{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T16:52:24Z","timestamp":1777654344782,"version":"3.51.4"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21735003"],"award-info":[{"award-number":["21735003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22204092"],"award-info":[{"award-number":["22204092"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2025,1]]},"DOI":"10.1016\/j.talanta.2024.127009","type":"journal-article","created":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T11:53:20Z","timestamp":1728474800000},"page":"127009","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Construction of a chemiluminescent biosensor based on enzymatic extension and click chemistry for sensitive measurement of MGMT activity in human breast tissues"],"prefix":"10.1016","volume":"282","author":[{"given":"Ting-ting","family":"Pan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huimin","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi-yue","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mao-tao","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-yang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"5955","key":"10.1016\/j.talanta.2024.127009_bib1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/308201a0","article-title":"Mechanism of mutagenesis by O6-methylguanine","volume":"308","author":"Eadie","year":"1984","journal-title":"Nature"},{"issue":"8839","key":"10.1016\/j.talanta.2024.127009_bib2","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/0140-6736(93)90069-S","article-title":"Deficient repair of DNA lesion O6-methylguanine in cirrhosis","volume":"341","author":"Collier","year":"1993","journal-title":"Lancet"},{"issue":"15","key":"10.1016\/j.talanta.2024.127009_bib3","doi-asserted-by":"crossref","first-page":"5709","DOI":"10.1021\/ja500172d","article-title":"Metal-dependent conformational activation explains highly promutagenic replication across O6-methylguanine by human DNA polymerase \u03b2","volume":"136","author":"Koag","year":"2014","journal-title":"J. Am. Chem. Soc."},{"issue":"20","key":"10.1016\/j.talanta.2024.127009_bib4","first-page":"5815","article-title":"Apoptosis induced by DNA damage O6-methylguanine is Bcl-2 and caspase-9\/3 regulated and Fas\/caspase-8 independent","volume":"60","author":"Ochs","year":"2000","journal-title":"Cancer Res."},{"issue":"1","key":"10.1016\/j.talanta.2024.127009_bib5","doi-asserted-by":"crossref","first-page":"3883","DOI":"10.1038\/s41467-020-17717-0","article-title":"MGMT genomic rearrangements contribute to chemotherapy resistance in gliomas","volume":"11","author":"Oldrini","year":"2020","journal-title":"Nat. Commun."},{"issue":"9","key":"10.1016\/j.talanta.2024.127009_bib6","doi-asserted-by":"crossref","first-page":"197","DOI":"10.31083\/j.fbl2809197","article-title":"Implications of advances in studies of O6-methylguanine-DNA- methyltransferase for tumor prognosis and treatment","volume":"28","author":"Chen","year":"2023","journal-title":"Front Biosci (Landmark Ed)"},{"issue":"4","key":"10.1016\/j.talanta.2024.127009_bib7","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1002\/ijc.10852","article-title":"Inactivation of the DNA repair gene O6-methylguanine-DNA methyltransferase by promoter hypermethylation and its relationship to aflatoxin B1-DNA adducts and p53 mutation in hepatocellular carcinoma","volume":"103","author":"Zhang","year":"2003","journal-title":"Int. J. Cancer"},{"issue":"14","key":"10.1016\/j.talanta.2024.127009_bib8","doi-asserted-by":"crossref","first-page":"2733","DOI":"10.1002\/1878-0261.13256","article-title":"MGMT inactivation as a new biomarker in patients with advanced biliary tract cancers","volume":"16","author":"Niger","year":"2022","journal-title":"Mol. Oncol."},{"issue":"Supplement","key":"10.1016\/j.talanta.2024.127009_bib9","first-page":"C233","article-title":"O6-methylguanine-DNA methyltransferase gene promoter methylation and lung cancer risk: a meta-analysis","volume":"12","author":"Yang","year":"2016","journal-title":"J. Cancer Res. Ther."},{"issue":"3","key":"10.1016\/j.talanta.2024.127009_bib10","doi-asserted-by":"crossref","first-page":"664","DOI":"10.4251\/wjgo.v14.i3.664","article-title":"O6-methylguanine DNA methyltransferase is upregulated in malignant transformation of gastric epithelial cells via its gene promoter DNA hypomethylation","volume":"14","author":"Chen","year":"2022","journal-title":"World J. Gastrointest. Oncol."},{"issue":"5","key":"10.1016\/j.talanta.2024.127009_bib11","first-page":"1125","article-title":"O6-methylguanine-DNA methyl transferase gene expression and prognosis in breast carcinoma","volume":"21","author":"Cayre","year":"2002","journal-title":"Int. J. Oncol."},{"issue":"62","key":"10.1016\/j.talanta.2024.127009_bib12","doi-asserted-by":"crossref","first-page":"8662","DOI":"10.1039\/D2CC03084E","article-title":"A simple and rapid mix-and-read assay for sensitive detection of O6-methylguanine DNA methyltransferase","volume":"58","author":"Liu","year":"2022","journal-title":"Chem. Commun."},{"issue":"10","key":"10.1016\/j.talanta.2024.127009_bib13","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1007\/s10434-001-0807-9","article-title":"Expression and prognostic significance of O6-methylguanine-DNA methyltransferase in hepatocellular, gastric, and breast cancers","volume":"8","author":"Matsukura","year":"2001","journal-title":"Ann. Surg Oncol."},{"issue":"12","key":"10.1016\/j.talanta.2024.127009_bib14","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1097\/00043426-200312000-00007","article-title":"O6-methylguanine-DNA methyltransferase activity and promoter methylation status in pediatric rhabdomyosarcoma","volume":"25","author":"Yeager","year":"2003","journal-title":"J. Pediatr. Hematol. Oncol."},{"issue":"1","key":"10.1016\/j.talanta.2024.127009_bib15","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.ab.2008.08.009","article-title":"Development of an O6-alkylguanine-DNA alkyltransferase assay based on covalent transfer of the benzyl moiety from [benzene-3H] O6-benzylguanine to the protein","volume":"383","author":"Ishiguro","year":"2008","journal-title":"Anal. Biochem."},{"issue":"4","key":"10.1016\/j.talanta.2024.127009_bib16","first-page":"279","article-title":"Importance of the DNA repair enzyme O6-alkyl guanine alkyltransferase (AT) in cancer chemotherapy, Cancer Treat","volume":"15","author":"D'Incalci","year":"1988","journal-title":"Rev."},{"key":"10.1016\/j.talanta.2024.127009_bib17","doi-asserted-by":"crossref","DOI":"10.4061\/2010\/632041","article-title":"Development of a novel fluorescence assay based on the use of the thrombin-binding aptamer for the detection of O6-alkylguanine-DNA alkyltransferase activity","volume":"2010","author":"Tintore","year":"2010","journal-title":"J. Nucleic Acids"},{"issue":"4","key":"10.1016\/j.talanta.2024.127009_bib18","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1111\/j.1750-3639.2008.00153.x","article-title":"Anti-O6-methylguanine-methyltransferase (MGMT) immunohistochemistry in glioblastoma multiforme: observer variability and lack of association with patient survival impede its use as clinical biomarker","volume":"18","author":"Preusser","year":"2008","journal-title":"Brain Pathol."},{"issue":"14","key":"10.1016\/j.talanta.2024.127009_bib19","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1039\/D4OB00120F","article-title":"A chloromethyl-triazole fluorescent chemosensor for O6-methylguanine DNA methyltransferase","volume":"22","author":"Ayan","year":"2024","journal-title":"Org. Biomol. Chem."},{"issue":"19","key":"10.1016\/j.talanta.2024.127009_bib20","doi-asserted-by":"crossref","first-page":"28195","DOI":"10.18632\/oncotarget.8618","article-title":"miRNA array screening reveals cooperative MGMT-regulation between miR-181d-5p and miR-409-3p in glioblastoma","volume":"7","author":"Khalil","year":"2016","journal-title":"Oncotarget"},{"issue":"36","key":"10.1016\/j.talanta.2024.127009_bib21","doi-asserted-by":"crossref","first-page":"19889","DOI":"10.1002\/anie.202106557","article-title":"\"Repaired and activated\" DNAzyme enables the monitoring of DNA alkylation repair in live cells","volume":"60","author":"Wang","year":"2021","journal-title":"Angew. Chem. Int. Ed."},{"issue":"11","key":"10.1016\/j.talanta.2024.127009_bib22","doi-asserted-by":"crossref","first-page":"4487","DOI":"10.1021\/acs.analchem.3c05090","article-title":"Integration of demethylation-activated DNAzyme with a single quantum dot nanosensor for sensitive detection of O6-methylguanine DNA methyltransferase in breast tissues","volume":"96","author":"Han","year":"2024","journal-title":"Anal. Chem"},{"issue":"9","key":"10.1016\/j.talanta.2024.127009_bib23","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1039\/D2OB00208F","article-title":"Harnessing an emissive guanine surrogate to design small-molecule fluorescent chemosensors of O6-methylguanine-DNA-methyltransferase (MGMT)","volume":"20","author":"Fillion","year":"2022","journal-title":"Org. Biomol. Chem."},{"issue":"7","key":"10.1016\/j.talanta.2024.127009_bib24","doi-asserted-by":"crossref","first-page":"969","DOI":"10.7150\/thno.14856","article-title":"Click chemistry-mediated nanosensors for biochemical assays","volume":"6","author":"Chen","year":"2016","journal-title":"Theranostics"},{"issue":"Pt 1","key":"10.1016\/j.talanta.2024.127009_bib25","article-title":"Enhanced imaging of protein-specific palmitoylation with HCR-based cis-membrane multi-FRET","volume":"266","author":"Fu","year":"2024","journal-title":"Talanta"},{"issue":"6","key":"10.1016\/j.talanta.2024.127009_bib26","doi-asserted-by":"crossref","first-page":"2623","DOI":"10.1093\/nar\/gkr1112","article-title":"A novel zinc-finger nuclease platform with a sequence-specific cleavage module","volume":"40","author":"Schierling","year":"2012","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"10.1016\/j.talanta.2024.127009_bib27","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1039\/C5SC02808F","article-title":"Protein sensing in living cells by molecular rotor-based fluorescence-switchable chemical probes","volume":"7","author":"Yu","year":"2016","journal-title":"Chem. Sci."},{"issue":"5","key":"10.1016\/j.talanta.2024.127009_bib28","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1007\/s00604-020-04280-0","article-title":"Fluorescence determination of the activity of O6-methylguanine-DNA methyltransferase based on the activation of restriction endonuclease and the use of graphene oxide","volume":"187","author":"Le","year":"2020","journal-title":"Mikrochim. Acta"},{"key":"10.1016\/j.talanta.2024.127009_bib29","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.aca.2013.10.036","article-title":"Target-mediated consecutive endonuclease reactions for specific and sensitive homogeneous fluorescence assay of O6-methylguanine-DNA methyltransferase","volume":"804","author":"Le","year":"2013","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2024.127009_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2023.341208","article-title":"RNA demethylation-driven functional supramolecular structure for label-free detection of m6A modification eraser FTO in human breast tissues","volume":"1260","author":"Li","year":"2023","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2024.127009_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2019.120317","article-title":"Latest developments in the detection and separation of bovine serum albumin using molecularly imprinted polymers","volume":"207","author":"Jahanban-Esfahlan","year":"2020","journal-title":"Talanta"},{"issue":"9","key":"10.1016\/j.talanta.2024.127009_bib32","doi-asserted-by":"crossref","first-page":"3649","DOI":"10.1073\/pnas.71.9.3649","article-title":"An N-glycosidase from escherichia coli that releases free uracil from DNA containing deaminated cytosine residues","volume":"71","author":"Lindahl","year":"1974","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.talanta.2024.127009_bib33","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.talanta.2018.09.113","article-title":"Multiple sealed primers-mediated rolling circle amplification strategy for sensitive and specific detection of DNA methyltransferase activity","volume":"194","author":"Xu","year":"2019","journal-title":"Talanta"},{"issue":"3","key":"10.1016\/j.talanta.2024.127009_bib34","doi-asserted-by":"crossref","first-page":"102","DOI":"10.3390\/bios9030102","article-title":"Overview of optical and electrochemical alkaline phosphatase (ALP) biosensors: recent approaches in cells culture techniques","volume":"9","author":"Balbaied","year":"2019","journal-title":"Biosensors"},{"issue":"4","key":"10.1016\/j.talanta.2024.127009_bib35","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.ctrv.2006.03.004","article-title":"Inactivation of O6-alkylguanine DNA alkyltransferase as a means to enhance chemotherapy","volume":"32","author":"Rabik","year":"2006","journal-title":"Cancer Treat Rev."},{"issue":"3","key":"10.1016\/j.talanta.2024.127009_bib36","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1002\/ijc.1336","article-title":"Inactivation of O6-methylguanine-DNA methyltransferase by glucose-conjugated inhibitors","volume":"93","author":"Reinhard","year":"2001","journal-title":"Int. J. Cancer"},{"issue":"3","key":"10.1016\/j.talanta.2024.127009_bib37","first-page":"121","article-title":"Inverse correlation between the expression of O6-methylguanine-DNA methyl transferase (MGMT) and p53 in breast cancer, Jpn","volume":"35","author":"Osanai","year":"2005","journal-title":"J. Clin. Oncol."}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914024013882?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914024013882?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:59:42Z","timestamp":1777445982000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914024013882"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":37,"alternative-id":["S0039914024013882"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2024.127009","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Construction of a chemiluminescent biosensor based on enzymatic extension and click chemistry for sensitive measurement of MGMT activity in human breast tissues","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2024.127009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"127009"}}