{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T18:20:33Z","timestamp":1775672433120,"version":"3.50.1"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2019,9]]},"DOI":"10.1016\/j.talanta.2019.04.046","type":"journal-article","created":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T01:36:42Z","timestamp":1556674602000},"page":"514-519","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["A linear gradient sequential injection chromatography method exploiting programmable fluidics for the determination of three methylxanthines"],"prefix":"10.1016","volume":"202","author":[{"given":"Parthena","family":"Chorti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melina","family":"Ntousikou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasios","family":"Economou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.talanta.2019.04.046_bib1","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1070\/RC2012v081n05ABEH004220","article-title":"Methylxanthines: Properties and determination in various objects","volume":"81","author":"Andreeva","year":"2012","journal-title":"Russ. Chem. Rev."},{"key":"10.1016\/j.talanta.2019.04.046_bib2","first-page":"1","article-title":"Xanthine alkaloids: Occurrence, biosynthesis, and function in plants","volume":"vol. 105","author":"Ashihara","year":"2017"},{"key":"10.1016\/j.talanta.2019.04.046_bib3","doi-asserted-by":"crossref","first-page":"1600670","DOI":"10.1002\/mnfr.201600670","article-title":"Health benefits of methylxanthines in neurodegenerative diseases","volume":"61","author":"O\u00f1atibia-Astibia","year":"2017","journal-title":"Mol. Nutr. Food Res."},{"key":"10.1016\/j.talanta.2019.04.046_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.rmed.2016.01.022","article-title":"The potential of methylxanthine-based therapies in pediatric respiratory tract diseases","volume":"112","author":"O\u00f1atibia-Astibia","year":"2016","journal-title":"Respir. Med."},{"key":"10.1016\/j.talanta.2019.04.046_bib5","doi-asserted-by":"crossref","first-page":"4159","DOI":"10.3390\/nu5104159","article-title":"Health benefits of methylxanthines in cacao and chocolate","volume":"5","author":"Franco","year":"2013","journal-title":"Nutrients"},{"key":"10.1016\/j.talanta.2019.04.046_bib6","doi-asserted-by":"crossref","first-page":"3071","DOI":"10.1039\/c2ay25706h","article-title":"Food analysis \u2013 samples preparation and chromatographic methods in determination of selected biogenic amines, methylxanthines and water-soluble vitamins","volume":"4","author":"Plonka","year":"2012","journal-title":"Anal. Methods"},{"key":"10.1016\/j.talanta.2019.04.046_bib7","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1080\/10739149.2011.651671","article-title":"Monolithic columns in flow analysis: A review of SIC and MSC techniques","volume":"40","author":"Fern\u00e1ndez","year":"2012","journal-title":"Instrum. Sci. Technol."},{"key":"10.1016\/j.talanta.2019.04.046_bib8","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1080\/10408347.2013.848778","article-title":"The second five years of sequential injection chromatography: Significant developments in the technology and methodologies","volume":"44","author":"Idris","year":"2014","journal-title":"Crit. Rev. Anal. Chem."},{"key":"10.1016\/j.talanta.2019.04.046_bib9","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1080\/00032719.2012.749487","article-title":"Flow injection\/sequential injection chromatography: A review of recent developments in low pressure with high performance chemical separation","volume":"46","author":"Hartwell","year":"2013","journal-title":"Anal. Lett."},{"key":"10.1016\/j.talanta.2019.04.046_bib10","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1021\/ac504059c","article-title":"Advances and recent trends in the field of monolithic columns for chromatography","volume":"87","author":"Svec","year":"2015","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2019.04.046_bib11","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1002\/jssc.201501011","article-title":"Current trends in the development of porous polymer monoliths for the separation of small molecules","volume":"39","author":"Urban","year":"2016","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.talanta.2019.04.046_bib12","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0021-9673(03)01239-1","article-title":"Reversed-phase porous silica rods, an alternative approach to high-performance liquid chromatographic separation using the sequential injection chromatography technique","volume":"1015","author":"\u0160atinsky","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.talanta.2019.04.046_bib13","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.aca.2007.02.018","article-title":"An overview of sequential injection chromatography","volume":"600","author":"Chocholou\u0161","year":"2007","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2019.04.046_bib14","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0003-2670(03)00902-4","article-title":"Coupling of monolithic columns with sequential injection technique. A new separation approach in flow methods","volume":"494","author":"Huclov\u00e1","year":"2003","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2019.04.046_bib15","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s12161-017-0982-3","article-title":"Automated sequential injection method for determination of caffeine in coffee drinks","volume":"11","author":"Mihal\u010d\u00edkov\u00e1 L","year":"2018","journal-title":"Food Anal. Methods"},{"key":"10.1016\/j.talanta.2019.04.046_bib16","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1002\/jssc.200301644","article-title":"Sequential injection chromatographic determination of paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets","volume":"27","author":"\u0160at\u00ednsk\u00fd","year":"2004","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.talanta.2019.04.046_bib17","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.chroma.2007.11.046","article-title":"Quasi-flow injection analysis for rapid determination of caffeine in tea using the sample pre-treatment method with a cartridge column filled with polyvinylpolypyrrolidone","volume":"1177","author":"Yamauchi","year":"2008","journal-title":"J. Chromatogr., A"},{"key":"10.1016\/j.talanta.2019.04.046_bib18","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.2116\/analsci.18.1241","article-title":"Simultaneous determination of paracetamol and caffeine by flow injection-solid phase spectrometry using C18 silica gel as a sensing support","volume":"18","author":"Ortega-Barrales","year":"2002","journal-title":"Anal. Sci."},{"key":"10.1016\/j.talanta.2019.04.046_bib19","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.aca.2011.12.002","article-title":"Development of a chromatographic low pressure flow injection system: Application to the analysis of methylxanthines in coffee","volume":"715","author":"Rodrigo Santos","year":"2012","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2019.04.046_bib20","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1007\/s00216-008-1848-8","article-title":"Modulation of mobile phase composition in flow-injection\/sequential-injection chromatography exploiting multisyringe flow analysis","volume":"391","author":"Fern\u00e1ndez","year":"2008","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.talanta.2019.04.046_bib21","doi-asserted-by":"crossref","first-page":"7909","DOI":"10.1021\/jf402447n","article-title":"Online sequential-injection chromatography with stepwise gradient elution: A tool for studying the simultaneous adsorption of herbicides on soil and soil components","volume":"61","author":"de Pr\u00e1 Urio","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.talanta.2019.04.046_bib22","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1016\/j.talanta.2011.01.029","article-title":"Simple automated generation of gradient elution conditions in sequential injection chromatography using monolithic column","volume":"84","author":"Koblov\u00e1","year":"2011","journal-title":"Talanta"},{"key":"10.1016\/j.talanta.2019.04.046_bib23","series-title":"Handbook of HPLC","first-page":"119","article-title":"Gradient elution mode","author":"Jandera","year":"2010"},{"key":"10.1016\/j.talanta.2019.04.046_bib24","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.aca.2007.03.048","article-title":"A hybrid FIA\/HPLC system incorporating monolithic column chromatography","volume":"600","author":"Adcock","year":"2007","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2019.04.046_bib25","doi-asserted-by":"crossref","first-page":"8337","DOI":"10.1039\/C6AY02861F","article-title":"A new flow-injection chromatography method exploiting linear-gradient elution for fast quantitative screening of parabens in cosmetics","volume":"8","author":"Barbatsi","year":"2016","journal-title":"Anal. Methods"},{"key":"10.1016\/j.talanta.2019.04.046_bib26","doi-asserted-by":"crossref","first-page":"109","DOI":"10.3390\/foods6120109","article-title":"Methylxanthine content in commonly consumed foods in Spain and determination of its intake during consumption","volume":"6","author":"Sanchez","year":"2017","journal-title":"Foods"},{"key":"10.1016\/j.talanta.2019.04.046_bib27","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1007\/s10337-014-2697-6","article-title":"Low-pressure chromatographic separation of p-hydroxybenzoates using sequential injection with lab-on-valve system and miniature monolithic column","volume":"77","author":"Hartwell","year":"2014","journal-title":"Chromatographia"},{"key":"10.1016\/j.talanta.2019.04.046_bib28","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.chroma.2014.04.010","article-title":"Separations by gradient elution: Why are steep gradient profiles distorted and what is their impact on resolution in reversed-phase liquid chromatography","volume":"1344","author":"Gritti","year":"2014","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.talanta.2019.04.046_bib29","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.aca.2007.05.040","article-title":"Simultaneous determination of antioxidants, preservatives and sweetener additives in food and cosmetics by flow injection analysis coupled to a monolithic column","volume":"594","author":"Garc\u00eda-Jim\u00e9nez","year":"2007","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2019.04.046_bib30","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/S0731-7085(00)00529-X","article-title":"Guidance for robustness\/ruggedness tests in method validation","volume":"24","author":"Van der Heyden","year":"2001","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.talanta.2019.04.046_bib31","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1002\/jssc.200800634","article-title":"Separation of catechins and methylxanthines in tea samples by capillary electrochromatography","volume":"32","author":"Uysal","year":"2009","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.talanta.2019.04.046_bib32","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/0963-9969(96)00038-5","article-title":"Tea preparation and its influence on methylxanthine concentration","volume":"29","author":"Hicks","year":"1996","journal-title":"Food Res. Int."},{"key":"10.1016\/j.talanta.2019.04.046_bib33","doi-asserted-by":"crossref","first-page":"47114","DOI":"10.1039\/C4RA06412G","article-title":"Simultaneous determination of theophylline, theobromine and caffeine in different tea beverages by graphene-oxide based ultrasonic-assisted dispersive micro solid-phase extraction combined with HPLC-UV","volume":"4","author":"Sereshti","year":"2014","journal-title":"RSC Adv."}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914019304357?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914019304357?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T12:27:37Z","timestamp":1758544057000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914019304357"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":33,"alternative-id":["S0039914019304357"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2019.04.046","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2019,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A linear gradient sequential injection chromatography method exploiting programmable fluidics for the determination of three methylxanthines","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2019.04.046","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}