{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T02:14:12Z","timestamp":1793240052793,"version":"4.5.0"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100015406","name":"Tianjin Municipal Science and Technology Bureau","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100015406","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019065","name":"Tianjin Municipal Science and Technology Program","doi-asserted-by":"publisher","award":["24ZXZSSS00350"],"award-info":[{"award-number":["24ZXZSSS00350"]}],"id":[{"id":"10.13039\/501100019065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.talanta.2025.129116","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T11:41:48Z","timestamp":1763034108000},"page":"129116","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["A comprehensive characterization strategy for natural products using dual-mode hybrid acquisition on high-resolution MS with intelligent subdivision polygonal mass defect filtering: a case study on Typhae Pollen"],"prefix":"10.1016","volume":"299","author":[{"given":"Chengda","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xudong","family":"Zhen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruolin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunze","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxu","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.talanta.2025.129116_bib1","article-title":"The application of UHPLC-HRMS for quality control of traditional Chinese medicine","volume":"13","author":"Ma","year":"2022","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.talanta.2025.129116_bib2","doi-asserted-by":"crossref","DOI":"10.3390\/molecules27103071","article-title":"A comparative study on analysis of ginsenosides in American ginseng root residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS\/MS","volume":"27","author":"Hsu","year":"2022","journal-title":"Molecules"},{"key":"10.1016\/j.talanta.2025.129116_bib3","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.chroma.2018.03.044","volume":"1550","author":"Ren","year":"2018","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.talanta.2025.129116_bib4","article-title":"Profiling of phenolics in cashew nut (Anacardium occidentale L.) testa and evaluation of their antioxidant and antimicrobial properties","volume":"51","author":"Sruthi","year":"2022","journal-title":"Food Biosci."},{"key":"10.1016\/j.talanta.2025.129116_bib5","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1002\/jms.2953","article-title":"The use of mass defect in modern mass spectrometry","volume":"47","author":"Sleno","year":"2012","journal-title":"J. Mass Spectrom."},{"key":"10.1016\/j.talanta.2025.129116_bib6","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1002\/jms.1610","article-title":"Mass defect filter technique and its applications to drug metabolite identification by high-resolution mass spectrometry","volume":"44","author":"Zhang","year":"2009","journal-title":"J. Mass Spectrom."},{"key":"10.1016\/j.talanta.2025.129116_bib7","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1080\/00498254.2020.1744047","article-title":"Metabolic profiling of coumarins by the combination of UPLC-MS-based metabolomics and multiple mass defect filter","volume":"50","author":"Xiao","year":"2020","journal-title":"Xenobiotica"},{"key":"10.1016\/j.talanta.2025.129116_bib8","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.jpba.2010.05.004","article-title":"Rapid and global detection and characterization of aconitum alkaloids in Yin Chen Si Ni Tang, a traditional Chinese medical formula, by ultra performance liquid chromatography-high resolution mass spectrometry and automated data analysis","volume":"53","author":"Yan","year":"2010","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.talanta.2025.129116_bib9","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1002\/pca.2508","article-title":"Rapid identification of polymethoxylated flavonoids in traditional Chinese medicines with a practical strategy of stepwise mass defect filtering coupled to diagnostic product ions analysis based on a hybrid LTQ-Orbitrap mass spectrometer","volume":"25","author":"Zhang","year":"2014","journal-title":"Phytochem. Anal."},{"key":"10.1016\/j.talanta.2025.129116_bib10","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.chroma.2018.05.066","article-title":"An enhanced strategy integrating offline two-dimensional separation and step-wise precursor ion list-based raster-mass defect filter: characterization of indole alkaloids in five botanical origins of Uncariae Ramulus Cum Unicis as an exemplary application","volume":"1563","author":"Pan","year":"2018","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.talanta.2025.129116_bib11","doi-asserted-by":"crossref","first-page":"7817","DOI":"10.1007\/s00216-019-02180-8","article-title":"In-depth profiling, characterization, and comparison of the ginsenosides among three different parts (the root, stem leaf, and flower bud) of Panax quinquefolius L. by ultra-high performance liquid chromatography\/quadrupole-Orbitrap mass spectrometry","volume":"411","author":"Wang","year":"2019","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.talanta.2025.129116_bib12","doi-asserted-by":"crossref","first-page":"2821","DOI":"10.1007\/s00216-020-03020-w","article-title":"Dual-polarity SALDI FT-ICR MS imaging and Kendrick mass defect data filtering for lipid analysis","volume":"413","author":"M\u00fcller","year":"2020","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.talanta.2025.129116_bib13","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.jpba.2015.02.028","article-title":"An integrated high resolution mass spectrometric data acquisition method for rapid screening of saponins in Panax notoginseng (Sanqi)","volume":"109","author":"Lai","year":"2015","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.talanta.2025.129116_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2021.462178","article-title":"A strategy for intelligent chemical profiling-guided precise quantitation of multi-components in traditional Chinese medicine formulae-QiangHuoShengShi decoction","volume":"1649","author":"Guo","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.talanta.2025.129116_bib15","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1002\/rcm.3951","article-title":"Generic dealkylation: a tool for increasing the hit-rate of metabolite rationalization, and automatic customization of mass defect filters","volume":"23","author":"Mortishire-Smith","year":"2009","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"10.1016\/j.talanta.2025.129116_bib16","doi-asserted-by":"crossref","first-page":"8072","DOI":"10.1021\/acs.analchem.9b05135","article-title":"Comparison of full-scan, data-dependent, and data-independent acquisition modes in liquid chromatography-mass spectrometry based untargeted metabolomics","volume":"92","author":"Guo","year":"2020","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2025.129116_bib17","doi-asserted-by":"crossref","first-page":"12964","DOI":"10.1021\/acs.analchem.3c02888","article-title":"Improving the data quality of untargeted metabolomics through a targeted data-dependent acquisition based on an inclusion list of differential and preidentified ions","volume":"95","author":"Zhang","year":"2023","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2025.129116_bib18","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.chroma.2018.11.024","article-title":"Simultaneously targeted and untargeted multicomponent characterization of Erzhi Pill by offline two-dimensional liquid chromatography\/quadrupole-Orbitrap mass spectrometry","volume":"1584","author":"Fu","year":"2018","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.talanta.2025.129116_bib19","doi-asserted-by":"crossref","DOI":"10.3390\/ijms231911601","article-title":"Proteomics profiling of stool samples from preterm neonates with SWATH\/DIA mass spectrometry for predicting necrotizing enterocolitis","volume":"23","author":"Gagn\u00e9","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.talanta.2025.129116_bib20","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.201700304","article-title":"Comparative analyses of data independent acquisition mass spectrometric approaches: DIA, WiSIM\u2010DIA, and untargeted DIA","volume":"18","author":"Koopmans","year":"2018","journal-title":"Proteomics"},{"key":"10.1016\/j.talanta.2025.129116_bib21","doi-asserted-by":"crossref","first-page":"2901","DOI":"10.3390\/molecules27092901","article-title":"Flavonoids as potential anti-inflammatory molecules: a review","volume":"27","author":"Al-Khayri","year":"2022","journal-title":"Molecules"},{"key":"10.1016\/j.talanta.2025.129116_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2021.111596","article-title":"Antiviral activities of flavonoids","volume":"140","author":"Badshah","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.talanta.2025.129116_bib23","doi-asserted-by":"crossref","first-page":"457","DOI":"10.3390\/nu12020457","article-title":"Flavonoids as anticancer agents","volume":"12","author":"Kopustinskiene","year":"2020","journal-title":"Nutrients"},{"key":"10.1016\/j.talanta.2025.129116_bib24","doi-asserted-by":"crossref","first-page":"691","DOI":"10.3389\/fphar.2018.00691","article-title":"Screening of combinatorial quality markers for natural products by metabolomics coupled with chemometrics. A case study on Pollen Typhae","volume":"9","author":"Ding","year":"2018","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.talanta.2025.129116_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2020.113774","article-title":"Effects of carbonized process on quality control, chemical composition and pharmacology of Typhae Pollen: a review","volume":"270","author":"Gao","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.talanta.2025.129116_bib26","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.jep.2025.120124","article-title":"Exploring the mechanism of Carbonized Typhae Pollen in treating blood stasis syndrome through metabolic profiling: the synergistic effect of hemostasis without blood stasis","volume":"351","author":"Zhang","year":"2025","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.talanta.2025.129116_bib27","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1080\/13880209.2019.1645700","article-title":"A review on charred traditional Chinese herbs: carbonization to yield a haemostatic effect","volume":"57","author":"Chen","year":"2019","journal-title":"Pharm. Biol."},{"key":"10.1016\/j.talanta.2025.129116_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.phymed.2022.154152","article-title":"Discovery of processing-associated Q-marker of carbonized traditional Chinese medicine: an integrated strategy of metabolomics, systems pharmacology and in vivo high-throughput screening model","volume":"102","author":"Gao","year":"2022","journal-title":"Phytomedicine"},{"key":"10.1016\/j.talanta.2025.129116_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2023.115619","article-title":"Identifying the active ingredients of carbonized Typhae Pollen by spectrum-effect relationship combined with MBPLS, PLS, and SVM algorithms","volume":"235","author":"Ouyang","year":"2023","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.talanta.2025.129116_bib30","first-page":"836","article-title":"Ethanolic extract of Puhuang (Pollen Typhae) modulates lipopolysaccharide-induced inflammatory response through inducible nitric oxide synthase\/cyclooxygenase-2 signaling in RAW 264.7 macrophage","volume":"41","author":"Chen","year":"2021","journal-title":"J. Tradit. Chin. Med."},{"key":"10.1016\/j.talanta.2025.129116_bib31","first-page":"1669","article-title":"Pollen typhae total flavone suppresses A7r5 cell proliferation promoted by insulin involving the MEK1\/2-ERK1\/2 cascades","volume":"35","author":"Feng","year":"2022","journal-title":"Pak. J. Pharm. Sci."},{"key":"10.1016\/j.talanta.2025.129116_bib32","article-title":"Evaluation of VEGF mediated pro-angiogenic and hemostatic effects and chemical marker investigation for Typhae Pollen and its processed product","volume":"268","author":"Gao","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.talanta.2025.129116_bib33","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1007\/s11655-019-3052-4","article-title":"Pollen Typhae total flavone inhibits endoplasmic reticulum stress-induced apoptosis in human aortic-vascular smooth muscle cells through down-regulating PERK-eIF2\u03b1-ATF4-CHOP pathway","volume":"25","author":"Chen","year":"2019","journal-title":"Chin. J. Integr. Med."},{"key":"10.1016\/j.talanta.2025.129116_bib34","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1186\/s12951-025-03415-w","article-title":"Advances in brain-targeted delivery strategies and natural product-mediated enhancement of blood\u2013brain barrier permeability","volume":"23","author":"Liu","year":"2025","journal-title":"J. Nanobiotechnol."},{"key":"10.1016\/j.talanta.2025.129116_bib35","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1186\/s12951-024-02981-9","article-title":"An encounter between metal ions and natural products: natural products-coordinated metal ions for the diagnosis and treatment of tumors","volume":"22","author":"Liu","year":"2024","journal-title":"J. Nanobiotechnol."},{"key":"10.1016\/j.talanta.2025.129116_bib36","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1002\/pca.3519","article-title":"Comprehensive quality evaluation and botanical differentiation of Typhae Pollen using UHPLC-DAD\/Q-TOF-MS and multivariate chemometric analysis","volume":"36","author":"Tian","year":"2025","journal-title":"Phytochem. Anal."},{"key":"10.1016\/j.talanta.2025.129116_bib37","doi-asserted-by":"crossref","first-page":"18352","DOI":"10.3390\/molecules201018352","article-title":"Characterization and quantification by LC-MS\/MS of the chemical components of the heating products of the flavonoids extract in Pollen Typhae for transformation rule exploration","volume":"20","author":"Chen","year":"2015","journal-title":"Molecules"}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914025016078?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914025016078?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T12:48:25Z","timestamp":1773838105000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914025016078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":37,"alternative-id":["S0039914025016078"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2025.129116","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A comprehensive characterization strategy for natural products using dual-mode hybrid acquisition on high-resolution MS with intelligent subdivision polygonal mass defect filtering: a case study on Typhae Pollen","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2025.129116","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"129116"}}