{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T11:41:32Z","timestamp":1771674092408,"version":"3.50.1"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2024,10]]},"DOI":"10.1016\/j.talanta.2024.126501","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T06:07:47Z","timestamp":1719814067000},"page":"126501","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["A precise microdissection strategy enabled spatial heterogeneity analysis on the targeted region of formalin-fixed paraffin-embedded tissues"],"prefix":"10.1016","volume":"278","author":[{"given":"Chen","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingjie","family":"Zou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Likun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingwen","family":"Shan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiping","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6675-4452","authenticated-orcid":false,"given":"Guohua","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"9","key":"10.1016\/j.talanta.2024.126501_bib1","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1038\/s12276-020-00499-2","article-title":"Single-cell sequencing techniques from individual to multiomics analyses","volume":"52","author":"Kashima","year":"2020","journal-title":"Exp. Mol. Med."},{"issue":"1","key":"10.1016\/j.talanta.2024.126501_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41592-019-0703-5","article-title":"Single-cell multimodal omics","volume":"17","year":"2020","journal-title":"Nat. Methods"},{"issue":"2","key":"10.1016\/j.talanta.2024.126501_bib3","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1038\/s42255-022-00531-x","article-title":"Single-cell multi-omics analysis of human pancreatic islets reveals novel cellular states in type 1 diabetes","volume":"4","author":"Fasolino","year":"2022","journal-title":"Nat. Metab."},{"issue":"8","key":"10.1016\/j.talanta.2024.126501_bib4","doi-asserted-by":"crossref","first-page":"16897","DOI":"10.3390\/ijms160816897","article-title":"Technologies for single-cell isolation","volume":"16","author":"Gross","year":"2015","journal-title":"Int. J. Mol. Sci."},{"issue":"6294","key":"10.1016\/j.talanta.2024.126501_bib5","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1126\/science.aaf2403","article-title":"Visualization and analysis of gene expression in tissue sections by spatial transcriptomics","volume":"353","author":"St\u00e5hl","year":"2016","journal-title":"Science"},{"issue":"7922","key":"10.1016\/j.talanta.2024.126501_bib6","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1038\/s41586-022-05023-2","article-title":"Spatially resolved clonal copy number alterations in benign and malignant tissue","volume":"608","author":"Erickson","year":"2022","journal-title":"Nature"},{"issue":"7894","key":"10.1016\/j.talanta.2024.126501_bib7","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1038\/d41586-022-00163-x","article-title":"Seven technologies to watch in 2022","volume":"601","author":"Eisenstein","year":"2022","journal-title":"Nature"},{"issue":"7","key":"10.1016\/j.talanta.2024.126501_bib8","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1038\/s41592-020-0858-0","article-title":"Highly specific multiplexed RNA imaging in tissues with split-FISH","volume":"17","author":"Goh","year":"2020","journal-title":"Nat. Methods"},{"issue":"2","key":"10.1016\/j.talanta.2024.126501_bib9","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abk0133","article-title":"Computer-aided design of reversible hybridization chain reaction (CAD-HCR) enables multiplexed single-cell spatial proteomics imaging","volume":"8","author":"Liu","year":"2022","journal-title":"Sci. Adv."},{"key":"10.1016\/j.talanta.2024.126501_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2021.113507","article-title":"Photothermal mediated rolling circle amplification toward specific and direct in situ mRNA detection","volume":"192","author":"Liu","year":"2021","journal-title":"Biosens. Bioelectron."},{"issue":"6","key":"10.1016\/j.talanta.2024.126501_bib11","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1038\/s41592-019-0404-0","article-title":"SABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and tissues","volume":"16","author":"Kishi","year":"2019","journal-title":"Nat. Methods"},{"issue":"1","key":"10.1016\/j.talanta.2024.126501_bib12","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1038\/s41467-021-21615-4","article-title":"Inter- and intra-tumor heterogeneity of metastatic prostate cancer determined by digital spatial gene expression profiling","volume":"12","author":"Brady","year":"2021","journal-title":"Nat. Commun."},{"issue":"5","key":"10.1016\/j.talanta.2024.126501_bib13","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1038\/s41587-020-0472-9","article-title":"Multiplex digital spatial profiling of proteins and RNA in fixed tissue","volume":"38","author":"Merritt","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.talanta.2024.126501_bib14","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms12139","article-title":"Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling","volume":"7","author":"Nichterwitz","year":"2016","journal-title":"Nat. Commun."},{"issue":"2","key":"10.1016\/j.talanta.2024.126501_bib15","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1002\/path.5575","article-title":"Whole-slide laser microdissection for tumour enrichment","volume":"253","author":"Coope","year":"2021","journal-title":"J. Pathol."},{"issue":"1","key":"10.1016\/j.talanta.2024.126501_bib16","doi-asserted-by":"crossref","first-page":"4325","DOI":"10.1038\/s41598-017-04616-6","article-title":"Site-specific gene expression analysis using an automated tissue micro-dissection punching system","volume":"7","author":"Yoda","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.talanta.2024.126501_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2020.121895","article-title":"A 3D hydrogel based on chitosan and carbon dots for sensitive fluorescence detection of microRNA-21 in breast cancer cells","volume":"224","author":"Mohammadi","year":"2021","journal-title":"Talanta"},{"issue":"23","key":"10.1016\/j.talanta.2024.126501_bib18","doi-asserted-by":"crossref","DOI":"10.1002\/adma.202006582","article-title":"Engineering hydrogel-based biomedical photonics: design, fabrication, and applications","volume":"33","author":"Guimar\u00e3es","year":"2021","journal-title":"Adv. Mater."},{"issue":"1","key":"10.1016\/j.talanta.2024.126501_bib19","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1038\/s41392-021-00830-x","article-title":"Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity","volume":"6","author":"Cao","year":"2021","journal-title":"Signal Transduct. Targeted Ther."},{"issue":"13","key":"10.1016\/j.talanta.2024.126501_bib20","doi-asserted-by":"crossref","first-page":"5529","DOI":"10.1021\/acs.analchem.1c00035","article-title":"Multiplex visualized closed-tube PCR with hamming distance 2 code for 15 HPV subtype typing","volume":"93","author":"Weng","year":"2021","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2024.126501_bib21","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.bios.2016.12.015","article-title":"Visualized detection of single-base difference in multiplexed loop-mediated isothermal amplification amplicons by invasive reaction coupled with oligonucleotide probe-modified gold nanoparticles","volume":"90","author":"Lu","year":"2017","journal-title":"Biosens. Bioelectron."},{"issue":"5","key":"10.1016\/j.talanta.2024.126501_bib22","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1016\/j.ccell.2018.03.017","article-title":"Multi-stage differentiation defines melanoma subtypes with differential vulnerability to drug-induced iron-dependent oxidative stress","volume":"33","author":"Tsoi","year":"2018","journal-title":"Cancer Cell"},{"issue":"1","key":"10.1016\/j.talanta.2024.126501_bib23","doi-asserted-by":"crossref","first-page":"5499","DOI":"10.1038\/s41467-019-13329-5","article-title":"An independent poor-prognosis subtype of breast cancer defined by a distinct tumor immune microenvironment","volume":"10","author":"Tekpli","year":"2019","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.talanta.2024.126501_bib24","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1038\/s41467-020-15283-z","article-title":"Quantitative proteomic landscape of metaplastic breast carcinoma pathological subtypes and their relationship to triple-negative tumors","volume":"11","author":"Djomehri","year":"2020","journal-title":"Nat. Commun."},{"issue":"5","key":"10.1016\/j.talanta.2024.126501_bib25","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1038\/s41571-020-00462-0","article-title":"Broadening horizons: the role of ferroptosis in cancer","volume":"18","author":"Chen","year":"2021","journal-title":"Nat. Rev. Clin. Oncol."},{"issue":"7","key":"10.1016\/j.talanta.2024.126501_bib26","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1038\/s41568-022-00459-0","article-title":"Targeting ferroptosis as a vulnerability in cancer","volume":"22","author":"Lei","year":"2022","journal-title":"Nat. Rev. Cancer"},{"issue":"1","key":"10.1016\/j.talanta.2024.126501_bib27","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/s12943-020-01168-8","article-title":"CAF secreted miR-522 suppresses ferroptosis and promotes acquired chemo-resistance in gastric cancer","volume":"19","author":"Zhang","year":"2020","journal-title":"Mol. Cancer"},{"key":"10.1016\/j.talanta.2024.126501_bib28","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.omtn.2022.02.013","article-title":"Exosomal microRNAs synergistically trigger stromal fibroblasts in breast cancer","volume":"28","author":"Scognamiglio","year":"2022","journal-title":"Mol. Ther. Nucleic Acids"},{"issue":"3","key":"10.1016\/j.talanta.2024.126501_bib29","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.ccell.2018.01.011","article-title":"Fibroblast heterogeneity and immunosuppressive environment in human breast cancer","volume":"33","author":"Costa","year":"2018","journal-title":"Cancer Cell"},{"issue":"18","key":"10.1016\/j.talanta.2024.126501_bib30","doi-asserted-by":"crossref","first-page":"7086","DOI":"10.1021\/acs.analchem.1c00564","article-title":"Programmable one-pot enzymatic reaction for direct fluorescence detection of ultralow-abundance mutations in the DNA duplex","volume":"93","author":"Yang","year":"2021","journal-title":"Anal. Chem."},{"issue":"26","key":"10.1016\/j.talanta.2024.126501_bib31","doi-asserted-by":"crossref","first-page":"6751","DOI":"10.1007\/s00216-018-1316-z","article-title":"Highly sensitive and specific real-time PCR by employing serial invasive reaction as a sequence identifier for quantifying EGFR mutation abundance in cfDNA","volume":"410","author":"Xiang","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"issue":"1","key":"10.1016\/j.talanta.2024.126501_bib32","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1021\/acs.analchem.3c03326","article-title":"Ultrafast and highly specific detection of one-base mutated cell-free DNA at a very low abundance","volume":"96","author":"Ma","year":"2024","journal-title":"Anal. Chem."},{"issue":"2","key":"10.1016\/j.talanta.2024.126501_bib33","article-title":"Diagnostic, prognostic, and therapeutic significance of long non-coding RNA MALAT1 in cancer","volume":"1875","author":"Goyal","year":"2021","journal-title":"Biochim. Biophys. Acta Rev. Canc"}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914024008804?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914024008804?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,11,23]],"date-time":"2024-11-23T04:16:12Z","timestamp":1732335372000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914024008804"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":33,"alternative-id":["S0039914024008804"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2024.126501","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A precise microdissection strategy enabled spatial heterogeneity analysis on the targeted region of formalin-fixed paraffin-embedded tissues","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2024.126501","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"126501"}}