{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T15:40:38Z","timestamp":1777563638604,"version":"3.51.4"},"reference-count":26,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003010","name":"Ege \u00dcniversitesi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2020,3]]},"DOI":"10.1016\/j.talanta.2019.120558","type":"journal-article","created":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T03:14:29Z","timestamp":1573701269000},"page":"120558","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["LC-MS\/MS measurement of leukocyte cystine; effect of preanalytic factors"],"prefix":"10.1016","volume":"209","author":[{"given":"Erhan","family":"Canbay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ebru Demirel","family":"Sezer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sema Kalkan","family":"U\u00e7ar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmut","family":"\u00c7oker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eser Y\u0131ld\u0131r\u0131m","family":"S\u00f6zmen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.talanta.2019.120558_bib19","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/0002-9343(68)90104-6","article-title":"Cystinosis","volume":"44","author":"Crawhall","year":"1968","journal-title":"Am. J. Med."},{"key":"10.1016\/j.talanta.2019.120558_bib20","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1056\/NEJMra020552","article-title":"Cystinosis","volume":"347","author":"Gahl","year":"2002","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.talanta.2019.120558_bib21","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1006\/mgme.2000.3062","article-title":"Corneal crystals in nephropathic cystinosis: natural history and treatment with cysteamine eyedrops","volume":"71","author":"Gahl","year":"2000","journal-title":"Mol. Genet. Metab."},{"key":"10.1016\/j.talanta.2019.120558_bib22","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1007\/PL00013448","article-title":"Early development of the renal lesions in infantile cystinosis.","volume":"15","author":"Mahoney,","year":"2000","journal-title":"Pediatr. Nephrol."},{"key":"10.1016\/j.talanta.2019.120558_bib24","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.clinbiochem.2012.10.005","article-title":"Improvement of the cystine measurement in granulocytes by liquid chromatograhy-tandem mass spectrometry","volume":"46","author":"Garcia-Villoria","year":"2013","journal-title":"Clin. Biochem"},{"key":"10.1016\/j.talanta.2019.120558_bib25","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1056\/NEJM198704163161602","article-title":"Cysteamine therapy for children with nephropathic cystinosis","volume":"316","author":"Gahl","year":"1987","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.talanta.2019.120558_bib26","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1007\/s00467-007-0650-8","article-title":"Nephropathic cystinosis: late complications of a multisystemic disease","volume":"23","author":"Nesterova","year":"2008","journal-title":"Pediatr Nephrol."},{"key":"10.1016\/j.talanta.2019.120558_bib27","doi-asserted-by":"crossref","first-page":"486","DOI":"10.2298\/SARH1108486P","article-title":"Infantile nephropathic cystinosis","volume":"139","author":"Peco-Anti\u0107","year":"2011","journal-title":"Srp. Arh. Celok. Lek."},{"key":"10.1016\/j.talanta.2019.120558_bib28","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1002\/(SICI)1098-1004(199912)14:6<454::AID-HUMU2>3.0.CO;2-H","article-title":"CTNS mutations in patients with cystinosis","volume":"14","author":"Anikster","year":"1999","journal-title":"Hum. Mutat."},{"key":"10.1016\/j.talanta.2019.120558_bib29","series-title":"Genetic Diseases of the Kidney","first-page":"661","article-title":"Hereditary Cystinosis","author":"Kalatzis","year":"2009"},{"key":"10.1016\/j.talanta.2019.120558_bib30","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1093\/hmg\/ddh152","article-title":"Molecular pathogenesis of cystinosis: Effect of CTNS mutations on the transport activity and subcellular localization of cystinosin","volume":"13","author":"Kalatzis","year":"2004","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.talanta.2019.120558_bib31","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1038\/ki.2011.301","article-title":"Cysteamine therapy: a treatment for cystinosis, not a cure","volume":"81","author":"Cherqui","year":"2012","journal-title":"Kidney Int."},{"key":"10.1016\/j.talanta.2019.120558_bib32","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1056\/NEJM199304223281604","article-title":"Improved renal function in children with cystinosis treated with cysteamine","volume":"328","author":"Markello","year":"1993","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.talanta.2019.120558_bib33","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/s00467-010-1627-6","article-title":"Cystinosis: Practical tools for diagnosis and treatment","volume":"26","author":"Wilmer","year":"2011","journal-title":"Pediatr. Nephrol."},{"key":"10.1016\/j.talanta.2019.120558_bib34","doi-asserted-by":"crossref","first-page":"5940","DOI":"10.1093\/emboj\/20.21.5940","article-title":"Cystinosin, the protein defective in cystinosis, is a H+-driven lysosomal cystine transporter","volume":"20","author":"Kalatzis","year":"2001","journal-title":"EMBO J."},{"key":"10.1016\/j.talanta.2019.120558_bib36","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/S0022-3476(69)80017-X","article-title":"The early diagnosis of cystinosis","volume":"74","author":"Schneider JA","year":"1969","journal-title":"J Pediatr."},{"key":"10.1016\/j.talanta.2019.120558_bib37","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1016\/0955-2863(91)90066-E","article-title":"Ion-exchange chromatography for physiological fluid amino acid analysis","volume":"2","author":"Huang","year":"1991","journal-title":"J. Nutr. Biochem."},{"key":"10.1016\/j.talanta.2019.120558_bib38","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0003-2697(71)90081-9","article-title":"A lithium buffer system for accelerated single-column amino acid analysis in physiological fluids https:\/\/doi.org\/10.1016\/0003-2697(71)90081-9","volume":"40","author":"Kedenburg","year":"1971","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.talanta.2019.120558_bib23","doi-asserted-by":"crossref","first-page":"2224","DOI":"10.1093\/clinchem\/45.12.2224","article-title":"New method for determining cystine in leukocytes and fibroblasts","volume":"45","author":"De Graaf-Hess","year":"1999","journal-title":"Clin. Chem."},{"key":"10.1016\/j.talanta.2019.120558_bib39","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1016\/j.clinbiochem.2007.02.005","article-title":"Measurement of cystine in granulocytes using liquid chromatography-tandem mass spectrometry","volume":"40","author":"Chabli","year":"2007","journal-title":"Clin. Biochem."},{"key":"10.1016\/j.talanta.2019.120558_bib41","first-page":"1","article-title":"The lowry method for protein quantitation","volume":"1","author":"Waterborg","year":"1984","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.talanta.2019.120558_bib43","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S1570-0232(02)00914-5","article-title":"Optimization of protein precipitation based upon effectiveness of protein removal and ionization effect in liquid chromatography-tandem mass spectrometry","volume":"785","author":"Polson","year":"2003","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci"},{"key":"10.1016\/j.talanta.2019.120558_bib44","series-title":"High Throughput Bioanalytical Sample Preparation","first-page":"199","article-title":"Protein precipitation: High throughput techniques and strategies for method development,","volume":"5","author":"Wells","year":"2003"},{"key":"10.1016\/j.talanta.2019.120558_bib45","series-title":"Proteomic Profiling and Analytical Chemistry (Second Edition)","first-page":"52","article-title":"Protein Extraction and Precipitation","author":"Nov\u00e1k","year":"2016"},{"key":"10.1016\/j.talanta.2019.120558_bib46","first-page":"993","article-title":"Trichloroacetic acid (TCA) precipitation of proteins","volume":"6","author":"Link","year":"2011","journal-title":"Cold Spring Harb. Protoc."},{"key":"10.1016\/j.talanta.2019.120558_bib47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4172\/2155-9872.S4-001","article-title":"LC-MS\/MS Bioanalysis Method Development, Validation, and Sample Analysis: Points to Consider When Conducting Nonclinical and Clinical Studies in Accordance with Current Regulatory Guidances","volume":"1","author":"Whitmire","year":"2011","journal-title":"J. Anal. Bioanal. Tech."}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914019311919?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914019311919?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T12:31:02Z","timestamp":1758544262000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914019311919"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3]]},"references-count":26,"alternative-id":["S0039914019311919"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2019.120558","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2020,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"LC-MS\/MS measurement of leukocyte cystine; effect of preanalytic factors","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2019.120558","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"120558"}}