a time to pregnancy (TTP) study","volume":"38","author":"Apostoli","year":"2000","journal-title":"Am. J. Ind. Med."},{"issue":"2","key":"10.1016\/j.taap.2019.114694_bb0030","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1093\/humrep\/deg020","article-title":"Increased seminal plasma lead levels adversely affect the fertility potential of sperm in IVF","volume":"18","author":"Benoff","year":"2003","journal-title":"Hum. Reprod."},{"issue":"1\u20132","key":"10.1016\/j.taap.2019.114694_bb0035","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S0165-0378(01)00085-7","article-title":"Role and regulation of intracellular calcium in acrosomal exocytosis","volume":"53","author":"Breitbart","year":"2002","journal-title":"J. Reprod. Immunol."},{"issue":"3","key":"10.1016\/j.taap.2019.114694_bb0040","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1093\/molehr\/3.3.195","article-title":"The biochemistry of the acrosome reaction","volume":"3","author":"Breitbart","year":"1997","journal-title":"Mol. Hum. Reprod."},{"issue":"3","key":"10.1016\/j.taap.2019.114694_bb0045","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1095\/biolreprod.102.009233","article-title":"Remodeling of the actin cytoskeleton during mammalian sperm capacitation and acrosome reaction1","volume":"68","author":"Brener","year":"2004","journal-title":"Biol. Reprod."},{"issue":"3","key":"10.1016\/j.taap.2019.114694_bb0050","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1002\/mrd.1120150302","article-title":"Development of ability to penetrate the cumulus oophorus by hamster spermatozoa capacitated in vitro, in relation to the timing of the acrosome reaction","volume":"15","author":"Cummins","year":"1986","journal-title":"Gamete Res."},{"key":"10.1016\/j.taap.2019.114694_bb0055","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0074-7696(05)43002-8","article-title":"Calcium channels and Ca2+ fluctuations in sperm physiology","volume":"243","author":"Darszon","year":"2005","journal-title":"Int. Rev. Cytol."},{"issue":"1","key":"10.1016\/j.taap.2019.114694_bb0060","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/j.1365-2605.1993.tb01148.x","article-title":"A positive role for the superoxide anion in triggering hyperactivation and capacitation of human spermatozoa","volume":"16","author":"De Lamirande","year":"1993","journal-title":"Int. J. Androl."},{"issue":"2","key":"10.1016\/j.taap.2019.114694_bb0065","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1002\/mrd.20192","article-title":"Actin polymerization in the equatorial and postacrosomal regions of guinea pig spermatozoa during the acrosome reaction is regulated by G proteins","volume":"70","author":"Delgado-Buenrostro","year":"2005","journal-title":"Mol. Reprod. Dev."},{"issue":"03","key":"10.1016\/j.taap.2019.114694_bb0070","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1017\/S0967199406003790","article-title":"Localization of the Rho GTPases and some rho effector proteins in the sperm of several mammalian species","volume":"14","author":"Ducummon","year":"2006","journal-title":"Zygote"},{"issue":"4","key":"10.1016\/j.taap.2019.114694_bb0075","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1534\/genetics.110.126094","article-title":"Functional relevance of CpG Island length for regulation of gene expression","volume":"187","author":"Elango","year":"2011","journal-title":"Genetics"},{"issue":"2","key":"10.1016\/j.taap.2019.114694_bb0080","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1093\/toxsci\/kfr352","article-title":"Differing effects of toxicants (methylmercury, inorganic mercury, lead, amyloid \u03b2, and rotenone) on cultured rat cerebrocortical neurons: differential expression of rho proteins associated with neurotoxicity","volume":"126","author":"Fujimura","year":"2012","journal-title":"Toxicol. Sci."},{"issue":"4","key":"10.1016\/j.taap.2019.114694_bb0085","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1620\/tjem.238.295","article-title":"Lead inhibits human sperm functions by reducing the levels of intracellular calcium, cAMP, and tyrosine phosphorylation","volume":"238","author":"He","year":"2016","journal-title":"Tohoku J. Exp. Med."},{"issue":"2","key":"10.1016\/j.taap.2019.114694_bb0090","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.reprotox.2005.01.007","article-title":"Low lead environmental exposure alters semen quality and sperm chromatin condensation in Northern Mexico","volume":"20","author":"Hern\u00e1ndez-Ochoa","year":"2005","journal-title":"Reprod. Toxicol."},{"issue":"3","key":"10.1016\/j.taap.2019.114694_bb0095","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1083\/jcb.148.3.481","article-title":"Cdc42 and Rac stimulate exocytosis of secretory granules by activating the IP(3)\/calcium pathway in RBL-2H3 mast cells","volume":"148","author":"Hong-Geller","year":"2000","journal-title":"J. Cell Biol."},{"issue":"1","key":"10.1016\/j.taap.2019.114694_bb0100","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1002\/j.1939-4640.2001.tb02154.x","article-title":"Actin and actin-binding proteins in bovine spermatozoa: potential role in membrane remodeling and intracellular signaling during epididymal maduration and the acrosome reaction","volume":"22","author":"Howes","year":"2001","journal-title":"J. Androl."},{"issue":"1\u20132","key":"10.1016\/j.taap.2019.114694_bb0105","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0300-483X(97)00090-5","article-title":"Lead exposure causes generation of reactive oxygen species and functional impairment in rat sperm","volume":"122","author":"Hsu","year":"1997","journal-title":"Toxicology"},{"issue":"2","key":"10.1016\/j.taap.2019.114694_bb0110","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/0300-483X(89)90041-3","article-title":"Premature acrosome reaction in spermatozoa from lead-exposed mice","volume":"54","author":"Johansson","year":"1989","journal-title":"Toxicology"},{"issue":"2","key":"10.1016\/j.taap.2019.114694_bb0115","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/S0013-9351(86)80142-6","article-title":"Long-term exposure of the male mouse to lead: effects on fertility","volume":"41","author":"Johansson","year":"1986","journal-title":"Environ. Res."},{"issue":"2","key":"10.1016\/j.taap.2019.114694_bb0120","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1113\/jphysiol.2003.039073","article-title":"Facilitation of Ca 2+ \u2212dependent exocytosis by Rac1-GTPase in bovine chromaffin cells","volume":"550","author":"Li","year":"2003","journal-title":"J. Physiol."},{"issue":"5","key":"10.1016\/j.taap.2019.114694_bb0125","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1289\/ehp.1408577","article-title":"Lead exposure during early human development and DNA methylation of imprinted gene regulatory elements in adulthood","volume":"124","author":"Li","year":"2016","journal-title":"Environ. Health Perspect."},{"issue":"11","key":"10.1016\/j.taap.2019.114694_bb0130","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1097\/00043764-200011000-00007","article-title":"Lactation and lead body burden turnover: a pilot study in Mexico","volume":"42","author":"Moline","year":"2000","journal-title":"J. Occup. Environ. Med."},{"issue":"Pt 6","key":"10.1016\/j.taap.2019.114694_bb0135","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1242\/jcs.038109","article-title":"Betapix-activated Rac1 stimulates the activation of phospholipase D, which is associated with exocytosis in neuroendocrine cells","volume":"122","author":"Momboisse","year":"2009","journal-title":"J. Cell Sci."},{"issue":"4","key":"10.1016\/j.taap.2019.114694_bb0140","first-page":"267","article-title":"Chronic environmental exposure to lead affects semen quality in a Mexican men population","volume":"11","author":"Mor\u00e1n-Mart\u00ednez","year":"2013","journal-title":"Iran. J. Reprod. Med."},{"issue":"1","key":"10.1016\/j.taap.2019.114694_bb0145","doi-asserted-by":"crossref","first-page":"109","DOI":"10.2119\/molmed.2014.00158","article-title":"Investigation on the origin of sper DNA fragmentation: role of apoptosis, immaturity and oxidative stress","volume":"21","author":"Muratori","year":"2015","journal-title":"Mol. Med."},{"issue":"5","key":"10.1016\/j.taap.2019.114694_bb0150","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/0890-6238(95)00040-H","article-title":"Nuclear alterations during acrosomal cap formation in spermatids of lead-treated rats","volume":"9","author":"Murthy","year":"1995","journal-title":"Reprod. Toxicol."},{"issue":"3","key":"10.1016\/j.taap.2019.114694_bb0155","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.tiv.2015.01.002","article-title":"In vitro lead exposure changes DNA methylation and expression of IGF2 and PEG1\/MEST","volume":"29","author":"Nye","year":"2015","journal-title":"Toxicol. in Vitro"},{"issue":"4","key":"10.1016\/j.taap.2019.114694_bb0160","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s10565-008-9088-4","article-title":"Lead chloride affects sperm motility and acrosome reaction in mice : llead affects mice sperm motility and acrosome reaction","volume":"25","author":"Oliveira","year":"2009","journal-title":"Cell Biol. Toxicol."},{"issue":"1","key":"10.1016\/j.taap.2019.114694_bb0165","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.semcdb.2010.12.002","article-title":"Rho GTPases and exocytosis: what are the molecular links?","volume":"22","author":"Ory","year":"2011","journal-title":"Semin. Cell Dev. Biol."},{"issue":"9","key":"10.1016\/j.taap.2019.114694_bb0170","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1289\/ehp.0800497","article-title":"Influence of prenatal lead exposure on genomic methylation of cord blood DNA","volume":"117","author":"Pilsner","year":"2009","journal-title":"Environ. Health Perspect."},{"issue":"2","key":"10.1016\/j.taap.2019.114694_bb0175","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.taap.2009.05.008","article-title":"Methyl-parathion decreases sperm function and fertilization capacity after targeting spermatocytes and maturing spermatozoa","volume":"238","author":"Pi\u00f1a-Guzm\u00e1n","year":"2009","journal-title":"Toxicol. Appl. Pharmacol."},{"issue":"3","key":"10.1016\/j.taap.2019.114694_bb0180","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/0009-2797(95)03646-6","article-title":"Lead-binding proteins in brain tissue of environmentally lead-exposed humans","volume":"98","author":"Quintanilla-Vega","year":"1995","journal-title":"Chem. Biol. Interact."},{"issue":"7","key":"10.1016\/j.taap.2019.114694_bb0185","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1021\/tx000017v","article-title":"Lead interaction with human protamine (HP2) as a mechanism of male reproductive toxicity","volume":"13","author":"Quintanilla-Vega","year":"2000","journal-title":"Chem. Res. Toxicol."},{"issue":"3","key":"10.1016\/j.taap.2019.114694_bb0190","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1002\/glia.22280","article-title":"Astrocyte stellation, a process dependent on Rac1 is sustained by the regulated exocytosis of enlargeosomes","volume":"60","author":"Racchetti","year":"2012","journal-title":"Glia"},{"issue":"2","key":"10.1016\/j.taap.2019.114694_bb0195","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.ydbio.2015.07.008","article-title":"PKA-dependent phosphorylation of LIMK1 and cofilin is essential for mouse sperm acrosomal exocytosis","volume":"405","author":"Romarowski","year":"2016","journal-title":"Dev. Biol."},{"issue":"1","key":"10.1016\/j.taap.2019.114694_bb0200","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S0009-2797(98)00060-X","article-title":"High-affinity renal lead-binding proteins in environmentally-exposed humans","volume":"115","author":"Smith","year":"1998","journal-title":"Chem. Biol. Interact."},{"issue":"10","key":"10.1016\/j.taap.2019.114694_bb0205","doi-asserted-by":"crossref","first-page":"R409","DOI":"10.1016\/S0960-9822(03)00324-5","article-title":"Control of vesicular trafficking by rho GTPases","volume":"13","author":"Symons","year":"2003","journal-title":"Curr. Biol."},{"issue":"1","key":"10.1016\/j.taap.2019.114694_bb0210","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1289\/ehp.0010845","article-title":"Semen quality and reproductive endocrine function in relation to biomarkers of lead, cadmium, zinc, and copper in men","volume":"108","author":"Telisman","year":"2000","journal-title":"Environ. Health Perspect."},{"issue":"4","key":"10.1016\/j.taap.2019.114694_bb0215","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1242\/dev.121.4.1139","article-title":"Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway","volume":"121","author":"Visconti","year":"1995","journal-title":"Dev. (Camb. England)."},{"issue":"12","key":"10.1016\/j.taap.2019.114694_bb0220","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1002\/jat.2795","article-title":"Maternal lead exposure during lactation persistently impairs testicular development and steroidogenesis in male offspring","volume":"33","author":"Wang","year":"2013","journal-title":"J. Appl. Toxicol."},{"key":"10.1016\/j.taap.2019.114694_bb0225","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.reprotox.2013.10.003","article-title":"Subchronic exposure to lead acetate inhibits spermatogenesis and downregulates the expression of Ddx3y in testis of mice","volume":"42","author":"Wang","year":"2013","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.taap.2019.114694_bb0230","series-title":"World Health Organization. Laboratory Manual for the Examination and Processing of Human Semen","author":"WHO","year":"2010"},{"key":"10.1016\/j.taap.2019.114694_bb0235","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/978-1-4684-4016-4_6","article-title":"Mechanisms of fertilization in mammals","author":"Yanagimachi","year":"1981","journal-title":"Fertilization Embryonic Dev. Vitro."}],"container-title":["Toxicology and Applied Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041008X19303023?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041008X19303023?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,7,21]],"date-time":"2024-07-21T11:58:36Z","timestamp":1721563116000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041008X19303023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":47,"alternative-id":["S0041008X19303023"],"URL":"https:\/\/doi.org\/10.1016\/j.taap.2019.114694","relation":{},"ISSN":["0041-008X"],"issn-type":[{"value":"0041-008X","type":"print"}],"subject":[],"published":{"date-parts":[[2019,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Low concentrations of lead decrease the sperm fertilization ability by altering the acrosome reaction in mice","name":"articletitle","label":"Article Title"},{"value":"Toxicology and Applied Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.taap.2019.114694","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"114694"}}