{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:08:02Z","timestamp":1792807682325,"version":"4.5.0"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515220189"],"award-info":[{"award-number":["2023A1515220189"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515220171"],"award-info":[{"award-number":["2023A1515220171"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515220156"],"award-info":[{"award-number":["2023A1515220156"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022A1515220033"],"award-info":[{"award-number":["2022A1515220033"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1016\/j.talanta.2025.127660","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T11:49:53Z","timestamp":1737978593000},"page":"127660","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":18,"special_numbering":"C","title":["DMSO enhanced one-pot HDA-CRISPR\/Cas12a biosensor for ultrasensitive detection of Monkeypox virus"],"prefix":"10.1016","volume":"287","author":[{"given":"Luxin","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuebiao","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8277-5480","authenticated-orcid":false,"given":"Yuanzhong","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houqi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Yi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangqing","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhui","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lifei","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1482-046X","authenticated-orcid":false,"given":"Zhangquan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.talanta.2025.127660_bib1","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00745-20","article-title":"SHERLOCK and DETECTR: CRISPR-Cas systems as potential rapid diagnostic tools for emerging infectious diseases","volume":"59","author":"Mustafa","year":"2021","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.talanta.2025.127660_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2020.112049","article-title":"Nucleic acid amplification free biosensors for pathogen detection","volume":"153","author":"Chen","year":"2020","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.talanta.2025.127660_bib3","first-page":"1","article-title":"Diagnosis, treatment, and prevention of monkeypox in children: an experts' consensus statement","author":"Jiang","year":"2022","journal-title":"World J Pediatr"},{"issue":"2","key":"10.1016\/j.talanta.2025.127660_bib4","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1134\/S0006297920020030","article-title":"Isothermal nucleic acid amplification techniques and their use in bioanalysis","volume":"85","author":"Bodulev","year":"2020","journal-title":"Biochemistry (Mosc.)"},{"issue":"8","key":"10.1016\/j.talanta.2025.127660_bib5","doi-asserted-by":"crossref","DOI":"10.3390\/cells10081931","article-title":"Loop-mediated isothermal amplification (LAMP): the better sibling of PCR?","volume":"10","author":"Soroka","year":"2021","journal-title":"Cells"},{"issue":"1","key":"10.1016\/j.talanta.2025.127660_bib6","doi-asserted-by":"crossref","DOI":"10.3390\/ani12010076","article-title":"Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research","volume":"12","author":"Padzil","year":"2021","journal-title":"Animals"},{"issue":"10","key":"10.1016\/j.talanta.2025.127660_bib7","doi-asserted-by":"crossref","DOI":"10.3390\/v14102227","article-title":"Evaluation of five buffers for inactivation of monkeypox virus and feasibility of virus detection using the panther Fusion(\u00ae) open access system","volume":"14","author":"Fischer","year":"2022","journal-title":"Viruses"},{"key":"10.1016\/j.talanta.2025.127660_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2020.12.062","article-title":"Recent advances in rolling circle amplification-based biosensing strategies-A review","volume":"1148","author":"Xu","year":"2021","journal-title":"Anal. Chim. Acta"},{"issue":"6","key":"10.1016\/j.talanta.2025.127660_bib9","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0003850","article-title":"BoHV-4-Based vector single heterologous antigen delivery protects STAT1(-\/-) mice from monkeypoxvirus lethal challenge","volume":"9","author":"Franceschi","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"issue":"24","key":"10.1016\/j.talanta.2025.127660_bib10","doi-asserted-by":"crossref","first-page":"10785","DOI":"10.1039\/D1NR00564B","article-title":"Ultrasensitive version of nucleic acid sequence-based amplification (NASBA) utilizing a nicking and extension chain reaction system","volume":"13","author":"Ju","year":"2021","journal-title":"Nanoscale"},{"issue":"3","key":"10.1016\/j.talanta.2025.127660_bib11","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1007\/s00216-017-0620-3","article-title":"Helicase-dependent isothermal amplification: a novel tool in the development of molecular-based analytical systems for rapid pathogen detection","volume":"410","author":"Barreda-Garc\u00eda","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.talanta.2025.127660_bib12","doi-asserted-by":"crossref","first-page":"15.11.1","DOI":"10.1002\/0471142727.mb1511s104","article-title":"Helicase-dependent amplification of nucleic acids","volume":"104","author":"Cao","year":"2013","journal-title":"Curr. Protoc. Mol. Biol."},{"issue":"45","key":"10.1016\/j.talanta.2025.127660_bib13","doi-asserted-by":"crossref","first-page":"15216","DOI":"10.1021\/acs.analchem.1c04133","article-title":"Strand displacement amplification assisted CRISPR-cas12a strategy for colorimetric analysis of viral nucleic acid","volume":"93","author":"Gong","year":"2021","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2025.127660_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2020.121575","article-title":"Universal linker polymerase chain reaction-triggered strand displacement amplification visual biosensor for ultra-sensitive detection of Salmonella","volume":"222","author":"Zhang","year":"2021","journal-title":"Talanta"},{"issue":"2","key":"10.1016\/j.talanta.2025.127660_bib15","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.diagmicrobio.2014.02.012","article-title":"Detection of high-risk human papillomavirus type 16 and 18 using isothermal helicase-dependent amplification","volume":"79","author":"Barbieri","year":"2014","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.talanta.2025.127660_bib16","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.biochi.2022.02.009","article-title":"PCR enhancers: types, mechanisms, and applications in long-range PCR","volume":"197","author":"Karunanathie","year":"2022","journal-title":"Biochimie"},{"issue":"2","key":"10.1016\/j.talanta.2025.127660_bib17","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1006\/abio.1993.1121","article-title":"Dimethyl sulfoxide-mediated primer Tm reduction: a method for analyzing the role of renaturation temperature in the polymerase chain reaction","volume":"209","author":"Chester","year":"1993","journal-title":"Anal. Biochem."},{"issue":"2","key":"10.1016\/j.talanta.2025.127660_bib18","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1006\/abio.2000.4931","article-title":"Dimethyl sulfoxide as additive in ready-to-use reaction mixtures for real-time polymerase chain reaction analysis with SYBR Green I dye","volume":"289","author":"Jung","year":"2001","journal-title":"Anal. Biochem."},{"issue":"4","key":"10.1016\/j.talanta.2025.127660_bib19","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1631\/jzus.B2100009","article-title":"CRISPR\/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement","volume":"22","author":"Li","year":"2021","journal-title":"J. Zhejiang Univ. - Sci. B"},{"issue":"8","key":"10.1016\/j.talanta.2025.127660_bib20","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1038\/s41580-019-0131-5","article-title":"The next generation of CRISPR-Cas technologies and applications","volume":"20","author":"Pickar-Oliver","year":"2019","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.talanta.2025.127660_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.ab.2022.114593","article-title":"Advances in amplification-free detection of nucleic acid: CRISPR\/Cas system as a powerful tool","volume":"643","author":"Qian","year":"2022","journal-title":"Anal. Biochem."},{"issue":"11","key":"10.1016\/j.talanta.2025.127660_bib22","doi-asserted-by":"crossref","first-page":"2824","DOI":"10.1021\/acssynbio.1c00401","article-title":"Advances in field detection based on CRISPR\/Cas system","volume":"10","author":"Yuan","year":"2021","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.talanta.2025.127660_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2020.113645","article-title":"Advances in biosensing: the CRISPR\/Cas system as a new powerful tool for the detection of nucleic acids","volume":"192","author":"Bonini","year":"2021","journal-title":"J. Pharm. Biomed. Anal."},{"issue":"7","key":"10.1016\/j.talanta.2025.127660_bib24","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1038\/s41551-021-00760-7","article-title":"CRISPR-based diagnostics","volume":"5","author":"Kaminski","year":"2021","journal-title":"Nat. Biomed. Eng."},{"issue":"6","key":"10.1016\/j.talanta.2025.127660_bib25","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1021\/acssynbio.9b00507","article-title":"Nucleic acid detection using CRISPR\/Cas biosensing technologies","volume":"9","author":"Aman","year":"2020","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.talanta.2025.127660_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2021.113541","article-title":"CRISPR-Cas based virus detection: recent advances and perspectives","volume":"193","author":"Yin","year":"2021","journal-title":"Biosens. Bioelectron."},{"issue":"6387","key":"10.1016\/j.talanta.2025.127660_bib27","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1126\/science.aaq0179","article-title":"Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6","volume":"360","author":"Gootenberg","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.talanta.2025.127660_bib28","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/bs.pmbts.2021.01.013","article-title":"CRISPR-based diagnostics for detection of pathogens","volume":"181","author":"Bhattacharjee","year":"2021","journal-title":"Prog Mol Biol Transl Sci"},{"key":"10.1016\/j.talanta.2025.127660_bib29","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.679994","article-title":"CRISPR-based isothermal next-generation diagnostic method for virus detection in sugarbeet","volume":"12","author":"Ramachandran","year":"2021","journal-title":"Front. Microbiol."},{"issue":"10","key":"10.1016\/j.talanta.2025.127660_bib30","doi-asserted-by":"crossref","first-page":"2986","DOI":"10.1038\/s41596-019-0210-2","article-title":"SHERLOCK: nucleic acid detection with CRISPR nucleases","volume":"14","author":"Kellner","year":"2019","journal-title":"Nat. Protoc."},{"issue":"1","key":"10.1016\/j.talanta.2025.127660_bib31","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s12967-021-02741-5","article-title":"One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR\/Cas12a","volume":"19","author":"Sun","year":"2021","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.talanta.2025.127660_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2020.112143","article-title":"An ultrasensitive and specific point-of-care CRISPR\/Cas12 based lateral flow biosensor for the rapid detection of nucleic acids","volume":"159","author":"Mukama","year":"2020","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.talanta.2025.127660_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2020.112766","article-title":"opvCRISPR: one-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection","volume":"172","author":"Wang","year":"2021","journal-title":"Biosens. Bioelectron."},{"issue":"10","key":"10.1016\/j.talanta.2025.127660_bib34","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/s41577-022-00775-4","article-title":"Monkeypox: disease epidemiology, host immunity and clinical interventions","volume":"22","author":"Lum","year":"2022","journal-title":"Nat. Rev. Immunol."},{"issue":"8","key":"10.1016\/j.talanta.2025.127660_bib35","article-title":"Monkeypox: the Re-emerging terror","volume":"14","author":"Sapkal","year":"2022","journal-title":"Cureus"},{"key":"10.1016\/j.talanta.2025.127660_bib36","doi-asserted-by":"crossref","DOI":"10.4178\/epih.e2022082","article-title":"Monkeypox: the resurgence of forgotten things","author":"Kim","year":"2022","journal-title":"Epidemiol Health"},{"issue":"7","key":"10.1016\/j.talanta.2025.127660_bib37","first-page":"11","article-title":"Monkeypox: what do we know so far? A short narrative review of literature","volume":"70","author":"Deshmukh","year":"2022","journal-title":"J. Assoc. Phys. India"},{"issue":"4","key":"10.1016\/j.talanta.2025.127660_bib38","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.virs.2022.07.006","article-title":"Monkeypox virus: a re-emergent threat to humans","volume":"37","author":"Gong","year":"2022","journal-title":"Virol. Sin."},{"issue":"1","key":"10.1016\/j.talanta.2025.127660_bib39","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jviromet.2010.07.012","article-title":"Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA","volume":"169","author":"Li","year":"2010","journal-title":"J. Virol Methods"},{"key":"10.1016\/j.talanta.2025.127660_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcv.2022.105373","article-title":"Evaluation and clinical validation of monkeypox (mpox) virus real-time PCR assays","volume":"159","author":"Mills","year":"2023","journal-title":"J. Clin. Virol."},{"issue":"15","key":"10.1016\/j.talanta.2025.127660_bib41","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.1056\/NEJMc2026172","article-title":"Detection of SARS-CoV-2 with SHERLOCK one-pot testing","volume":"383","author":"Joung","year":"2020","journal-title":"N. Engl. J. Med."}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914025001468?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914025001468?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:12:11Z","timestamp":1778631131000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914025001468"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":41,"alternative-id":["S0039914025001468"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2025.127660","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"DMSO enhanced one-pot HDA-CRISPR\/Cas12a biosensor for ultrasensitive detection of Monkeypox virus","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2025.127660","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"127660"}}