{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T19:53:52Z","timestamp":1793303632914,"version":"4.5.0"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022A1515010218"],"award-info":[{"award-number":["2022A1515010218"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010256","name":"Guangzhou Municipal Science and Technology Project","doi-asserted-by":"publisher","award":["201904010175"],"award-info":[{"award-number":["201904010175"]}],"id":[{"id":"10.13039\/501100010256","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010256","name":"Guangzhou Municipal Science and Technology Project","doi-asserted-by":"publisher","award":["202201011715"],"award-info":[{"award-number":["202201011715"]}],"id":[{"id":"10.13039\/501100010256","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82070589"],"award-info":[{"award-number":["82070589"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2024,1]]},"DOI":"10.1016\/j.talanta.2023.125106","type":"journal-article","created":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T02:19:54Z","timestamp":1692757194000},"page":"125106","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"P2","title":["Development of a sample preparation method for micro-proteomics analysis of the formaldehyde-fixed paraffin-embedded liver tissue samples"],"prefix":"10.1016","volume":"266","author":[{"given":"Yong-Er","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Lan","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-Tian","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Peng","family":"Zou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cui-Ting","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Min","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8526-2461","authenticated-orcid":false,"given":"Feng","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6851-3054","authenticated-orcid":false,"given":"Yan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.talanta.2023.125106_bib1","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1002\/1878-0261.12570","article-title":"High-throughput proteomic analysis of FFPE tissue samples facilitates tumor stratification","volume":"13","author":"Zhu","year":"2019","journal-title":"Mol. Oncol."},{"key":"10.1016\/j.talanta.2023.125106_bib2","doi-asserted-by":"crossref","first-page":"2956","DOI":"10.1038\/s41596-020-0356-y","article-title":"Spatially resolved analysis of FFPE tissue proteomes by quantitative mass spectrometry","volume":"15","author":"Buczak","year":"2020","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.talanta.2023.125106_bib3","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1074\/mcp.RA119.001889","article-title":"Quantitative profiling of the human substantia nigra proteome from laser-capture microdissected FFPE tissue","volume":"19","author":"Griesser","year":"2020","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.talanta.2023.125106_bib4","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12014-018-9188-y","article-title":"Reproducible proteomics sample preparation for single FFPE tissue slices using acid-labile surfactant and direct trypsinization","volume":"15","author":"Foll","year":"2018","journal-title":"Clin. Proteonomics"},{"key":"10.1016\/j.talanta.2023.125106_bib5","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1002\/path.5420","article-title":"A streamlined mass spectrometry-based proteomics workflow for large-scale FFPE tissue analysis","volume":"251","author":"Coscia","year":"2020","journal-title":"J. Pathol."},{"key":"10.1016\/j.talanta.2023.125106_bib6","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1038\/s41586-019-1173-8","article-title":"Proteomics reveals NNMT as a master metabolic regulator of cancer-associated fibroblasts","volume":"569","author":"Eckert","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.talanta.2023.125106_bib7","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1038\/s42255-022-00552-6","article-title":"Molecular consequences of SARS-CoV-2 liver tropism","volume":"4","author":"Wanner","year":"2022","journal-title":"Nat. Metab."},{"key":"10.1016\/j.talanta.2023.125106_bib8","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0142650","article-title":"Comparison of different buffers for protein extraction from formalin-fixed and paraffin-embedded tissue specimens","volume":"10","author":"Shen","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.talanta.2023.125106_bib9","doi-asserted-by":"crossref","first-page":"4217","DOI":"10.1021\/acs.jproteome.1c00321","article-title":"Proteomics for low cell numbers: how to optimize the sample preparation workflow for mass spectrometry analysis","volume":"20","author":"Kassem","year":"2021","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.talanta.2023.125106_bib10","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nmeth.1322","article-title":"Universal sample preparation method for proteome analysis","volume":"6","author":"Wisniewski","year":"2009","journal-title":"Nat. Methods"},{"key":"10.1016\/j.talanta.2023.125106_bib11","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/s41596-018-0082-x","article-title":"Single-pot, solid-phase-enhanced sample preparation for proteomics experiments","volume":"14","author":"Hughes","year":"2019","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.talanta.2023.125106_bib12","doi-asserted-by":"crossref","first-page":"1730","DOI":"10.1021\/acs.jproteome.7b00913","article-title":"Extending the compatibility of the SP3 paramagnetic bead processing approach for proteomics","volume":"17","author":"Moggridge","year":"2018","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.talanta.2023.125106_bib13","first-page":"757","volume":"10","author":"Hughes","year":"2014","journal-title":"Ultrasensitive proteome analysis using paramagnetic bead technology"},{"key":"10.1016\/j.talanta.2023.125106_bib14","doi-asserted-by":"crossref","first-page":"7113","DOI":"10.1038\/s41467-021-27398-y","article-title":"Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution","volume":"12","author":"Martinez-Val","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.talanta.2023.125106_bib15","doi-asserted-by":"crossref","first-page":"4060","DOI":"10.1021\/acs.jproteome.7b00433","article-title":"Evaluation of FASP, SP3, and iST protocols for proteomic sample preparation in the low microgram range","volume":"16","author":"Sielaff","year":"2017","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.talanta.2023.125106_bib16","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1080\/14789450.2021.1984886","article-title":"Laser Capture Proteomics: spatial tissue molecular profiling from the bench to personalized medicine","volume":"18","author":"Liotta","year":"2021","journal-title":"Expert Rev. Proteomics"},{"key":"10.1016\/j.talanta.2023.125106_bib17","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1038\/s41467-018-03367-w","article-title":"Nanodroplet processing platform for deep and quantitative proteome profiling of 10-100 mammalian cells","volume":"9","author":"Zhu","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.talanta.2023.125106_bib18","doi-asserted-by":"crossref","first-page":"5879","DOI":"10.1021\/acs.analchem.8b00596","article-title":"Spatial-resolution cell type proteome profiling of cancer tissue by fully integrated proteomics Technology","volume":"90","author":"Xu","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2023.125106_bib19","doi-asserted-by":"crossref","first-page":"5430","DOI":"10.1021\/acs.analchem.8b00661","article-title":"Nanoliter-Scale oil-air-Droplet chip-based single cell proteomic analysis","volume":"90","author":"Li","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2023.125106_bib20","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1038\/s41587-022-01302-5","article-title":"Deep Visual Proteomics defines single-cell identity and heterogeneity","volume":"40","author":"Mund","year":"2022","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.talanta.2023.125106_bib21","doi-asserted-by":"crossref","first-page":"2631","DOI":"10.1021\/acs.jproteome.9b00587","article-title":"Proteomics analysis of formalin fixed paraffin embedded tissues in the investigation of prostate cancer","volume":"19","author":"Mantsiou","year":"2020","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.talanta.2023.125106_bib22","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.1002\/hep.29336","article-title":"Argininosuccinate synthase 1 (ASS1): a marker of unclassified hepatocellular adenoma and high bleeding risk","volume":"66","author":"Henriet","year":"2017","journal-title":"Hepatology"},{"key":"10.1016\/j.talanta.2023.125106_bib23","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1002\/mas.21598","article-title":"Proteome analysis of tissues by mass spectrometry","volume":"38","author":"Dapic","year":"2019","journal-title":"Mass Spectrom. Rev."},{"key":"10.1016\/j.talanta.2023.125106_bib24","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","article-title":"Measurement of protein using bicinchoninic acid","volume":"150","author":"Smith","year":"1985","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.talanta.2023.125106_bib25","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.talanta.2023.125106_bib26","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.talanta.2023.125106_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2021.122568","article-title":"Comparison of different digestion methods for proteomic analysis of isolated cells and FFPE tissue samples","volume":"233","author":"Pirog","year":"2021","journal-title":"Talanta"},{"key":"10.1016\/j.talanta.2023.125106_bib28","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.202200085","article-title":"Comparison of matched formalin-fixed paraffin embedded and fresh frozen meningioma tissue reveals bias in proteomic profiles","volume":"22","author":"Schoffman","year":"2022","journal-title":"Proteomics"},{"key":"10.1016\/j.talanta.2023.125106_bib29","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1074\/mcp.T400003-MCP200","article-title":"Trypsin cleaves exclusively C-terminal to arginine and lysine residues","volume":"3","author":"Olsen","year":"2004","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.talanta.2023.125106_bib30","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.bbapap.2014.10.003","article-title":"Proteomic developments in the analysis of formalin-fixed tissue","volume":"1854","author":"Gustafsson","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.talanta.2023.125106_bib31","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1039\/C5MB00670H","article-title":"Comparison of multiple protein extraction buffers for GeLC-MS\/MS proteomic analysis of liver and colon formalin-fixed, paraffin-embedded tissues","volume":"12","author":"Broeckx","year":"2016","journal-title":"Mol. Biosyst."},{"key":"10.1016\/j.talanta.2023.125106_bib32","doi-asserted-by":"crossref","first-page":"9670","DOI":"10.1021\/ac5022216","article-title":"Workflow for combined proteomics and glycomics profiling from histological tissues","volume":"86","author":"Turiak","year":"2014","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2023.125106_bib33","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1074\/mcp.RA118.001267","article-title":"Quantitative microproteomics based characterization of the central and peripheral nervous system of a mouse model of krabbe Disease","volume":"18","author":"Pellegrini","year":"2019","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.talanta.2023.125106_bib34","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1038\/s41467-022-28524-0","article-title":"Proteomic analysis of archival breast cancer clinical specimens identifies biological subtypes with distinct survival outcomes","volume":"13","author":"Asleh","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.talanta.2023.125106_bib35","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1074\/mcp.TIR118.001270","article-title":"Protein aggregation capture on microparticles enables multipurpose proteomics sample preparation","volume":"18","author":"Batth","year":"2019","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.talanta.2023.125106_bib36","doi-asserted-by":"crossref","first-page":"2568","DOI":"10.1002\/pmic.201400454","article-title":"Unrestricted modification search reveals lysine methylation as major modification induced by tissue formalin fixation and paraffin embedding","volume":"15","author":"Zhang","year":"2015","journal-title":"Proteomics"},{"key":"10.1016\/j.talanta.2023.125106_bib37","doi-asserted-by":"crossref","DOI":"10.3389\/fmed.2021.657313","article-title":"Protein extraction from FFPE kidney tissue samples: a review of the literature and characterization of techniques","volume":"8","author":"Garcia-Vence","year":"2021","journal-title":"Front. Med."},{"key":"10.1016\/j.talanta.2023.125106_bib38","doi-asserted-by":"crossref","DOI":"10.3390\/molecules27031137","article-title":"Evaluation of fast and sensitive proteome profiling of FF and FFPE kidney patient tissues","volume":"27","author":"Dapic","year":"2022","journal-title":"Molecules"}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914023008573?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914023008573?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T13:15:22Z","timestamp":1758546922000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914023008573"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":38,"alternative-id":["S0039914023008573"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2023.125106","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a sample preparation method for micro-proteomics analysis of the formaldehyde-fixed paraffin-embedded liver tissue samples","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2023.125106","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"125106"}}