{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T15:43:28Z","timestamp":1769960608645,"version":"3.49.0"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100012905","name":"Department of Science and Technology of Shandong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012905","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2020MH324"],"award-info":[{"award-number":["ZR2020MH324"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2020QH271"],"award-info":[{"award-number":["ZR2020QH271"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2024,10]]},"DOI":"10.1016\/j.talanta.2024.126538","type":"journal-article","created":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T21:37:00Z","timestamp":1720906620000},"page":"126538","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Graphene oxide-functionalized molecular beacon for real-time interference-free detection of Ki-67 mRNA in living cells"],"prefix":"10.1016","volume":"278","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8573-8690","authenticated-orcid":false,"given":"Qian","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6534-882X","authenticated-orcid":false,"given":"Chihao","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongming","family":"Xing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.talanta.2024.126538_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2020.116143","article-title":"Molecular beacon strategies for sensing purpose","volume":"134","author":"Bidar","year":"2021","journal-title":"Trac-Trend Anal Chem"},{"issue":"9","key":"10.1016\/j.talanta.2024.126538_bib2","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1038\/s41551-022-00932-z","article-title":"Spatially resolved in vivo imaging of inflammation-associated mRNA via enzymatic fluorescence amplification in a molecular beacon","volume":"6","author":"Sheng","year":"2022","journal-title":"Nat. Biomed. Eng."},{"issue":"5","key":"10.1016\/j.talanta.2024.126538_bib3","doi-asserted-by":"crossref","first-page":"2399","DOI":"10.1021\/acs.analchem.1c03950","article-title":"In situ detection of nanotoxicity in living cells based on multiple miRNAs probed by a peptide functionalized nanoprobe","volume":"94","author":"Han","year":"2022","journal-title":"Anal. Chem."},{"issue":"9","key":"10.1016\/j.talanta.2024.126538_bib4","doi-asserted-by":"crossref","first-page":"3678","DOI":"10.1021\/acs.nanolett.2c04643","article-title":"A multiple targeting nanoprobe for identifying cancer metastatic sites based on detection of various mRNAs in circulating tumor cells","volume":"23","author":"Han","year":"2023","journal-title":"Nano Lett."},{"issue":"45","key":"10.1016\/j.talanta.2024.126538_bib5","doi-asserted-by":"crossref","first-page":"17522","DOI":"10.1073\/pnas.0807066105","article-title":"Visualizing the dynamics of viral replication in living cells via Tat peptide delivery of nuclease-resistant molecular beacons","volume":"105","author":"Yeh","year":"2008","journal-title":"P Natl Acad Sci USA"},{"key":"10.1016\/j.talanta.2024.126538_bib6","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.biomaterials.2016.05.022","article-title":"Single-molecule detection and tracking of RNA transcripts in living cells using phosphorothioate-optimized 20-O-methyl RNA molecular beacons","volume":"100","author":"Zhao","year":"2016","journal-title":"Biomaterials"},{"issue":"13","key":"10.1016\/j.talanta.2024.126538_bib7","doi-asserted-by":"crossref","first-page":"5437","DOI":"10.1021\/acs.analchem.0c05060","article-title":"Rational design of a dual-layered metal\u2013organic framework nanostructure for enhancing the cell imaging of molecular beacons","volume":"93","author":"Gao","year":"2021","journal-title":"Anal. Chem."},{"issue":"8","key":"10.1016\/j.talanta.2024.126538_bib8","doi-asserted-by":"crossref","first-page":"2378","DOI":"10.1021\/acssensors.0c00212","article-title":"Rigidified DNA triangle-protected molecular beacon from endogenous nuclease digestion for monitoring microRNA expression in living cells","volume":"5","author":"Li","year":"2020","journal-title":"ACS Sens."},{"issue":"10","key":"10.1016\/j.talanta.2024.126538_bib9","doi-asserted-by":"crossref","first-page":"7082","DOI":"10.1039\/C7SC02819A","article-title":"Fluorinated molecular beacons as functional DNA nanomolecules for cellular imaging","volume":"8","author":"Jin","year":"2017","journal-title":"Chem. Sci."},{"key":"10.1016\/j.talanta.2024.126538_bib10","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.gde.2019.07.012","article-title":"Wherever I may roam: organellar protein targeting and evolvability","volume":"58\u201359","author":"Dunn","year":"2019","journal-title":"Curr. Opin. Genet. Dev."},{"issue":"44","key":"10.1016\/j.talanta.2024.126538_bib11","doi-asserted-by":"crossref","first-page":"10442","DOI":"10.1039\/c3sm50722j","article-title":"Physical modelling of the nuclear pore complex","volume":"9","author":"Osmanovi\u0107","year":"2013","journal-title":"Soft Matter"},{"issue":"6","key":"10.1016\/j.talanta.2024.126538_bib12","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.1021\/ja311080j","article-title":"Nanoscale mechanism of molecular transport through the nuclear pore complex as studied by scanning electrochemical microscopy","volume":"135","author":"Kim","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.talanta.2024.126538_bib13","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.snb.2018.12.025","article-title":"A smart approach toward rhodamine spiro-ring derivatives sensing platform for lysosome-targetable imaging applications","volume":"283","author":"Gong","year":"2019","journal-title":"Sensor Actuat B- Chem"},{"issue":"37","key":"10.1016\/j.talanta.2024.126538_bib14","doi-asserted-by":"crossref","first-page":"8974","DOI":"10.1039\/D3TB01311A","article-title":"An ultra pH-responsive peptide nanocarrier for cancer gene therapy","volume":"11","author":"Wang","year":"2023","journal-title":"J. Mater. Chem. B"},{"issue":"6","key":"10.1016\/j.talanta.2024.126538_bib15","doi-asserted-by":"crossref","first-page":"3736","DOI":"10.1021\/jacs.2c13222","article-title":"Molecular engineering of pH-responsive NIR oxazine assemblies for evoking tumor ferroptosis via triggering lysosomal dysfunction","volume":"145","author":"Li","year":"2023","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.talanta.2024.126538_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2023.134809","article-title":"A buffering fluorogenic probe for real-time lysosomal pH monitoring","volume":"399","author":"Zhang","year":"2024","journal-title":"Sensor Actuat B- Chem"},{"key":"10.1016\/j.talanta.2024.126538_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2021.130350","article-title":"A ratiometric fluorogenic nanoprobe for real-time quantitative monitoring of lysosomal pH","volume":"345","author":"Zhang","year":"2021","journal-title":"Sensor Actuat B- Chem"},{"issue":"26","key":"10.1016\/j.talanta.2024.126538_bib18","doi-asserted-by":"crossref","DOI":"10.1002\/adfm.202214655","article-title":"Nanomaterials responsive to endogenous biomarkers for cardiovascular disease theranostics","volume":"33","author":"Wei","year":"2023","journal-title":"Adv. Funct. Mater."},{"issue":"9","key":"10.1016\/j.talanta.2024.126538_bib19","doi-asserted-by":"crossref","DOI":"10.1002\/mabi.202100183","article-title":"A review of pH-responsive organic\u2013inorganic hybrid nanoparticles for RNAi-based therapeutics","volume":"21","author":"Wang","year":"2021","journal-title":"Macromol. Biosci."},{"key":"10.1016\/j.talanta.2024.126538_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2023.134429","article-title":"ATP and ssDNA aptamer-mediated peroxidase-like activity of rGO@PDA@CeO2 nanozyme: exosomal proteins profiling and detection at physiological pH for colorimetric sensor","volume":"394","author":"Kuang","year":"2023","journal-title":"Sensor Actuat B- Chem"},{"key":"10.1016\/j.talanta.2024.126538_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2023.123432","article-title":"Yeast \u03b2-D-glucan functionalized graphene oxide for macrophage-targeted delivery of CpG oligodeoxynucleotides and synergistically enhanced antitumor immunity","volume":"234","author":"Cheng","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"issue":"16","key":"10.1016\/j.talanta.2024.126538_bib22","doi-asserted-by":"crossref","first-page":"7987","DOI":"10.1021\/ac401845p","article-title":"Mechanisms of DNA sensing on graphene oxide","volume":"85","author":"Liu","year":"2013","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2024.126538_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.colsurfa.2022.128864","article-title":"Effect of pH on caffeine removal from aqueous media by graphene\/graphene oxide adsorption","volume":"644","author":"Wang","year":"2022","journal-title":"Colloid. Surface."},{"key":"10.1016\/j.talanta.2024.126538_bib24","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.cej.2019.01.027","article-title":"Planarity effect of polychlorinated biphenyls adsorption by graphene nanomaterials: the influence of graphene characteristics, solution pH and temperature","volume":"362","author":"Ren","year":"2019","journal-title":"Chem Eng J"},{"issue":"11","key":"10.1016\/j.talanta.2024.126538_bib25","doi-asserted-by":"crossref","DOI":"10.3390\/nano12111801","article-title":"Defect density-dependent pH response of graphene derivatives: towards the development of pH-sensitive graphene oxide devices","volume":"12","author":"Angizi","year":"2022","journal-title":"Nanomaterials"},{"issue":"19","key":"10.1016\/j.talanta.2024.126538_bib26","doi-asserted-by":"crossref","first-page":"10601","DOI":"10.1021\/acs.analchem.7b03146","article-title":"Visualizing breast cancer cell proliferation and invasion for assessing drug efficacy with a fluorescent nanoprobe","volume":"89","author":"Luan","year":"2017","journal-title":"Anal. Chem."},{"issue":"8","key":"10.1016\/j.talanta.2024.126538_bib27","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1007\/s00604-019-3685-1","article-title":"Effects of free patchy ends in ssDNA and dsDNA on gold nanoparticles in a colorimetric gene sensor for Hepatitis C virus RNA","volume":"186","author":"Mohammed","year":"2019","journal-title":"Microchim. Acta"},{"key":"10.1016\/j.talanta.2024.126538_bib28","doi-asserted-by":"crossref","first-page":"5853","DOI":"10.1038\/ncomms6853","article-title":"DNase II-dependent DNA digestion is required for DNA sensing by TLR9","volume":"6","author":"Chan","year":"2015","journal-title":"Nat. Commun."},{"key":"10.1016\/j.talanta.2024.126538_bib29","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.carbpol.2017.03.090","article-title":"Simply constructed chitosan nanocarriers with precise spatiotemporal control for efficient intracellular drug delivery","volume":"169","author":"Kong","year":"2017","journal-title":"Carbohyd Polym"},{"issue":"12","key":"10.1016\/j.talanta.2024.126538_bib30","doi-asserted-by":"crossref","first-page":"5461","DOI":"10.1021\/jacs.1c13426","article-title":"Acid-resistant and physiological pH-responsive DNA hydrogel composed of A-Motif and i-Motif toward oral insulin delivery","volume":"144","author":"Hu","year":"2022","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.talanta.2024.126538_bib31","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.flatc.2017.02.001","article-title":"Theoretical study on the interaction of nucleotides on two-dimensional atomically thin graphene and molybdenum disulfide","volume":"2","author":"Lianga","year":"2017","journal-title":"FlatChem"},{"issue":"8","key":"10.1016\/j.talanta.2024.126538_bib32","doi-asserted-by":"crossref","first-page":"6982","DOI":"10.1021\/acsami.7b19284","article-title":"A highly sensitive strategy for fluorescence imaging of microRNA in living cells and in vivo based on graphene oxide-enhanced signal molecules quenching of molecular beacon","volume":"10","author":"Yang","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.talanta.2024.126538_bib33","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.snb.2016.04.176","article-title":"Sensing of survivin mRNA in malignant astrocytes using graphene oxide nanocarrier-supported oligonucleotide molecular beacons","volume":"235","author":"Stobiecka","year":"2016","journal-title":"Sensor Actuat B-Chem"},{"issue":"9","key":"10.1016\/j.talanta.2024.126538_bib34","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.5012\/bkcs.2014.35.9.2749","article-title":"Graphene oxide-based direct measurement of DNase I activity with single stranded DNA","volume":"35","author":"Gang","year":"2014","journal-title":"B Korean Chem Soc"},{"issue":"1","key":"10.1016\/j.talanta.2024.126538_bib35","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1189\/jlb.4MR0316-108R","article-title":"Emerging roles of the processing of nucleic acids and Toll-like receptors in innate immune responses to nucleic acids","volume":"101","author":"Miyake","year":"2017","journal-title":"J Leukocyte Biol"},{"issue":"1","key":"10.1016\/j.talanta.2024.126538_bib36","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/s12967-023-03950-w","article-title":"An initial study on the predictive value using multiple MRI characteristics for Ki-67 labeling index in glioma","volume":"21","author":"Du","year":"2023","journal-title":"J. Transl. Med."},{"issue":"3","key":"10.1016\/j.talanta.2024.126538_bib37","doi-asserted-by":"crossref","first-page":"808","DOI":"10.3390\/cancers15030808","article-title":"The evolution of Ki-67 and breast carcinoma: past observations, present directions, and future considerations","volume":"15","author":"Finkelman","year":"2023","journal-title":"Cancers"},{"issue":"1","key":"10.1016\/j.talanta.2024.126538_bib38","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.canlet.2003.10.032","article-title":"Ki-67 and CEA expression as prognostic markers in Dukes' C colorectal cancer","volume":"207","author":"Ishida","year":"2004","journal-title":"Cancer Lett."},{"issue":"5","key":"10.1016\/j.talanta.2024.126538_bib39","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1016\/j.ijrobp.2008.12.061","article-title":"Evaluation of Ki-67 staining levels as an independent biomarker of biochemical recurrence after salvage radiation therapy for prostate cancer","volume":"75","author":"Parker","year":"2009","journal-title":"Int J Radiat Oncol"},{"issue":"5","key":"10.1016\/j.talanta.2024.126538_bib40","doi-asserted-by":"crossref","first-page":"2074","DOI":"10.1159\/000492545","article-title":"Mir-758-5p suppresses glioblastoma proliferation, migration and invasion by targeting ZBTB20","volume":"48","author":"Liu","year":"2018","journal-title":"Cell. Physiol. Biochem."},{"key":"10.1016\/j.talanta.2024.126538_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.139229","article-title":"Graphene nanosheets damage the lysosomal and mitochondrial membranes and induce the apoptosis of RBL-2H3 cells","volume":"734","author":"Liu","year":"2020","journal-title":"Sci. Total Environ."},{"issue":"7","key":"10.1016\/j.talanta.2024.126538_bib42","doi-asserted-by":"crossref","first-page":"510","DOI":"10.3390\/nano8070510","article-title":"Optical biosensing system for the detection of survivin mRNA in colorectal cancer cells using a graphene oxide carrier-bound oligonucleotide molecular beacon","volume":"8","author":"Katarzyna","year":"2018","journal-title":"Nanomaterials"},{"key":"10.1016\/j.talanta.2024.126538_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.colsurfb.2022.112998","article-title":"Graphene oxide internalization into mammalian cells - a review","volume":"221","author":"D\u0105browski","year":"2023","journal-title":"Colloid Surface B"},{"issue":"4","key":"10.1016\/j.talanta.2024.126538_bib44","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1517\/17425247.2011.561313","article-title":"Cellular delivery of siRNA and antisense oligonucleotides via receptor-mediated endocytosis","volume":"8","author":"Ming","year":"2011","journal-title":"Expert Opin Drug Deliv"}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914024009172?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914024009172?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T09:22:55Z","timestamp":1722676975000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914024009172"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":44,"alternative-id":["S0039914024009172"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2024.126538","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Graphene oxide-functionalized molecular beacon for real-time interference-free detection of Ki-67 mRNA in living cells","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2024.126538","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"126538"}}