{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:25:51Z","timestamp":1790508351315,"version":"4.1.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2022J01096"],"award-info":[{"award-number":["2022J01096"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008859","name":"Fuzhou University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008859","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22276033"],"award-info":[{"award-number":["22276033"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2026,5]]},"DOI":"10.1016\/j.talanta.2026.129458","type":"journal-article","created":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T23:17:01Z","timestamp":1769296621000},"page":"129458","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Aluminum-copper layered double hydroxide-enabled electrochemiluminescence-multicolor dual-mode biosensor for heparin-binding protein detection in sepsis diagnosis"],"prefix":"10.1016","volume":"302","author":[{"given":"Zhenjie","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengqi","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuiying","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7890-6812","authenticated-orcid":false,"given":"Zhenyu","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"8","key":"10.1016\/j.talanta.2026.129458_bib1","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1001\/jama.2016.0287","article-title":"The third international consensus definitions for sepsis and septic shock (sepsis-3)","volume":"315","author":"Singer","year":"2016","journal-title":"JAMA"},{"issue":"1","key":"10.1016\/j.talanta.2026.129458_bib2","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1186\/s13054-019-2663-7","article-title":"The SOFA score-development, utility and challenges of accurate assessment in clinical trials","volume":"23","author":"Lambden","year":"2019","journal-title":"Crit. Care"},{"key":"10.1016\/j.talanta.2026.129458_bib3","doi-asserted-by":"crossref","first-page":"tkz006","DOI":"10.1093\/burnst\/tkz006","article-title":"Prediction of median survival time in sepsis patients by the SOFA score combined with different predictors","volume":"8","author":"Li","year":"2020","journal-title":"Burns Trauma"},{"issue":"4","key":"10.1016\/j.talanta.2026.129458_bib4","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1097\/MEJ.0000000000001235","article-title":"Diagnostic performances of procalcitonin and C-reactive protein for sepsis: a systematic review and meta-analysis","volume":"32","author":"Chuang","year":"2025","journal-title":"Eur. J. Emerg. Med."},{"issue":"4","key":"10.1016\/j.talanta.2026.129458_bib5","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1097\/SLA.0000000000005198","article-title":"Biomarkers for the early diagnosis of sepsis in burns: systematic review and meta-analysis","volume":"275","author":"Li","year":"2022","journal-title":"Ann. Surg."},{"issue":"1","key":"10.1016\/j.talanta.2026.129458_bib6","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s40635-016-0104-3","article-title":"Heparin-binding protein is important for vascular leak in sepsis","volume":"4","author":"Bentzer","year":"2016","journal-title":"Intensive Care Med. Exp."},{"issue":"11","key":"10.1016\/j.talanta.2026.129458_bib7","doi-asserted-by":"crossref","first-page":"2378","DOI":"10.1097\/CCM.0000000000001265","article-title":"Heparin-binding protein measurement improves the prediction of severe infection with organ dysfunction in the emergency department","volume":"43","author":"Linder","year":"2015","journal-title":"Crit. Care Med."},{"issue":"6","key":"10.1016\/j.talanta.2026.129458_bib8","first-page":"654","article-title":"Predictive value of heparin binding protein for sepsis","volume":"33","author":"Zhang","year":"2021","journal-title":"Zhonghua Wei Zhong Bing Ji Jiu Yi Xue"},{"issue":"4","key":"10.1016\/j.talanta.2026.129458_bib9","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1097\/CCM.0000000000002262","article-title":"Increased time to initial antimicrobial administration is associated with progression to septic shock in severe sepsis patients","volume":"45","author":"Whiles","year":"2017","journal-title":"Crit. Care Med."},{"key":"10.1016\/j.talanta.2026.129458_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.intimp.2024.111616","article-title":"Effect of delayed antibiotic use on mortality outcomes in patients with sepsis or septic shock: a systematic review and meta-analysis","volume":"129","author":"Tang","year":"2024","journal-title":"Int. Immunopharmacol."},{"issue":"1","key":"10.1016\/j.talanta.2026.129458_bib11","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1186\/s12931-021-01764-2","article-title":"Heparin-binding protein in lower airway samples as a biomarker for pneumonia","volume":"22","author":"Paulsson","year":"2021","journal-title":"Respir. Res."},{"key":"10.1016\/j.talanta.2026.129458_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.ab.2021.114359","article-title":"Establishment of heparin-binding protein time-resolved immunoassay and some potential clinical applications","volume":"631","author":"Xiang","year":"2021","journal-title":"Anal. Biochem."},{"issue":"8","key":"10.1016\/j.talanta.2026.129458_bib13","doi-asserted-by":"crossref","first-page":"1930","DOI":"10.3390\/diagnostics12081930","article-title":"Reference interval for the axis-shield clinical chemistry heparin-binding protein assay","volume":"12","author":"Yoon","year":"2022","journal-title":"Diagnostics (Basel)"},{"issue":"13","key":"10.1016\/j.talanta.2026.129458_bib14","doi-asserted-by":"crossref","first-page":"7880","DOI":"10.1021\/acs.analchem.7b05470","article-title":"Environmental stability of plasmonic biosensors based on natural versus artificial antibody","volume":"90","author":"Luan","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2026.129458_bib15","doi-asserted-by":"crossref","DOI":"10.1038\/srep16206","article-title":"Peptide functionalized gold nanorods for the sensitive detection of a cardiac biomarker using plasmonic paper devices","volume":"5","author":"Tadepalli","year":"2015","journal-title":"Sci. Rep."},{"issue":"23","key":"10.1016\/j.talanta.2026.129458_bib16","doi-asserted-by":"crossref","first-page":"7432","DOI":"10.3390\/s24237432","article-title":"Recent developments in aptamer-based sensors for diagnostics","volume":"24","author":"Sheraz","year":"2024","journal-title":"Sensors (Basel)"},{"key":"10.1016\/j.talanta.2026.129458_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2023.117408","article-title":"Aptamer-based point-of-care-testing for small molecule targets: from aptamers to aptasensors, devices and applications","volume":"169","author":"Zhao","year":"2023","journal-title":"TrAC, Trends Anal. Chem."},{"issue":"8","key":"10.1016\/j.talanta.2026.129458_bib18","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.3390\/molecules29081717","article-title":"Screening and application of DNA aptamers for heparin-binding protein","volume":"29","author":"Zhou","year":"2024","journal-title":"Molecules"},{"issue":"23","key":"10.1016\/j.talanta.2026.129458_bib19","doi-asserted-by":"crossref","first-page":"5247","DOI":"10.3390\/s19235247","article-title":"Hydrogel fluorescence microsensor with fluorescence recovery for prolonged stable temperature measurements","volume":"19","author":"Hashim","year":"2019","journal-title":"Sensors (Basel)"},{"key":"10.1016\/j.talanta.2026.129458_bib20","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1016\/j.talanta.2013.07.072","article-title":"Role of background observed in aptasensor with chemiluminescence detection","volume":"116","author":"Park","year":"2013","journal-title":"Talanta"},{"issue":"9","key":"10.1016\/j.talanta.2026.129458_bib21","doi-asserted-by":"crossref","first-page":"738","DOI":"10.3390\/bios12090738","article-title":"Electrochemiluminescence systems for the detection of biomarkers: strategical and technological advances","volume":"12","author":"Yoo","year":"2022","journal-title":"Biosensors (Basel)"},{"issue":"5","key":"10.1016\/j.talanta.2026.129458_bib22","doi-asserted-by":"crossref","first-page":"2853","DOI":"10.1021\/acs.analchem.4c05379","article-title":"Kirkendall effect-mediated transformation of ZIF-67 to NiCo-LDH nanocages as oxidase mimics for multicolor point-of-care testing of \u03b2-galactosidase activity and Escherichia coli","volume":"97","author":"Chen","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2026.129458_bib23","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.bios.2016.08.021","article-title":"A universal multicolor immunosensor for semiquantitative visual detection of biomarkers with the naked eyes","volume":"87","author":"Ma","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.talanta.2026.129458_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2025.137406","article-title":"Multicolor visual biosensor based on dual-functional aptamer-mediated gold nanorod etching for point-of-care testing of \u03b2-lactoglobulin","volume":"431","author":"Sun","year":"2025","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.talanta.2026.129458_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2020.128604","article-title":"Manganese dioxide nanosheet-mediated etching of gold nanorods for a multicolor colorimetric assay of total antioxidant capacity","volume":"321","author":"Tian","year":"2020","journal-title":"Sensor. Actuator. B Chem."},{"key":"10.1016\/j.talanta.2026.129458_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2024.117755","article-title":"Dual-mode aptasensors with cross validation capacity for reliability enhancement and analytical assurance","volume":"177","author":"Zhao","year":"2024","journal-title":"TrAC, Trends Anal. Chem."},{"key":"10.1016\/j.talanta.2026.129458_bib27","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.ijbiomac.2020.10.063","article-title":"Efficient immobilization of aptamers on the layered double hydroxide nanohybrids for the electrochemical proteins detection","volume":"166","author":"Konari","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.talanta.2026.129458_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.apcata.2024.119563","article-title":"Catalytic removal of malachite green from aqueous solution by a peroxidase-mimicking Cu-Al layered double hydroxide nanoparticle: synthesis, characterization, and application","volume":"670","author":"Khodakarami","year":"2024","journal-title":"Appl. Catal. Gen."},{"key":"10.1016\/j.talanta.2026.129458_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2024.150612","article-title":"Research progress of nanozymes in colorimetric biosensing: classification, activity and application","volume":"487","author":"Yang","year":"2024","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.talanta.2026.129458_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.ccr.2021.214170","article-title":"Nanozymes: activity origin, catalytic mechanism, and biological application","volume":"448","author":"Yang","year":"2021","journal-title":"Coord. Chem. Rev."},{"issue":"11","key":"10.1016\/j.talanta.2026.129458_bib31","doi-asserted-by":"crossref","first-page":"2809","DOI":"10.3390\/nano11112809","article-title":"Recent advances in layered double hydroxide-based electrochemical and optical sensors","volume":"11","author":"Kim","year":"2021","journal-title":"Nanomaterials (Basel)"},{"issue":"1","key":"10.1016\/j.talanta.2026.129458_bib32","doi-asserted-by":"crossref","first-page":"9461","DOI":"10.1038\/s41598-022-13431-7","article-title":"Multifunctional green synthesized Cu-Al layered double hydroxide (LDH) nanoparticles: anti-cancer and antibacterial activities","volume":"12","author":"Kiani","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.talanta.2026.129458_bib33","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1016\/j.cej.2015.05.090","article-title":"Catalytic oxidation of styrene over Cu-doped hydrotalcites","volume":"279","author":"Tien Thao","year":"2015","journal-title":"Chem. Eng. J."},{"issue":"46","key":"10.1016\/j.talanta.2026.129458_bib34","doi-asserted-by":"crossref","first-page":"29051","DOI":"10.1039\/C7RA03848H","article-title":"A novel three-dimensional hierarchical CuAl layered double hydroxide with excellent catalytic activity for degradation of methyl orange","volume":"7","author":"Li","year":"2017","journal-title":"RSC Adv."},{"issue":"21","key":"10.1016\/j.talanta.2026.129458_bib35","doi-asserted-by":"crossref","first-page":"4826","DOI":"10.1039\/b103656b","article-title":"Synthesis, characterization and catalytic hydroxylation of phenol over CuCoAl ternary hydrotalcites","volume":"3","author":"Rives","year":"2001","journal-title":"Phys. Chem. Chem. Phys."},{"issue":"18","key":"10.1016\/j.talanta.2026.129458_bib36","doi-asserted-by":"crossref","first-page":"15510","DOI":"10.1021\/acsami.7b02987","article-title":"2-methylimidazole-derived Ni-Co layered double hydroxide nanosheets as high rate capability and high energy density storage material in hybrid supercapacitors","volume":"9","author":"Wang","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"6","key":"10.1016\/j.talanta.2026.129458_bib37","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1007\/s13201-022-01668-6","article-title":"Synthesis of CuAl-layered double hydroxide\/MgO2 nanocomposite catalyst for the degradation of organic dye under dark condition","volume":"12","author":"Aragaw","year":"2022","journal-title":"Appl. Water Sci."},{"key":"10.1016\/j.talanta.2026.129458_bib38","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.jenvman.2018.05.037","article-title":"Multipath fabrication of hierarchical CuAl layered double hydroxide\/carbon fiber composites for the degradation of ammonia nitrogen","volume":"220","author":"Peng","year":"2018","journal-title":"J. Environ. Manag."},{"issue":"9","key":"10.1016\/j.talanta.2026.129458_bib39","doi-asserted-by":"crossref","first-page":"3372","DOI":"10.3390\/ma16093372","article-title":"Engineering Cu\/NiCu LDH heterostructure nanosheet arrays for highly-efficient water oxidation","volume":"16","author":"Wang","year":"2023","journal-title":"Materials (Basel)"},{"issue":"16","key":"10.1016\/j.talanta.2026.129458_bib40","doi-asserted-by":"crossref","first-page":"2713","DOI":"10.1080\/00032719.2024.2434111","article-title":"Colorimetric determination of glutathione (GSH) using metal-organic framework (MOF) NiCo layered double hydroxide (LDH) as a peroxidase nanozyme mimic","volume":"58","author":"Wang","year":"2024","journal-title":"Anal. Lett."},{"key":"10.1016\/j.talanta.2026.129458_bib41","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.ijleo.2017.12.138","article-title":"Investigation of optical bandgap variation and photoluminescence behavior in nanocrystalline CuO thin films","volume":"158","author":"Xu","year":"2018","journal-title":"Optik"},{"key":"10.1016\/j.talanta.2026.129458_bib42","doi-asserted-by":"crossref","DOI":"10.3389\/fchem.2022.931767","article-title":"Electrochemical reduction of CO2 with good efficiency on a nanostructured Cu-Al catalyst","volume":"10","author":"Zeng","year":"2022","journal-title":"Front. Chem."},{"issue":"1","key":"10.1016\/j.talanta.2026.129458_bib43","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.ijhydene.2022.09.197","article-title":"CuAl layered double hydroxide as a highly efficient electrocatalyst for the electrolysis of water into hydrogen and oxygen fuels","volume":"48","author":"Lokesh","year":"2023","journal-title":"Int. J. Hydrogen Energy"},{"issue":"47","key":"10.1016\/j.talanta.2026.129458_bib44","doi-asserted-by":"crossref","first-page":"35184","DOI":"10.1039\/D4RA06413E","article-title":"Probing intrinsic defects of aluminium-doped CuO thin films for solar cell applications","volume":"14","author":"Prakash","year":"2024","journal-title":"RSC Adv."},{"issue":"1","key":"10.1016\/j.talanta.2026.129458_bib45","doi-asserted-by":"crossref","first-page":"5702","DOI":"10.1038\/s41598-025-89241-4","article-title":"Accuracy of blood heparin-binding protein (HBP) for diagnosis bacteremia in patients with sepsis","volume":"15","author":"Mao","year":"2025","journal-title":"Sci. Rep."}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S003991402600113X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S003991402600113X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T08:23:35Z","timestamp":1781943815000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S003991402600113X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":45,"alternative-id":["S003991402600113X"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2026.129458","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Aluminum-copper layered double hydroxide-enabled electrochemiluminescence-multicolor dual-mode biosensor for heparin-binding protein detection in sepsis diagnosis","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2026.129458","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"129458"}}