{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T23:38:25Z","timestamp":1780616305753,"version":"3.54.1"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2022,8]]},"DOI":"10.1016\/j.talanta.2022.123497","type":"journal-article","created":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T21:02:12Z","timestamp":1650574932000},"page":"123497","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["An ultrasensitive UHPLC-ESI-MS\/MS method augmented with a controlled microwave derivatization reaction for quantitation of vitamin D3 and its major metabolites in COVID-19 patients"],"prefix":"10.1016","volume":"246","author":[{"given":"Sameh A.","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0586-1526","authenticated-orcid":false,"given":"Hani M.J.","family":"Khojah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sultan S.","family":"Al-Thagfan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6578-385X","authenticated-orcid":false,"given":"Yaser M.","family":"Alahmadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasser A.","family":"Mohammed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"11","key":"10.1016\/j.talanta.2022.123497_bib1","doi-asserted-by":"crossref","first-page":"1988","DOI":"10.3390\/jcm8111988","article-title":"Elevated serum 25-hydroxyvitamin D levels are associated with improved bone formation and micro-structural measures in elderly hip fracture patients","volume":"8","author":"Sharma","year":"2019","journal-title":"J. Clin. Med."},{"issue":"1","key":"10.1016\/j.talanta.2022.123497_bib2","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/jcb.22661","article-title":"The biology and pathology of vitamin D control in bone","volume":"111","author":"Bell","year":"2010","journal-title":"J. Cell. Biochem."},{"issue":"2","key":"10.1016\/j.talanta.2022.123497_bib3","doi-asserted-by":"crossref","first-page":"491s","DOI":"10.1093\/ajcn\/88.2.491S","article-title":"From vitamin D to hormone D: fundamentals of the vitamin D endocrine system essential for good health","volume":"88","author":"Norman","year":"2008","journal-title":"Am. J. Clin. Nutr."},{"issue":"2","key":"10.1016\/j.talanta.2022.123497_bib4","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.annepidem.2007.12.001","article-title":"Vitamin D status: measurement, interpretation, and clinical application","volume":"19","author":"Holick","year":"2009","journal-title":"Ann. Epidemiol."},{"key":"10.1016\/j.talanta.2022.123497_bib5","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1186\/1475-2891-9-65","article-title":"Vitamin D in health and disease: current perspectives","volume":"9","author":"Zhang","year":"2010","journal-title":"Nutr. J."},{"key":"10.1016\/j.talanta.2022.123497_bib6","doi-asserted-by":"crossref","first-page":"102073","DOI":"10.1016\/j.pupt.2021.102073","article-title":"Impacts of deficiency in vitamin D derivatives on disease severity in adult bronchial asthma patients","author":"Al-Thagfan","year":"2021","journal-title":"Pulm. Pharmacol. Therapeut."},{"issue":"3","key":"10.1016\/j.talanta.2022.123497_bib7","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.chembiol.2013.12.016","article-title":"Vitamin D metabolism, mechanism of action, and clinical applications","volume":"21","author":"Bikle","year":"2014","journal-title":"Chem. Biol."},{"issue":"5","key":"10.1016\/j.talanta.2022.123497_bib8","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1111\/j.1365-2265.1979.tb03101.x","article-title":"Metabolism of intravenously administered cholecalciferol in man","volume":"11","author":"Barragry","year":"1979","journal-title":"Clin. Endocrinol."},{"issue":"10","key":"10.1016\/j.talanta.2022.123497_bib9","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1016\/j.jiph.2020.06.021","article-title":"Role of vitamin D in preventing of COVID-19 infection, progression and severity","volume":"13","author":"Ali","year":"2020","journal-title":"J. Infect. Public Health"},{"key":"10.1016\/j.talanta.2022.123497_bib10","doi-asserted-by":"crossref","first-page":"198148","DOI":"10.1016\/j.virusres.2020.198148","article-title":"Association of vitamin D with the modulation of the disease severity in COVID-19","volume":"289","author":"Mardani","year":"2020","journal-title":"Virus Res."},{"issue":"1","key":"10.1016\/j.talanta.2022.123497_bib11","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1186\/s12879-021-06281-7","article-title":"Vitamin D and COVID-19 severity and related mortality: a prospective study in Italy","volume":"21","author":"Campi","year":"2021","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.talanta.2022.123497_bib12","doi-asserted-by":"crossref","first-page":"100967","DOI":"10.1016\/j.eclinm.2021.100967","article-title":"Influence of 25-hydroxy-cholecalciferol levels on SARS-CoV-2 infection and COVID-19 severity: a systematic review and meta-analysis","volume":"37","author":"Crafa","year":"2021","journal-title":"EClinicalMedicine"},{"issue":"1","key":"10.1016\/j.talanta.2022.123497_bib13","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s00223-019-00620-2","article-title":"Vitamin D measurement, the debates continue, new analytes have emerged, developments have variable outcomes","volume":"106","author":"Fraser","year":"2020","journal-title":"Calcif. Tissue Int."},{"issue":"2","key":"10.1016\/j.talanta.2022.123497_bib14","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1007\/s00216-010-3993-0","article-title":"Quantitative determination of vitamin D metabolites in plasma using UHPLC-MS\/MS","volume":"398","author":"Ding","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"issue":"6","key":"10.1016\/j.talanta.2022.123497_bib15","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1093\/jalm\/jfaa070","article-title":"Improved recognition of 25-hydroxyvitamin D2 by 2 automated immunoassays","volume":"5","author":"Geno","year":"2020","journal-title":"J. Appl. Lab Med."},{"issue":"3","key":"10.1016\/j.talanta.2022.123497_bib16","doi-asserted-by":"crossref","first-page":"186","DOI":"10.3164\/jcbn.15-61","article-title":"Performance evaluation of two immunoassays for 25-hydroxyvitamin D","volume":"58","author":"Li","year":"2016","journal-title":"J. Clin. Biochem. Nutr."},{"key":"10.1016\/j.talanta.2022.123497_bib17","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/1475-2891-10-46","article-title":"Misleading measures in Vitamin D analysis: a novel LC-MS\/MS assay to account for epimers and isobars","volume":"10","author":"Shah","year":"2011","journal-title":"Nutr. J."},{"issue":"5","key":"10.1016\/j.talanta.2022.123497_bib18","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1002\/bmc.1130090508","article-title":"Quantitative determination of 25-hydroxyvitamin D3 3-sulphate in human plasma using high performance liquid chromatography","volume":"9","author":"Shimada","year":"1995","journal-title":"Biomed. Chromatogr."},{"issue":"2","key":"10.1016\/j.talanta.2022.123497_bib19","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1002\/jms.1190300218","article-title":"Mass fragmentographic assay for 25-hydroxyvitamin D in plasma without derivatization: enhanced sensitivity for metabolites of vitamins D2 and D3 after pre-column dehydration","volume":"30","author":"Coldwell","year":"1995","journal-title":"J. Mass Spectrom."},{"key":"10.1016\/j.talanta.2022.123497_bib20","doi-asserted-by":"crossref","first-page":"99","DOI":"10.21037\/jlpm.2018.11.06","article-title":"Measurement of vitamin D metabolites by mass spectrometry, an analytical challenge","volume":"3","author":"Zelzer","year":"2018","journal-title":"J. Lab. Precis Med."},{"issue":"1","key":"10.1016\/j.talanta.2022.123497_bib21","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0021-9673(93)80625-I","article-title":"Characterization of vitamin D3 metabolites using continuous-flow fast atom bombardment tandem mass spectrometry and high-performance liquid chromatography","volume":"645","author":"Yeung","year":"1993","journal-title":"J. Chromatogr."},{"issue":"4","key":"10.1016\/j.talanta.2022.123497_bib22","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1002\/bmc.1130050404","article-title":"Analysis of vitamin D and its metabolites using thermospray liquid chromatography\/mass spectrometry","volume":"5","author":"Watson","year":"1991","journal-title":"Biomed. Chromatogr."},{"key":"10.1016\/j.talanta.2022.123497_bib23","doi-asserted-by":"crossref","first-page":"122654","DOI":"10.1016\/j.jchromb.2021.122654","article-title":"LC-MS\/MS analysis of vitamin D3 metabolites in human serum using a salting-out based liquid-liquid extraction and DAPTAD derivatization","volume":"1173","author":"Alshabrawy","year":"2021","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.talanta.2022.123497_bib24","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.jchromb.2014.01.045","article-title":"Development of a sensitive LC\/MS\/MS method for vitamin D metabolites: 1,25 Dihydroxyvitamin D2&3 measurement using a novel derivatization agent","volume":"953\u2013954","author":"Hedman","year":"2014","journal-title":"J. Chromatogr. B"},{"issue":"1","key":"10.1016\/j.talanta.2022.123497_bib25","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.ab.2011.06.043","article-title":"Simultaneous measurement of plasma vitamin D(3) metabolites, including 4\u03b2,25-dihydroxyvitamin D(3), using liquid chromatography-tandem mass spectrometry","volume":"418","author":"Wang","year":"2011","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.talanta.2022.123497_bib26","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.trac.2016.02.005","article-title":"Analysis of vitamin D metabolites by liquid chromatography-tandem mass spectrometry","volume":"84","author":"Ouweland","year":"2016","journal-title":"Trends Anal. Chem."},{"issue":"15","key":"10.1016\/j.talanta.2022.123497_bib27","doi-asserted-by":"crossref","first-page":"7964","DOI":"10.1021\/acs.analchem.5b01861","article-title":"Candidate reference measurement procedure for the determination of (24r),25-dihydroxyvitamin D3 in human serum using isotope-dilution liquid chromatography\u2013tandem mass spectrometry","volume":"87","author":"Tai","year":"2015","journal-title":"Anal. Chem."},{"issue":"1","key":"10.1016\/j.talanta.2022.123497_bib28","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1002\/jssc.201000410","article-title":"Development and optimization of an LC-MS\/MS-based method for simultaneous quantification of vitamin D2 , vitamin D3 , 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3","volume":"34","author":"Adamec","year":"2011","journal-title":"J. Separ. Sci."},{"issue":"4","key":"10.1016\/j.talanta.2022.123497_bib29","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1002\/jms.1904","article-title":"Comparison of APPI, APCI and ESI for the LC-MS\/MS analysis of bezafibrate, cyclophosphamide, enalapril, methotrexate and orlistat in municipal wastewater","volume":"46","author":"Garcia-Ac","year":"2011","journal-title":"J. Mass Spectrom."},{"issue":"5","key":"10.1016\/j.talanta.2022.123497_bib30","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1007\/s00216-008-2095-8","article-title":"Metabolic profiling of major vitamin D metabolites using Diels\u2013Alder derivatization and ultra-performance liquid chromatography\u2013tandem mass spectrometry","volume":"391","author":"Aronov","year":"2008","journal-title":"Anal. Bioanal. Chem."},{"issue":"7","key":"10.1016\/j.talanta.2022.123497_bib31","doi-asserted-by":"crossref","first-page":"2567","DOI":"10.1210\/jc.2013-4388","article-title":"Clinical utility of simultaneous quantitation of 25-hydroxyvitamin D and 24,25-dihydroxyvitamin D by LC-MS\/MS involving derivatization with DMEQ-TAD","volume":"99","author":"Kaufmann","year":"2014","journal-title":"J. Clin. Endocrinol. Metab."},{"issue":"4","key":"10.1016\/j.talanta.2022.123497_bib32","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1194\/jlr.D073536","article-title":"A new sensitive LC\/MS\/MS analysis of vitamin D metabolites using a click derivatization reagent, 2-nitrosopyridine","volume":"58","author":"Wan","year":"2017","journal-title":"J. Lipid Res."},{"issue":"31","key":"10.1016\/j.talanta.2022.123497_bib33","doi-asserted-by":"crossref","first-page":"5875","DOI":"10.1016\/j.chroma.2009.06.035","article-title":"An evaluation of microwave-assisted derivatization procedures using hyphenated mass spectrometric techniques","volume":"1216","author":"Damm","year":"2009","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.talanta.2022.123497_bib34","doi-asserted-by":"crossref","first-page":"532467","DOI":"10.1155\/2014\/532467","article-title":"Microwave-assisted synthesis and characterization of certain oximes, hydrazones, and olefins derived from \u03b2-Keto sulfones","volume":"2014","author":"Abdel-Aziz","year":"2014","journal-title":"J. Chem."},{"key":"10.1016\/j.talanta.2022.123497_bib35","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.aca.2018.09.059","article-title":"Controlled microwave derivatization reaction for reproducible trace analysis of budesonide in human plasma","volume":"1048","author":"Ahmed","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2022.123497_bib36","series-title":"Guidance for Industry on Bioanalytical Method Validation, Fed","year":"2001"},{"issue":"10","key":"10.1016\/j.talanta.2022.123497_bib37","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1002\/anie.198007791","article-title":"Mechanistic aspects of diels-alder reactions: a critical survey","volume":"19","author":"Sauer","year":"1980","journal-title":"Angew Chem. Int. Ed. Engl."},{"issue":"46","key":"10.1016\/j.talanta.2022.123497_bib38","doi-asserted-by":"crossref","first-page":"6250","DOI":"10.1002\/anie.200400655","article-title":"Controlled microwave heating in modern organic synthesis","volume":"43","author":"Kappe","year":"2004","journal-title":"Angew Chem. Int. Ed. Engl."},{"issue":"1","key":"10.1016\/j.talanta.2022.123497_bib39","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1021\/co100015b","article-title":"Open vessel and cooling while heating microwave-assisted synthesis of Pyridinyl N-aryl hydrazones","volume":"13","author":"La Regina","year":"2011","journal-title":"ACS Comb. Sci."},{"issue":"2","key":"10.1016\/j.talanta.2022.123497_bib40","doi-asserted-by":"crossref","first-page":"141","DOI":"10.11613\/BM.2015.015","article-title":"Understanding Bland altman analysis","volume":"25","author":"Giavarina","year":"2015","journal-title":"Biochem. Med."}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914022002934?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914022002934?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T13:01:19Z","timestamp":1758546079000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914022002934"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8]]},"references-count":40,"alternative-id":["S0039914022002934"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2022.123497","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2022,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An ultrasensitive UHPLC-ESI-MS\/MS method augmented with a controlled microwave derivatization reaction for quantitation of vitamin D3 and its major metabolites in COVID-19 patients","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2022.123497","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"123497"}}