{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T03:53:58Z","timestamp":1777434838662,"version":"3.51.4"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Analytical Platform Construction Project of Scientific Production of Sichuan Province, China","award":["13CGPT0049"],"award-info":[{"award-number":["13CGPT0049"]}]},{"name":"Analytical Platform Construction Project of Key Scientific Production of Sichuan Province, China","award":["12CGPT0022"],"award-info":[{"award-number":["12CGPT0022"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2018,2]]},"DOI":"10.1016\/j.talanta.2017.09.085","type":"journal-article","created":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T10:30:45Z","timestamp":1506853845000},"page":"538-544","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["pH detection in biological samples by 1D and 2D 1H\u201331P NMR"],"prefix":"10.1016","volume":"178","author":[{"given":"Ting","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Liao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di","family":"Mu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiandeng","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunchun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengchi","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"6","key":"10.1016\/j.talanta.2017.09.085_bib1","doi-asserted-by":"crossref","first-page":"C1733","DOI":"10.1152\/ajpcell.00578.2003","article-title":"The role of intracellular pH in cell growth arrest induced by ATP","volume":"287","author":"Humez","year":"2004","journal-title":"Am. J. Physiol.-Cell Physiol."},{"issue":"6","key":"10.1016\/j.talanta.2017.09.085_bib2","first-page":"1194","article-title":"Cellular pH gradient in tumor versus normal tissue: potential exploitation for the treatment of cancer","volume":"56","author":"Gerweck","year":"1996","journal-title":"Cancer Res."},{"issue":"8","key":"10.1016\/j.talanta.2017.09.085_bib3","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.2967\/jnumed.109.068981","article-title":"Tumor pH and its measurement","volume":"51","author":"Zhang","year":"2010","journal-title":"J. Nucl. Med."},{"issue":"3","key":"10.1016\/j.talanta.2017.09.085_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/S1053-4296(98)80043-X","article-title":"Tumor pH: implications for treatment and novel drug design","volume":"8","author":"Gerweck","year":"1998","journal-title":"Semin. Radiat. Oncol."},{"issue":"2","key":"10.1016\/j.talanta.2017.09.085_bib5","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1161\/STROKEAHA.114.007896","article-title":"Changes of pH and energy state in subacute human ischemia assessed by multi Nuclear Magnetic Resonance spectroscopy","volume":"46","author":"Zollner","year":"2015","journal-title":"Stroke"},{"issue":"3","key":"10.1016\/j.talanta.2017.09.085_bib6","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/j.nbd.2006.09.019","article-title":"Intracellular pH regulates amyloid precursor protein intracellular domain accumulation","volume":"25","author":"Vingtdeux","year":"2007","journal-title":"Neurobiol. Dis."},{"issue":"2","key":"10.1016\/j.talanta.2017.09.085_bib7","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/BF00584198","article-title":"Intracellular pH and surface pH in skeletal and cardiac muscle measured with a double-barrelled pH microelectrode","volume":"386","author":"Dehemptinne","year":"1980","journal-title":"Pflug. Arch."},{"issue":"5","key":"10.1016\/j.talanta.2017.09.085_bib8","doi-asserted-by":"crossref","first-page":"2709","DOI":"10.1021\/cr900249z","article-title":"Fluorescent indicators for intracellular pH","volume":"110","author":"Han","year":"2009","journal-title":"Chem. Rev."},{"issue":"17","key":"10.1016\/j.talanta.2017.09.085_bib9","doi-asserted-by":"crossref","first-page":"8724","DOI":"10.1021\/acs.analchem.5b01527","article-title":"Ratiometric fluorescent sensing of pH values in living cells by dual-fluorophore-labeled i-motif nanoprobes","volume":"87","author":"Huang","year":"2015","journal-title":"Anal. Chem."},{"issue":"1","key":"10.1016\/j.talanta.2017.09.085_bib10","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0168-1656(99)00203-5","article-title":"Measurement of intracellular pH by 31P NMR in Aspergillus niger","volume":"77","author":"Hesse","year":"2000","journal-title":"J. Biotechnol."},{"issue":"4","key":"10.1016\/j.talanta.2017.09.085_bib11","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1249\/00005768-198708000-00015","article-title":"Phosphorus nuclear magnetic resonance: a non-invasive technique for the study of muscle bioenergetics during exercise","volume":"19","author":"Sapega","year":"1987","journal-title":"Med. Sci. Sports Exerc."},{"issue":"21","key":"10.1016\/j.talanta.2017.09.085_bib12","doi-asserted-by":"crossref","first-page":"7836","DOI":"10.1073\/pnas.85.21.7836","article-title":"Intracellular pH in human skeletal muscle by 1H NMR","volume":"85","author":"Pan","year":"1988","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.talanta.2017.09.085_bib13","first-page":"73","article-title":"An analysis of the pH-dependent chemical shift behavior of phosphorus-containing metabolites","author":"Robitaille","year":"1991","journal-title":"J. Magn. Reson."},{"issue":"2","key":"10.1016\/j.talanta.2017.09.085_bib14","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1093\/aob\/mcn174","article-title":"Response of cytoplasmic pH to anoxia in plant tissues with altered activities of fermentation enzymes: application of methyl phosphonate as an NMR pH probe","volume":"103","author":"Couldwell","year":"2009","journal-title":"Ann. Bot."},{"issue":"2","key":"10.1016\/j.talanta.2017.09.085_bib15","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/0003-2697(81)90504-2","article-title":"Measurement of transmembrane pH gradients in human erythrocytes using 19F NMR","volume":"114","author":"Taylor","year":"1981","journal-title":"Anal. Biochem."},{"issue":"1","key":"10.1016\/j.talanta.2017.09.085_bib16","doi-asserted-by":"crossref","first-page":"C1","DOI":"10.1152\/ajpcell.1982.242.1.C1","article-title":"Application of 31P NMR spectroscopy to the study of striated muscle metabolism","volume":"242","author":"Meyer","year":"1982","journal-title":"Am. J. Physiol.-Cell Physiol."},{"issue":"5","key":"10.1016\/j.talanta.2017.09.085_bib17","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1016\/j.jpba.2010.11.022","article-title":"Electrodeless, accurate pH determination in highly basic media using a new set of 1H NMR pH indicators","volume":"54","author":"Orgovan","year":"2011","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.talanta.2017.09.085_bib18","doi-asserted-by":"crossref","first-page":"2514","DOI":"10.1039\/b802838a","article-title":"19F NMR based pH probes: lanthanide (III) complexes with pH-sensitive chemical shifts","volume":"22","author":"Kenwright","year":"2008","journal-title":"Chem. Commun."},{"issue":"2","key":"10.1016\/j.talanta.2017.09.085_bib19","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1002\/mrm.10384","article-title":"the carnosine C-2 proton's chemical shift reports intracellular pH in oxidative and glycolytic muscle fibers","volume":"49","author":"Damon","year":"2003","journal-title":"Magn. Reson. Med."},{"issue":"2","key":"10.1016\/j.talanta.2017.09.085_bib20","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.aca.2004.07.005","article-title":"1H\/31P NMR pH indicator series to eliminate the glass electrode in NMR spectroscopic pK a determinations","volume":"522","author":"Szak\u00e1cs","year":"2004","journal-title":"Anal. Chim. Acta"},{"issue":"1","key":"10.1016\/j.talanta.2017.09.085_bib21","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.aca.2009.06.047","article-title":"From proton nuclear magnetic resonance spectra to pH. Assessment of 1H NMR pH indicator compound set for deuterium oxide solutions","volume":"648","author":"Tynkkynen","year":"2009","journal-title":"Anal. Chim. Acta"},{"issue":"1","key":"10.1016\/j.talanta.2017.09.085_bib22","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s10858-008-9234-6","article-title":"Internal pH indicators for biomolecular NMR","volume":"41","author":"Baryshnikova","year":"2008","journal-title":"J. Biomol. NMR"},{"issue":"5","key":"10.1016\/j.talanta.2017.09.085_bib23","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1002\/mrm.20034","article-title":"Determination of intracellular pH and compartmentation using diffusion-weighted NMR spectroscopy with pH-sensitive indicators","volume":"51","author":"Cohen","year":"2004","journal-title":"Magn. Reson. Med."},{"issue":"15","key":"10.1016\/j.talanta.2017.09.085_bib24","doi-asserted-by":"crossref","first-page":"6814","DOI":"10.1021\/jm300520q","article-title":"6-Trifluoromethylpyridoxine: novel 19F NMR pH indicator for in vivo detection","volume":"55","author":"Yu","year":"2012","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.talanta.2017.09.085_bib25","first-page":"541","article-title":"New highly sensitive pH probes for 31P NMR spectroscopy. Spin-lattice relaxation time measurements as a function of molecular structure, temperature, pH, and biological medium","volume":"11","author":"Gaelle","year":"2008","journal-title":"Comptes Rendus Chim."},{"issue":"6","key":"10.1016\/j.talanta.2017.09.085_bib26","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1002\/(SICI)1099-1492(199709)10:6<263::AID-NBM472>3.0.CO;2-J","article-title":"Phosphonates as 31P NMR markers of extra and intracellular space and pH in perfused rat liver","volume":"10","author":"Bruynseels","year":"1997","journal-title":"NMR Biomed."},{"issue":"3","key":"10.1016\/j.talanta.2017.09.085_bib27","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1074\/jbc.M008023200","article-title":"Use of diethyl (2-methylpyrrolidin-2-yl) phosphonate as a highly sensitive extra-and intracellular 31P NMR pH indicator in isolated organs direct NMR evidence of acidic compartments in the ischemic and reperfused rat liver","volume":"276","author":"Pietri","year":"2001","journal-title":"J. Biol. Chem."},{"issue":"26","key":"10.1016\/j.talanta.2017.09.085_bib28","doi-asserted-by":"crossref","first-page":"19505","DOI":"10.1074\/jbc.M001784200","article-title":"\u03b1-and \u03b2-phosphorylated amines and pyrrolidines, a new class of low toxic highly sensitive 31P NMR pH indicators -modeling of pka and chemical shift values as a function of substituents","volume":"275","author":"Pietri","year":"2000","journal-title":"J. Biol. Chem."},{"issue":"5","key":"10.1016\/j.talanta.2017.09.085_bib29","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1016\/S0968-0896(01)00414-X","article-title":"Synthesis and 31P NMR characterization of new low toxic highly sensitive pH probes designed for in vivo acidic pH studies","volume":"10","author":"Martel","year":"2002","journal-title":"Bioorg. Med. Chem."},{"issue":"2","key":"10.1016\/j.talanta.2017.09.085_bib30","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.ab.2008.05.052","article-title":"Investigation of subcellular acidic compartments using \u03b1-aminophosphonate 31P nuclear magnetic resonance probes","volume":"380","author":"Gosset","year":"2008","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.talanta.2017.09.085_bib31","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.talanta.2015.12.051","article-title":"Direct and simultaneous quantification of ATP, ADP and AMP by 1H and 31P Nuclear Magnetic Resonance spectroscopy","volume":"150","author":"Lian","year":"2016","journal-title":"Talanta"},{"issue":"38","key":"10.1016\/j.talanta.2017.09.085_bib32","doi-asserted-by":"crossref","first-page":"12360","DOI":"10.1021\/ja063773h","article-title":"DFTMP, an NMR reagent for assessing the near-neutral pH of biological samples","volume":"128","author":"Reily","year":"2006","journal-title":"J. Am. Chem. Soc."},{"issue":"1","key":"10.1016\/j.talanta.2017.09.085_bib33","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1006\/abio.1998.2698","article-title":"Study of imidazole-based Nuclear Magnetic Resonance probes of cellular pH","volume":"261","author":"Gasparovic","year":"1998","journal-title":"Anal. Biochem."},{"issue":"4","key":"10.1016\/j.talanta.2017.09.085_bib34","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.talanta.2003.10.007","article-title":"Accurate determination of low pK values by 1H NMR titration","volume":"62","author":"Szak\u00e1cs","year":"2004","journal-title":"Talanta"},{"issue":"3","key":"10.1016\/j.talanta.2017.09.085_bib35","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1093\/oxfordjournals.jbchem.a134413","article-title":"Intracellular pH determination by a 31P NMR technique. The second dissociation constant of phosphoric acid in a biological system","volume":"94","author":"Seo","year":"1983","journal-title":"J. Biochem."},{"issue":"12","key":"10.1016\/j.talanta.2017.09.085_bib36","doi-asserted-by":"crossref","first-page":"5946","DOI":"10.1021\/ac500966e","article-title":"Evaluation of fast 2D NMR for metabolomics","volume":"86","author":"Guennec","year":"2014","journal-title":"Anal. Chem."},{"issue":"31","key":"10.1016\/j.talanta.2017.09.085_bib37","doi-asserted-by":"crossref","first-page":"12904","DOI":"10.1021\/ja3051052","article-title":"Utilization of SABRE-derived hyperpolarization to detect low-concentration analytes via 1D and 2D NMR methods","volume":"134","author":"Lloyd","year":"2012","journal-title":"J. Am. Chem. Soc."},{"issue":"7","key":"10.1016\/j.talanta.2017.09.085_bib38","doi-asserted-by":"crossref","first-page":"3950","DOI":"10.1021\/es204016h","article-title":"High resolution characterization of organic phosphorus in soil extracts using 2D 1H\u201331P NMR correlation spectroscopy","volume":"46","author":"Vestergren","year":"2012","journal-title":"Environ. Sci. Technol."},{"issue":"24","key":"10.1016\/j.talanta.2017.09.085_bib39","doi-asserted-by":"crossref","first-page":"12197","DOI":"10.1021\/acs.analchem.5b03082","article-title":"Chemical shifts to metabolic pathways: identifying metabolic pathways directly from a single 2D NMR spectrum","volume":"87","author":"Dubey","year":"2015","journal-title":"Anal. Chem."},{"issue":"24","key":"10.1016\/j.talanta.2017.09.085_bib40","doi-asserted-by":"crossref","first-page":"9385","DOI":"10.1021\/ac071583z","article-title":"Method for determining molar concentrations of metabolites in complex solutions from two-dimensional 1H\u201313C NMR spectra","volume":"79","author":"Lewis","year":"2007","journal-title":"Anal. Chem."}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914017310329?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914017310329?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T12:20:19Z","timestamp":1758543619000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914017310329"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2]]},"references-count":40,"alternative-id":["S0039914017310329"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2017.09.085","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2018,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"pH detection in biological samples by 1D and 2D 1H\u201331P NMR","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2017.09.085","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}