{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T17:09:02Z","timestamp":1785863342942,"version":"3.56.0"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY18H300003"],"award-info":[{"award-number":["LY18H300003"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFC0908600"],"award-info":[{"award-number":["2017YFC0908600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2019FZA7017"],"award-info":[{"award-number":["2019FZA7017"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81573389"],"award-info":[{"award-number":["81573389"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2022,10]]},"DOI":"10.1016\/j.talanta.2022.123644","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T11:20:43Z","timestamp":1654082443000},"page":"123644","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"special_numbering":"C","title":["RT-LAMP assay combining multi-fluorescent probes for SARS-CoV-2 RNA detection and variant differentiation"],"prefix":"10.1016","volume":"248","author":[{"given":"Jadera","family":"Talap","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minzhe","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lushan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0876-0050","authenticated-orcid":false,"given":"Sheng","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.talanta.2022.123644_bib1","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41586-020-2012-7","article-title":"A pneumonia outbreak associated with a new coronavirus of probable bat origin","volume":"579","author":"Zhou","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.talanta.2022.123644_bib2","series-title":"WHO Coronavirus (COVID-19) Dashboard","year":"2021"},{"key":"10.1016\/j.talanta.2022.123644_bib3","doi-asserted-by":"crossref","first-page":"e20","DOI":"10.1016\/S2213-2600(21)00005-9","article-title":"New variant of SARS-CoV-2 in UK causes surge of COVID-19","volume":"9","author":"Kirby","year":"2021","journal-title":"Lancet Respir. Med."},{"key":"10.1016\/j.talanta.2022.123644_bib4","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1016\/S0140-6736(21)01358-1","article-title":"SARS-CoV-2 delta VOC in Scotland: demographics, risk of hospital admission, and vaccine effectiveness","volume":"397","author":"Sheikh","year":"2021","journal-title":"Lancet"},{"key":"10.1016\/j.talanta.2022.123644_bib5","first-page":"n1445","article-title":"COVID-19: delta variant is now UK's most dominant strain and spreading through schools","volume":"373","author":"Torjesen","year":"2021","journal-title":"BMJ Br. Med. J. (Clin. Res. Ed.)"},{"key":"10.1016\/j.talanta.2022.123644_bib6","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1038\/s41564-020-0695-z","article-title":"The species severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2","volume":"5","author":"Gorbalenya","year":"2020","journal-title":"Nat. Microbiol"},{"key":"10.1016\/j.talanta.2022.123644_bib7","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1002\/jmv.25681","article-title":"Emerging coronaviruses: genome structure, replication, and pathogenesis","volume":"92","author":"Chen","year":"2020","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.talanta.2022.123644_bib8","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.chom.2020.02.001","article-title":"Genome composition and divergence of the novel coronavirus (2019-nCoV) originating in China","volume":"27","author":"Wu","year":"2020","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.talanta.2022.123644_bib9","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1038\/s41401-020-0485-4","article-title":"Structural and functional properties of SARS-CoV-2 spike protein: potential antivirus drug development for COVID-19","volume":"41","author":"Huang","year":"2020","journal-title":"Acta Pharmacol. Sin."},{"key":"10.1016\/j.talanta.2022.123644_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.virusres.2021.198522","article-title":"Structural modelling of SARS-CoV-2 alpha variant (B.1.1.7) suggests enhanced furin binding and infectivity","volume":"303","author":"Mohammad","year":"2021","journal-title":"Virus Res."},{"key":"10.1016\/j.talanta.2022.123644_bib11","first-page":"103","article-title":"Structural consequences of variation in SARS-CoV-2 B.1.1.7","volume":"3","author":"Ostrov","year":"2021","journal-title":"J. Cell Immunol."},{"key":"10.1016\/j.talanta.2022.123644_bib12","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1093\/cid\/ciab636","article-title":"An update on SARS-CoV-2 diversity in the United States national capital region: evolution of novel and variants of concern","volume":"74","author":"Morris","year":"2021","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.talanta.2022.123644_bib13","article-title":"Delta spike P681R mutation enhances SARS-CoV-2 fitness over Alpha variant","author":"Liu","year":"2021","journal-title":"bioRxiv"},{"key":"10.1016\/j.talanta.2022.123644_bib14","doi-asserted-by":"crossref","first-page":"778","DOI":"10.3390\/clinpract11040093","article-title":"Analysis of the delta variant B.1.617.2 COVID-19","volume":"11","author":"Shiehzadegan","year":"2021","journal-title":"Clin. Pract."},{"key":"10.1016\/j.talanta.2022.123644_bib15","article-title":"The potential for SARS-CoV-2 to evade both natural and vaccine-induced immunity","author":"Shang","year":"2020","journal-title":"bioRxiv"},{"key":"10.1016\/j.talanta.2022.123644_bib16","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/s41586-020-2180-5","article-title":"Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor","volume":"581","author":"Lan","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.talanta.2022.123644_bib17","first-page":"1002","article-title":"Prime-boost vaccination of mice and rhesus macaques with two novel adenovirus vectored COVID-19 vaccine candidates, Emerg","volume":"10","author":"Luo","year":"2021","journal-title":"Microb. Infect."},{"key":"10.1016\/j.talanta.2022.123644_bib18","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1016\/j.biotechadv.2011.06.017","article-title":"Advances in microfluidic PCR for point-of-care infectious disease diagnostics","volume":"29","author":"Park","year":"2011","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.talanta.2022.123644_bib19","doi-asserted-by":"crossref","first-page":"3822","DOI":"10.1021\/acsnano.0c02624","article-title":"Diagnosing COVID-19: the disease and tools for detection","volume":"14","author":"Udugama","year":"2020","journal-title":"ACS Nano"},{"key":"10.1016\/j.talanta.2022.123644_bib20","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1038\/s41563-020-00906-z","article-title":"Diagnostics for SARS-CoV-2 infections","volume":"20","author":"Kevadiya","year":"2021","journal-title":"Nat. Mater."},{"key":"10.1016\/j.talanta.2022.123644_bib21","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.ijid.2021.04.018","article-title":"Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection","volume":"107","author":"Inaba","year":"2021","journal-title":"Int. J. Infect. Dis."},{"key":"10.1016\/j.talanta.2022.123644_bib22","doi-asserted-by":"crossref","first-page":"2089","DOI":"10.3389\/fmicb.2018.02089","article-title":"Comparative evaluation of the LAMP assay and PCR-based assays for the rapid detection of Alternaria solani","volume":"9","author":"Khan","year":"2018","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.talanta.2022.123644_bib23","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.jpha.2020.02.010","article-title":"Recent advances and perspectives of nucleic acid detection for coronavirus","volume":"10","author":"Shen","year":"2020","journal-title":"J. Pharm. Anal."},{"key":"10.1016\/j.talanta.2022.123644_bib24","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.talanta.2017.07.019","article-title":"Semi-quantitative visual detection of loop mediated isothermal amplification (LAMP)-generated DNA by distance-based measurement on a paper device","volume":"175","author":"Hongwarittorrn","year":"2017","journal-title":"Talanta"},{"key":"10.1016\/j.talanta.2022.123644_bib25","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/j.1574-695X.2011.00785.x","article-title":"Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications","volume":"62","author":"Francois","year":"2011","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.1016\/j.talanta.2022.123644_bib26","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.jbbm.2006.08.008","article-title":"Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances","volume":"70","author":"Kaneko","year":"2007","journal-title":"J. Biochem. Biophys. Methods"},{"key":"10.1016\/j.talanta.2022.123644_bib27","doi-asserted-by":"crossref","first-page":"3350","DOI":"10.1128\/JCM.00697-10","article-title":"Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of Taenia species from humans","volume":"48","author":"Nkouawa","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.talanta.2022.123644_bib28","doi-asserted-by":"crossref","first-page":"3242","DOI":"10.1021\/acssensors.1c00902","article-title":"Methylation-sensitive loop-mediated isothermal amplification (LAMP): nucleic acid methylation detection through LAMP with mobile fluorescence readout","volume":"6","author":"Hambalek","year":"2021","journal-title":"ACS Sens."},{"key":"10.1016\/j.talanta.2022.123644_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2021.122827","article-title":"A LAMP-based microfluidic module for rapid detection of pathogen in cryptococcal meningitis","volume":"236","author":"Tian","year":"2022","journal-title":"Talanta"},{"key":"10.1016\/j.talanta.2022.123644_bib30","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.bios.2014.05.039","article-title":"Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP)","volume":"61","author":"Zhang","year":"2014","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.talanta.2022.123644_bib31","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1007\/s10156-009-0669-9","article-title":"Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases","volume":"15","author":"Mori","year":"2009","journal-title":"J. Infect. Chemother."},{"key":"10.1016\/j.talanta.2022.123644_bib32","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.cell.2020.11.020","article-title":"Evaluating the effects of SARS-CoV-2 spike mutation D614G on transmissibility and pathogenicity","volume":"184","author":"Volz","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.talanta.2022.123644_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.meegid.2020.104445","article-title":"Characterizations of SARS-CoV-2 mutational profile, spike protein stability and viral transmission","volume":"85","author":"Laha","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.talanta.2022.123644_bib34","doi-asserted-by":"crossref","first-page":"6272","DOI":"10.1038\/s41467-020-20075-6","article-title":"Analytical validity of nanopore sequencing for rapid SARS-CoV-2 genome analysis","volume":"11","author":"Bull","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.talanta.2022.123644_bib35","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2021.26.3.2100008","article-title":"Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012\/01 and other variants with spike deletion H69-V70, France","volume":"26","author":"Bal","year":"2021","journal-title":"Euro Surveill."},{"key":"10.1016\/j.talanta.2022.123644_bib36","doi-asserted-by":"crossref","first-page":"e95","DOI":"10.1016\/j.dental.2021.01.015","article-title":"Fast and cost-effective screening for SARS-CoV-2 variants in a routine diagnostic setting","volume":"37","author":"Durner","year":"2021","journal-title":"Dent. Mater."},{"key":"10.1016\/j.talanta.2022.123644_bib37","article-title":"A simple RT-PCR melting temperature assay to rapidly screen for widely circulating SARS-CoV-2 variants","author":"Banada","year":"2021","journal-title":"medRxiv"},{"key":"10.1016\/j.talanta.2022.123644_bib38","doi-asserted-by":"crossref","first-page":"3393","DOI":"10.1021\/acs.analchem.0c04303","article-title":"Detection of SARS-CoV-2 and its mutated variants via CRISPR-Cas13-based transcription amplification","volume":"93","author":"Wang","year":"2021","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2022.123644_bib39","doi-asserted-by":"crossref","first-page":"3314","DOI":"10.1021\/ac504387c","article-title":"Robust strand exchange reactions for the sequence-specific, real-time detection of nucleic acid amplicons","volume":"87","author":"Jiang","year":"2015","journal-title":"Anal. Chem."}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914022004404?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914022004404?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T13:02:18Z","timestamp":1758546138000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914022004404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10]]},"references-count":39,"alternative-id":["S0039914022004404"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2022.123644","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2022,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"RT-LAMP assay combining multi-fluorescent probes for SARS-CoV-2 RNA detection and variant differentiation","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2022.123644","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"123644"}}