{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T06:15:27Z","timestamp":1778652927334,"version":"3.51.4"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100019081","name":"Science and Technology Program of Hunan Province","doi-asserted-by":"publisher","award":["2016WK2002"],"award-info":[{"award-number":["2016WK2002"]}],"id":[{"id":"10.13039\/501100019081","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019081","name":"Science and Technology Program of Hunan Province","doi-asserted-by":"publisher","award":["2016RS2009"],"award-info":[{"award-number":["2016RS2009"]}],"id":[{"id":"10.13039\/501100019081","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21877029"],"award-info":[{"award-number":["21877029"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21890744"],"award-info":[{"award-number":["21890744"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2020,11]]},"DOI":"10.1016\/j.talanta.2020.121286","type":"journal-article","created":{"date-parts":[[2020,6,13]],"date-time":"2020-06-13T15:52:14Z","timestamp":1592063534000},"page":"121286","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Engineering of a dual-site molecular probe for logical bioimaging of lysosomal H2S and pH"],"prefix":"10.1016","volume":"219","author":[{"given":"Yongchao","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingjun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Bing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.talanta.2020.121286_bib1","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1038\/nrm2217","article-title":"Lysosomes: fusion and function","volume":"8","author":"Luzio","year":"2007","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.talanta.2020.121286_bib2","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1093\/jmcb\/mjt022","article-title":"The lysosome: from waste bag to potential therapeutic target","volume":"5","author":"Appelqvist","year":"2013","journal-title":"J. Mol. Cell Biol."},{"key":"10.1016\/j.talanta.2020.121286_bib3","doi-asserted-by":"crossref","first-page":"7007","DOI":"10.1038\/ncomms8007","article-title":"Autophagosome-lysosome fusion is independent of V-ATPase-mediated acidification","volume":"6","author":"Mauvezin","year":"2015","journal-title":"Nat. Commun."},{"key":"10.1016\/j.talanta.2020.121286_bib4","doi-asserted-by":"crossref","first-page":"3587","DOI":"10.1073\/pnas.93.8.3587","article-title":"Mutant cystic fibrosis transmembrane conductance regulator inhibits acidification and apoptosis in C127 cells: possible relevance to cystic fibrosis","volume":"93","author":"Gottlieb","year":"1996","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"key":"10.1016\/j.talanta.2020.121286_bib5","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1073\/pnas.93.2.654","article-title":"Apoptosis induced in Jurkat cells by several agents is preceded by intracellular acidification","volume":"93","author":"Gottlieb","year":"1996","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"key":"10.1016\/j.talanta.2020.121286_bib6","doi-asserted-by":"crossref","first-page":"9842","DOI":"10.1074\/jbc.273.16.9842","article-title":"Lysosomal enzyme trafficking between phagosomes, endosomes, and lysosomes in J774 macrophages","volume":"273","author":"Claus","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.talanta.2020.121286_bib7","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.cell.2012.08.036","article-title":"Lysosomal calcium homeostasis defects, not proton pump defects, cause endo-lysosomal dysfunction in PSEN-defcient cells","volume":"151","author":"Wang","year":"2012","journal-title":"Cell"},{"key":"10.1016\/j.talanta.2020.121286_bib8","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1083\/jcb.201201076","article-title":"TPC proteins are phosphoinositide activated sodium-selective ion channels in endosomes and lysosomes","volume":"198","author":"Coen","year":"2012","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.talanta.2020.121286_bib9","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/nrm3391","article-title":"H2S signalling through protein sulfhydration and beyondNat","volume":"13","author":"Paul","year":"2012","journal-title":"Rev. Mol. Cell Biol."},{"key":"10.1016\/j.talanta.2020.121286_bib10","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1113\/expphysiol.2011.057455","volume":"96","author":"Kimura","year":"2011","journal-title":"Exp. Physiol. Hydrogen sulfide: its production and functions"},{"key":"10.1016\/j.talanta.2020.121286_bib11","first-page":"113","article-title":"Amino acids, hydrogen sulfide: its production","volume":"41","author":"Kimura","year":"2010","journal-title":"release and functions"},{"key":"10.1016\/j.talanta.2020.121286_bib12","first-page":"348","article-title":"New roles for cysteine and transsulfuration enzymes: production of H2S, a neuromodulator and smooth muscle relaxant","volume":"62","author":"Dominy","year":"2004","journal-title":"Nutr. Rev."},{"key":"10.1016\/j.talanta.2020.121286_bib13","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.ejphar.2012.09.039","article-title":"The role of endogenous hydrogen sulfide in pathogenesis of chronotropic dysfunction in rats with cirrhosis","volume":"696","author":"Babaei-Karamshahlou","year":"2012","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.talanta.2020.121286_bib14","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1089\/ars.2011.4451","article-title":"Antioxid. Roles of hydrogen sulfide in the pathogenesis of diabetes mellitus and its complications","volume":"17","author":"Szabo","year":"2012","journal-title":"Redox Signaling"},{"key":"10.1016\/j.talanta.2020.121286_bib15","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1186\/1742-2094-9-202","article-title":"Hydrogen sulfide attenuates spatial memory impairment and hippocampal neuroinflammation in beta-amyloid rat model of Alzheimer's disease","volume":"9","author":"Xuan","year":"2012","journal-title":"J. Neuroinflammation"},{"key":"10.1016\/j.talanta.2020.121286_bib16","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1111\/j.1582-4934.2008.00318.x","article-title":"H2S-induced pancreatic acinar cell apoptosis is mediated via JNK and p38 MAP kinase","volume":"12","author":"Adhikari","year":"2008","journal-title":"J. Cell Mol. Med."},{"key":"10.1016\/j.talanta.2020.121286_bib17","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1096\/fj.04-2279fje","article-title":"Hydrogen sulfide-induced apoptosis of human aorta smooth muscle cells via the activation of mitogen activated protein kinases and caspase-3","volume":"18","author":"Yang","year":"2004","journal-title":"Faseb. J."},{"key":"10.1016\/j.talanta.2020.121286_bib18","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s11022-004-8228-3","article-title":"Analysis of cytosolic and lysosomal pH in apoptotic cells by flow cytometry","volume":"25","author":"Nilsson","year":"2004","journal-title":"Methods Cell Sci."},{"key":"10.1016\/j.talanta.2020.121286_bib19","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.cell.2011.10.026","article-title":"Autophagy: renovation of cells and tissues","volume":"147","author":"Mizushima","year":"2011","journal-title":"Cell"},{"key":"10.1016\/j.talanta.2020.121286_bib20","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.ab.2003.09.037","article-title":"Hydrogen sulfide consumption measured at low steady state concentrations using a sulfidostat","volume":"324","author":"Searcy","year":"2004","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.talanta.2020.121286_bib21","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.chroma.2016.12.035","article-title":"Effect of mobile phase additives on solute retention at low aqueous pH in hydrophilic interaction liquid chromatography","volume":"1483","author":"McCalley","year":"2017","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.talanta.2020.121286_bib22","doi-asserted-by":"crossref","first-page":"2310","DOI":"10.1021\/ol400973v","article-title":"A lysosome-targetable fluorescent probe for imaging hydrogen sulfide in living cells","volume":"15","author":"Liu","year":"2013","journal-title":"Org. Lett."},{"key":"10.1016\/j.talanta.2020.121286_bib23","doi-asserted-by":"crossref","first-page":"2113","DOI":"10.1021\/acs.analchem.5b03634","article-title":"Micro electrochemical pH sensor applicable for real-time ratiometric monitoring of pH values in rat brains","volume":"88","author":"Zhou","year":"2016","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2020.121286_bib24","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.biomaterials.2018.01.009","article-title":"Realizing highly chemoselective detection of H2S in vitro and in vivo with fluorescent probes inside core-shell silica nanoparticles","volume":"159","author":"Wang","year":"2018","journal-title":"Biomaterials"},{"key":"10.1016\/j.talanta.2020.121286_bib25","doi-asserted-by":"crossref","first-page":"4185","DOI":"10.1039\/C4CS00280F","article-title":"Small molecule-based ratiometric fluorescence probes for cations, anions, and biomolecules","volume":"44","author":"Lee","year":"2015","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.talanta.2020.121286_bib26","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1021\/cr300508p","article-title":"Design strategies for water-soluble small molecular chromogenic and fluorogenic probes","volume":"114","author":"Li","year":"2014","journal-title":"Chem. Rev."},{"key":"10.1016\/j.talanta.2020.121286_bib27","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1021\/ar1000633","article-title":"Target-cancer-cell-specific activatable fluorescence imaging probes: rational design and in vivo applications","volume":"44","author":"Kobayashi","year":"2010","journal-title":"Acc. Chem. Res."},{"key":"10.1016\/j.talanta.2020.121286_bib28","doi-asserted-by":"crossref","first-page":"9917","DOI":"10.1002\/anie.201902272","article-title":"A synthetic fluorescent mitochondria-targeted sensor for ratiometric imaging of calcium in live cells","volume":"58","author":"Pendin","year":"2019","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.talanta.2020.121286_bib29","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.cbpa.2009.10.014","article-title":"A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells","volume":"14","author":"Dickinson","year":"2010","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.talanta.2020.121286_bib30","doi-asserted-by":"crossref","first-page":"11943","DOI":"10.1002\/anie.201805955","article-title":"ROS-responsive N-alkylaminoferrocenes for cancer-cell-specific targeting of mitochondria","volume":"57","author":"Reshetnikov","year":"2018","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.talanta.2020.121286_bib31","doi-asserted-by":"crossref","first-page":"5930","DOI":"10.1021\/jacs.5b00042","article-title":"Development of targetable two-photon fluorescent probes to image hypochlorous acid in mitochondria and lysosome in live cell and inflamed mouse model","volume":"137","author":"Yuan","year":"2015","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.talanta.2020.121286_bib32","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1038\/nm.1854","article-title":"Selective molecular imaging of viable cancer cells with pH-activatable fluorescence probes","volume":"15","author":"Urano","year":"2009","journal-title":"Nat. Med."},{"key":"10.1016\/j.talanta.2020.121286_bib33","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.aca.2019.05.057","article-title":"Molecular engineering of a colorimetric two-photon fluorescent probe for visualizing H2S level in lysosome and tumor","volume":"1077","author":"Luo","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2020.121286_bib34","doi-asserted-by":"crossref","first-page":"5563","DOI":"10.1039\/C5AN00683J","article-title":"An efficient ratiometric fluorescent probe for tracking dynamic changes in lysosomal pH","volume":"140","author":"Liu","year":"2015","journal-title":"Analyst"},{"key":"10.1016\/j.talanta.2020.121286_bib35","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1021\/ac503806w","article-title":"Toward a selective, sensitive, fast-responsive, and biocompatible two-photon probe for hydrogen sulfide in live cells","volume":"87","author":"Singha","year":"2015","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2020.121286_bib36","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1038\/364042a0","article-title":"A molecular photoionic AND gate based on fluorescent signalling","volume":"364","author":"de Silva","year":"1993","journal-title":"Nature"},{"key":"10.1016\/j.talanta.2020.121286_bib37","doi-asserted-by":"crossref","first-page":"11132","DOI":"10.1021\/ja205081s","article-title":"Ratiometric detection of mitochondrial thiols with a two-photon fluorescent probe","volume":"133","author":"Lim","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.talanta.2020.121286_bib38","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/235050a0","article-title":"Lysosomes, pH and the anti-malarial action of chloroquine","volume":"235","author":"Homewood","year":"1972","journal-title":"Nature"}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914020305774?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914020305774?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:47:34Z","timestamp":1777445254000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914020305774"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":38,"alternative-id":["S0039914020305774"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2020.121286","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2020,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Engineering of a dual-site molecular probe for logical bioimaging of lysosomal H2S and pH","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2020.121286","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"121286"}}