{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T17:23:30Z","timestamp":1777656210409,"version":"3.51.4"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["112-2222-E-212 -002 -MY2"],"award-info":[{"award-number":["112-2222-E-212 -002 -MY2"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["110-2113-M-110-009-MY2"],"award-info":[{"award-number":["110-2113-M-110-009-MY2"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.talanta.2025.128110","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T04:45:16Z","timestamp":1744260316000},"page":"128110","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Loading of hyaluronic acid\u2013conjugated MoS2 nanosheets with rhodamine B for sensing hyaluronidase activity in human urine and live cells"],"prefix":"10.1016","volume":"293","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3503-3216","authenticated-orcid":false,"given":"Aravindan","family":"Varatharajan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia-Chi","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9808-5863","authenticated-orcid":false,"given":"Wei\u2013Lung","family":"Tseng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.talanta.2025.128110_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/adhm.202002205","article-title":"DNA nanotechnology-based biosensors and therapeutics","volume":"10","author":"Shen","year":"2021","journal-title":"Adv. Healthcare Mater."},{"key":"10.1016\/j.talanta.2025.128110_bib2","doi-asserted-by":"crossref","first-page":"6130","DOI":"10.1021\/cr200359p","article-title":"Chemistry, biology, and medicine of fluorescent nanomaterials and related systems: new insights into biosensing, bioimaging, genomics, diagnostics, and therapy","volume":"114","author":"Yao","year":"2014","journal-title":"Chem. Rev."},{"key":"10.1016\/j.talanta.2025.128110_bib3","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.aca.2014.10.031","article-title":"Protein adsorption onto nanomaterials for the development of biosensors and analytical devices: a review","volume":"872","author":"Bhakta","year":"2015","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2025.128110_bib4","doi-asserted-by":"crossref","first-page":"17946","DOI":"10.1039\/D4RA02939A","article-title":"Advancements in enzyme immobilization on magnetic nanomaterials: toward sustainable industrial applications","volume":"14","author":"Gama Cavalcante","year":"2024","journal-title":"RSC Adv."},{"key":"10.1016\/j.talanta.2025.128110_bib5","doi-asserted-by":"crossref","first-page":"19491","DOI":"10.1039\/C6NR07249F","article-title":"When biomolecules meet graphene: from molecular level interactions to material design and applications","volume":"8","author":"Li","year":"2016","journal-title":"Nanoscale"},{"key":"10.1016\/j.talanta.2025.128110_bib6","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1007\/s10853-017-1472-0","article-title":"Surface modification of hexagonal boron nitride nanomaterials: a review","volume":"53","author":"Zheng","year":"2018","journal-title":"J. Mater. Sci."},{"key":"10.1016\/j.talanta.2025.128110_bib7","doi-asserted-by":"crossref","first-page":"9713","DOI":"10.1021\/acsnano.9b02540","article-title":"Recent advances in interface engineering of transition-metal dichalcogenides with organic molecules and polymers","volume":"13","author":"Cho","year":"2019","journal-title":"ACS Nano"},{"key":"10.1016\/j.talanta.2025.128110_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.nantod.2019.100803","article-title":"Porous MXenes: synthesis, structures, and applications","volume":"30","author":"Bu","year":"2020","journal-title":"Nano Today"},{"key":"10.1016\/j.talanta.2025.128110_bib9","doi-asserted-by":"crossref","first-page":"7004","DOI":"10.1021\/acsami.1c01089","article-title":"Metal-organic frameworks for drug delivery: a design perspective","volume":"13","author":"Lawson","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.talanta.2025.128110_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/adfm.202002046","article-title":"2D covalent organic frameworks for biomedical applications","volume":"30","author":"Bhunia","year":"2020","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.talanta.2025.128110_bib11","doi-asserted-by":"crossref","first-page":"11921","DOI":"10.1021\/acsami.5b01778","article-title":"Surface defects on natural MoS2","volume":"7","author":"Addou","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.talanta.2025.128110_bib12","doi-asserted-by":"crossref","first-page":"5998","DOI":"10.1021\/ja4019572","article-title":"Single-layer MoS2-based nanoprobes for homogeneous detection of biomolecules","volume":"135","author":"Zhu","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.talanta.2025.128110_bib13","doi-asserted-by":"crossref","first-page":"23573","DOI":"10.1039\/C7RA02649H","article-title":"MoS2-DNA and MoS2 based sensors","volume":"7","author":"Yan","year":"2017","journal-title":"RSC Adv."},{"key":"10.1016\/j.talanta.2025.128110_bib14","doi-asserted-by":"crossref","first-page":"18246","DOI":"10.1002\/chem.201803066","article-title":"Molecular functionalization of two-dimensional MoS2 nanosheets","volume":"24","author":"Stergiou","year":"2018","journal-title":"Chem. Eur J."},{"key":"10.1016\/j.talanta.2025.128110_bib15","first-page":"140","article-title":"Covalent functionalization of MoS2, mater","volume":"19","author":"Presolski","year":"2016","journal-title":"Today Off."},{"key":"10.1016\/j.talanta.2025.128110_bib16","doi-asserted-by":"crossref","first-page":"4584","DOI":"10.1021\/ja310929s","article-title":"Ligand conjugation of chemically exfoliated MoS2","volume":"135","author":"Chou","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.talanta.2025.128110_bib17","doi-asserted-by":"crossref","first-page":"12115","DOI":"10.1021\/acsnano.5b05173","article-title":"Controlled doping of vacancy-containing few-layer MoS2 via highly stable thiol-based molecular chemisorption","volume":"9","author":"Sim","year":"2015","journal-title":"ACS Nano"},{"key":"10.1016\/j.talanta.2025.128110_bib18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/adfm.201702452","article-title":"6-Mercaptopurine-Induced fluorescence quenching of monolayer MoS2 nanodots: applications to glutathione sensing, cellular imaging, and glutathione-stimulated drug delivery","volume":"27","author":"Chen","year":"2017","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.talanta.2025.128110_bib19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/aelm.202100644","article-title":"Customization of MoS2 phototransistors via thiol-based functionalization","volume":"7","author":"Im","year":"2021","journal-title":"Adv. Electron. Mater."},{"key":"10.1016\/j.talanta.2025.128110_bib20","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1039\/C9TB02770J","article-title":"Rapid, facile, reagentless, and room-temperature conjugation of monolayer MoS2 nanosheets with dual-fluorophore-labeled flares as nanoprobes for ratiometric sensing of TK1 mRNA in living cells","volume":"8","author":"Wu","year":"2020","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.talanta.2025.128110_bib21","doi-asserted-by":"crossref","first-page":"15286","DOI":"10.1021\/acsami.7b02529","article-title":"Directing assembly and disassembly of 2D MoS2 nanosheets with DNA for drug delivery","volume":"9","author":"Li","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.talanta.2025.128110_bib22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/SMMD.20230029","article-title":"Modification and crosslinking strategies for hyaluronic acid\u2010based hydrogel biomaterials","volume":"2","author":"Luo","year":"2023","journal-title":"Smart Med."},{"key":"10.1016\/j.talanta.2025.128110_bib23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/adhm.202302626","article-title":"Hyaluronic acid-based reactive oxygen species-responsive multifunctional injectable hydrogel platform accelerating diabetic wound healing","volume":"13","author":"Shi","year":"2024","journal-title":"Adv. Healthcare Mater."},{"key":"10.1016\/j.talanta.2025.128110_bib24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.carbpol.2023.121257","article-title":"The role of hyaluronic acid in the design and functionalization of nanoparticles for the treatment of colorectal cancer","volume":"320","author":"de Paula","year":"2023","journal-title":"Carbohydr. Polym."},{"key":"10.1016\/j.talanta.2025.128110_bib25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/adhm.201901182","article-title":"Binding and internalization in receptor-targeted carriers: the complex role of CD44 in the uptake of hyaluronic acid-based nanoparticles (siRNA delivery)","volume":"8","author":"Rios de la Rosa","year":"2019","journal-title":"Adv. Healthcare Mater."},{"key":"10.1016\/j.talanta.2025.128110_bib26","first-page":"1","volume":"vol. 123","author":"McAtee","year":"2014"},{"key":"10.1016\/j.talanta.2025.128110_bib27","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1136\/ard.58.3.186","article-title":"Examination of synovial fluid and serum hyaluronidase activity as a joint marker in rheumatoid arthritis and osteoarthritis patients (by zymography)","volume":"58","author":"Nagaya","year":"1999","journal-title":"Ann. Rheum. Dis."},{"key":"10.1016\/j.talanta.2025.128110_bib28","doi-asserted-by":"crossref","first-page":"42009","DOI":"10.1021\/acsami.8b15677","article-title":"Phase-dependent fluorescence quenching efficiency of MoS2 nanosheets and their applications in multiplex target biosensing","volume":"10","author":"Lan","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.talanta.2025.128110_bib29","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.apsusc.2017.12.098","article-title":"MoS 2 nanosheets as an effective fluorescent quencher for successive detection of arsenic ions in aqueous system","volume":"449","author":"Ravikumar","year":"2018","journal-title":"Appl. Surf. Sci."},{"key":"10.1016\/j.talanta.2025.128110_bib30","first-page":"33","article-title":"Two-dimensional MoS2: properties, preparation, and applications","volume":"1","author":"Li","year":"2015","journal-title":"J. Mater."},{"key":"10.1016\/j.talanta.2025.128110_bib31","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1021\/bm0493741","article-title":"Build-up of collagen and hyaluronic acid polyelectrolyte multilayers","volume":"6","author":"Johansson","year":"2005","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.talanta.2025.128110_bib32","doi-asserted-by":"crossref","first-page":"101906","DOI":"10.1063\/1.4751266","article-title":"Raman spectroscopy of the interlayer shear mode in few-layer MoS 2 flakes","volume":"101","author":"Plechinger","year":"2012","journal-title":"Appl. Phys. Lett."},{"key":"10.1016\/j.talanta.2025.128110_bib33","doi-asserted-by":"crossref","first-page":"7107","DOI":"10.1021\/acs.analchem.2c01031","article-title":"Inner filter effect correction for fluorescence measurements in microplates using variable vertical axis focus","volume":"94","author":"Weitner","year":"2022","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2025.128110_bib34","doi-asserted-by":"crossref","first-page":"11787","DOI":"10.1021\/jp1030398","article-title":"Host\u2212 guest energy transfer: semiconducting polymer nanoparticles and au nanoparticles","volume":"114","author":"Bhattacharyya","year":"2010","journal-title":"J. Phys. Chem. C"},{"key":"10.1016\/j.talanta.2025.128110_bib35","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1021\/acssensors.7b00052","article-title":"Surfen-assembled graphene oxide for fluorescence Turn-On detection of sulfated glycosaminoglycans in biological matrix","volume":"2","author":"Wang","year":"2017","journal-title":"ACS Sens."},{"key":"10.1016\/j.talanta.2025.128110_bib36","doi-asserted-by":"crossref","first-page":"17976","DOI":"10.1021\/acs.jpcc.9b04334","article-title":"Quantum ensembles of silicon nanoparticles: discrimination of static and dynamic photoluminescence quenching processes","volume":"123","author":"Hollett","year":"2019","journal-title":"J. Phys. Chem. C"},{"key":"10.1016\/j.talanta.2025.128110_bib37","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1021\/acs.langmuir.7b04240","article-title":"Fluorescence quenching investigation of methyl red adsorption on aluminum-based metal-organic frameworks","volume":"34","author":"Chen","year":"2018","journal-title":"Langmuir"},{"key":"10.1016\/j.talanta.2025.128110_bib38","doi-asserted-by":"crossref","first-page":"4071","DOI":"10.1021\/acssensors.3c01190","article-title":"Slippery viscosity-sensing platform with time readout for the detection of hyaluronidase and its inhibitor","volume":"8","author":"Bi","year":"2023","journal-title":"ACS Sens."},{"key":"10.1016\/j.talanta.2025.128110_bib39","doi-asserted-by":"crossref","first-page":"123038","DOI":"10.1016\/j.carbpol.2024.123038","article-title":"Thiol-linked hyaluronic acid-mediated encapsulation of RCR-Stabilized gold nanoclusters for hyaluronidase sensing and cellular imaging","volume":"349","author":"Madhu","year":"2025","journal-title":"Carbohydr. Polym."},{"key":"10.1016\/j.talanta.2025.128110_bib40","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.snb.2018.08.069","article-title":"Electrochemiluminescence biosensor for hyaluronidase activity detection and inhibitor assay based on the electrostatic interaction between hyaluronic acid and Ru(bpy)32+","volume":"275","author":"Li","year":"2018","journal-title":"Sensors Actuators, B Chem."},{"key":"10.1016\/j.talanta.2025.128110_bib41","doi-asserted-by":"crossref","first-page":"4601","DOI":"10.1021\/acs.molpharmaceut.2c00439","article-title":"Hyaluronic-acid-tagged cubosomes deliver cytotoxics specifically to CD44-Positive cancer cells","volume":"19","author":"Pramanik","year":"2022","journal-title":"Mol. Pharm."},{"key":"10.1016\/j.talanta.2025.128110_bib42","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1002\/cyto.a.23199","article-title":"The effect of trypan blue treatment on autofluorescence of fixed cells","volume":"91","author":"Shilova","year":"2017","journal-title":"Cytom. Part A"}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914025006009?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914025006009?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:05:27Z","timestamp":1777446327000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914025006009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":42,"alternative-id":["S0039914025006009"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2025.128110","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Loading of hyaluronic acid\u2013conjugated MoS2 nanosheets with rhodamine B for sensing hyaluronidase activity in human urine and live cells","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2025.128110","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"128110"}}