{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:21:13Z","timestamp":1788236473676,"version":"build-2803163510"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100007560","name":"Chiesi Farmaceutici","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007560","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003500","name":"Universit\u00e0 degli Studi di Padova","doi-asserted-by":"publisher","award":["ARD-B 2025"],"award-info":[{"award-number":["ARD-B 2025"]}],"id":[{"id":"10.13039\/501100003500","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicology and Applied Pharmacology"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.taap.2026.117853","type":"journal-article","created":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T15:00:49Z","timestamp":1777993249000},"page":"117853","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["An innovative label-free approach for investigating epithelial-mesenchymal transition: pharmacological characterization of TGF-\u03b21 effects in A549 cells"],"prefix":"10.1016","volume":"513","author":[{"given":"Francine Medjiofack","family":"Djeujo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Solimano","family":"Negrini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Ferri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Mion","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Ruscitti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Pitozzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Malfacini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello","family":"Trevisani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gessica","family":"Marchini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Girolamo","family":"Cal\u00f2","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.taap.2026.117853_bb0005","doi-asserted-by":"crossref","first-page":"2048","DOI":"10.1038\/s41598-021-81735-1","article-title":"Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT","volume":"11","author":"Aban","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.taap.2026.117853_bb0010","first-page":"332","article-title":"Intedanib, a triple kinase inhibitor of VEGFR, FGFR, and PDGFR, for the treatment of cancer and idiopathic pulmonary fibrosis","volume":"13","author":"Antoniu","year":"2010","journal-title":"IDrugs"},{"key":"10.1016\/j.taap.2026.117853_bb0015","doi-asserted-by":"crossref","first-page":"832","DOI":"10.3390\/mi11090832","article-title":"Electrical impedance spectroscopy for monitoring chemoresistance of cancer cells","volume":"11","author":"Crowell","year":"2020","journal-title":"Micromachines (Basel)"},{"key":"10.1016\/j.taap.2026.117853_bb0020","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1042\/BSR20140031","article-title":"Using real-time impedance-based assays to monitor the effects of fibroblast-derived media on the adhesion, proliferation, migration and invasion of colon cancer cells","volume":"34","author":"Dowling","year":"2014","journal-title":"Biosci. Rep."},{"key":"10.1016\/j.taap.2026.117853_bb0025","doi-asserted-by":"crossref","first-page":"2740","DOI":"10.3390\/cells12232740","article-title":"A new era of integration between multiomics and Spatio-temporal analysis for the translation of EMT towards clinical applications in Cancer","volume":"12","author":"Fonseca Teixeira","year":"2023","journal-title":"Cells"},{"key":"10.1016\/j.taap.2026.117853_bb0030","doi-asserted-by":"crossref","first-page":"7667","DOI":"10.1038\/s41598-019-43409-x","article-title":"TGF\u03b2-induced cytoskeletal remodeling mediates elevation of cell stiffness and invasiveness in NSCLC","volume":"9","author":"Gladilin","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.taap.2026.117853_bb0035","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1007682","article-title":"A plausible accelerating function of intermediate states in cancer metastasis","volume":"16","author":"Goetz","year":"2020","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.taap.2026.117853_bb0040","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/978-1-4939-9936-1_11","article-title":"Concurrent immunohistochemical localization and western blot analysis of the MIF receptor, CD74, in formalin-fixed, paraffin-embedded tissue","volume":"2080","author":"Graham","year":"2020","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.taap.2026.117853_bb0045","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1124\/jpet.104.082099","article-title":"Inhibition of gene markers of fibrosis with a novel inhibitor of transforming growth factor-beta type I receptor kinase in puromycin-induced nephritis","volume":"313","author":"Grygielko","year":"2005","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.taap.2026.117853_bb0055","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1159\/000147748","article-title":"An overview of epithelio-mesenchymal transformation","volume":"154","author":"Hay","year":"1995","journal-title":"Acta Anat. (Basel)"},{"key":"10.1016\/j.taap.2026.117853_bb0060","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines9070839","article-title":"TGF-\u03b21 receptor inhibitor SB525334 attenuates the epithelial to mesenchymal transition of peritoneal mesothelial cells via the TGF-\u03b21 signaling pathway","volume":"9","author":"Heo","year":"2021","journal-title":"Biomedicines"},{"key":"10.1016\/j.taap.2026.117853_bb0065","doi-asserted-by":"crossref","first-page":"31","DOI":"10.29245\/2689-999X\/2019\/2.1149","article-title":"Epithelial-mesenchymal transition contributes to pulmonary fibrosis via aberrant epithelial\/fibroblastic cross-talk","volume":"3","author":"Hill","year":"2019","journal-title":"J. Lung Health Dis."},{"key":"10.1016\/j.taap.2026.117853_bb0070","doi-asserted-by":"crossref","first-page":"2730","DOI":"10.1091\/mbc.12.9.2730","article-title":"Alpha-smooth muscle actin expression upregulates fibroblast contractile activity","volume":"12","author":"Hinz","year":"2001","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.taap.2026.117853_bb0075","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1186\/s12931-014-0157-3","article-title":"Anti-fibrotic effects of nintedanib in lung fibroblasts derived from patients with idiopathic pulmonary fibrosis","volume":"15","author":"Hostettler","year":"2014","journal-title":"Respir. Res."},{"key":"10.1016\/j.taap.2026.117853_bb0080","doi-asserted-by":"crossref","DOI":"10.3390\/cancers14194845","article-title":"Regulation of TGF- \u03b2 1-induced EMT by autophagy-dependent energy metabolism in cancer cells","volume":"14","author":"Hwang","year":"2022","journal-title":"Cancers (Basel)"},{"key":"10.1016\/j.taap.2026.117853_bb0085","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.resinv.2020.01.003","article-title":"Nintedanib inhibits epithelial-mesenchymal transition in A549 alveolar epithelial cells through regulation of the TGF-\u03b2\/Smad pathway","volume":"58","author":"Ihara","year":"2020","journal-title":"Respir. Investig."},{"key":"10.1016\/j.taap.2026.117853_bb0090","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1002\/jcb.27460","article-title":"Novel TGF-\u03b21 inhibitor antagonizes TGF-\u03b21-induced epithelial-mesenchymal transition in human A549 lung cancer cells","volume":"120","author":"Jeong","year":"2019","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/j.taap.2026.117853_bb0095","doi-asserted-by":"crossref","DOI":"10.1016\/j.pupt.2023.102267","article-title":"Anti-fibrotic effects of nintedanib on lung fibroblasts derived from patients with progressive Fibrosing interstitial lung diseases (PF-ILDs)","volume":"83","author":"Joannes","year":"2023","journal-title":"Pulm. Pharmacol. Ther."},{"key":"10.1016\/j.taap.2026.117853_bb0100","doi-asserted-by":"crossref","first-page":"898","DOI":"10.3346\/jkms.2007.22.5.898","article-title":"Transforming growth factor beta1 induces epithelial-to-mesenchymal transition of A549 cells","volume":"22","author":"Ju","year":"2007","journal-title":"J. Korean Med. Sci."},{"key":"10.1016\/j.taap.2026.117853_bb0105","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1172\/JCI200320530","article-title":"Epithelial-mesenchymal transition and its implications for fibrosis","volume":"112","author":"Kalluri","year":"2003","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.taap.2026.117853_bb0110","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1172\/JCI39104","article-title":"The basics of epithelial-mesenchymal transition","volume":"119","author":"Kalluri","year":"2009","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.taap.2026.117853_bb0115","doi-asserted-by":"crossref","DOI":"10.1186\/1465-9921-14-84","article-title":"Myofibroblast expression in airways and alveoli is affected by smoking and COPD","volume":"14","author":"Karvonen","year":"2013","journal-title":"Respir. Res."},{"key":"10.1016\/j.taap.2026.117853_bb0120","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/1465-9921-6-56","article-title":"TGF-beta1 induces human alveolar epithelial to mesenchymal cell transition (EMT)","volume":"6","author":"Kasai","year":"2005","journal-title":"Respir. Res."},{"key":"10.1016\/j.taap.2026.117853_bb0125","doi-asserted-by":"crossref","first-page":"13180","DOI":"10.1073\/pnas.0605669103","article-title":"Alveolar epithelial cell mesenchymal transition develops in vivo during pulmonary fibrosis and is regulated by the extracellular matrix","volume":"103","author":"Kim","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.taap.2026.117853_bb0130","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1038\/nrm3758","article-title":"Molecular mechanisms of epithelial-mesenchymal transition","volume":"15","author":"Lamouille","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.taap.2026.117853_bb0135","doi-asserted-by":"crossref","first-page":"1658001","DOI":"10.3389\/fmed.2025.1658001","article-title":"Epithelial-mesenchymal transition in pulmonary fibrosis: molecular mechanisms and emerging therapeutic strategies","volume":"12","author":"Li","year":"2025","journal-title":"Front. Med. (Lausanne)"},{"key":"10.1016\/j.taap.2026.117853_bb0140","first-page":"789","article-title":"Nintedanib inhibits TGF-\u03b2-induced myofibroblast transdifferentiation in human Tenon\u2019s fibroblasts","volume":"24","author":"Lin","year":"2018","journal-title":"Mol. Vis."},{"key":"10.1016\/j.taap.2026.117853_bb0145","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.taap.2026.117853_bb0150","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1124\/pr.55.4.4","volume":"55","author":"Neubig","year":"2003","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.taap.2026.117853_bb0155","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1006\/jmbi.2000.3777","article-title":"Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members","volume":"299","author":"Nollet","year":"2000","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.taap.2026.117853_bb0160","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/genes12030352","article-title":"Temporal dynamic methods for bulk RNA-Seq time series data","volume":"12","author":"Oh","year":"2021","journal-title":"Genes (Basel)"},{"key":"10.1016\/j.taap.2026.117853_bb0165","doi-asserted-by":"crossref","first-page":"3861","DOI":"10.1242\/jcs.00059","article-title":"Fibronectin at a glance","volume":"115","author":"Pankov","year":"2002","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.taap.2026.117853_bb0170","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.tcb.2018.12.001","article-title":"EMT transition states during tumor progression and metastasis","volume":"29","author":"Pastushenko","year":"2019","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.taap.2026.117853_bb0175","doi-asserted-by":"crossref","DOI":"10.1016\/j.yexcr.2024.113993","article-title":"Single-cell tracking as a tool for studying EMT-phenotypes","volume":"437","author":"Quinsgaard","year":"2024","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.taap.2026.117853_bb0180","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1186\/s12931-017-0654-2","article-title":"Anti-fibrotic efficacy of nintedanib in pulmonary fibrosis via the inhibition of fibrocyte activity","volume":"18","author":"Sato","year":"2017","journal-title":"Respir. Res."},{"key":"10.1016\/j.taap.2026.117853_bb0185","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1016\/j.bbamcr.2011.07.016","article-title":"Dynamics of TGF-\u03b2 induced epithelial-to-mesenchymal transition monitored by electric cell-substrate impedance sensing","volume":"1813","author":"Schneider","year":"2011","journal-title":"Biochim. Biophys. Acta, Mol. Cell Res."},{"key":"10.1016\/j.taap.2026.117853_bb0190","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0080068","article-title":"Tension monitoring during epithelial-to-mesenchymal transition links the switch of phenotype to expression of moesin and cadherins in NMuMG cells","volume":"8","author":"Schneider","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.taap.2026.117853_bb0195","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1002\/jcb.21154","article-title":"Smooth muscle alpha-actin expression and myofibroblast differentiation by TGFbeta are dependent upon MK2","volume":"100","author":"Sousa","year":"2007","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/j.taap.2026.117853_bb0200","doi-asserted-by":"crossref","unstructured":"Tae, K., Cho, S.W., Lee, S., Heo, D., Cha, H.S., Lee, D.Y., Oh, E., Choi, M., Shin, D., Yang, S., Lee, C., Choi, C.Y. (2025) MARCH2-mediated Lys63-linked polyubiquitination promotes metastasis by modulating the catalytic activity of TGF-\u03b2 type I receptor. Cell Death Dis. 16: 814.","DOI":"10.1038\/s41419-025-08145-3"},{"key":"10.1016\/j.taap.2026.117853_bb0205","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1126\/science.2006419","article-title":"Cadherin cell adhesion receptors as a morphogenetic regulator","volume":"251","author":"Takeichi","year":"1991","journal-title":"Science"},{"key":"10.1016\/j.taap.2026.117853_bb0210","doi-asserted-by":"crossref","first-page":"bio062213","DOI":"10.1242\/bio.062213","article-title":"Epithelial fusion is mediated by a partial epithelial-mesenchymal transition","volume":"14","author":"Tamilkumar","year":"2025","journal-title":"Biol. Open"},{"key":"10.1016\/j.taap.2026.117853_bb0215","doi-asserted-by":"crossref","first-page":"2200474","DOI":"10.1183\/13993003.00474-2022","article-title":"Single-nucleus chromatin accessibility identifies a critical role for TWIST1 in idiopathic pulmonary fibrosis myofibroblast activity","volume":"62","author":"Valenzi","year":"2023","journal-title":"Eur. Respir. J."},{"key":"10.1016\/j.taap.2026.117853_bb0220","doi-asserted-by":"crossref","first-page":"6566","DOI":"10.1093\/nar\/gki965","article-title":"SIP1\/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions","volume":"33","author":"Vandewalle","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.taap.2026.117853_bb0225","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1038\/onc.2010.509","article-title":"Vimentin regulates EMT induction by Slug and oncogenic H-Ras and migration by governing Axl expression in breast cancer","volume":"30","author":"Vuoriluoto","year":"2011","journal-title":"Oncogene"},{"key":"10.1016\/j.taap.2026.117853_bb0230","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.yexcr.2005.11.004","article-title":"Multiple roles of \u03b1-smooth muscle actin in mechanotransduction","volume":"312","author":"Wang","year":"2006","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.taap.2026.117853_bb0235","article-title":"Epithelial-to-mesenchymal transition proceeds through directional destabilization of multidimensional attractor","volume":"11","author":"Wang","year":"2022","journal-title":"Elife"},{"key":"10.1016\/j.taap.2026.117853_bb0240","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1242\/jcs.000455","article-title":"Cadherin switching","volume":"121","author":"Wheelock","year":"2008","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.taap.2026.117853_bb0245","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1038\/cr.2009.5","article-title":"TGF-beta-induced epithelial to mesenchymal transition","volume":"19","author":"Xu","year":"2009","journal-title":"Cell Res."},{"key":"10.1016\/j.taap.2026.117853_bb0250","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1016\/j.ajpath.2016.03.015","article-title":"\u03b13 integrin of cell-cell contact mediates kidney fibrosis by integrin-linked kinase in proximal tubular E-cadherin deficient mice","volume":"186","author":"Zheng","year":"2016","journal-title":"Am. J. Pathol."}],"container-title":["Toxicology and Applied Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041008X26001493?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041008X26001493?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:03:00Z","timestamp":1788235380000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041008X26001493"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":49,"alternative-id":["S0041008X26001493"],"URL":"https:\/\/doi.org\/10.1016\/j.taap.2026.117853","relation":{},"ISSN":["0041-008X"],"issn-type":[{"value":"0041-008X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An innovative label-free approach for investigating epithelial-mesenchymal transition: pharmacological characterization of TGF-\u03b21 effects in A549 cells","name":"articletitle","label":"Article Title"},{"value":"Toxicology and Applied Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.taap.2026.117853","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"117853"}}