{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:46:41Z","timestamp":1792792001317,"version":"4.5.0"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100002979","name":"Chinese Academy of Sciences Dalian Institute of Chemical Physics","doi-asserted-by":"publisher","award":["2024YFA1306300"],"award-info":[{"award-number":["2024YFA1306300"]}],"id":[{"id":"10.13039\/501100002979","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002979","name":"Chinese Academy of Sciences Dalian Institute of Chemical Physics","doi-asserted-by":"publisher","award":["22274014"],"award-info":[{"award-number":["22274014"]}],"id":[{"id":"10.13039\/501100002979","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002979","name":"Chinese Academy of Sciences Dalian Institute of Chemical Physics","doi-asserted-by":"publisher","award":["2022YFC3400800"],"award-info":[{"award-number":["2022YFC3400800"]}],"id":[{"id":"10.13039\/501100002979","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002979","name":"Chinese Academy of Sciences Dalian Institute of Chemical Physics","doi-asserted-by":"publisher","award":["22034007"],"award-info":[{"award-number":["22034007"]}],"id":[{"id":"10.13039\/501100002979","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012465","name":"Dalian Medical University","doi-asserted-by":"publisher","award":["DICP UN202404"],"award-info":[{"award-number":["DICP UN202404"]}],"id":[{"id":"10.13039\/501100012465","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.talanta.2025.128595","type":"journal-article","created":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T15:29:30Z","timestamp":1752679770000},"page":"128595","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"PA","title":["Development of complementary enrichment strategies for analysis of N-linked intact glycopeptides and potential site-specific glycoforms in Alzheimer's disease"],"prefix":"10.1016","volume":"297","author":[{"given":"Zhenpeng","family":"Deng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingfa","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weibing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingming","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusong","family":"Ge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongliang","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingliang","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.talanta.2025.128595_bib1","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1038\/s41392-024-01886-1","article-title":"Glycosylation: mechanisms, biological functions and clinical implications","volume":"9","author":"He","year":"2024","journal-title":"Signal Transduct. Targeted Ther."},{"issue":"6","key":"10.1016\/j.talanta.2025.128595_bib2","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1038\/s41581-019-0129-4","article-title":"Glycosylation in health and disease","volume":"15","author":"Reily","year":"2019","journal-title":"Nat. Rev. Nephrol."},{"issue":"18","key":"10.1016\/j.talanta.2025.128595_bib3","doi-asserted-by":"crossref","first-page":"3727","DOI":"10.1007\/s00216-023-04592-z","article-title":"Recent trends in glycoproteomics by characterization of intact glycopeptides","volume":"415","author":"Piovesana","year":"2023","journal-title":"Anal. Bioanal. Chem."},{"issue":"15","key":"10.1016\/j.talanta.2025.128595_bib4","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23158251","article-title":"An integrated proteomic and glycoproteomic investigation reveals alterations in the N-Glycoproteomic network induced by 2-Deoxy-D-Glucose in colorectal cancer cells","volume":"23","author":"Ma","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"1","key":"10.1016\/j.talanta.2025.128595_bib5","doi-asserted-by":"crossref","DOI":"10.1038\/s43586-022-00128-4","article-title":"Glycoproteomics","volume":"2","author":"Bagdonaite","year":"2022","journal-title":"Nature Reviews Methods Primers"},{"issue":"1","key":"10.1016\/j.talanta.2025.128595_bib6","doi-asserted-by":"crossref","first-page":"3910","DOI":"10.1038\/s41467-022-31472-4","article-title":"Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis","volume":"13","author":"Cao","year":"2022","journal-title":"Nat. Commun."},{"issue":"2","key":"10.1016\/j.talanta.2025.128595_bib7","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.02983-21","article-title":"Aberrant cellular glycosylation may increase the ability of influenza viruses to escape host immune responses through modification of the viral glycome","volume":"13","author":"Alymova","year":"2022","journal-title":"mBio"},{"issue":"11","key":"10.1016\/j.talanta.2025.128595_bib8","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.aax8930","article-title":"Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing","volume":"5","author":"Arigoni-Affolter","year":"2019","journal-title":"Sci. Adv."},{"issue":"1","key":"10.1016\/j.talanta.2025.128595_bib9","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1038\/s41467-024-47693-8","article-title":"Exposing the molecular heterogeneity of glycosylated biotherapeutics","volume":"15","author":"Schachner","year":"2024","journal-title":"Nat. Commun."},{"issue":"11","key":"10.1016\/j.talanta.2025.128595_bib10","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1002\/cac2.12610","article-title":"Altered glycosylation in cancer: molecular functions and therapeutic potential","volume":"44","author":"Xu","year":"2024","journal-title":"Cancer Commun."},{"issue":"3","key":"10.1016\/j.talanta.2025.128595_bib11","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1016\/j.apsb.2023.10.014","article-title":"The role of N-glycosylation in cancer","volume":"14","author":"Lin","year":"2024","journal-title":"Acta Pharm. Sin. B"},{"issue":"12","key":"10.1016\/j.talanta.2025.128595_bib12","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1016\/j.tem.2021.09.006","article-title":"Emerging roles of N-linked glycosylation in brain physiology and disorders","volume":"32","author":"Conroy","year":"2021","journal-title":"Trends Endocrinol. Metabol."},{"issue":"8","key":"10.1016\/j.talanta.2025.128595_bib13","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.7150\/thno.81760","article-title":"Roles of glycosylation at the cancer cell surface: opportunities for large scale glycoproteomics","volume":"13","author":"Caval","year":"2023","journal-title":"Theranostics"},{"issue":"4","key":"10.1016\/j.talanta.2025.128595_bib14","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1515\/cclm-2018-0379","article-title":"Aberrant glycosylation and cancer biomarker discovery: a promising and thorny journey","volume":"57","author":"Wang","year":"2019","journal-title":"Clin. Chem. Lab. Med."},{"issue":"1","key":"10.1016\/j.talanta.2025.128595_bib15","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1002\/ijc.34499","article-title":"EGFR is a potential therapeutic target for highly glycosylated and aggressive pancreatic neuroendocrine neoplasms","volume":"153","author":"Xiao","year":"2023","journal-title":"Int. J. Cancer"},{"issue":"S2","key":"10.1016\/j.talanta.2025.128595_bib16","doi-asserted-by":"crossref","DOI":"10.1002\/alz.091438","article-title":"Cerebrospinal fluid apoe glycosylation associates with biomarkers of Alzheimer's disease pathology","volume":"20","author":"Nedelkov","year":"2025","journal-title":"Alzheimer's Dement."},{"key":"10.1016\/j.talanta.2025.128595_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.phrs.2024.107133","article-title":"Targeting aberrant glycosylation to modulate microglial response and improve cognition in models of Alzheimer's disease","volume":"202","author":"Wang","year":"2024","journal-title":"Pharmacol. Res."},{"issue":"4","key":"10.1016\/j.talanta.2025.128595_bib18","doi-asserted-by":"crossref","DOI":"10.1111\/bpa.13267","article-title":"Distinct patterns of plaque and microglia glycosylation in Alzheimer's disease","volume":"34","author":"Fastenau","year":"2024","journal-title":"Brain Pathol."},{"key":"10.1016\/j.talanta.2025.128595_bib19","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.jare.2023.09.002","article-title":"Plasma glycoproteomics delivers high-specificity disease biomarkers by detecting site-specific glycosylation abnormalities","volume":"61","author":"Wessels","year":"2024","journal-title":"J. Adv. Res."},{"issue":"1","key":"10.1016\/j.talanta.2025.128595_bib20","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1038\/s41420-023-01617-5","article-title":"New insight into protein glycosylation in the development of Alzheimer's disease","volume":"9","author":"Zhao","year":"2023","journal-title":"Cell Death Discov."},{"key":"10.1016\/j.talanta.2025.128595_bib21","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1016\/j.trac.2018.10.009","article-title":"Recent advances in mass spectrometry (MS)-based glycoproteomics in complex biological samples","volume":"118","author":"Chen","year":"2019","journal-title":"Trends Anal. Chem."},{"key":"10.1016\/j.talanta.2025.128595_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2023.117290","article-title":"Enrichment driven glycoproteomics: new materials, new methods, and beyond","volume":"168","author":"Xiong","year":"2023","journal-title":"TrAC, Trends Anal. Chem."},{"issue":"32","key":"10.1016\/j.talanta.2025.128595_bib23","doi-asserted-by":"crossref","first-page":"10178","DOI":"10.1002\/anie.201805165","article-title":"Recognition of complex core-fucosylated N-Glycans by a mini lectin","volume":"57","author":"Cabanettes","year":"2018","journal-title":"Angew Chem. Int. Ed. Engl."},{"issue":"29","key":"10.1016\/j.talanta.2025.128595_bib24","doi-asserted-by":"crossref","first-page":"10308","DOI":"10.1021\/acs.analchem.2c00524","article-title":"Optimized glycopeptide enrichment Method-It is all about the sauce","volume":"94","author":"Morgenstern","year":"2022","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2025.128595_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2022.340492","article-title":"Derivatization of sialylated glycopeptides plus based sialoglycopeptides enrichment using cation exchange media","volume":"1233","author":"Zhong","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2025.128595_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2019.06.020","article-title":"Recent advances in hydrophilic interaction liquid interaction chromatography materials for glycopeptide enrichment and glycan separation","volume":"124","author":"Qing","year":"2020","journal-title":"TrAC, Trends Anal. Chem."},{"key":"10.1016\/j.talanta.2025.128595_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2023.117234","article-title":"Mesoporous materials for glycopeptide separation","volume":"167","author":"Luo","year":"2023","journal-title":"TrAC, Trends Anal. Chem."},{"key":"10.1016\/j.talanta.2025.128595_bib28","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.trac.2019.02.009","article-title":"Mapping human N-linked glycoproteins and glycosylation sites using mass spectrometry","volume":"114","author":"Dang","year":"2019","journal-title":"Trends Anal. Chem."},{"issue":"5","key":"10.1016\/j.talanta.2025.128595_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2024.100762","article-title":"TIMAHAC: streamlined tandem IMAC-HILIC workflow for simultaneous and high-throughput plant phosphoproteomics and N-glycoproteomics","volume":"23","author":"Chen","year":"2024","journal-title":"Mol. Cell. Proteomics"},{"issue":"21","key":"10.1016\/j.talanta.2025.128595_bib30","doi-asserted-by":"crossref","first-page":"8822","DOI":"10.1021\/acs.analchem.4c01454","article-title":"A fully automated online enrichment and separation system for highly reproducible and In-Depth analysis of intact glycopeptide","volume":"96","author":"Hu","year":"2024","journal-title":"Anal. Chem."},{"issue":"14","key":"10.1016\/j.talanta.2025.128595_bib31","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adk6911","article-title":"Human brain glycoform coregulation network and glycan modification alterations in Alzheimer's disease","volume":"10","author":"Zhang","year":"2024","journal-title":"Sci. Adv."},{"key":"10.1016\/j.talanta.2025.128595_bib32","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.talanta.2019.02.010","article-title":"A multi-parallel N-glycopeptide enrichment strategy for high-throughput and in-depth mapping of the N-glycoproteome in metastatic human hepatocellular carcinoma cell lines","volume":"199","author":"Jiang","year":"2019","journal-title":"Talanta"},{"key":"10.1016\/j.talanta.2025.128595_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2021.100081","article-title":"In-depth site-specific analysis of N-glycoproteome in human cerebrospinal fluid and glycosylation landscape changes in Alzheimer's disease","volume":"20","author":"Chen","year":"2021","journal-title":"Mol. Cell. Proteomics"},{"issue":"8","key":"10.1016\/j.talanta.2025.128595_bib34","doi-asserted-by":"crossref","first-page":"5143","DOI":"10.1002\/alz.13859","article-title":"Revised criteria for diagnosis and staging of Alzheimer's disease: alzheimer's association workgroup","volume":"20","author":"Jack","year":"2024","journal-title":"Alzheimer's Dement."},{"issue":"20","key":"10.1016\/j.talanta.2025.128595_bib35","doi-asserted-by":"crossref","first-page":"7473","DOI":"10.1021\/acs.analchem.1c00645","article-title":"Automated intact glycopeptide enrichment method facilitating highly reproducible analysis of serum site-specific N-Glycoproteome","volume":"93","author":"Liu","year":"2021","journal-title":"Anal. Chem."},{"issue":"1","key":"10.1016\/j.talanta.2025.128595_bib36","doi-asserted-by":"crossref","first-page":"1900","DOI":"10.1038\/s41467-022-29530-y","article-title":"Glyco-decipher enables glycan database-independent peptide matching and in-depth characterization of site-specific N-glycosylation","volume":"13","author":"Fang","year":"2022","journal-title":"Nat. Commun."},{"issue":"40","key":"10.1016\/j.talanta.2025.128595_bib37","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abc5802","article-title":"Integrative glycoproteomics reveals protein N-glycosylation aberrations and glycoproteomic network alterations in Alzheimer's disease","volume":"6","author":"Zhang","year":"2020","journal-title":"Sci. Adv."},{"issue":"16","key":"10.1016\/j.talanta.2025.128595_bib38","doi-asserted-by":"crossref","DOI":"10.1002\/cbic.202300017","article-title":"Dessert or poison? The roles of glycosylation in alzheimer's, parkinson's, Huntington's disease, and amyotrophic lateral sclerosis","volume":"24","author":"Yang","year":"2023","journal-title":"Chembiochem"},{"issue":"20","key":"10.1016\/j.talanta.2025.128595_bib39","doi-asserted-by":"crossref","first-page":"5041","DOI":"10.1039\/D3AN01018J","article-title":"Characterization of site-specific N-glycosylation signatures of isolated uromodulin from human urine","volume":"148","author":"Lin","year":"2023","journal-title":"Analyst"},{"issue":"10","key":"10.1016\/j.talanta.2025.128595_bib40","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1021\/acs.jproteome.9b00504","article-title":"N-Glycan profile of cerebrospinal fluids from alzheimer's disease patients using liquid chromatography with mass spectrometry","volume":"18","author":"Cho","year":"2019","journal-title":"J. Proteome Res."},{"issue":"12","key":"10.1016\/j.talanta.2025.128595_bib41","doi-asserted-by":"crossref","first-page":"5063","DOI":"10.1021\/acs.jproteome.5b00892","article-title":"Glycosylation of human plasma clusterin yields a novel candidate biomarker of alzheimer's disease","volume":"14","author":"Liang","year":"2015","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.talanta.2025.128595_bib42","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jprot.2015.10.006","article-title":"CSF N-glycoproteomics for early diagnosis in Alzheimer's disease","volume":"131","author":"Palmigiano","year":"2016","journal-title":"J. Proteonomics"},{"issue":"15","key":"10.1016\/j.talanta.2025.128595_bib43","doi-asserted-by":"crossref","first-page":"3221","DOI":"10.1111\/febs.15197","article-title":"Glycan biomarkers for Alzheimer disease correlate with T\u2010tau and P\u2010tau in cerebrospinal fluid in subjective cognitive impairment","volume":"287","author":"Schedin\u2010Weiss","year":"2020","journal-title":"FEBS J."},{"issue":"D1","key":"10.1016\/j.talanta.2025.128595_bib44","doi-asserted-by":"crossref","first-page":"D462","DOI":"10.1093\/nar\/gkae1032","article-title":"jPOST environment accelerates the reuse and reanalysis of public proteome mass spectrometry data","volume":"53","author":"Okuda","year":"2025","journal-title":"Nucleic Acids Res."}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914025010859?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914025010859?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:13:40Z","timestamp":1777461220000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914025010859"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":44,"alternative-id":["S0039914025010859"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2025.128595","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of complementary enrichment strategies for analysis of N-linked intact glycopeptides and potential site-specific glycoforms in Alzheimer's disease","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2025.128595","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"128595"}}