{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:34:48Z","timestamp":1786696488943,"version":"build-2736575974"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["2026-RISE-11-013-01"],"award-info":[{"award-number":["2026-RISE-11-013-01"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["2026-RISE-11-013-03"],"award-info":[{"award-number":["2026-RISE-11-013-03"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003661","name":"Korea Institute for Advancement of Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002454","name":"Cheongju University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002454","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003081","name":"Ministry of Education, Ethiopia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003081","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003052","name":"Ministry of Trade, Industry and Energy","doi-asserted-by":"publisher","award":["P0017805"],"award-info":[{"award-number":["P0017805"]}],"id":[{"id":"10.13039\/501100003052","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Talanta"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.talanta.2026.130094","type":"journal-article","created":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T09:52:20Z","timestamp":1780825940000},"page":"130094","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Quantitative analysis of bovine angiogenin in milk using a microliter-volume protein microarray"],"prefix":"10.1016","volume":"310","author":[{"given":"Kyong-Mi","family":"Min","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7495-0621","authenticated-orcid":false,"given":"Sang-Oh","family":"Ha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0077-283X","authenticated-orcid":false,"given":"Si-Eun","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1719-3132","authenticated-orcid":false,"given":"Hyun-Bin","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9533-9581","authenticated-orcid":false,"given":"Jae-Won","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.talanta.2026.130094_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2024.117110","article-title":"Development of multiple genome-wide proteome microarrays comprised wafer substrate-based chip and its scanner: an advanced high-throughput and sensitivity for molecular interactions studies","volume":"272","author":"Yang","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.talanta.2026.130094_bib2","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2025.1650094","article-title":"High-throughput screening of monoclonal antibodies against carbapenemases using a multiplex protein microarray platform","volume":"16","author":"Braun","year":"2025","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.talanta.2026.130094_bib3","doi-asserted-by":"crossref","DOI":"10.1155\/2024\/4454744","article-title":"Proteomics and its current application in biomedical area: concise review","volume":"2024","author":"Gobena","year":"2024","journal-title":"ScientificWorldJournal"},{"key":"10.1016\/j.talanta.2026.130094_bib4","doi-asserted-by":"crossref","DOI":"10.1155\/2023\/5510791","article-title":"Diagnostic and therapeutic application of proteomics in infectious disease","volume":"2023","author":"Yihunie","year":"2023","journal-title":"Adv. Cell Gene Ther."},{"key":"10.1016\/j.talanta.2026.130094_bib5","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.cbpa.2015.10.013","article-title":"Applications in high-content functional protein microarrays","volume":"30","author":"Moore","year":"2016","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.talanta.2026.130094_bib6","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1002\/pmic.200900146","article-title":"Site-specific inhibition of integrin alpha v beta 3-vitronectin association by a Ser-Asp-Val sequence through an Arg-Gly-Asp-binding site of the integrin","volume":"10","author":"Choi","year":"2010","journal-title":"Proteomics"},{"key":"10.1016\/j.talanta.2026.130094_bib7","doi-asserted-by":"crossref","first-page":"3814","DOI":"10.1039\/c2an35080g","article-title":"Quantitative kinetics of proteolytic enzymes determined by a surface concentration-based assay using peptide arrays","volume":"137","author":"Jung","year":"2012","journal-title":"Analyst"},{"key":"10.1016\/j.talanta.2026.130094_bib8","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.aca.2011.12.055","article-title":"Normalization using a tagged-internal standard assay for analysis of antibody arrays and the evaluation of serological biomarkers for liver disease","volume":"718","author":"Kong","year":"2012","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.talanta.2026.130094_bib9","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1074\/mcp.R120.001936","article-title":"Developments and applications of functional protein microarrays","volume":"19","author":"Syu","year":"2020","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.talanta.2026.130094_bib10","doi-asserted-by":"crossref","first-page":"9880","DOI":"10.3390\/ijms25189880","article-title":"Mass spectrometry advancements and applications for biomarker discovery, diagnostic innovations, and personalized medicine","volume":"25","author":"Son","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.talanta.2026.130094_bib11","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s12014-024-09452-1","article-title":"Recent developments in mass-spectrometry-based targeted proteomics of clinical cancer biomarkers","volume":"21","author":"Wenk","year":"2024","journal-title":"Clin. Proteomics"},{"key":"10.1016\/j.talanta.2026.130094_bib12","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0014-5793(88)81027-5","article-title":"The complete amino acid sequence of bovine milk angiogenin","volume":"241","author":"Maes","year":"1988","journal-title":"FEBS Lett."},{"key":"10.1016\/j.talanta.2026.130094_bib13","doi-asserted-by":"crossref","first-page":"6110","DOI":"10.1021\/bi00440a057","article-title":"Amino acid sequence of bovine angiogenin","volume":"28","author":"Bond","year":"1989","journal-title":"Biochemistry"},{"key":"10.1016\/j.talanta.2026.130094_bib14","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1038\/sj.onc.1208223","article-title":"Endogenous angiogenin in endothelial cells is a general requirement for cell proliferation and angiogenesis","volume":"24","author":"Kishimoto","year":"2005","journal-title":"Oncogene"},{"key":"10.1016\/j.talanta.2026.130094_bib15","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S8756-3282(00)00332-X","article-title":"Milk basic protein: a novel protective function of milk against osteoporosis","volume":"27","author":"Toba","year":"2000","journal-title":"Bone"},{"key":"10.1016\/j.talanta.2026.130094_bib16","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.bone.2007.10.012","article-title":"Identification of angiogenin as the osteoclastic bone resorption-inhibitory factor in bovine milk","volume":"42","author":"Morita","year":"2008","journal-title":"Bone"},{"key":"10.1016\/j.talanta.2026.130094_bib17","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.nut.2017.02.003","article-title":"Milk basic protein increases ghrelin secretion and bone mineral density in rodents","volume":"39\u201340","author":"Ishida","year":"2017","journal-title":"Nutrition"},{"key":"10.1016\/j.talanta.2026.130094_bib18","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1080\/09168451.2016.1246176","article-title":"Absorption of bovine angiogenin into peripheral blood of rats orally administered milk basic protein","volume":"81","author":"Ishida","year":"2017","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"10.1016\/j.talanta.2026.130094_bib19","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.idairyj.2009.12.018","article-title":"Characterisation of the anti-microbial activity of bovine milk ribonuclease4 and ribonuclease5 (angiogenin)","volume":"20","author":"Harris","year":"2010","journal-title":"Int. Dairy J."},{"key":"10.1016\/j.talanta.2026.130094_bib20","doi-asserted-by":"crossref","first-page":"2217","DOI":"10.1016\/S0024-3205(01)01311-X","article-title":"Inhibition of human immunodeficiency virus type 1 reverse transcriptase, protease and integrase by bovine milk proteins","volume":"69","author":"Ng","year":"2001","journal-title":"Life Sci."},{"key":"10.1016\/j.talanta.2026.130094_bib21","doi-asserted-by":"crossref","first-page":"4891","DOI":"10.3168\/jds.2013-6612","article-title":"Identification of milk proteins enhancing the antimicrobial activity of lactoferrin and lactoferricin","volume":"96","author":"Murata","year":"2013","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.talanta.2026.130094_bib22","doi-asserted-by":"crossref","first-page":"257","DOI":"10.3168\/jds.2024-25493","article-title":"Effects of high-temperature, short-time pasteurization on milk and whey during commercial whey protein concentrate production","volume":"108","author":"Haas","year":"2025","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.talanta.2026.130094_bib23","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1006\/bbrc.1997.6280","article-title":"Detection, quantitation, and localization of bovine angiogenin by immunological assays","volume":"232","author":"Chang","year":"1997","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.talanta.2026.130094_bib24","doi-asserted-by":"crossref","first-page":"6282","DOI":"10.1021\/bi00417a013","article-title":"Isolation of bovine angiogenin using a placental ribonuclease inhibitor binding assay","volume":"27","author":"Bond","year":"1988","journal-title":"Biochemistry"},{"key":"10.1016\/j.talanta.2026.130094_bib25","doi-asserted-by":"crossref","DOI":"10.1155\/2019\/5214821","article-title":"Misleading westerns: common quantification mistakes in western blot densitometry and proposed corrective measures","volume":"2019","author":"Butler","year":"2019","journal-title":"Biomed Res. Int."},{"key":"10.1016\/j.talanta.2026.130094_bib26","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1111\/sms.12702","article-title":"An overview of technical considerations for western blotting applications to physiological research","volume":"27","author":"Bass","year":"2017","journal-title":"Scand. J. Med. Sci. Sports"},{"key":"10.1016\/j.talanta.2026.130094_bib27","doi-asserted-by":"crossref","DOI":"10.1177\/03000605251315913","article-title":"An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids","volume":"53","author":"Aydin","year":"2025","journal-title":"J. Int. Med. Res."},{"key":"10.1016\/j.talanta.2026.130094_bib28","doi-asserted-by":"crossref","DOI":"10.1002\/ansa.202400007","article-title":"Challenges and recent advances in quantitative mass spectrometry-based metabolomics","volume":"5","author":"Ghafari","year":"2024","journal-title":"Anal. Sci. Adv."},{"key":"10.1016\/j.talanta.2026.130094_bib29","article-title":"Quantitative mass spectrometry: an overview","volume":"374","author":"Urban","year":"2016","journal-title":"Philos. Trans. A Math. Phys. Eng. Sci."},{"key":"10.1016\/j.talanta.2026.130094_bib30","doi-asserted-by":"crossref","first-page":"6364","DOI":"10.1039\/D2RA00058J","article-title":"Recent advances in fluorescence anisotropy\/polarization signal amplification","volume":"12","author":"Xiao","year":"2022","journal-title":"RSC Adv."},{"key":"10.1016\/j.talanta.2026.130094_bib31","doi-asserted-by":"crossref","first-page":"7132","DOI":"10.3390\/s20247132","article-title":"Fluorescence polarization-based bioassays: new horizons","volume":"20","author":"Hendrickson","year":"2020","journal-title":"Sensors"},{"key":"10.1016\/j.talanta.2026.130094_bib32","doi-asserted-by":"crossref","first-page":"4712","DOI":"10.3390\/molecules29194712","article-title":"Fluorescence polarization assay for infection diagnostics: a review","volume":"29","author":"Eremin","year":"2024","journal-title":"Molecules"},{"key":"10.1016\/j.talanta.2026.130094_bib33","doi-asserted-by":"crossref","first-page":"6808","DOI":"10.1039\/D1AN01483H","article-title":"An electrochemical aptasensor for the milk allergen beta-lactoglobulin detection based on a target-induced nicking site reconstruction strategy","volume":"146","author":"Qiu","year":"2021","journal-title":"Analyst"},{"key":"10.1016\/j.talanta.2026.130094_bib34","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.1021\/acs.jafc.1c06350","article-title":"Electrochemical methods for the analysis of milk","volume":"70","author":"Motshakeri","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.talanta.2026.130094_bib35","doi-asserted-by":"crossref","first-page":"9715","DOI":"10.1007\/s13399-021-01766-6","article-title":"Synthesis and characterization of graphene derived from biomass for optical sensing of milk proteins","volume":"13","author":"Thiruganasambanthan","year":"2023","journal-title":"Biomass Convers. Biorefin."},{"key":"10.1016\/j.talanta.2026.130094_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2023.124366","article-title":"A portable smartphone-based imaging surface plasmon resonance biosensor for allergen detection in plant-based milks","volume":"257","author":"Xiao","year":"2023","journal-title":"Talanta"},{"key":"10.1016\/j.talanta.2026.130094_bib37","doi-asserted-by":"crossref","first-page":"3039","DOI":"10.1039\/D4AY00460D","article-title":"A rapid and sensitive aptamer-based biosensor for beta-lactoglobulin in milk","volume":"16","author":"Liu","year":"2024","journal-title":"Anal. Methods"},{"key":"10.1016\/j.talanta.2026.130094_bib38","doi-asserted-by":"crossref","first-page":"98","DOI":"10.3390\/separations9040098","article-title":"Development of a high-throughput screening analysis for 195 pesticides in raw milk by modified QuEChERS sample preparation and liquid chromatography quadrupole time-of-flight mass spectrometry","volume":"9","author":"Wu","year":"2022","journal-title":"Separations"},{"key":"10.1016\/j.talanta.2026.130094_bib39","doi-asserted-by":"crossref","DOI":"10.1088\/2050-6120\/2\/3\/032001","article-title":"Exploiting fluorescence for multiplex immunoassays on protein microarrays","volume":"2","author":"Herb\u00e1th","year":"2014","journal-title":"Methods Appl. Fluoresc."},{"key":"10.1016\/j.talanta.2026.130094_bib40","doi-asserted-by":"crossref","first-page":"256","DOI":"10.3390\/s17020256","article-title":"Analytical protein microarrays: advancements towards clinical applications","volume":"17","author":"Sauer","year":"2017","journal-title":"Sensors"},{"key":"10.1016\/j.talanta.2026.130094_bib41","doi-asserted-by":"crossref","first-page":"228","DOI":"10.3390\/microarrays4020228","article-title":"Label and label-free detection techniques for protein microarrays","volume":"4","author":"Syahir","year":"2015","journal-title":"Microarrays"},{"key":"10.1016\/j.talanta.2026.130094_bib42","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1023\/B:BILE.0000044452.57375.fb","article-title":"High level production of bovine angiogenin in E. coli by an efficient refolding procedure","volume":"26","author":"Jang","year":"2004","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.talanta.2026.130094_bib43","doi-asserted-by":"crossref","first-page":"3849","DOI":"10.1021\/ac300414g","article-title":"High-throughput analysis of protein\u2212protein interactions in picoliter-volume droplets using fluorescence polarization","volume":"84","author":"Choi","year":"2012","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2026.130094_bib44","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.bios.2014.09.013","article-title":"A droplet-based fluorescence polarization immunoassay (dFPIA) platform for rapid and quantitative analysis of biomarkers","volume":"67","author":"Choi","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.talanta.2026.130094_bib45","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.1126\/science.289.5485.1760","article-title":"Printing proteins as microarrays for high-throughput function determination","volume":"289","author":"MacBeath","year":"2000","journal-title":"Science"},{"key":"10.1016\/j.talanta.2026.130094_bib46","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1038\/nmeth.1208","article-title":"A practical guide to single-molecule FRET","volume":"5","author":"Roy","year":"2008","journal-title":"Nat. Methods"},{"key":"10.1016\/j.talanta.2026.130094_bib47","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1038\/nprot.2015.045","article-title":"Labeling proteins on live mammalian cells using click chemistry","volume":"10","author":"Niki\u0107","year":"2015","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.talanta.2026.130094_bib48","doi-asserted-by":"crossref","first-page":"4078","DOI":"10.1021\/acs.analchem.5c06619","article-title":"Beyond binding affinity: detailed profiling of protein-ligand interactions with time-resolved FRET","volume":"98","author":"Liu","year":"2026","journal-title":"Anal. Chem."},{"key":"10.1016\/j.talanta.2026.130094_bib49","doi-asserted-by":"crossref","first-page":"8721","DOI":"10.1021\/acs.analchem.4c00965","article-title":"Reverse-phase protein microarray for overexpressed Escherichia coli lysates reveal a novel tyrosine kinase","volume":"96","author":"Keskin","year":"2024","journal-title":"Anal. Chem."}],"container-title":["Talanta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914026007502?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0039914026007502?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:25:56Z","timestamp":1786695956000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0039914026007502"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":49,"alternative-id":["S0039914026007502"],"URL":"https:\/\/doi.org\/10.1016\/j.talanta.2026.130094","relation":{},"ISSN":["0039-9140"],"issn-type":[{"value":"0039-9140","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Quantitative analysis of bovine angiogenin in milk using a microliter-volume protein microarray","name":"articletitle","label":"Article Title"},{"value":"Talanta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.talanta.2026.130094","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"130094"}}